Down-regulation of nucleosomal binding protein HMGN1 expression during embryogenesis modulates Sox9 expression in chondrocytes.

abstract：:Upon the addition of different growth factors and cytokines, the Gab1 docking protein is tyrosine phosphorylated and in turn activates different signaling pathways. On the basis of the large body of evidence concerning cross talk between the signaling pathways activated by growth factors and oxidative stress, we decid...

journal_title：Molecular and cellular biology

authors： Holgado-Madruga M,Wong AJ

更新日期：2003-07-01 00:00:00

abstract：:The class II transactivator (CIITA) is induced by gamma interferon (IFN-gamma) and activates major histocompatibility complex class II; however, this report shows it suppresses other genes. An N-terminal 36 amino acids of CIITA mediates suppression of the collagen alpha(2)(I) promoter via binding to CREB-binding prote...

journal_title：Molecular and cellular biology

authors： Zhu XS,Ting JP

更新日期：2001-10-01 00:00:00

abstract：:Hepatocyte nuclear factor 4α (HNF4α) is a critical factor for hepatocyte differentiation. HNF4α expression is decreased in hepatocellular carcinoma (HCC), which suggests a role in repression of hepatocyte dedifferentiation. In the present study, hepatic expression of HNF4γ was increased in liver-specific Hnf4a-null mi...

journal_title：Molecular and cellular biology

authors： Sasaki S,Urabe M,Maeda T,Suzuki J,Irie R,Suzuki M,Tomaru Y,Sakaguchi M,Gonzalez FJ,Inoue Y

更新日期：2018-11-28 00:00:00

abstract：:Transcription of Xenopus laevis mitochondrial DNA (xl-mtDNA) by the mitochondrial RNA polymerase requires a dissociable factor. This factor was purified to near homogeneity and identified as a 40-kDa protein. A second protein implicated in the transcription of mtDNA, the Xenopus homolog of the HMG box protein mtTFA, w...

journal_title：Molecular and cellular biology

authors： Antoshechkin I,Bogenhagen DF

更新日期：1995-12-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the protein phosphatase type 1 (PP1)-binding protein Reg1 is required to maintain complete repression of ADH2 expression during growth on glucose. Surprisingly, however, mutant forms of the yeast PP1 homologue Glc7, which are unable to repress expression of another glucose-regulated gene, ...

journal_title：Molecular and cellular biology

authors： Dombek KM,Voronkova V,Raney A,Young ET

更新日期：1999-09-01 00:00:00

abstract：:We have recently reported that the yeast chromatin-remodeling factor Swi1 can exist as a prion, [SWI(+)], demonstrating a link between prionogenesis and global transcriptional regulation. To shed light on how the Swi1 conformational switch influences Swi1 function and to define the sequence and structural requirements...

journal_title：Molecular and cellular biology

authors： Du Z,Crow ET,Kang HS,Li L

更新日期：2010-10-01 00:00:00

abstract：:In both tissue sections and cell culture, the endothelial nature of a cell is most commonly determined by demonstration of its expression of von Willebrand factor (vWf) protein and/or mRNA. Thus, the mechanism of cell-type-specific transcriptional regulation of the vWf gene is central to studying the basis of endothel...

journal_title：Molecular and cellular biology

authors： Jahroudi N,Lynch DC

更新日期：1994-02-01 00:00:00

abstract：:A new retrovirus vector containing the gene for hygromycin B resistance (hyg) as a selectable marker under the control of an internal simian virus 40 promoter was constructed. It was used, together with an analogous previously described vector, DO1, which contains the gene for G418 resistance, to introduce and express...

journal_title：Molecular and cellular biology

authors： Yang Z,Korman AJ,Cooper J,Pious D,Accolla RS,Mulligan RC,Strominger JL

更新日期：1987-11-01 00:00:00

abstract：:Methyl-accepting assays and a sensitive method for labeling specific CpG sites have been used to show that the DNA of F9 embryonal carcinoma cells decreases in 5-methylcytosine content by ca. 9% during retinoic acid-induced differentiation, whereas the DNA of dimethyl sulfoxide-induced Friend murine erythroleukemia (M...

journal_title：Molecular and cellular biology

authors： Bestor TH,Hellewell SB,Ingram VM

更新日期：1984-09-01 00:00:00

abstract：:The functional importance of the two clusters of positively charged amino acids which flank the hydrophobic membrane-anchoring sequence of polyomavirus middle T (mT) protein has been investigated by using site-directed mutagenesis. A clear asymmetry was apparent. No effect on transformation was seen following multiple...

journal_title：Molecular and cellular biology

authors： Dahl J,Thathamangalam U,Freund R,Benjamin TL

更新日期：1992-11-01 00:00:00

abstract：:Myocyte enhancer factor 2 (MEF2) transcription factors cooperate with the MyoD family of basic helix-loop-helix (bHLH) transcription factors to drive skeletal muscle development during embryogenesis, but little is known about the potential functions of MEF2 factors in postnatal skeletal muscle. Here we show that skele...

journal_title：Molecular and cellular biology

authors： Potthoff MJ,Arnold MA,McAnally J,Richardson JA,Bassel-Duby R,Olson EN

更新日期：2007-12-01 00:00:00

abstract：:Recently, we demonstrated that the microRNA 424(322)/503 [miR-424(322)/503] cluster is transcriptionally controlled by transforming growth factor β (TGF-β) in the mammary epithelium. Induction of this microRNA cluster impacts mammary epithelium fate by regulating apoptosis and insulin-like growth factor 1 (IGF1) signa...

journal_title：Molecular and cellular biology

authors： Llobet-Navas D,Rodriguez-Barrueco R,de la Iglesia-Vicente J,Olivan M,Castro V,Saucedo-Cuevas L,Marshall N,Putcha P,Castillo-Martin M,Bardot E,Ezhkova E,Iavarone A,Cordon-Cardo C,Silva JM

更新日期：2014-12-01 00:00:00

abstract：:The growth suppressor activities of the RB and p107 products are believed to be mediated by the reversible binding of a heterogeneous family of cellular proteins to a conserved T/E1A pocket domain that is present within both proteins. To study the functional role of these interactions, we examined the properties of ce...

journal_title：Molecular and cellular biology

authors： Kim YW,Otterson GA,Kratzke RA,Coxon AB,Kaye FJ

更新日期：1994-11-01 00:00:00

abstract：:Gradients of Wnt/beta-catenin signaling coordinate development and physiological homeostasis in metazoan animals. Proper embryonic development of the fruit fly Drosophila melanogaster requires the Naked cuticle (Nkd) protein to attenuate a gradient of Wnt/beta-catenin signaling across each segmental anlage. Nkd inhibi...

journal_title：Molecular and cellular biology

authors： Zhang S,Cagatay T,Amanai M,Zhang M,Kline J,Castrillon DH,Ashfaq R,Oz OK,Wharton KA Jr

更新日期：2007-06-01 00:00:00

abstract：:The mechanism(s) and site(s) of action of volatile inhaled anesthetics are unknown in spite of the clinical use of these agents for more than 150 years. In the present study, the model eukaryote Saccharomyces cerevisiae was used to investigate the action of anesthetic agents because of its powerful molecular genetics....

journal_title：Molecular and cellular biology

authors： Keil RL,Wolfe D,Reiner T,Peterson CJ,Riley JL

更新日期：1996-07-01 00:00:00

abstract：:Expression of the avian very-low-density apolipoprotein II (apoVLDLII) gene is completely dependent on estrogen and restricted to the liver. We have identified binding sites for nonhistone nuclear proteins located between -1.96 and -2.61 kilobases. One of these sites, located at -2.6 kilobases (designated site 1), was...

journal_title：Molecular and cellular biology

authors： Hoodless PA,Roy RN,Ryan AK,Haché RJ,Vasa MZ,Deeley RG

更新日期：1990-01-01 00:00:00

abstract：:Amino acids 106 to 143 and 354 to 433 of the human c-myc protein (439 amino acids) were shown to be required for the protein to suppress c-myc gene transcription and were found to exactly overlap with those necessary for c-myc to cooperate with ras oncogenes in the transformation of rat embryo fibroblasts. The essenti...

journal_title：Molecular and cellular biology

authors： Penn LJ,Brooks MW,Laufer EM,Littlewood TD,Morgenstern JP,Evan GI,Lee WM,Land H

更新日期：1990-09-01 00:00:00

abstract：:Rad53 of Saccharomyces cerevisiae is a checkpoint kinase whose structure and function are conserved among eukaryotes. When a cell detects damaged DNA, Rad53 activity is dramatically increased, which ultimately leads to changes in DNA replication, repair, and cell division. Despite its central role in checkpoint signal...

journal_title：Molecular and cellular biology

authors： Sidorova JM,Breeden LL

更新日期：2003-05-01 00:00:00

abstract：:First characterized in Trypanosoma brucei, the spliced leader-associated (SLA) RNA gene locus has now been isolated from the kinetoplastids Leishmania tarentolae and Trypanosoma cruzi. In addition to the T. brucei SLA RNA, both L. tarentolae and T. cruzi SLA RNA repeat units also yield RNAs of 75 or 76 nucleotides (nt...

journal_title：Molecular and cellular biology

authors： Roberts TG,Sturm NR,Yee BK,Yu MC,Hartshorne T,Agabian N,Campbell DA

更新日期：1998-08-01 00:00:00

abstract：:RNA interference (RNAi) in animals, cosuppression in plants, and quelling in fungi are homology-dependent gene silencing mechanisms in which the introduction of either double-stranded RNA (dsRNA) or transgenes induces sequence-specific mRNA degradation. These phenomena share a common genetic and mechanistic basis. The...

journal_title：Molecular and cellular biology

authors： Catalanotto C,Pallotta M,ReFalo P,Sachs MS,Vayssie L,Macino G,Cogoni C

更新日期：2004-03-01 00:00:00

abstract：:The TATA-binding protein (TBP), TFIIA, and TFIIB interact with promoter DNA to form a complex required for transcriptional initiation, and many transcriptional regulators function by either stimulating or inhibiting formation of this complex. We have recently identified TBP mutants that are viable in wild-type cells b...

journal_title：Molecular and cellular biology

authors： Biswas D,Imbalzano AN,Eriksson P,Yu Y,Stillman DJ

更新日期：2004-09-01 00:00:00

abstract：:Using nuclear runoff transcription assays we demonstrated that alpha interferon-mediated induction of transcription of four mRNAs in HeLa monolayer cells needed ongoing protein synthesis and that such a need could be obviated by pretreating the cells with gamma interferon which, by itself, did not induce transcription...

journal_title：Molecular and cellular biology

authors： Kusari J,Sen GC

更新日期：1987-01-01 00:00:00

abstract：:Circadian clock genes are regulated through a transcriptional-translational feedback loop. Alterations of the chromatin structure by histone acetyltransferases and histone deacetylases (HDACs) are commonly implicated in the regulation of gene transcription. However, little is known about the transcriptional regulation...

journal_title：Molecular and cellular biology

authors： Naruse Y,Oh-hashi K,Iijima N,Naruse M,Yoshioka H,Tanaka M

更新日期：2004-07-01 00:00:00

abstract：:We have partially purified a nuclear protein (PPT) from Physarum polycephalum that binds to the extrachromosomal ribosomal DNA telomeres of this acellular slime mold. Binding is specific for the (T2AG3)n telomere repeats, as evidenced by nitrocellulose filter binding assays, by gel mobility shift assays with both DNA ...

journal_title：Molecular and cellular biology

authors： Coren JS,Epstein EM,Vogt VM

更新日期：1991-04-01 00:00:00

abstract：:While early steps of gene expression, such as transcription preinitiation, are known to often be rate limiting and to be regulated by such stimuli as steroid hormones, the potential impact of downstream steps, including splicing, on the mRNA production rate is unknown. In this work, we studied the effects of the trans...

journal_title：Molecular and cellular biology

authors： Bittencourt D,Dutertre M,Sanchez G,Barbier J,Gratadou L,Auboeuf D

更新日期：2008-09-01 00:00:00

abstract：:The human neuroblastoma cell line CHP100 provides a useful model system in which to study the molecular mechanisms of transcriptional regulation of the low-affinity nerve growth factor receptor (NGFR) gene during neuronal development. Basic fibroblast growth factor (bFGF) induced morphological changes in CHP100 cells,...

journal_title：Molecular and cellular biology

authors： Taiji M,Taiji K,Deyerle KL,Bothwell M

更新日期：1992-05-01 00:00:00

abstract：:Methylation-controlled J protein (MCJ) is a newly identified member of the DnaJ family of cochaperones. Hypermethylation-mediated transcriptional silencing of the MCJ gene has been associated with increased chemotherapeutic resistance in ovarian cancer. However, the biology and function of MCJ remain unknown. Here we ...

journal_title：Molecular and cellular biology

authors： Hatle KM,Neveu W,Dienz O,Rymarchyk S,Barrantes R,Hale S,Farley N,Lounsbury KM,Bond JP,Taatjes D,Rincón M

更新日期：2007-04-01 00:00:00

abstract：:The adenovirus type 5 early region 1A (E1A) gene was introduced into neu-transformed B104-1-1 cells. Cells that expressed E1A possessed reduced transforming activity in vitro and reduced tumorigenicity in nude mice. These results demonstrate that the E1A gene products can act negatively to suppress the transformed phe...

journal_title：Molecular and cellular biology

authors： Yu DH,Scorsone K,Hung MC

更新日期：1991-03-01 00:00:00

abstract：:In a previous paper, we have shown that in the absence of stress, mouse embryonal carcinoma cells, like mouse early embryo multipotent cells, synthesize high levels of 89- and 70-kilodalton heat shock proteins (HSP)(O. Bensaude and M. Morange, EMBO J. 2:173-177, 1983). We report here the pattern of proteins synthesize...

journal_title：Molecular and cellular biology

authors： Morange M,Diu A,Bensaude O,Babinet C

更新日期：1984-04-01 00:00:00

abstract：:The 2 micron plasmid of Saccharomyces cerevisiae codes for a site-specific recombinase, the FLP protein, that catalyzes efficient recombination across two 599-base-pair (bp) inverted repeats of the plasmid DNA both in vivo and in vitro. We analyzed the interaction of the purified FLP protein with the target sequences ...

journal_title：Molecular and cellular biology

authors： Andrews BJ,McLeod M,Broach J,Sadowski PD

更新日期：1986-07-01 00:00:00