Mitochondrial DNA instability and peri-implantation lethality associated with targeted disruption of nuclear respiratory factor 1 in mice.

abstract：:U6 RNA genes from the trypanosomatids Crithidia fasciculata and Leptomonas seymouri have been isolated and sequenced. As in Trypanosoma brucei, the U6 RNA genes in both C. fasciculata and L. seymouri are arranged in close linkage with upstream tRNA genes. The U6 RNA sequences from C. fasciculata and L. seymouri deviat...

journal_title：Molecular and cellular biology

authors： Xu GL,Wieland B,Bindereif A

更新日期：1994-07-01 00:00:00

abstract：:The tetradecanoyl phorbol acetate-induced sequence 7 gene (tis7) is regulated during cell fate processes and functions as a transcriptional coregulator. Here, we describe the generation and analysis of mice lacking the tis7 gene. Surprisingly, TIS7 knockout mice show no gross histological abnormalities and are fertile...

journal_title：Molecular and cellular biology

authors： Vadivelu SK,Kurzbauer R,Dieplinger B,Zweyer M,Schafer R,Wernig A,Vietor I,Huber LA

更新日期：2004-04-01 00:00:00

abstract：:The myeloperoxidase (MPO) gene is transcribed specifically in immature myeloid cells and is regulated in part by a 414-bp proximal enhancer. Mutation of a core binding factor (CBF)-binding site at -288 decreased enhancer activity 30-fold in 32D cl3 myeloid cells cultured in granulocyte colony-stimulating factor (G-CSF...

journal_title：Molecular and cellular biology

authors： Britos-Bray M,Friedman AD

更新日期：1997-09-01 00:00:00

abstract：:Drosophila sine oculis and eyes absent genes synergize in compound-eye formation. The murine homologues of these genes, Six and Eya, respectively, show overlapping expression patterns during development. We hypothesized that Six and Eya proteins cooperate to regulate their target genes. Cotransfection assays were perf...

journal_title：Molecular and cellular biology

authors： Ohto H,Kamada S,Tago K,Tominaga SI,Ozaki H,Sato S,Kawakami K

更新日期：1999-10-01 00:00:00

abstract：:Three DNA polymerases, polymerases α, δ, and ε (Pol α, Pol δ, and Pol ε), are responsible for eukaryotic genome duplication. When DNA replication stress is encountered, DNA synthesis stalls until the stress is ameliorated. However, it is not known whether there is a difference in the association of each polymerase wit...

journal_title：Molecular and cellular biology

authors： Yu C,Gan H,Zhang Z

更新日期：2017-10-13 00:00:00

abstract：:G proteins are molecular switches that control a wide variety of physiological functions, including neurotransmission, transcriptional activation, cell migration, cell growth. and proliferation. The ability of GTPases to participate in signaling events is determined by the ratio of GTP-bound to GDP-bound forms in the ...

journal_title：Molecular and cellular biology

authors： Xia C,Ma W,Stafford LJ,Liu C,Gong L,Martin JF,Liu M

更新日期：2003-04-01 00:00:00

abstract：:Tumor progression locus 2 (TPL-2) functions as a MEK-1/2 kinase, which is essential for Toll-like receptor 4 (TLR4) activation of extracellular signal-regulated kinase 1 and 2 (ERK-1/2) mitogen-activated protein (MAP) kinases in lipopolysaccharide (LPS)-stimulated macrophages and for inducing the production of the pro...

journal_title：Molecular and cellular biology

authors： Roget K,Ben-Addi A,Mambole-Dema A,Gantke T,Yang HT,Janzen J,Morrice N,Abbott D,Ley SC

更新日期：2012-11-01 00:00:00

abstract：:The Csk tyrosine kinase negatively regulates the Src family kinases Lck and Fyn in T cells. Engagement of the T-cell antigen receptor results in a removal of Csk from the lipid raft-associated transmembrane protein PAG/Cbp. Instead, Csk becomes associated with an approximately 72-kDa tyrosine-phosphorylated protein, w...

journal_title：Molecular and cellular biology

authors： Rahmouni S,Vang T,Alonso A,Williams S,van Stipdonk M,Soncini C,Moutschen M,Schoenberger SP,Mustelin T

更新日期：2005-03-01 00:00:00

abstract：:Transcription of the Drosophila homologs of the abl and src cellular oncogenes, termed Dash and Dsrc , respectively, was studied. Despite the fact that Dash and Dsrc share sequence homology, they have distinct patterns of transcription during development. The Dash transcript, 6.2 kilobases long, is found in maternal R...

journal_title：Molecular and cellular biology

authors： Lev Z,Leibovitz N,Segev O,Shilo BZ

更新日期：1984-05-01 00:00:00

abstract：:A novel pp90rsk Ser/Thr kinase (referred to as RSK3) was cloned from a human cDNA library. The RSK3 cDNA encodes a predicted 733-amino-acid protein with a unique N-terminal region containing a putative nuclear localization signal. RSK3 mRNA was widely expressed (but was predominant in lung and skeletal muscle). By usi...

journal_title：Molecular and cellular biology

authors： Zhao Y,Bjørbaek C,Weremowicz S,Morton CC,Moller DE

更新日期：1995-08-01 00:00:00

abstract：:We have studied the abilities of different transactivation domains to stimulate the initiation and elongation (postinitiation) steps of RNA polymerase II transcription in vivo. Nuclear run-on and RNase protection analyses revealed three classes of activation domains: Sp1 and CTF stimulated initiation (type I); human i...

journal_title：Molecular and cellular biology

authors： Blau J,Xiao H,McCracken S,O'Hare P,Greenblatt J,Bentley D

更新日期：1996-05-01 00:00:00

abstract：:Axin is a recently identified protein encoded by the fused locus in mice that is required for normal vertebrate axis formation. We have defined a 25-amino-acid sequence in axin that comprises the glycogen synthase kinase 3beta (GSK-3beta) interaction domain (GID). In contrast to full-length axin, which has been shown ...

journal_title：Molecular and cellular biology

authors： Hedgepeth CM,Deardorff MA,Rankin K,Klein PS

更新日期：1999-10-01 00:00:00

abstract：:Nutritional excess and/or obesity represent well-known predisposition factors for the development of non-insulin-dependent diabetes mellitus (NIDDM). However, molecular links between obesity and NIDDM are only beginning to emerge. Here, we demonstrate that nutrients suppress phosphatidylinositol 3 (PI3)-kinase/Akt sig...

journal_title：Molecular and cellular biology

authors： Tzatsos A,Kandror KV

更新日期：2006-01-01 00:00:00

abstract：:We sought to study the binding constraints placed on the nine-zinc-finger protein transcription factor IIIA (TFIIIA) by a histone octamer. To this end, five overlapping fragments of the Xenopus laevis oocyte and somatic 5S rRNA genes were reconstituted into nucleosomes, and it was subsequently shown that nucleosome tr...

journal_title：Molecular and cellular biology

authors： Howe L,Ausió J

更新日期：1998-03-01 00:00:00

abstract：:The human and murine MOK2 ortholog genes that are preferentially expressed in brain and testis tissues encode two different Krüppel-like zinc finger proteins. In this paper, we show that the MOK2 proteins are mainly associated with nuclear ribonucleoprotein components, including the nucleoli and extranucleolar structu...

journal_title：Molecular and cellular biology

authors： Arranz V,Harper F,Florentin Y,Puvion E,Kress M,Ernoult-Lange M

更新日期：1997-04-01 00:00:00

abstract：:TFIID, comprising the TATA box binding protein (TBP) and 13 TBP-associated factors (TAFs), plays a role in nucleation in the assembly of the RNA polymerase II preinitiation complexes on protein-encoding genes. TAFs are shared among other transcription regulatory complexes (e.g., SAGA, TBP-free TAF-containing complex [...

journal_title：Molecular and cellular biology

authors： Soutoglou E,Demény MA,Scheer E,Fienga G,Sassone-Corsi P,Tora L

更新日期：2005-05-01 00:00:00

abstract：:Chicken heart mesenchymal cells do not proliferate in medium of physiological composition containing plasma (S. Balk, Proc. Natl. Acad. Sci. USA 77:6606-6610, 1980). To understand the molecular events involved in cell quiescence and in the initiation of cell division under physiological conditions, we examined the dif...

journal_title：Molecular and cellular biology

authors： Bédard PA,Balk SD,Gunther HS,Morisi A,Erikson RL

更新日期：1987-04-01 00:00:00

abstract：:The formation of inosine at the wobble position of eukaryotic tRNAs is an essential modification catalyzed by the ADAT2/ADAT3 complex. In humans, a valine-to-methionine mutation (V144M) in ADAT3 that originated ∼1,600 years ago is the most common cause of autosomal recessive intellectual disability (ID) in Arabia. Whi...

journal_title：Molecular and cellular biology

authors： Ramos J,Han L,Li Y,Hagelskamp F,Kellner SM,Alkuraya FS,Phizicky EM,Fu D

更新日期：2019-09-11 00:00:00

abstract：:Mutant strain d48 of Paramecium tetraurelia lacks the gene for antigen A in the macronucleus, whereas this gene is present in the micronucleus. Transfer of macronucleoplasm from the wild type to strain d48 caused d48 to revert to the wild type after autogamy. Transfer of cytoplasm was not as effective as transfer of m...

journal_title：Molecular and cellular biology

authors： Harumoto T

更新日期：1986-10-01 00:00:00

abstract：:We describe the cloning and characterization of tumor necrosis factor receptor (TNF-R)-associated ubiquitous scaffolding and signaling protein (TRUSS), a novel TNF-R1-interacting protein of 90.7 kDa. TRUSS mRNA was ubiquitously expressed in mouse tissues but was enriched in heart, liver, and testis. Co-immunoprecipita...

journal_title：Molecular and cellular biology

authors： Soond SM,Terry JL,Colbert JD,Riches DW

更新日期：2003-11-01 00:00:00

abstract：:The CACCC-box binding protein erythroid Krüppel-like factor (EKLF/KLF1) is a master regulator that directs the expression of many important erythroid genes. We have previously shown that EKLF drives transcription of the gene for a second KLF, basic Krüppel-like factor, or KLF3. We have now tested the in vivo role of K...

journal_title：Molecular and cellular biology

authors： Funnell AP,Norton LJ,Mak KS,Burdach J,Artuz CM,Twine NA,Wilkins MR,Power CA,Hung TT,Perdomo J,Koh P,Bell-Anderson KS,Orkin SH,Fraser ST,Perkins AC,Pearson RC,Crossley M

更新日期：2012-08-01 00:00:00

abstract：:The connections between various nuclear processes and specific histone posttranslational modifications are dependent to a large extent on the acquisition of those modifications after histone synthesis. The reestablishment of histone posttranslational modifications after S phase is especially critical for H3K9 and H3K2...

journal_title：Molecular and cellular biology

authors： Zee BM,Britton LM,Wolle D,Haberman DM,Garcia BA

更新日期：2012-07-01 00:00:00

abstract：:The phosphoinositide phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] regulates the activity of many actin-binding proteins and as such is an important modulator of cytoskeleton organization during cell migration, for example. In migrating cells actin remodeling is tightly regulated and localized; therefore, how the ...

journal_title：Molecular and cellular biology

authors： Kisseleva M,Feng Y,Ward M,Song C,Anderson RA,Longmore GD

更新日期：2005-05-01 00:00:00

abstract：:The human glycoprotein IIB (GPIIB) gene is expressed only in megakaryocytes, and its promoter displays cell type specificity. We show that this specificity involved two cis-acting sequences. The first one, located at -55, contains a GATA binding site. Point mutations that abolish protein binding on this site decrease ...

journal_title：Molecular and cellular biology

authors： Lemarchandel V,Ghysdael J,Mignotte V,Rahuel C,Roméo PH

更新日期：1993-01-01 00:00:00

abstract：:Double-stranded RNA (dsRNA) accumulates in virus-infected mammalian cells and signals the activation of host defense pathways of the interferon system. We describe here a novel form of dsRNA-triggered signaling that leads to the stimulation of the p38 mitogen-activated protein kinase (p38 MAPK) and the c-Jun NH(2)-ter...

journal_title：Molecular and cellular biology

authors： Iordanov MS,Paranjape JM,Zhou A,Wong J,Williams BR,Meurs EF,Silverman RH,Magun BE

更新日期：2000-01-01 00:00:00

abstract：:We have examined the ability of the simian virus 40 72-base pair enhancer segment to simultaneously activate multiple transcription units with plasmids that contain one, two, or three simian virus 40-based transcription units in various arrangements. After transfection into CV1 cells, the expression of a marker gene, ...

journal_title：Molecular and cellular biology

authors： Kadesch T,Berg P

更新日期：1986-07-01 00:00:00

abstract：:The levels of the A, B, and C subunits of protein phosphatase 2A in extracts from synchronized embryonic bovine tracheal cells were determined by immunoblotting with subunit-specific antibodies. A constant amount of each subunit was found in resting cells as well as in growing cells from all stages of the cell cycle. ...

journal_title：Molecular and cellular biology

authors： Ruediger R,Van Wart Hood JE,Mumby M,Walter G

更新日期：1991-08-01 00:00:00

abstract：:Irradiation of simian virus 40 (SV40)-infected cells with low fluences of UV light (20 to 60 J/m2, inducing one to three pyrimidine dimers per SV40 genome) causes a dramatic inhibition of viral DNA replication. However, treatment of cells with UV radiation (20 J/m2) before infection with SV40 virus enhances the replic...

journal_title：Molecular and cellular biology

authors： Treger JM,Hauser J,Dixon K

更新日期：1988-06-01 00:00:00

abstract：:The CYC1-239-O mutation in the yeast Saccharomyces cerevisiae produces a -His-Leu- replacement of the normal -Ala-Gly- sequence at amino acid positions 5 and 6, which lie within a dispensable region of iso-1-cytochrome c; this mutation can accommodate the formation of a hairpin structure at the corresponding site in t...

journal_title：Molecular and cellular biology

authors： Baim SB,Pietras DF,Eustice DC,Sherman F

更新日期：1985-08-01 00:00:00

abstract：:The C-terminal domain of the RNA polymerase II largest subunit (the Rpb1 CTD) is composed of tandem heptad repeats of the consensus sequence Y(1)S(2)P(3)T(4)S(5)P(6)S(7). We reported previously that Thr 4 is phosphorylated and functions in histone mRNA 3'-end formation in chicken DT40 cells. Here, we have extended our...

journal_title：Molecular and cellular biology

authors： Hsin JP,Xiang K,Manley JL

更新日期：2014-07-01 00:00:00