Active role for nibrin in the kinetics of atm activation.

abstract：:Cultured animal cells rearrange foreign DNA very efficiently by homologous recombination. The individual steps that constitute the mechanism(s) of homologous recombination in transfected DNA are as yet undefined. In this study, we examined the topological requirements by using the genome of simian virus 40 (SV40) as a...

journal_title：Molecular and cellular biology

authors： Wake CT,Vernaleone F,Wilson JH

更新日期：1985-08-01 00:00:00

abstract：:Topoisomerase I cleavage complexes can be induced by a variety of DNA damages and by the anticancer drug camptothecin. We have developed a ligation-mediated PCR (LM-PCR) assay to analyze replication-mediated DNA double-strand breaks induced by topoisomerase I cleavage complexes in human colon carcinoma HT29 cells at t...

journal_title：Molecular and cellular biology

authors： Strumberg D,Pilon AA,Smith M,Hickey R,Malkas L,Pommier Y

更新日期：2000-06-01 00:00:00

abstract：:We previously observed that nitric oxide (NO) exposure increases the stability of mRNAs encoding heme oxygenase 1 (HO-1) and TIEG-1 in human and mouse fibroblasts. Here, we have used microarrays to look broadly for changes in mRNA stability in response to NO treatment. Using human IMR-90 and mouse NIH 3T3 fibroblasts ...

journal_title：Molecular and cellular biology

authors： Kuwano Y,Rabinovic A,Srikantan S,Gorospe M,Demple B

更新日期：2009-05-01 00:00:00

abstract：:The signaling routes linking G-protein-coupled receptors to mitogen-activated protein kinase (MAPK) may involve tyrosine kinases, phosphoinositide 3-kinase gamma (PI3Kgamma), and protein kinase C (PKC). To characterize the mitogenic pathway of bradykinin (BK), COS-7 cells were transiently cotransfected with the human ...

journal_title：Molecular and cellular biology

authors： Adomeit A,Graness A,Gross S,Seedorf K,Wetzker R,Liebmann C

更新日期：1999-08-01 00:00:00

abstract：:Individual steps in the processing of pre-mRNA, including 5'-end cap formation, splicing, and 3'-end processing (cleavage and polyadenylation) are highly integrated and can influence one another. In addition, prior splicing can influence downstream steps in gene expression, including export of mRNA from the nucleus. H...

journal_title：Molecular and cellular biology

authors： McCracken S,Lambermon M,Blencowe BJ

更新日期：2002-01-01 00:00:00

abstract：:We employed a protein gel blotting procedure to search for nuclear proteins from rat pituitary cells that bind preferentially to the 5'-flanking region of the rat prolactin gene. By gel blots of chromatin proteins from GH3 rat pituitary tumor cells with a 32P-labeled prolactin genomic clone, we detected two major bind...

journal_title：Molecular and cellular biology

authors： White BA,Preston GM,Lufkin TC,Bancroft C

更新日期：1985-11-01 00:00:00

abstract：:The gene encoding human hemojuvelin (HJV) is one of the genes that, when mutated, can cause juvenile hemochromatosis, an early-onset inherited disorder associated with iron overload. The 5' untranslated region of the human HJV mRNA has two upstream open reading frames (uORFs), with 28 and 19 codons formed by two upstr...

journal_title：Molecular and cellular biology

authors： Onofre C,Tomé F,Barbosa C,Silva AL,Romão L

更新日期：2015-04-01 00:00:00

abstract：:The high-affinity interleukin 2 receptor (IL-2R) consists of at least three distinct subunits: the IL-2R alpha chain (IL-2R alpha), beta chain (IL-2R beta), and gamma chain (IL-2R gamma). It has been shown that the cytoplasmic region of IL-2R beta, but not of IL-2R alpha, is essential for IL-2 signalling to the cell i...

journal_title：Molecular and cellular biology

authors： Kawahara A,Minami Y,Taniguchi T

更新日期：1994-08-01 00:00:00

abstract：:Inactivation of gene expression by antisense mechanisms in general and by ribozymes in particular is a powerful technique for studying the function of a gene product. We have designed a strategy for expression of ribozymes, for selection of accessible cleavage sites in target RNAs, and for isolation of ribozymes from ...

journal_title：Molecular and cellular biology

authors： Lieber A,Strauss M

更新日期：1995-01-01 00:00:00

abstract：:GCN5, a putative transcriptional adapter in humans and yeast, possesses histone acetyltransferase (HAT) activity which has been linked to GCN5's role in transcriptional activation in yeast. In this report, we demonstrate a functional interaction between human GCN5 (hGCN5) and the DNA-dependent protein kinase (DNA-PK) ...

journal_title：Molecular and cellular biology

authors： Barlev NA,Poltoratsky V,Owen-Hughes T,Ying C,Liu L,Workman JL,Berger SL

更新日期：1998-03-01 00:00:00

abstract：:Sphingomyelin is a major lipid in the bilayer of subcellular membranes of eukaryotic cells. Different sphingomyelinases catalyze the initial step in the catabolism of sphingomyelin, the hydrolysis to phosphocholine and ceramide. Sphingomyelinases have been postulated to generate ceramide as a lipophilic second messeng...

journal_title：Molecular and cellular biology

authors： Zumbansen M,Stoffel W

更新日期：2002-06-01 00:00:00

abstract：:In Saccharomyces cerevisiae, pseudouridine formation in the middle position of the tRNA(Tyr) anticodon (psi 35) is dependent on the presence of the intron in the tRNA(Tyr) gene (Johnson and Abelson, Nature 302:681-687, 1983). Drosophila melanogaster tRNA(Tyr) genes contain introns of three size classes: 20 or 21 base ...

journal_title：Molecular and cellular biology

authors： Choffat Y,Suter B,Behra R,Kubli E

更新日期：1988-08-01 00:00:00

abstract：:By injecting the appropriately constructed plasmids into one-cell Xenopus embryos, we determined that the 3' region of the maternal Xenopus Eg2 mRNA confers instability on the chimeric mRNA transcribed from these plasmids. This instability, like that of the maternal Eg2 transcript, was abolished by treatment of the em...

journal_title：Molecular and cellular biology

authors： Bouvet P,Paris J,Phillippe M,Osborne HB

更新日期：1991-06-01 00:00:00

abstract：:The TIP60 histone acetyltransferase plays diverse roles in DNA damage responses, DNA double-strand break repair, and transcriptional regulation. TIP60 resides within a multisubunit complex that has been shown to be targeted by transcription factors and to be involved in histone acetylation and transcriptional activati...

journal_title：Molecular and cellular biology

authors： Park JH,Sun XJ,Roeder RG

更新日期：2010-06-01 00:00:00

abstract：:Ribosomal DNA (rDNA) genes in eukaryotes are organized into multicopy tandem arrays and transcribed by RNA polymerase I. During cell proliferation, ∼50% of these genes are active and have a relatively open chromatin structure characterized by elevated accessibility to psoralen cross-linking. In Saccharomyces cerevisia...

journal_title：Molecular and cellular biology

authors： Johnson JM,French SL,Osheim YN,Li M,Hall L,Beyer AL,Smith JS

更新日期：2013-07-01 00:00:00

abstract：:TFIID is a multiprotein complex consisting of the TATA box binding protein and multiple tightly associated proteins (TAFIIs) that are required for transcription by selected activators. We previously reported cloning and partial characterization of human TAFII130 (hTAFII130). The central domain of hTAFII130 contains fo...

journal_title：Molecular and cellular biology

authors： Saluja D,Vassallo MF,Tanese N

更新日期：1998-10-01 00:00:00

abstract：:In both tissue sections and cell culture, the endothelial nature of a cell is most commonly determined by demonstration of its expression of von Willebrand factor (vWf) protein and/or mRNA. Thus, the mechanism of cell-type-specific transcriptional regulation of the vWf gene is central to studying the basis of endothel...

journal_title：Molecular and cellular biology

authors： Jahroudi N,Lynch DC

更新日期：1994-02-01 00:00:00

abstract：:Serum induction of c-jun expression in HeLa cells requires a MEF2 site at -59 in the c-jun promoter. MEF2 sites, found in many muscle-specific enhancers, are bound by a family of transcription factors, MEF2A through -D, which are related to serum response factor in their DNA binding domains. We have found that MEF2D i...

journal_title：Molecular and cellular biology

authors： Han TH,Prywes R

更新日期：1995-06-01 00:00:00

abstract：:In Blastocladiella emersonii zoospores, a set of proteins was found associated with the ribosomes and free ribonucleoprotein particles distinct from the ribosomes and polyribosomes. These proteins were designated P120, P105, P64, P56, and P42 based on their molecular weights determined by gel electrophoresis. Synthesi...

journal_title：Molecular and cellular biology

authors： Jaworski AJ,Stumhofer P

更新日期：1981-04-01 00:00:00

abstract：:Cellular levels of diadenosine tetraphosphate (Ap4A) and adenosine tetraphospho-guanosine (Ap4G) were specifically measured during the cell cycle of Physarum polycephalum by a high-pressure liquid chromatographic method. Ap4A was also measured indirectly by a coupled phosphodiesterase-luciferase assay. No cell cycle-s...

journal_title：Molecular and cellular biology

authors： Garrison PN,Mathis SA,Barnes LD

更新日期：1986-04-01 00:00:00

abstract：:Ubiquitin-mediated proteolysis controls diverse physiological processes in eukaryotes. However, few in vivo targets of the mammalian Cdc34 and Rad6 ubiquitin-conjugating enzymes are known. A yeast-based genetic assay to identify proteins that interact with human Cdc34 resulted in three cDNAs encoding bZIP DNA binding ...

journal_title：Molecular and cellular biology

authors： Pati D,Meistrich ML,Plon SE

更新日期：1999-07-01 00:00:00

abstract：:The male-specific-lethal (MSL) proteins in Drosophila melanogaster serve to adjust gene expression levels in male flies containing a single X chromosome to equal those in females with a double dose of X-linked genes. Together with noncoding roX RNA, MSL proteins form the "dosage compensation complex" (DCC), which inte...

journal_title：Molecular and cellular biology

authors： Morales V,Regnard C,Izzo A,Vetter I,Becker PB

更新日期：2005-07-01 00:00:00

abstract：:Polarized cell movement is an essential requisite for cancer metastasis; thus, interference with the tumor cell motility machinery would significantly modify its metastatic behavior. Protein kinase C alpha (PKC alpha) has been implicated in the promotion of a migratory cell phenotype. We report that the phorbol ester-...

journal_title：Molecular and cellular biology

authors： Parsons M,Keppler MD,Kline A,Messent A,Humphries MJ,Gilchrist R,Hart IR,Quittau-Prevostel C,Hughes WE,Parker PJ,Ng T

更新日期：2002-08-01 00:00:00

abstract：:The Saccharomyces cerevisiae CDC25 gene and closely homologous genes in other eukaryotes encode guanine nucleotide exchange factors for Ras proteins. We have determined the minimal region of the budding yeast CDC25 gene capable of activity in vivo. The region required for full biological activity is approximately 450 ...

journal_title：Molecular and cellular biology

authors： Lai CC,Boguski M,Broek D,Powers S

更新日期：1993-03-01 00:00:00

abstract：:p53 promotes apoptosis in response to death stimuli by transactivation of target genes and by transcription-independent mechanisms. We recently showed that wild-type p53 rapidly translocates to mitochondria in response to multiple death stimuli in cultured cells. Mitochondrial p53 physically interacts with antiapoptot...

journal_title：Molecular and cellular biology

authors： Erster S,Mihara M,Kim RH,Petrenko O,Moll UM

更新日期：2004-08-01 00:00:00

abstract：:The RNA binding protein CPEB (cytoplasmic polyadenylation element binding) regulates cytoplasmic polyadenylation and translation in germ cells and the brain. In neurons, CPEB is detected at postsynaptic sites, as well as in the cell body. The related CPEB3 protein also regulates translation in neurons, albeit probably...

journal_title：Molecular and cellular biology

authors： Kan MC,Oruganty-Das A,Cooper-Morgan A,Jin G,Swanger SA,Bassell GJ,Florman H,van Leyen K,Richter JD

更新日期：2010-12-01 00:00:00

abstract：:Interferon (IFN) regulatory factor 1 (IRF-1) and IRF-2 were originally identified as transcription factors involved in the regulation of the IFN system. IRF-1 functions as a transcriptional activator, while IRF-2 represses IRF-1 function. More recently, evidence has been provided that IRF-1 and IRF-2 manifest antionco...

journal_title：Molecular and cellular biology

authors： Tanaka N,Kawakami T,Taniguchi T

更新日期：1993-08-01 00:00:00

abstract：:U6 is the most conserved of the five small nuclear RNAs known to participate in pre-mRNA splicing. In the fission yeast Schizosaccharomyces pombe, the single-copy gene encoding this RNA is itself interrupted by an intron (T. Tani and Y. Ohshima, Nature (London) 337:87-90, 1989). Here we report analysis of the U6 genes...

journal_title：Molecular and cellular biology

authors： Reich C,Wise JA

更新日期：1990-10-01 00:00:00

abstract：:Previous work had identified a corepressor, NAB1, which represses transcriptional activation mediated by NGFI-A (also known as Egr-1, zif268, and Krox24) and Krox20. These zinc finger transcription factors are encoded by immediate-early genes and have been implicated in a wide variety of proliferative and differentiat...

journal_title：Molecular and cellular biology

authors： Svaren J,Sevetson BR,Apel ED,Zimonjic DB,Popescu NC,Milbrandt J

更新日期：1996-07-01 00:00:00

abstract：:The Ki-67 protein is widely used as a tumor proliferation marker. However, whether Ki-67 affects cell cycle progression has been controversial. Here we demonstrate that depletion of Ki-67 in human hTERT-RPE1, WI-38, IMR90, and hTERT-BJ cell lines and primary fibroblast cells slowed entry into S phase and coordinately ...

journal_title：Molecular and cellular biology

authors： Sun X,Bizhanova A,Matheson TD,Yu J,Zhu LJ,Kaufman PD

更新日期：2017-08-11 00:00:00