Conversion of topoisomerase I cleavage complexes on the leading strand of ribosomal DNA into 5'-phosphorylated DNA double-strand breaks by replication runoff.

abstract：:The SNF3 gene of Saccharomyces cerevisiae encodes a high-affinity glucose transporter that is homologous to mammalian glucose transporters. Point mutations affecting the function of the transporter were recovered from the genomes of four snf3 mutants and characterized. Two of the mutations introduced a charged amino a...

journal_title：Molecular and cellular biology

authors： Marshall-Carlson L,Celenza JL,Laurent BC,Carlson M

更新日期：1990-03-01 00:00:00

abstract：:Mutations in the spin gene are characterized by an extraordinarily strong rejection behavior of female flies in response to male courtship. They are also accompanied by decreases in the viability, adult life span, and oviposition rate of the flies. In spin mutants, some oocytes and adult neural cells undergo degenerat...

journal_title：Molecular and cellular biology

authors： Nakano Y,Fujitani K,Kurihara J,Ragan J,Usui-Aoki K,Shimoda L,Lukacsovich T,Suzuki K,Sezaki M,Sano Y,Ueda R,Awano W,Kaneda M,Umeda M,Yamamoto D

更新日期：2001-06-01 00:00:00

abstract：:The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at th...

journal_title：Molecular and cellular biology

authors： Hsu JM,Huang J,Meluh PB,Laurent BC

更新日期：2003-05-01 00:00:00

abstract：:Poly(A)-binding protein (PABP) stimulates translation initiation by binding simultaneously to the mRNA poly(A) tail and eukaryotic translation initiation factor 4G (eIF4G). PABP activity is regulated by PABP-interacting (Paip) proteins. Paip1 binds PABP and stimulates translation by an unknown mechanism. Here, we desc...

journal_title：Molecular and cellular biology

authors： Martineau Y,Derry MC,Wang X,Yanagiya A,Berlanga JJ,Shyu AB,Imataka H,Gehring K,Sonenberg N

更新日期：2008-11-01 00:00:00

abstract：:The functional importance of the two clusters of positively charged amino acids which flank the hydrophobic membrane-anchoring sequence of polyomavirus middle T (mT) protein has been investigated by using site-directed mutagenesis. A clear asymmetry was apparent. No effect on transformation was seen following multiple...

journal_title：Molecular and cellular biology

authors： Dahl J,Thathamangalam U,Freund R,Benjamin TL

更新日期：1992-11-01 00:00:00

abstract：:The simian virus 40 core origin of replication consists of nucleotides 5211 through 31. These 64 base pairs contain three functional domains with strict sequence requirements and two spacer regions with relaxed sequence specificity but precise positional constraints. The early domain extends for 10 contiguous base pai...

journal_title：Molecular and cellular biology

authors： Deb S,DeLucia AL,Baur CP,Koff A,Tegtmeyer P

更新日期：1986-05-01 00:00:00

abstract：:Loss of heterozygosity (LOH) is a common genetic alteration in tumors and often extends several megabases to encompass multiple genetic loci or even whole chromosome arms. Based on marker and karyotype analysis of tumor samples, a significant fraction of LOH events appears to arise from mitotic recombination between h...

journal_title：Molecular and cellular biology

authors： Stark JM,Jasin M

更新日期：2003-01-01 00:00:00

abstract：:Paramecium tetraurelia contains two types of nuclei, a diploid germinal micronucleus and a large transcriptionally active macronucleus. The macronuclear genome is formed from the micronuclear DNA during sexual reproduction. Previous studies have shown that the processing of the A-type variable surface protein gene dur...

journal_title：Molecular and cellular biology

authors： Scott JM,Mikami K,Leeck CL,Forney JD

更新日期：1994-04-01 00:00:00

abstract：:IME1 encodes a transcriptional activator required for the transcription of meiosis-specific genes and initiation of meiosis in Saccharomyces cerevisiae. The transcription of IME1 is repressed in the presence of glucose, and a low basal level of IME1 RNA is observed in vegetative cultures with acetate as the sole carbo...

journal_title：Molecular and cellular biology

authors： Sagee S,Sherman A,Shenhar G,Robzyk K,Ben-Doy N,Simchen G,Kassir Y

更新日期：1998-04-01 00:00:00

abstract：:The polypyrimidine tract near the 3' splice site is important for pre-mRNA splicing. Using pseudouridine incorporation and in vivo RNA-guided RNA pseudouridylation, we have identified two important uridines in the polypyrimidine tract of adenovirus pre-mRNA. Conversion of either uridine into pseudouridine leads to a s...

journal_title：Molecular and cellular biology

authors： Chen C,Zhao X,Kierzek R,Yu YT

更新日期：2010-09-01 00:00:00

abstract：:RBM4 promotes differentiation of neuronal progenitor cells and neurite outgrowth of cultured neurons via its role in splicing regulation. In this study, we further explored the role of RBM4 in neuronal differentiation. During neuronal differentiation, energy production shifts from glycolysis to oxidative phosphorylati...

journal_title：Molecular and cellular biology

authors： Su CH,Hung KY,Hung SC,Tarn WY

更新日期：2017-01-19 00:00:00

abstract：:The assembly of alpha-ketoglutarate dehydrogenase complex (KGDC) has been studied in wild-type Saccharomyces cerevisiae and in respiratory-deficient strains (pet) with mutations in KGD1 and KGD2, the structural genes for alpha-ketoglutarate dehydrogenase (KE1) and dihydrolipoyl transsuccinylase (KE2) components, respe...

journal_title：Molecular and cellular biology

authors： Repetto B,Tzagoloff A

更新日期：1991-08-01 00:00:00

abstract：:Inorganic pyrophosphate promoted the acidification of an intracellular compartment in permeabilized procyclic trypomastigotes of Trypanosoma brucei, as measured by acridine orange uptake. The proton gradient generated by pyrophosphate was collapsed by addition of nigericin or NH(4)Cl. Pyrophosphate-driven proton trans...

journal_title：Molecular and cellular biology

authors： Rodrigues CO,Scott DA,Docampo R

更新日期：1999-11-01 00:00:00

abstract：:A region located at kbp -3.9 to -2.6 5' to the first hematopoietic exon of the GATA-1 gene is necessary to recapitulate gene expression in both the primitive and definitive erythroid lineages. In transfection analyses, this region activated reporter gene expression from an artificial promoter in a position- and orient...

journal_title：Molecular and cellular biology

authors： Nishimura S,Takahashi S,Kuroha T,Suwabe N,Nagasawa T,Trainor C,Yamamoto M

更新日期：2000-01-01 00:00:00

abstract：:The contribution of erythropoietin to the differentiation of the red blood cell lineage remains elusive, and the demonstration of a molecular link between erythropoietin and the transcription of genes associated with erythroid differentiation is lacking. In erythroid cells, expression of the tissue inhibitor of matrix...

journal_title：Molecular and cellular biology

authors： Kadri Z,Maouche-Chretien L,Rooke HM,Orkin SH,Romeo PH,Mayeux P,Leboulch P,Chretien S

更新日期：2005-09-01 00:00:00

abstract：:V(D)J recombination requires binding and synapsis of a complementary (12/23) pair of recombination signal sequences (RSSs) by the RAG1 and RAG2 proteins, aided by a high-mobility group protein, HMG1 or HMG2. Double-strand DNA cleavage within this synaptic, or paired, complex is thought to involve DNA distortion or mel...

journal_title：Molecular and cellular biology

authors： Ciubotaru M,Schatz DG

更新日期：2004-10-01 00:00:00

abstract：:The intergenic spacer region of the Xenopus laevis ribosomal DNA contains multiple elements which are either 60 or 81 base pairs long. Clusters of these elements have previously been shown to act as position- and distance-independent enhancers on an RNA polymerase I promoter when located in cis. By a combination of de...

journal_title：Molecular and cellular biology

authors： Pikaard CS,Reeder RH

更新日期：1988-10-01 00:00:00

abstract：:The immunoglobulin superfamily recognition molecule L1 plays important functional roles in the developing and adult nervous system. Metalloprotease-mediated cleavage of this adhesion molecule has been shown to stimulate cellular migration and neurite outgrowth. We demonstrate here that L1 cleavage is mediated by two d...

journal_title：Molecular and cellular biology

authors： Maretzky T,Schulte M,Ludwig A,Rose-John S,Blobel C,Hartmann D,Altevogt P,Saftig P,Reiss K

更新日期：2005-10-01 00:00:00

abstract：:A special group of mitochondrial outer membrane proteins spans the membrane once, exposing soluble domains to both sides of the membrane. These proteins are synthesized in the cytosol and then inserted into the membrane by an unknown mechanism. To identify proteins that are involved in the biogenesis of the single-spa...

journal_title：Molecular and cellular biology

authors： Papić D,Elbaz-Alon Y,Koerdt SN,Leopold K,Worm D,Jung M,Schuldiner M,Rapaport D

更新日期：2013-10-01 00:00:00

abstract：:Centrins in vertebrates have traditionally been associated with microtubule-nucleating centers such as the centrosome. Unexpectedly, we found centrin 2 to associate biochemically with nucleoporins, including the Xenopus laevis Nup107-160 complex, a critical subunit of the vertebrate nuclear pore in interphase and of t...

journal_title：Molecular and cellular biology

authors： Resendes KK,Rasala BA,Forbes DJ

更新日期：2008-03-01 00:00:00

abstract：:We have combined in vitro DNA replication reactions and immunological techniques to analyze biochemical interactions between simian virus (SV40) large T antigen and components of the cellular replication apparatus. First, in vitro SV40 DNA replication was characterized with specific origin mutants. Next, monoclonal an...

journal_title：Molecular and cellular biology

authors： Smale ST,Tjian R

更新日期：1986-11-01 00:00:00

abstract：:Intron-encoded U17a and U17b RNAs are members of the H/ACA-box class of small nucleolar RNAs (snoRNAs) participating in rRNA processing and modification. We have investigated the organization and expression of the U17 locus in human cells and found that intronic U17a and U17b sequences are transcribed as part of the t...

journal_title：Molecular and cellular biology

authors： Pelczar P,Filipowicz W

更新日期：1998-08-01 00:00:00

abstract：:It has been hypothesized that protein factors may protect CpG islands from methyltransferase during development and that demethylation may involve protein-DNA interactions at demethylated sites. However, direct evidence has been lacking. In this study, demethylation at the EBNA-1 binding sites of the Epstein-Barr viru...

journal_title：Molecular and cellular biology

authors： Hsieh CL

更新日期：1999-01-01 00:00:00

abstract：:Genomic DNA in higher eucaryotic cells is organized into a series of loops, each of which may be affixed at its base to the nuclear matrix via a specific matrix attachment region (MAR). In this report, we describe the distribution of MARs within the amplified dihydrofolate reductase (DHFR) domain (amplicon) in the met...

journal_title：Molecular and cellular biology

authors： Dijkwel PA,Hamlin JL

更新日期：1988-12-01 00:00:00

abstract：:Hypoxia promotes stem cell maintenance and tumor progression, but it remains unclear how it regulates long-term adaptation toward these processes. We reveal a striking downregulation of the hypoxia-inducible histone H3 lysine 9 (H3K9) demethylase JMJD1A as a hallmark of clinical human germ cell-derived tumors, such as...

journal_title：Molecular and cellular biology

authors： Ueda J,Ho JC,Lee KL,Kitajima S,Yang H,Sun W,Fukuhara N,Zaiden N,Chan SL,Tachibana M,Shinkai Y,Kato H,Poellinger L

更新日期：2014-10-01 00:00:00

abstract：:Fibroblasts transformed by Fos oncogenes display increased expression of a number of genes implicated in tumor cell invasion and metastasis. In contrast to normal 208F rat fibroblasts, Fos-transformed 208F fibroblasts are growth factor independent for invasion. We demonstrate that invasion of v-Fos- or epidermal growt...

journal_title：Molecular and cellular biology

authors： Lamb RF,Hennigan RF,Turnbull K,Katsanakis KD,MacKenzie ED,Birnie GD,Ozanne BW

更新日期：1997-02-01 00:00:00

abstract：:Histone acetylation plays an important role in regulating chromatin structure and thus gene expression. Here we describe the functional characterization of HDAC4, a human histone deacetylase whose C-terminal part displays significant sequence similarity to the deacetylase domain of yeast HDA1. HDAC4 is expressed in va...

journal_title：Molecular and cellular biology

authors： Wang AH,Bertos NR,Vezmar M,Pelletier N,Crosato M,Heng HH,Th'ng J,Han J,Yang XJ

更新日期：1999-11-01 00:00:00

abstract：:The growth suppressor activities of the RB and p107 products are believed to be mediated by the reversible binding of a heterogeneous family of cellular proteins to a conserved T/E1A pocket domain that is present within both proteins. To study the functional role of these interactions, we examined the properties of ce...

journal_title：Molecular and cellular biology

authors： Kim YW,Otterson GA,Kratzke RA,Coxon AB,Kaye FJ

更新日期：1994-11-01 00:00:00

abstract：:We previously established that the phage phiC31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors. We show here that phage attP sites inserted at various locations in human and mouse chromosomes serve...

journal_title：Molecular and cellular biology

authors： Thyagarajan B,Olivares EC,Hollis RP,Ginsburg DS,Calos MP

更新日期：2001-06-01 00:00:00

abstract：:A Dictyostelium discoideum cDNA encoding an alpha-type subunit of casein kinase II was isolated, and its cDNA was used to study developmental expression of casein kinase II during the Dictyostelium life cycle. The 1.3-kb cDNA insert contained an open reading frame of 337 amino acids (M(r) 39,900). The deduced amino ac...

journal_title：Molecular and cellular biology

authors： Kikkawa U,Mann SK,Firtel RA,Hunter T

更新日期：1992-12-01 00:00:00