The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation.

abstract：:A 249-nucleotide coding region instability determinant (CRD) destabilizes c-myc mRNA. Previous experiments identified a CRD-binding protein (CRD-BP) that appears to protect the CRD from endonuclease cleavage. However, it was unclear why a CRD-BP is required to protect a well-translated mRNA whose coding region is cove...

journal_title：Molecular and cellular biology

authors： Lemm I,Ross J

更新日期：2002-06-01 00:00:00

abstract：:Progression through mitosis requires the inactivation of the protein kinase activity of the p34cdc2-cyclin complex by a mechanism involving the degradation of cyclin. We have examined the stability in Xenopus egg extracts of radiolabeled Xenopus or sea urchin B-type cyclins synthesized in reticulocyte lysates. Xenopus...

journal_title：Molecular and cellular biology

authors： van der Velden HM,Lohka MJ

更新日期：1993-03-01 00:00:00

abstract：:Metallothioneins (MTs) are short, cysteine-rich proteins for heavy metal homeostasis and detoxification; they bind a variety of heavy metals and also act as radical scavengers. Transcription of mammalian MT genes is activated by heavy metal load via the metal-responsive transcription factor 1 (MTF-1), an essential zin...

journal_title：Molecular and cellular biology

authors： Zhang B,Egli D,Georgiev O,Schaffner W

更新日期：2001-07-01 00:00:00

abstract：:Previously we demonstrated that the heat shock transcription factor Hsf1 is indispensable for transformation of mammary epithelial cells by the Her2 oncogene. Since Hsf1 affects oncogene-induced senescence (OIS), these findings suggest that Hsf1 affects tumor initiation when OIS plays a role. Indeed, here we report th...

journal_title：Molecular and cellular biology

authors： Gabai VL,Meng L,Kim G,Mills TA,Benjamin IJ,Sherman MY

更新日期：2012-03-01 00:00:00

abstract：:Smad proteins play a key role in the intracellular signaling of the transforming growth factor beta (TGF-beta) superfamily of extracellular polypeptides that initiate signaling from the cell surface through serine/threonine kinase receptors. A subclass of Smad proteins, including Smad6 and Smad7, has been shown to fun...

journal_title：Molecular and cellular biology

authors： Datta PK,Moses HL

更新日期：2000-05-01 00:00:00

abstract：:Nuclear receptors (NRs) regulate diverse physiological processes, including the central nervous system control of energy balance. However, the molecular mechanisms for the central actions of NRs in energy balance remain relatively poorly defined. Here we report a hypothalamic gene network involving two NRs, neuron-der...

journal_title：Molecular and cellular biology

authors： Kim SG,Lee B,Kim DH,Kim J,Lee S,Lee SK,Lee JW

更新日期：2013-10-01 00:00:00

abstract：:Ure2p of Saccharomyces cerevisiae normally functions in blocking utilization of a poor nitrogen source when a good nitrogen source is available. The non-Mendelian genetic element [URE3] is a prion (infectious protein) form of Ure2p, so that overexpression of Ure2p induces the de novo appearance of infectious [URE3]. E...

journal_title：Molecular and cellular biology

authors： Maddelein ML,Wickner RB

更新日期：1999-06-01 00:00:00

abstract：:Hox gene functions are intimately linked to correct developmental expression of the genes. The identification of cis-acting regulatory sequences and their associated trans-acting factors constitutes a key step in deciphering the mechanisms underlying the correct positioning of the functional domain of Hox genes along ...

journal_title：Molecular and cellular biology

authors： Tabariès S,Lapointe J,Besch T,Carter M,Woollard J,Tuggle CK,Jeannotte L

更新日期：2005-02-01 00:00:00

abstract：:Ubiquitin is a multifunctional 76-amino-acid protein which plays critical roles in many aspects of cellular metabolism. In Caenorhabditis elegans, the major source of ubiquitin RNA is the polyubiquitin locus, UbiA. UbiA is transcribed as a polycistronic mRNA which contains 11 tandem repeats of ubiquitin sequence and p...

journal_title：Molecular and cellular biology

authors： Graham RW,Jones D,Candido EP

更新日期：1989-01-01 00:00:00

abstract：:NIH 3T3 cells infected with Moloney murine leukemia virus (MoMLV) express high levels of virus-specific RNA. To inhibit replication of the virus, we stably introduced chimeric tRNA genes encoding antisense templates into NIH 3T3 cells via a retroviral vector. Efficient expression of hybrid tRNA-MoMLV antisense transcr...

journal_title：Molecular and cellular biology

authors： Sullenger BA,Lee TC,Smith CA,Ungers GE,Gilboa E

更新日期：1990-12-01 00:00:00

abstract：:We have determined the complete nucleotide sequences of two chicken beta-tubulin genes, beta 1 and beta 2. These genes display an unusual pattern of segmental homology which indicates that they originally arose by gene duplication and have subsequently coevolved by a process that included localized gene conversion or ...

journal_title：Molecular and cellular biology

authors： Sullivan KF,Lau JT,Cleveland DW

更新日期：1985-09-01 00:00:00

abstract：:Two members of the heat shock transcription factor (HSF) family, HSF1 and HSF2, both function as transcriptional activators of heat shock gene expression. However, the inducible DNA-binding activities of these two factors are regulated by distinct pathways. HSF1 is activated by heat shock and other forms of stress, wh...

journal_title：Molecular and cellular biology

authors： Sistonen L,Sarge KD,Morimoto RI

更新日期：1994-03-01 00:00:00

abstract：:Telomerase is a ribonucleoprotein reverse transcriptase responsible for the maintenance of one strand of the telomere terminal repeats. The catalytic protein subunit of the telomerase complex, known as TERT, possesses a reverse transcriptase (RT) domain that mediates nucleotide addition. The RT domain of TERT is disti...

journal_title：Molecular and cellular biology

authors： Lue NF,Lin YC,Mian IS

更新日期：2003-12-01 00:00:00

abstract：:The MyoD gene can orchestrate the expression of the skeletal muscle differentiation program. We have identified the regions of the gene necessary to reproduce transcription specific to skeletal myoblasts and myotubes. A proximal regulatory region (PRR) contains a conserved TATA box, a CCAAT box, and a GC-rich region t...

journal_title：Molecular and cellular biology

authors： Tapscott SJ,Lassar AB,Weintraub H

更新日期：1992-11-01 00:00:00

abstract：:NF-κB activation is a critical component in the transcriptional response to hypoxia. However, the underlying mechanisms that control its activity under these conditions are unknown. Here we report that under hypoxic conditions, IκB kinase (IKK) activity is induced through a calcium/calmodulin-dependent kinase 2 (CaMK2...

journal_title：Molecular and cellular biology

authors： Culver C,Sundqvist A,Mudie S,Melvin A,Xirodimas D,Rocha S

更新日期：2010-10-01 00:00:00

abstract：:In self-renewing tissues such as the skin epidermis and the bone marrow, Myc proteins control differentiation of stem cells and proliferation of progenitor cell types. In the epithelium of the small intestine, we show that c-Myc and N-Myc are expressed in a differential manner. Whereas c-Myc is expressed in the prolif...

journal_title：Molecular and cellular biology

authors： Bettess MD,Dubois N,Murphy MJ,Dubey C,Roger C,Robine S,Trumpp A

更新日期：2005-09-01 00:00:00

abstract：:Using an expression cDNA cloning approach, we examined human tumor cell lines for novel oncogenes that might evade detection by conventional techniques. We isolated a transforming sequence that was highly efficient in transforming NIH 3T3 mouse fibroblasts. DNA sequence analysis identified the gene as the human homolo...

journal_title：Molecular and cellular biology

authors： Chan AM,Fleming TP,McGovern ES,Chedid M,Miki T,Aaronson SA

更新日期：1993-02-01 00:00:00

abstract：:Tetrahymena ciliary membrane vesicles are shown to interact with preconjugant cells in a mating type-specific way. When cells are treated with vesicles of a different mating type before mixing for conjugation, cell pairing is enhanced, and the normal prepairing period is partially eliminated. This enhancement is matin...

journal_title：Molecular and cellular biology

authors： Love B,Rotheim MB

更新日期：1984-04-01 00:00:00

abstract：:SWI-SNF alters DNA-histone interactions within a nucleosome in an ATP-dependent manner. These alterations cause changes in the topology of a closed circular nucleosomal array that persist after removal of ATP from the reaction. We demonstrate here that a remodeled closed circular array will revert toward its original ...

journal_title：Molecular and cellular biology

authors： Guyon JR,Narlikar GJ,Sullivan EK,Kingston RE

更新日期：2001-02-01 00:00:00

abstract：:Using nuclear runoff transcription assays we demonstrated that alpha interferon-mediated induction of transcription of four mRNAs in HeLa monolayer cells needed ongoing protein synthesis and that such a need could be obviated by pretreating the cells with gamma interferon which, by itself, did not induce transcription...

journal_title：Molecular and cellular biology

authors： Kusari J,Sen GC

更新日期：1987-01-01 00:00:00

abstract：:A mutant of Saccharomyces cerevisiae defective in the S-adenosylmethionine (AdoMet)-dependent methyltransferase step of diphthamide biosynthesis was selected by intracellular expression of the F2 fragment of diphtheria toxin (DT) and shown to belong to complementation group DPH5. The DPH5 gene was cloned, sequenced, a...

journal_title：Molecular and cellular biology

authors： Mattheakis LC,Shen WH,Collier RJ

更新日期：1992-09-01 00:00:00

abstract：:As shown by Southern blot analysis, the metallothionein-1 (MT-1) genes in rats comprise a multigene family. We present the sequence of the MT-1 structural gene and compare its features with other metallothionein genes. Three MT-1 pseudogenes which we sequenced apparently arose by reverse transcription of processed mRN...

journal_title：Molecular and cellular biology

authors： Andersen RD,Birren BW,Taplitz SJ,Herschman HR

更新日期：1986-01-01 00:00:00

abstract：:The GTPase-activating protein (GAP) stimulates the GTPase reaction of p21 by 5 orders of magnitude such that the kcat of the reaction is increased to 19 s-1. Mutations of residues in loop L1 (Gly-12 and Gly-13), in loop L2 (Thr-35 and Asp-38), and in loop L4 (Gln-61 and Glu-63) influence the reaction in different ways...

journal_title：Molecular and cellular biology

authors： Gideon P,John J,Frech M,Lautwein A,Clark R,Scheffler JE,Wittinghofer A

更新日期：1992-05-01 00:00:00

abstract：:A monoclonal antibody (MAb 30B6) was recently described by Rogalski and Singer (J. Cell Biol. 101:785-801, 1985) which identified an integral membrane glycoprotein of chicken cells that was associated with a wide variety of sites of actin microfilament attachments to membranes. In this report, we present a further cha...

journal_title：Molecular and cellular biology

authors： Maher PA,Singer SJ

更新日期：1988-02-01 00:00:00

abstract：:We find that during embryogenesis the expression of HMGN1, a nuclear protein that binds to nucleosomes and reduces the compaction of the chromatin fiber, is progressively down-regulated throughout the entire embryo, except in committed but continuously renewing cell types, such as the basal layer of the epithelium. In...

journal_title：Molecular and cellular biology

authors： Furusawa T,Lim JH,Catez F,Birger Y,Mackem S,Bustin M

更新日期：2006-01-01 00:00:00

abstract：:Although an F-box protein, Mdm30, is found to regulate ubiquitylation of the Sub2 component of TREX (transcription-export) complex for proteasomal degradation in stimulation of mRNA export, it remains unknown whether such ubiquitin-proteasome system (UPS) regulation of Sub2 occurs cotranscriptionally via its interacti...

journal_title：Molecular and cellular biology

authors： Ferdoush J,Sen R,Durairaj G,Barman P,Kaja A,Guha S,Bhaumik SR

更新日期：2020-03-16 00:00:00

abstract：:Prostaglandins, pleiotropic immune modulators that induce protein kinase A (PKA), inhibit gamma interferon induction of class II major histocompatibility complex (MHC) genes. We show that phosphorylation of CIITA by PKA accounts for this inhibition. Treatment with prostaglandin E or 8-bromo-cyclic AMP or transfection ...

journal_title：Molecular and cellular biology

authors： Li G,Harton JA,Zhu X,Ting JP

更新日期：2001-07-01 00:00:00

abstract：:Selenoprotein mRNAs are potential targets for degradation via nonsense-mediated decay due to the presence of in-frame UGA codons that can be decoded as either selenocysteine or termination codons. When UGA decoding is inefficient, as occurs when selenium is limiting, termination occurs at these positions. Based on the...

journal_title：Molecular and cellular biology

authors： Squires JE,Stoytchev I,Forry EP,Berry MJ

更新日期：2007-11-01 00:00:00

abstract：:Loss of p53 sensitizes to antimicrotubule agents in human tumor cells, but little is known about its role during mitosis. We have identified the Polo-like kinase family member serum inducible kinase (Snk/Plk2) as a novel p53 target gene. Snk/Plk2 mutagenesis demonstrated that its kinase activity is negatively regulate...

journal_title：Molecular and cellular biology

authors： Burns TF,Fei P,Scata KA,Dicker DT,El-Deiry WS

更新日期：2003-08-01 00:00:00

abstract：:We have characterized the putative AP1 site in the backbone of pUC plasmids and found unique regulatory effects. The site, which mapped to a 19-bp region around nucleotide 37, conferred transcriptional activation by Jun or Jun/Fos that was boosted up to fivefold by unliganded thyroid hormone receptor (TR). Thyroid hor...

journal_title：Molecular and cellular biology

authors： Lopez G,Schaufele F,Webb P,Holloway JM,Baxter JD,Kushner PJ

更新日期：1993-05-01 00:00:00