Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

abstract：:An analysis of the sizes and sequence content of nuclear RNA transcripts of the heavy-chain locus in two B-cell lymphomas, 70Z/3 and 38C-13, and in selected hybridoma derivatives of 38C has led to the identification of two distinct precursors of the mRNAs encoding the membrane and secretory forms of mu chain. These pr...

journal_title：Molecular and cellular biology

authors： Nelson KJ,Haimovich J,Perry RP

更新日期：1983-07-01 00:00:00

abstract：:The TIS11 primary response gene is rapidly and transiently induced by both 12-O-tetradecanoylphorbol-13-acetate and growth factors. The predicted TIS11 protein contains a 6-amino-acid repeat, YKTELC. We cloned two additional cDNAs, TIS11b and TIS11d, that contain the YKTELC sequence. TIS11, TIS11b, and TIS11d proteins...

journal_title：Molecular and cellular biology

authors： Varnum BC,Ma QF,Chi TH,Fletcher B,Herschman HR

更新日期：1991-03-01 00:00:00

abstract：:The TIP60 histone acetyltransferase plays diverse roles in DNA damage responses, DNA double-strand break repair, and transcriptional regulation. TIP60 resides within a multisubunit complex that has been shown to be targeted by transcription factors and to be involved in histone acetylation and transcriptional activati...

journal_title：Molecular and cellular biology

authors： Park JH,Sun XJ,Roeder RG

更新日期：2010-06-01 00:00:00

abstract：:Our recent studies implicated key and distinct roles for the highly related RalA and RalB small GTPases (82% sequence identity) in pancreatic ductal adenocarcinoma (PDAC) tumorigenesis and invasive and metastatic growth, respectively. How RalB may promote PDAC invasion and metastasis has not been determined. In light ...

journal_title：Molecular and cellular biology

authors： Neel NF,Rossman KL,Martin TD,Hayes TK,Yeh JJ,Der CJ

更新日期：2012-04-01 00:00:00

abstract：:Chronic inflammation plays important roles in cancer initiation and progression. Resolving chronic inflammation or blocking inflammatory signal transduction may prevent cancer development. Here, we report that the combined low-dose use of two anti-inflammatory drugs, aspirin and triptolide, reduces spontaneous lung ca...

journal_title：Molecular and cellular biology

authors： Zheng L,Jia J,Dai H,Wan L,Liu J,Hu L,Zhou M,Qiu M,Chen X,Chang L,Kim JY,Reckamp K,Raz DJ,Xia Z,Shen B

更新日期：2017-07-14 00:00:00

abstract：:The oncogene product of the avian sarcoma virus CT10, P47gag-crk, contains the SH2, SH2', and SH3 domains and binds proteins in a phosphotyrosine (ptyr)-dependent manner. In this study, we have determined the region of P47gag-crk essential for binding to ptyr-containing proteins. Mutant P47gag-crk proteins expressed i...

journal_title：Molecular and cellular biology

authors： Matsuda M,Mayer BJ,Hanafusa H

更新日期：1991-03-01 00:00:00

abstract：:A new transforming gene has been molecularly cloned from hamster SHOK cells transformed with DNA extracted from a human thyroid carcinoma cell line and named the cot (cancer Osaka thyroid) oncogene. cDNA sequencing disclosed that this oncogene codes for a protein with 415 amino acid residues, and computer matching sho...

journal_title：Molecular and cellular biology

authors： Miyoshi J,Higashi T,Mukai H,Ohuchi T,Kakunaga T

更新日期：1991-08-01 00:00:00

abstract：:In heat-shocked tomato cell cultures, cytoplasmic heat shock granules (HSGs) are tightly associated with a specific subset of mRNAs coding mainly for the untranslated control proteins. This messenger ribonucleoprotein complex was banded in a CsCl gradient after fixation with formaldehyde (approximately 1.30 g/cm3). It...

journal_title：Molecular and cellular biology

authors： Nover L,Scharf KD,Neumann D

更新日期：1989-03-01 00:00:00

abstract：:A genomic DNA fragment containing the Raf-1 promoter region was isolated by using a cDNA extension clone. Nucleotide sequencing of genomic DNA clones, primer extension, and S1 nuclease assays have been used to identify the 5' ends of Raf-1 RNAs. Consistent with its ubiquitous expression, the Raf-1 promoter region had ...

journal_title：Molecular and cellular biology

authors： Beck TW,Brennscheidt U,Sithanandam G,Cleveland J,Rapp UR

更新日期：1990-07-01 00:00:00

abstract：:Nitric oxide (NO) has been thought to regulate the immune system through S nitrosylation of the transcriptional factor NF-kappaB. However, regulatory effects of NO on innate immune responses are unclear. Here, we report that NO has a capability to control Toll-like receptor-mediated signaling through S nitrosylation. ...

journal_title：Molecular and cellular biology

authors： Into T,Inomata M,Nakashima M,Shibata K,Häcker H,Matsushita K

更新日期：2008-02-01 00:00:00

abstract：:We investigated the role of cytoplasmic and anchor domains of type II glycoproteins in intracellular transport, oligomerization, and endocytosis by expressing the wild-type and chimeric genes in mammalian cells. Chimeric genes were constructed by exchanging the DNA segments that encode the cytoplasmic and anchor domai...

journal_title：Molecular and cellular biology

authors： Kundu A,Jabbar MA,Nayak DP

更新日期：1991-05-01 00:00:00

abstract：:A 215-base-pair (bp) region of the mouse MOPC 41 kappa light-chain immunoglobulin gene enhancer has been analyzed for specific binding of lymphoid and nonlymphoid nuclear factors. Mobility shift assays with a series of overlapping DNA fragments have mapped DNA-binding sites for three unique factors. The B-cell-specifi...

journal_title：Molecular and cellular biology

authors： Currie RA,Roeder RG

更新日期：1989-10-01 00:00:00

abstract：:mof6-1 was originally isolated as a recessive mutation in Saccharomyces cerevisiae which promoted increased efficiencies of programmed -1 ribosomal frameshifting and rendered cells unable to maintain the killer virus. Here, we demonstrate that mof6-1 is a unique allele of the histone deacetylase RPD3, that the deacety...

journal_title：Molecular and cellular biology

authors： Meskauskas A,Baxter JL,Carr EA,Yasenchak J,Gallagher JE,Baserga SJ,Dinman JD

更新日期：2003-03-01 00:00:00

abstract：:The inhibition of receptor binding of [3H]phorbol-12,13-dibutyrate (PDBu) by a factor from human serum was characterized. The serum factor inhibited [3H]PDBu binding in intact monolayer cultures of the rat embryo cell line CREF N and in a subcellular system containing membranes from these cells. Inhibition occurred at...

journal_title：Molecular and cellular biology

authors： Horowitz AD,Greenebaum E,Nicolaides M,Woodward K,Weinstein IB

更新日期：1982-05-01 00:00:00

abstract：:The Ras-RAF-mitogen-activated protein kinase (Ras-RAF-MAPK) pathway is overactive in many cancers and in some developmental disorders. In one of those disorders, namely, Noonan syndrome, nine activating C-RAF mutations cluster around Ser(259), a regulatory site for inhibition by 14-3-3 proteins. We show that these mut...

journal_title：Molecular and cellular biology

authors： Molzan M,Schumacher B,Ottmann C,Baljuls A,Polzien L,Weyand M,Thiel P,Rose R,Rose M,Kuhenne P,Kaiser M,Rapp UR,Kuhlmann J,Ottmann C

更新日期：2010-10-01 00:00:00

abstract：:Low oxygen gradients (hypoxia and anoxia) are important determinants of pathological conditions under which the tissue blood supply is deficient or defective, such as in solid tumors. We have been investigating the relationship between the activation of hypoxia-inducible factor 1 (HIF-1), the primary transcriptional r...

journal_title：Molecular and cellular biology

authors： Laderoute KR,Amin K,Calaoagan JM,Knapp M,Le T,Orduna J,Foretz M,Viollet B

更新日期：2006-07-01 00:00:00

abstract：:Two mutator genes of mammalian cells were demonstrated. One was associated with the ribonucleoside diphosphate reductase, and the other was associated with an extreme adenosine sensitivity. ...

journal_title：Molecular and cellular biology

authors： Chan VL,Guttman S,Juranka P

更新日期：1981-06-01 00:00:00

abstract：:Nucleolin is a ubiquitous multifunctional protein involved in preribosome assembly and associated with both nucleolar chromatin in interphase and nucleolar organizer regions on metaphasic chromosomes in mitosis. Extensive nucleolin phosphorylation by a casein kinase (CKII) occurs on serine in growing cells. Here we re...

journal_title：Molecular and cellular biology

authors： Belenguer P,Caizergues-Ferrer M,Labbé JC,Dorée M,Amalric F

更新日期：1990-07-01 00:00:00

abstract：:The protein tyrosine kinase Syk couples the B-cell receptor (BCR) for antigen to multiple intracellular signaling pathways and also modulates cellular responses to inducers of oxidative stress in a receptor-independent fashion. In B cells, Syk is found in both the nuclear and cytoplasmic compartments but contains no r...

journal_title：Molecular and cellular biology

authors： Zhou F,Hu J,Ma H,Harrison ML,Geahlen RL

更新日期：2006-05-01 00:00:00

abstract：:Focal adhesion kinase (FAK) is a nonreceptor protein tyrosine kinase involved in integrin-mediated control of cell behavior. Following cell adhesion to components of the extracellular matrix, FAK becomes phosphorylated at multiple sites, including tyrosines 397, 576, and 577. Tyr-397 is an autophosphorylation site tha...

journal_title：Molecular and cellular biology

authors： Owen JD,Ruest PJ,Fry DW,Hanks SK

更新日期：1999-07-01 00:00:00

abstract：:Simultaneous resistance to an array of drugs with different cytotoxic activities is a property of Saccharomyces cerevisiae, in which the protein Pdr3p has recently been shown to play a role as a transcriptional regulator. We provide evidence that the yeast PDR3 gene, which encodes a zinc finger transcription factor im...

journal_title：Molecular and cellular biology

authors： Delahodde A,Delaveau T,Jacq C

更新日期：1995-08-01 00:00:00

abstract：:TFIID is a multiprotein complex consisting of the TATA box binding protein and multiple tightly associated proteins (TAFIIs) that are required for transcription by selected activators. We previously reported cloning and partial characterization of human TAFII130 (hTAFII130). The central domain of hTAFII130 contains fo...

journal_title：Molecular and cellular biology

authors： Saluja D,Vassallo MF,Tanese N

更新日期：1998-10-01 00:00:00

abstract：:ADAR2 is a double-stranded-RNA-specific adenosine deaminase involved in the editing of mammalian RNAs by the site-selective conversion of adenosine to inosine. Previous studies from our laboratory have demonstrated that ADAR2 can modify its own pre-mRNA to create a proximal 3' splice site containing a noncanonical ade...

journal_title：Molecular and cellular biology

authors： Feng Y,Sansam CL,Singh M,Emeson RB

更新日期：2006-01-01 00:00:00

abstract：:RBM4 promotes differentiation of neuronal progenitor cells and neurite outgrowth of cultured neurons via its role in splicing regulation. In this study, we further explored the role of RBM4 in neuronal differentiation. During neuronal differentiation, energy production shifts from glycolysis to oxidative phosphorylati...

journal_title：Molecular and cellular biology

authors： Su CH,Hung KY,Hung SC,Tarn WY

更新日期：2017-01-19 00:00:00

abstract：:Exchange proteins activated by cAMP (cyclic AMP) 2 (Epac2) is a guanine nucleotide exchange factor for Rap1, a small G protein involved in many cellular functions, including cell adhesion, differentiation, and exocytosis. Epac2 interacts with Ras-GTP via a Ras association (RA) domain. Previous studies have suggested t...

journal_title：Molecular and cellular biology

authors： Liu C,Takahashi M,Li Y,Song S,Dillon TJ,Shinde U,Stork PJ

更新日期：2008-12-01 00:00:00

abstract：:Aurora A is a mitotic kinase that localizes to centrosomes. Expression of this protein is normally limited to the mitotic stage (G(2)-M) of the cell cycle, whereas human cancer cells frequently exhibit overexpression of Aurora A protein regardless of the cell cycle stage. In the present study, Aurora A transgenic mous...

journal_title：Molecular and cellular biology

authors： Fukuda T,Mishina Y,Walker MP,DiAugustine RP

更新日期：2005-06-01 00:00:00

abstract：:Signal transducer and activator of transcription 6 (Stat6) and NF-kappaB are widely distributed transcription factors which are induced by different stimuli and bind to distinct DNA sequence motifs. Interleukin-4 (IL-4), which activates Stat6, synergizes with activators of NF-kappaB to induce IL-4-responsive genes, bu...

journal_title：Molecular and cellular biology

authors： Shen CH,Stavnezer J

更新日期：1998-06-01 00:00:00

abstract：:The failure to transcribe the phenylalanine hydroxylase (PAH) gene in the liver of hepatocyte nuclear factor 1alpha (HNF1alpha)-deficient mice correlated with DNA hypermethylation and the presence of an inactive chromatin structure (M. Pontoglio, D. M. Faust, A. Doyen, M. Yaniv, and M. C. Weiss, Mol. Cell. Biol. 17:49...

journal_title：Molecular and cellular biology

authors： Viollet B,Yaniv M,Pontoglio M

更新日期：2001-06-01 00:00:00

abstract：:We have identified a gene that encodes the polypeptide cytochrome b in the avian malarial parasite Plasmodium gallinaceum. The gene containing the open reading frame was found to be located on a 6.2-kilobase multimeric extrachromosomal element. The amino acid translation from this gene demonstrated significant similar...

journal_title：Molecular and cellular biology

authors： Aldritt SM,Joseph JT,Wirth DF

更新日期：1989-09-01 00:00:00

abstract：:A major goal of cancer research is the identification of tumor-specific vulnerabilities that can be exploited for the development of therapies that are selectively toxic to the tumor. We show here that the transcriptional coactivators peroxisome proliferator-activated receptor gamma coactivator 1β (PGC1β) and estrogen...

journal_title：Molecular and cellular biology

authors： Fisher KW,Das B,Kim HS,Clymer BK,Gehring D,Smith DR,Costanzo-Garvey DL,Fernandez MR,Brattain MG,Kelly DL,MacMillan J,White MA,Lewis RE

更新日期：2015-11-01 00:00:00