Abstract:
:The gene encoding the 180-kDa DNA strand transfer protein beta from the yeast Saccharomyces cerevisiae was identified and sequenced. This gene, DST2 (DNA strand transferase 2), was located on chromosome VII. dst2 gene disruption mutants exhibited temperature-sensitive sporulation and a 50% longer generation time during vegetative growth than did the wild type. Spontaneous mitotic recombination in the mutants was reduced severalfold for both intrachromosomal recombination and intragenic gene conversion. The mutants also had reduced levels of the intragenic recombination that is induced during meiosis. Meiotic recombinants were, however, somewhat unstable in the mutants, with a decrease in recombinants and survival upon prolonged incubation in sporulation media. spo13 or spo13 rad50 mutations did not relieve the sporulation defect of dst2 mutations. A dst1 dst2 double mutant has the same phenotype as a dst2 single mutant. All phenotypes associated with the dst2 mutations could be complemented by a plasmid containing DST2.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Dykstra CC,Kitada K,Clark AB,Hamatake RK,Sugino Adoi
10.1128/mcb.11.5.2583subject
Has Abstractpub_date
1991-05-01 00:00:00pages
2583-92issue
5eissn
0270-7306issn
1098-5549journal_volume
11pub_type
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