Abstract:
:The CYC1-239-O mutation in the yeast Saccharomyces cerevisiae produces a -His-Leu- replacement of the normal -Ala-Gly- sequence at amino acid positions 5 and 6, which lie within a dispensable region of iso-1-cytochrome c; this mutation can accommodate the formation of a hairpin structure at the corresponding site in the mRNA. The amount of the altered protein was diminished to 20% of the wild-type level, whereas the amount of the mRNA remained normal. However, in contrast to the normal CYC1+ mRNA that is associated mainly with four to seven ribosomes, the bulk of the CYC1-239-O mRNA is associated with one to four ribosomes. These results suggest that the stable secondary structure within the translated region of the CYC1 mRNA diminishes translation by inhibiting elongation.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Baim SB,Pietras DF,Eustice DC,Sherman Fdoi
10.1128/mcb.5.8.1839subject
Has Abstractpub_date
1985-08-01 00:00:00pages
1839-46issue
8eissn
0270-7306issn
1098-5549journal_volume
5pub_type
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