A novel role for helix 12 of retinoid X receptor in regulating repression.

abstract：:Runx1/AML1 is a transcription factor implicated in tissue stem cell regulation and belongs to the small Runx family of cancer genes. In the hair follicle (HF), Runx1 epithelial deletion in morphogenesis impairs normal adult hair homeostasis (cycle) and blocks adult hair follicle stem cells (HFSCs) in quiescence. Here,...

journal_title：Molecular and cellular biology

authors： Hoi CS,Lee SE,Lu SY,McDermitt DJ,Osorio KM,Piskun CM,Peters RM,Paus R,Tumbar T

更新日期：2010-05-01 00:00:00

abstract：:The ability of basic helix-loop-helix muscle regulatory factors (MRFs), such as MyoD, to convert nonmuscle cells to a myogenic lineage is regulated by numerous growth factor and oncoprotein signaling pathways. Previous studies have shown that H-Ras 12V inhibits differentiation to a skeletal muscle lineage by disruptin...

journal_title：Molecular and cellular biology

authors： Ramocki MB,Johnson SE,White MA,Ashendel CL,Konieczny SF,Taparowsky EJ

更新日期：1997-07-01 00:00:00

abstract：:Endonuclease decay of nonsense-containing beta-globin mRNA in erythroid cells generates 5'-truncated products that were reported previously to have a cap or caplike structure. We confirmed that this 5' modification is indistinguishable from the cap on full-length mRNA, and Western blotting, immunoprecipitation, and ac...

journal_title：Molecular and cellular biology

authors： Otsuka Y,Kedersha NL,Schoenberg DR

更新日期：2009-04-01 00:00:00

abstract：:In vitro studies have implicated nuclear respiratory factor 1 (NRF-1) in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. We investigated here the in vivo function of NRF-1 in mammals by disrupting the gene in mice. A...

journal_title：Molecular and cellular biology

authors： Huo L,Scarpulla RC

更新日期：2001-01-01 00:00:00

abstract：:Activation of adult myoblasts called satellite cells during muscle degeneration is an important aspect of muscle regeneration. Satellite cells are believed to be the only myogenic stem cells in adult skeletal muscle and the source of regenerating muscle fibers. Upon activation, satellite cells proliferate, migrate to ...

journal_title：Molecular and cellular biology

authors： Taylor JM,Dupont-Versteegden EE,Davies JD,Hassell JA,Houlé JD,Gurley CM,Peterson CA

更新日期：1997-09-01 00:00:00

abstract：:Src homology 2 (SH2) domains provide specificity to intracellular signaling by binding to specific phosphotyrosine (phospho-Tyr)-containing sequences. We recently developed a technique using a degenerate phosphopeptide library to predict the specificity of individual SH2 domains (src family members, Abl, Nck, Sem5, ph...

journal_title：Molecular and cellular biology

authors： Songyang Z,Shoelson SE,McGlade J,Olivier P,Pawson T,Bustelo XR,Barbacid M,Sabe H,Hanafusa H,Yi T

更新日期：1994-04-01 00:00:00

abstract：:The expression of the c-jun proto-oncogene is rapidly induced in response to mitogens acting on a large variety of cell surface receptors. The resulting functional activity of c-Jun proteins appears to be critical for cell proliferation. Recently, we have shown that a large family of G protein-coupled receptors (GPCRs...

journal_title：Molecular and cellular biology

authors： Marinissen MJ,Chiariello M,Pallante M,Gutkind JS

更新日期：1999-06-01 00:00:00

abstract：:We previously created a knock-in mutant mouse harboring a dominantly negative mutant thyroid hormone receptor beta (TRbeta(PV/PV) mouse) that spontaneously develops a follicular thyroid carcinoma similar to human thyroid cancer. We found that beta-catenin, which plays a critical role in oncogenesis, was highly elevate...

journal_title：Molecular and cellular biology

authors： Guigon CJ,Zhao L,Lu C,Willingham MC,Cheng SY

更新日期：2008-07-01 00:00:00

abstract：:The fucose alpha(1-->2) galactose beta structure is expressed by uterine epithelial cells in the mouse and has been implicated in blastocyst adhesion events thought to be required for murine implantation. Fucalpha(1-->2)Galbeta moieties and cognate fucosyltransferases are also expressed by epithelial cells of the male...

journal_title：Molecular and cellular biology

authors： Domino SE,Zhang L,Gillespie PJ,Saunders TL,Lowe JB

更新日期：2001-12-01 00:00:00

abstract：:BUD23 was identified from a bioinformatics analysis of Saccharomyces cerevisiae genes involved in ribosome biogenesis. Deletion of BUD23 leads to severely impaired growth, reduced levels of the small (40S) ribosomal subunit, and a block in processing 20S rRNA to 18S rRNA, a late step in 40S maturation. Bud23 belongs t...

journal_title：Molecular and cellular biology

authors： White J,Li Z,Sardana R,Bujnicki JM,Marcotte EM,Johnson AW

更新日期：2008-05-01 00:00:00

abstract：:In the early phase of bone damage, low concentrations of the cytokine tumor necrosis factor alpha (TNF-α) favor osteoblast differentiation. In contrast, chronic high doses of the same cytokine contribute to bone loss, demonstrating opposite effects depending on its concentration and on the time of exposure. In the bon...

journal_title：Molecular and cellular biology

authors： Daniele S,Natali L,Giacomelli C,Campiglia P,Novellino E,Martini C,Trincavelli ML

更新日期：2017-03-31 00:00:00

abstract：:In an effort to identify widely active positive regulatory elements, we have examined the action of the cytomegalovirus enhancer-promoter in transgenic mice. These elements activated expression in 24 of 28 tissues tested. The greatest expression was observed in the heart, kidney, brain, and testis. Maximum expression ...

journal_title：Molecular and cellular biology

authors： Schmidt EV,Christoph G,Zeller R,Leder P

更新日期：1990-08-01 00:00:00

abstract：:The Csk tyrosine kinase negatively regulates the Src family kinases Lck and Fyn in T cells. Engagement of the T-cell antigen receptor results in a removal of Csk from the lipid raft-associated transmembrane protein PAG/Cbp. Instead, Csk becomes associated with an approximately 72-kDa tyrosine-phosphorylated protein, w...

journal_title：Molecular and cellular biology

authors： Rahmouni S,Vang T,Alonso A,Williams S,van Stipdonk M,Soncini C,Moutschen M,Schoenberger SP,Mustelin T

更新日期：2005-03-01 00:00:00

abstract：:The levels of the A, B, and C subunits of protein phosphatase 2A in extracts from synchronized embryonic bovine tracheal cells were determined by immunoblotting with subunit-specific antibodies. A constant amount of each subunit was found in resting cells as well as in growing cells from all stages of the cell cycle. ...

journal_title：Molecular and cellular biology

authors： Ruediger R,Van Wart Hood JE,Mumby M,Walter G

更新日期：1991-08-01 00:00:00

abstract：:Peroxisomal matrix proteins are synthesized on cytosolic ribosomes and transported to the organelle by shuttling receptors. Matrix proteins containing a type 1 signal are carried to the peroxisome by PEX5, whereas those harboring a type 2 signal are transported by a PEX5-PEX7 complex. The pathway followed by PEX5 duri...

journal_title：Molecular and cellular biology

authors： Rodrigues TA,Alencastre IS,Francisco T,Brites P,Fransen M,Grou CP,Azevedo JE

更新日期：2014-08-01 00:00:00

abstract：:The Swi5 zinc finger and the Pho2 homeodomain DNA-binding proteins bind cooperatively to the HO promoter. Pho2 (also known as Bas2 or Grf10) activates transcription of diverse genes, acting with multiple distinct DNA-binding proteins. We have performed a genetic screen to identify amino acid residues in Swi5 that are ...

journal_title：Molecular and cellular biology

authors： Bhoite LT,Stillman DJ

更新日期：1998-11-01 00:00:00

abstract：:We have characterized a pathway for nuclear export of the glucocorticoid receptor (GR) in mammalian cells. This pathway involves the Ca2+ -binding protein calreticulin (CRT), which directly contacts the DNA binding domain (DBD) of GR and facilitates its delivery from the nucleus to the cytoplasm. In the present study,...

journal_title：Molecular and cellular biology

authors： Holaska JM,Black BE,Rastinejad F,Paschal BM

更新日期：2002-09-01 00:00:00

abstract：:It has been hypothesized that protein factors may protect CpG islands from methyltransferase during development and that demethylation may involve protein-DNA interactions at demethylated sites. However, direct evidence has been lacking. In this study, demethylation at the EBNA-1 binding sites of the Epstein-Barr viru...

journal_title：Molecular and cellular biology

authors： Hsieh CL

更新日期：1999-01-01 00:00:00

abstract：:The tumor suppressor WT1 represses and activates transcription. The loss and/or imbalance of the dual transcriptional activity of WT1 may contribute to Wilms' tumor. In this study, we identified par-4 (for prostate apoptosis response) as a WT1-interacting protein that itself functions as a transcriptional repressor. p...

journal_title：Molecular and cellular biology

authors： Johnstone RW,See RH,Sells SF,Wang J,Muthukkumar S,Englert C,Haber DA,Licht JD,Sugrue SP,Roberts T,Rangnekar VM,Shi Y

更新日期：1996-12-01 00:00:00

abstract：:The lactogenic hormones, i.e., prolactin and glucocorticoids, act in concert to stimulate transcription factors responsible for hormone-dependent milk protein gene expression. In the mammary gland, prolactin activates Stat5a and Stat5b and glucocorticoids activate the glucocorticoid receptor (GR). Immunoprecipitation ...

journal_title：Molecular and cellular biology

authors： Cella N,Groner B,Hynes NE

更新日期：1998-04-01 00:00:00

abstract：:Sequences of DNA that hybridize on Southern blots with cloned intracisternal A-particle (IAP) sequences have been examined in genomic DNAs of neonatal mice, livers of adult mice (3, 6, 12, 18, 24, and 26 months old), and the solid myeloma tumor MOPC-315. The isoschizomers HpaII (CCGG or mCCGG) and MspI (CCGG or CmCGG)...

journal_title：Molecular and cellular biology

authors： Mays-Hoopes LL,Brown A,Huang RC

更新日期：1983-08-01 00:00:00

abstract：:The gene encoding the 180-kDa DNA strand transfer protein beta from the yeast Saccharomyces cerevisiae was identified and sequenced. This gene, DST2 (DNA strand transferase 2), was located on chromosome VII. dst2 gene disruption mutants exhibited temperature-sensitive sporulation and a 50% longer generation time durin...

journal_title：Molecular and cellular biology

authors： Dykstra CC,Kitada K,Clark AB,Hamatake RK,Sugino A

更新日期：1991-05-01 00:00:00

abstract：:We examined the relationship between pre-mRNA splicing and the nuclear matrix by using an in vivo system that we have developed. Plasmids containing the inducible herpesvirus tk gene promoter linked to an intron-containing segment of the rabbit beta-globin gene were transfected into HeLa cells, and then the promoter w...

journal_title：Molecular and cellular biology

authors： Zeitlin S,Parent A,Silverstein S,Efstratiadis A

更新日期：1987-01-01 00:00:00

abstract：:Recognition of gene promoters by RNA polymerase II is mediated by general transcription factor IID (TFIID), which has been thought to be a static complex and to play a passive role in the regulation of gene expression under the instruction of gene-specific transcription factors. Here we show that transforming growth f...

journal_title：Molecular and cellular biology

authors： Nakagawa T,Hosogane M,Nakagawa M,Morohoshi A,Funayama R,Nakayama K

更新日期：2018-02-12 00:00:00

abstract：:RBM4 promotes differentiation of neuronal progenitor cells and neurite outgrowth of cultured neurons via its role in splicing regulation. In this study, we further explored the role of RBM4 in neuronal differentiation. During neuronal differentiation, energy production shifts from glycolysis to oxidative phosphorylati...

journal_title：Molecular and cellular biology

authors： Su CH,Hung KY,Hung SC,Tarn WY

更新日期：2017-01-19 00:00:00

abstract：:Posttranslational modifications (PTMs) can alter many fundamental properties of a protein. One or combinations of them have been known to regulate the dynamics of many cellular pathways and consequently regulate all vital processes. Understandably, pathogens have evolved sophisticated strategies to subvert these mecha...

journal_title：Molecular and cellular biology

authors： Verma S,Mohapatra G,Ahmad SM,Rana S,Jain S,Khalsa JK,Srikanth CV

更新日期：2015-09-01 00:00:00

abstract：:Microinjection of p21Ha-ras proteins into Xenopus laevis oocytes induces a rapid increase of 1,2-diacylglycerol (DAG) levels. The observed alterations in DAG levels were consistent with the ability of the protein to induce maturation, measured by germinal vesicle breakdown (GVBD). Both the increase in DAG levels and G...

journal_title：Molecular and cellular biology

authors： Lacal JC

更新日期：1990-01-01 00:00:00

abstract：:We isolated and characterized LP1.2, a mouse myeloma mutant with a deletion of at least 4 kilobases (kb) immediately 3' of the alpha gene and introduction of at least 5 kb of novel (nonimmunoglobulin) sequence in its place. A 6.2-kb genomic EcoRI fragment from the mutated allele was cloned, and a subfragment was seque...

journal_title：Molecular and cellular biology

authors： Gregor PD,Morrison SL

更新日期：1986-06-01 00:00:00

abstract：:Streptozotocin (STZ) is widely used to induce diabetic rodent models. It is specifically toxic to pancreatic beta cells and causes severe destruction and dysfunction. We investigated the effect of 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG) on an STZ-induced diabetic mouse model. PLAG attenuated the glucose i...

journal_title：Molecular and cellular biology

authors： Kim J,Kim JH,Sohn KY,Yoon SY,Kim JW

更新日期：2019-10-11 00:00:00

abstract：:Ribosomal protein L5 binds specifically to 5S rRNA to form a complex that is a precursor to 60S subunit assembly in vivo. Analyses in yeast cells, mammalian cells, and Xenopus embryos have shown that the accumulation of L5 is not coordinated with the expression of other ribosomal proteins. In this study, the primary s...

journal_title：Molecular and cellular biology

authors： Wormington WM

更新日期：1989-12-01 00:00:00