Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast.

abstract：:AIRE is a transcriptional activator that directs the ectopic expression of many tissue-specific genes in medullary thymic epithelial cells, which plays an important role in the negative selection of autoreactive T cells. However, its mechanism of action remains poorly understood. In this study, we found that AIRE regu...

journal_title：Molecular and cellular biology

authors： Oven I,Brdicková N,Kohoutek J,Vaupotic T,Narat M,Peterlin BM

更新日期：2007-12-01 00:00:00

abstract：:Simple sequence repeat telomeric DNA is maintained by a specialized reverse transcriptase, telomerase. The integral RNA subunit of telomerase contains a template region that determines the sequence added to chromosome ends. Aside from providing the template, little is known about the role of the telomerase RNA. In add...

journal_title：Molecular and cellular biology

authors： Mitchell JR,Cheng J,Collins K

更新日期：1999-01-01 00:00:00

abstract：:To investigate the transcriptional program underlying thyroid hormone (T3)-induced cell proliferation, cDNA microarrays were used to survey the temporal expression profiles of 4,400 genes. Of 358 responsive genes identified, 88% had not previously been reported to be transcriptionally or functionally modulated by T3. ...

journal_title：Molecular and cellular biology

authors： Miller LD,Park KS,Guo QM,Alkharouf NW,Malek RL,Lee NH,Liu ET,Cheng SY

更新日期：2001-10-01 00:00:00

abstract：:sra5 mutations in Saccharomyces cerevisiae were previously shown to suppress the inefficient growth of ras2 strains on nonfermentable carbon sources and to result in deficient low-Km cyclic AMP (cAMP) phosphodiesterase activity. We have cloned SRA5 by complementation. It maps to the right arm of chromosome XV, tightly...

journal_title：Molecular and cellular biology

authors： Wilson RB,Tatchell K

更新日期：1988-01-01 00:00:00

abstract：:The human Ki-ras gene was previously reported to contain two alternative fourth exons which encode two distinct p21 proteins differing only at their carboxy termini. The present study shows that either p21 protein is able on its own to transform NIH 3T3 cells to a tumorigenic state. ...

journal_title：Molecular and cellular biology

authors： McCoy MS,Weinberg RA

更新日期：1986-04-01 00:00:00

abstract：:The expression of three different actin genes in the sea urchin, Strongylocentrotus purpuratus, was monitored in embryos and adult tissues by using untranslated mRNA sequences as specific hybridization probes. Three distinct patterns of expression were found: muscle specific, embryo specific, and constitutive (i.e., p...

journal_title：Molecular and cellular biology

authors： Garcia R,Paz-Aliaga B,Ernst SG,Crain WR Jr

更新日期：1984-05-01 00:00:00

abstract：:Mutant strain d48 of Paramecium tetraurelia lacks the gene for antigen A in the macronucleus, whereas this gene is present in the micronucleus. Transfer of macronucleoplasm from the wild type to strain d48 caused d48 to revert to the wild type after autogamy. Transfer of cytoplasm was not as effective as transfer of m...

journal_title：Molecular and cellular biology

authors： Harumoto T

更新日期：1986-10-01 00:00:00

abstract：:The E2F transcription factors are key downstream targets of the retinoblastoma protein (pRB) tumor suppressor. We have previously shown that E2F3 plays a critical role in mediating the mitogen-induced activation of E2F-responsive genes and contributes to both the inappropriate proliferation and the p53-dependent apopt...

journal_title：Molecular and cellular biology

authors： Ziebold U,Lee EY,Bronson RT,Lees JA

更新日期：2003-09-01 00:00:00

abstract：:To define the role of Irx4, a member of the Iroquois family of homeobox transcription factors in mammalian heart development and function, we disrupted the murine Irx4 gene. Cardiac morphology in Irx4-deficient mice (designated Irx4(Delta ex2/Delta ex2)) was normal during embryogenesis and in early postnatal life. Adu...

journal_title：Molecular and cellular biology

authors： Bruneau BG,Bao ZZ,Fatkin D,Xavier-Neto J,Georgakopoulos D,Maguire CT,Berul CI,Kass DA,Kuroski-de Bold ML,de Bold AJ,Conner DA,Rosenthal N,Cepko CL,Seidman CE,Seidman JG

更新日期：2001-03-01 00:00:00

abstract：:The adenovirus early-region 1B 19,000-molecular-weight tumor antigen is required for oncogenic transformation of cells by adenovirus. We have demonstrated that this tumor antigen is located in the nuclear envelope of infected and transformed cells and that a fraction of the protein within the nuclear envelope is assoc...

journal_title：Molecular and cellular biology

authors： White E,Blose SH,Stillman BW

更新日期：1984-12-01 00:00:00

abstract：:In eukaryotic cells, calcium entry across the cell surface activates nuclear gene expression, a process critically important for cell growth and differentiation, learning, and memory and immune cell functions. In immune cells, calcium entry occurs through store-operated Ca(2+) release-activated Ca(2+) (CRAC) channels,...

journal_title：Molecular and cellular biology

authors： Yeh YC,Parekh AB

更新日期：2015-04-01 00:00:00

abstract：:Saccharomyces cerevisiae contains eight members of a novel and fungus-specific family of bZIP proteins that is defined by four atypical residues on the DNA-binding surface. Two of these proteins, Yap1 and Yap2, are transcriptional activators involved in pleiotropic drug resistance. Although initially described as AP-1...

journal_title：Molecular and cellular biology

authors： Fernandes L,Rodrigues-Pousada C,Struhl K

更新日期：1997-12-01 00:00:00

abstract：:Using the method of chromosomal walking, we have isolated a contiguous region of the Drosophila melanogaster X chromosome which corresponds to salivary gland chromosome bands 3C12 to 3D4. This five-band region contains approximately 100 kilobases of DNA, including those sequences comprising dunce, a gene which functio...

journal_title：Molecular and cellular biology

authors： Davis RL,Davidson N

更新日期：1984-02-01 00:00:00

abstract：:During development and erythropoiesis, globin gene expression is finely modulated through an important network of transcription factors and chromatin modifying activities. In this report we provide in vivo evidence that endogenous Ikaros is recruited to the human beta-globin locus and targets the histone deacetylase H...

journal_title：Molecular and cellular biology

authors： Bottardi S,Ross J,Bourgoin V,Fotouhi-Ardakani N,Affar el B,Trudel M,Milot E

更新日期：2009-03-01 00:00:00

abstract：:Alpha-catenin, an integral part of cadherin-catenin adhesion complexes, is a major binding partner of beta-catenin, a key component of the Wnt pathway, which activates T-cell factor (TCF)/lymphoid enhancer factor (LEF) transcription and is often upregulated in cancers. Recently, we identified an alpha-catenin-related ...

journal_title：Molecular and cellular biology

authors： Merdek KD,Nguyen NT,Toksoz D

更新日期：2004-03-01 00:00:00

abstract：:The Rb protein is known to exert its activity at decision points in the G1 phase of the cell cycle. To investigate whether it may also play some role(s) at later points in the cell cycle, we used a system of rapid inducible gene amplification to conditionally overexpress Rb protein during G2 phase. A cell line express...

journal_title：Molecular and cellular biology

authors： Karantza V,Maroo A,Fay D,Sedivy JM

更新日期：1993-11-01 00:00:00

abstract：:Ribosome biogenesis is a highly regulated process ensuring that cell growth (increase in biomass) is coordinated with cell proliferation. The formation of eukaryotic ribosomes is a multistep process initiated by the transcription and processing of rRNA in the nucleolus. Concomitant with this, several preribosomal part...

journal_title：Molecular and cellular biology

authors： Castle CD,Cassimere EK,Lee J,Denicourt C

更新日期：2010-09-01 00:00:00

abstract：:HMGN proteins promote chromatin unfolding, enhance access to nucleosomes, and modulate transcription from chromatin templates. It is not known whether they act indiscriminately as general modulators of transcription or whether they regulate specific gene expression. Here, we investigated the role of HMGN3, a recently ...

journal_title：Molecular and cellular biology

authors： West KL,Castellini MA,Duncan MK,Bustin M

更新日期：2004-05-01 00:00:00

abstract：:The Wnt signaling pathway plays a pivotal role in vertebrate early development and morphogenesis. Duplin (axis duplication inhibitor) interacts with beta-catenin and prevents its binding to Tcf, thereby inhibiting downstream Wnt signaling. Here we show that Duplin is expressed predominantly from early- to mid-stage mo...

journal_title：Molecular and cellular biology

authors： Nishiyama M,Nakayama K,Tsunematsu R,Tsukiyama T,Kikuchi A,Nakayama KI

更新日期：2004-10-01 00:00:00

abstract：:Acidic fibroblast growth factor (FGF) and related family members regulate differentiation in organisms as diverse as Xenopus laevis and mammals. We utilized a well-characterized model of myogenic development to directly assess the importance of endogenously produced FGF in controlling differentiation. A role for endog...

journal_title：Molecular and cellular biology

authors： Fox JC,Hsu AY,Swain JL

更新日期：1994-06-01 00:00:00

abstract：:The role of chromatin-remodeling factors in transcription is well established, but the link between chromatin-remodeling complexes and DNA repair remains unexplored. Human Rvb1 and Rvb2 are highly conserved AAA(+) ATP binding proteins that are part of various chromatin-remodeling complexes, such as Ino80, SNF2-related...

journal_title：Molecular and cellular biology

authors： Jha S,Shibata E,Dutta A

更新日期：2008-04-01 00:00:00

abstract：:Microtubules in yeast are essential components of the mitotic and meiotic spindles and are essential for nuclear movement during cell division and mating. The relative importance in these processes of the two divergent alpha-tubulin genes of the budding yeast Saccharomyces cerevisiae, TUB1 and TUB3, was examined throu...

journal_title：Molecular and cellular biology

authors： Schatz PJ,Solomon F,Botstein D

更新日期：1986-11-01 00:00:00

abstract：:Yju2 is an essential splicing factor required for the first catalytic step after the action of Prp2. We dissected the structure of Yju2 and found that the amino (Yju2-N) and carboxyl (Yju2-C) halves of the protein can be separated and reconstituted for Yju2 function both in vivo and in vitro. Yju2-N has a weak affinit...

journal_title：Molecular and cellular biology

authors： Chiang TW,Cheng SC

更新日期：2013-05-01 00:00:00

abstract：:Posttranscriptional and posttranslational modification of macromolecules is known to fine-tune their functions. Trm112 is unique, acting as an activator of both tRNA and protein methyltransferases. Here we report that in Saccharomyces cerevisiae, Trm112 is required for efficient ribosome synthesis and progression thro...

journal_title：Molecular and cellular biology

authors： Figaro S,Wacheul L,Schillewaert S,Graille M,Huvelle E,Mongeard R,Zorbas C,Lafontaine DL,Heurgué-Hamard V

更新日期：2012-06-01 00:00:00

abstract：:The female-sterile ovarian tumor gene, otu, is located in cytological region 7F1 on the Drosophila melanogaster chromosome map. We have mapped the gene at the molecular level by using four dysgenic alleles and two revertant derivatives of these alleles as well as an ethyl methanesulfonate-induced allele. The insertion...

journal_title：Molecular and cellular biology

authors： Mulligan PK,Mohler JD,Kalfayan LJ

更新日期：1988-04-01 00:00:00

abstract：:Mxi2 is a p38alpha splice isoform that is distinctively activated by mitogenic stimuli. Here we show that Mxi2 immunoprecipitates carry a kinase activity that is persistently activated by epidermal growth factor in a fashion regulated by Ras, Raf, and MEK. We demonstrate that this kinase activity can be attributed not...

journal_title：Molecular and cellular biology

authors： Sanz-Moreno V,Casar B,Crespo P

更新日期：2003-05-01 00:00:00

abstract：:Human CRK protein is a homolog of the chicken v-crk oncogene product and consists mostly of src homology region 2 (SH2) and SH3, which are shared by many proteins, in particular those involved in signal transduction. SH2 has been shown to bind specifically to phosphotyrosine-containing peptides. We report here that bo...

journal_title：Molecular and cellular biology

authors： Tanaka S,Hattori S,Kurata T,Nagashima K,Fukui Y,Nakamura S,Matsuda M

更新日期：1993-07-01 00:00:00

abstract：:Binding of the thyroid hormone receptor (TR) to thyroid hormone-responsive elements (TREs) is crucial for regulation of gene expression by thyroid hormone. The TR binds to each half-site of a palindromic TRE separately, as a monomer, or simultaneously, as a homodimer. In addition, the TR monomer interacts with a 42-kD...

journal_title：Molecular and cellular biology

authors： Lazar MA,Berrodin TJ,Harding HP

更新日期：1991-10-01 00:00:00

abstract：:Efficient cleavage of both forms of eukaryotic initiation factor 4G (eIF4G-1 and eIF4G-2) has been achieved in HeLa cells by incubation with hybrid proteins containing poliovirus 2Apro. Entry of these proteins into cells is promoted by adenovirus particles. Substantial levels of ongoing translation on preexisting cell...

journal_title：Molecular and cellular biology

authors： Novoa I,Carrasco L

更新日期：1999-04-01 00:00:00

abstract：:Much of our knowledge on adipogenesis comes from cell culture models of preadipocyte differentiation. Adipogenesis is induced by treating confluent preadipocytes with the adipogenic cocktail, which activates transcription factors (TFs) glucocorticoid receptor (GR) and CREB within minutes and increases expression of TF...

journal_title：Molecular and cellular biology

authors： Park YK,Wang L,Giampietro A,Lai B,Lee JE,Ge K

更新日期：2017-01-04 00:00:00