Centrin 2 localizes to the vertebrate nuclear pore and plays a role in mRNA and protein export.

abstract：:We have characterized features of the site recognized by a double-stranded DNA endonuclease, I-SceII, encoded by intron 4 alpha of the yeast mitochondrial COX1 gene. We determined the effects of 36 point mutations on the cleavage efficiency of natural and synthetic substrates containing the Saccharomyces capensis I-Sc...

journal_title：Molecular and cellular biology

authors： Wernette C,Saldanha R,Smith D,Ming D,Perlman PS,Butow RA

更新日期：1992-02-01 00:00:00

abstract：:The Werner syndrome protein (WRN) suppresses the loss of telomeres replicated by lagging-strand synthesis by a yet to be defined mechanism. Here, we show that whereas either WRN or the Bloom syndrome helicase (BLM) stimulates DNA polymerase δ progression across telomeric G-rich repeats, only WRN promotes sequential st...

journal_title：Molecular and cellular biology

authors： Li B,Reddy S,Comai L

更新日期：2017-01-19 00:00:00

abstract：:Mdm2 and MdmX are structurally related p53-binding proteins that function as critical negative regulators of p53 activity in embryonic and adult tissue. The overexpression of Mdm2 or MdmX inhibits p53 tumor suppressor functions in vitro, and the amplification of Mdm2 or MdmX is observed in human cancers retaining wild...

journal_title：Molecular and cellular biology

authors： Matijasevic Z,Steinman HA,Hoover K,Jones SN

更新日期：2008-02-01 00:00:00

abstract：:4.5S RNA is a group of RNAs 90 to 94 nucleotides long (length polymorphism due to a varying number of UMP residues at the 3' end) that form hydrogen bonds with poly(A)-terminated RNAs isolated from mouse, hamster, or rat cells (W. R. Jelinek and L. Leinwand, Cell 15:205-214, 1978; F. Harada, N. Kato, and H.-O. Hoshino...

journal_title：Molecular and cellular biology

authors： Schoeniger LO,Jelinek WR

更新日期：1986-05-01 00:00:00

abstract：:KIF14 is a microtubule motor protein whose elevated expression is associated with poor-prognosis breast cancer. Here we demonstrate KIF14 accumulation in mitotic cells, where it associated with developing spindle poles and spindle microtubules. Cells at later stages of mitosis were characterized by the concentration o...

journal_title：Molecular and cellular biology

authors： Carleton M,Mao M,Biery M,Warrener P,Kim S,Buser C,Marshall CG,Fernandes C,Annis J,Linsley PS

更新日期：2006-05-01 00:00:00

abstract：:Eukaryotic genomes are divided into independent transcriptional domains by DNA elements known as insulators. The gypsy insulator, a 350-bp element isolated from the Drosophila gypsy retrovirus, contains twelve degenerate binding sites for the Suppressor of Hairy-wing [Su(Hw)] protein. Su(Hw) associates with over 500 n...

journal_title：Molecular and cellular biology

authors： Parnell TJ,Kuhn EJ,Gilmore BL,Helou C,Wold MS,Geyer PK

更新日期：2006-08-01 00:00:00

abstract：:After flagellar detachment in Chlamydomonas reinhardi, there is a rapid synthesis and accumulation of mRNAs for tubulin and other flagellar proteins. Maximum levels of these mRNAs (flagellar RNAs) are reached within 1 h after deflagellation, after which they are rapidly degraded to their predeflagellation levels. The ...

journal_title：Molecular and cellular biology

authors： Baker EJ,Keller LR,Schloss JA,Rosenbaum JL

更新日期：1986-01-01 00:00:00

abstract：:Wnt signaling is critical to many aspects of development, and aberrant activation of the Wnt signaling pathway can cause cancer. Dishevelled (Dvl) protein plays a central role in this pathway by transducing the signal from the Wnt receptor complex to the beta-catenin destruction complex. Dvl also plays a pivotal role ...

journal_title：Molecular and cellular biology

authors： Cong F,Schweizer L,Varmus H

更新日期：2004-03-01 00:00:00

abstract：:Precisely regulated expression of oncogenes and tumor suppressor genes is essential for normal development, and deregulated expression can lead to cancer. The human N-myc gene normally is expressed in only a subset of fetal epithelial tissues, and its expression is extinguished in all adult tissues except transiently ...

journal_title：Molecular and cellular biology

authors： Sivak LE,Pont-Kingdon G,Le K,Mayr G,Tai KF,Stevens BT,Carroll WL

更新日期：1999-01-01 00:00:00

abstract：:We have previously identified an 11-base DNA sequence, 5'-G-G-T-A-C-C-T-A-A-C-C-3' (simian virus 40 [SV40] map position 294 to 304), which is important in the control of SV40 late RNA expression in vitro and in vivo (Brady et al., Cell 31:625-633, 1982). We report here the identification of another domain of the SV40 ...

journal_title：Molecular and cellular biology

authors： Brady J,Radonovich M,Thoren M,Das G,Salzman NP

更新日期：1984-01-01 00:00:00

abstract：:We have examined the possible role of adenosine 3',5'-phosphate (cAMP) in functions associated with the plasma membranes of Saccharomyces cerevisiae. Purified membranes from this source contained an adenylate cyclase which was insensitive to activation by fluoride or guanine nucleotides, only weakly responsive to chan...

journal_title：Molecular and cellular biology

authors： Jaynes PK,McDonough JP,Mahler HR

更新日期：1982-12-01 00:00:00

abstract：:Expression of the Saccharomyces cerevisiae CYC1 gene produces mRNA with more than 20 different 5' ends. A derivative of the CYC1 gene (CYC1-157) was constructed with a deletion of a portion of the CYC1 5'-noncoding region, which includes the sites at which many of the CYC1 mRNAs 5' ends map. A 54-mer double-stranded o...

journal_title：Molecular and cellular biology

authors： McNeil JB,Smith M

更新日期：1985-12-01 00:00:00

abstract：:Plasmid DNA substrates were X-irradiated and injected into the nuclei of Xenopus laevis oocytes. After incubation for 20 h, DNA was recovered from the oocytes and analyzed simultaneously for repair and for intermolecular homologous recombination by electrophoresis and bacterial transformation. Oocyte-mediated repair o...

journal_title：Molecular and cellular biology

authors： Sweigert SE,Carroll D

更新日期：1990-11-01 00:00:00

abstract：:In order to create an extended map of chromatin features within a mammalian multigene locus, we have determined the extent of nuclease sensitivity and the pattern of histone modifications associated with the mouse beta-globin genes in adult erythroid tissue. We show that the nuclease-sensitive domain encompasses the b...

journal_title：Molecular and cellular biology

authors： Bulger M,Schübeler D,Bender MA,Hamilton J,Farrell CM,Hardison RC,Groudine M

更新日期：2003-08-01 00:00:00

abstract：:Methylation-controlled J protein (MCJ) is a newly identified member of the DnaJ family of cochaperones. Hypermethylation-mediated transcriptional silencing of the MCJ gene has been associated with increased chemotherapeutic resistance in ovarian cancer. However, the biology and function of MCJ remain unknown. Here we ...

journal_title：Molecular and cellular biology

authors： Hatle KM,Neveu W,Dienz O,Rymarchyk S,Barrantes R,Hale S,Farley N,Lounsbury KM,Bond JP,Taatjes D,Rincón M

更新日期：2007-04-01 00:00:00

abstract：:Intestinal gene regulation involves mechanisms that direct temporal expression along the vertical and horizontal axes of the alimentary tract. Sucrase-isomaltase (SI), the product of an enterocyte-specific gene, exhibits a complex pattern of expression. Generation of transgenic mice with a mutated SI transgene showed ...

journal_title：Molecular and cellular biology

authors： Boudreau F,Rings EH,Swain GP,Sinclair AM,Suh ER,Silberg DG,Scheuermann RH,Traber PG

更新日期：2002-08-01 00:00:00

abstract：:The c-Jun NH(2)-terminal kinase (JNK) group of mitogen-activated protein kinases (MAPKs) is activated in response to the treatment of cells with inflammatory cytokines and by exposure to environmental stress. JNK activation is mediated by a protein kinase cascade composed of a MAPK kinase and a MAPK kinase kinase. Her...

journal_title：Molecular and cellular biology

authors： Kelkar N,Gupta S,Dickens M,Davis RJ

更新日期：2000-02-01 00:00:00

abstract：:While wild-type p53 is normally a rapidly degraded protein, mutant forms of p53 are stabilized and accumulate to high levels in tumor cells. In this study, we show that mutant and wild-type p53 proteins are ubiquitinated and degraded through overlapping but distinct pathways. While Mdm2 can drive the degradation of bo...

journal_title：Molecular and cellular biology

authors： Lukashchuk N,Vousden KH

更新日期：2007-12-01 00:00:00

abstract：:mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 (YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap...

journal_title：Molecular and cellular biology

authors： Boeck R,Lapeyre B,Brown CE,Sachs AB

更新日期：1998-09-01 00:00:00

abstract：:The functional receptor for insect ecdysteroid hormones is a heterodimer consisting of two nuclear hormone receptors, ecdysteroid receptor (EcR) and the retinoid X receptor homologue Ultraspiracle (USP). Although ecdysone is commonly thought to be a hormone precursor and 20-hydroxyecdysone (20E), the physiologically a...

journal_title：Molecular and cellular biology

authors： Wang SF,Ayer S,Segraves WA,Williams DR,Raikhel AS

更新日期：2000-06-01 00:00:00

abstract：:A variant polyadenylation signal, which is conserved and employed by mammalian hepadnaviruses, has a sequence resembling that of the TATA box. We report here that this composite box manifests all the promoter characteristics. It binds effectively TATA-binding protein with TFIIB and TFIIA in a synergistic manner. This ...

journal_title：Molecular and cellular biology

authors： Paran N,Ori A,Haviv I,Shaul Y

更新日期：2000-02-01 00:00:00

abstract：:Much of the regulatory diversity in eukaryotic transcription is provided by coregulators, which are recruited by DNA-binding factors to propagate signaling to basal machinery or chromatin. p160 family members, including the glucocorticoid receptor (GR)-interacting protein 1 (GRIP1), function as coactivators for GR, a ...

journal_title：Molecular and cellular biology

authors： Dobrovolna J,Chinenov Y,Kennedy MA,Liu B,Rogatsky I

更新日期：2012-02-01 00:00:00

abstract：:COUP-TF, an orphan member of the nuclear receptor superfamily, has been proposed to play a key role in regulating organogenesis, neurogenesis, and cellular differentiation during embryonic development. Since heterodimierization is a common theme within the nuclear receptor superfamily and has been demonstrated to modu...

journal_title：Molecular and cellular biology

authors： Leng X,Cooney AJ,Tsai SY,Tsai MJ

更新日期：1996-05-01 00:00:00

abstract：:Cellular senescence is an irreversible growth arrest and is presumed to be a natural barrier to tumor development. Like telomere shortening, certain defects in chromosome integrity can trigger senescence; however, the roles of centromere proteins in regulating commitment to the senescent state remains to be establishe...

journal_title：Molecular and cellular biology

authors： Maehara K,Takahashi K,Saitoh S

更新日期：2010-05-01 00:00:00

abstract：:The carboxyl-terminal domain (CTD) of RNA polymerase II (Pol II) can be phosphorylated at serine 2 (Ser-2) and serine 5 (Ser-5) of the CTD heptad repeat YSPTSPS, and this phosphorylation is important in coupling transcription to RNA processing, including 5' capping, splicing, and polyadenylation. The mammalian endogen...

journal_title：Molecular and cellular biology

authors： Cheng C,Sharp PA

更新日期：2003-03-01 00:00:00

abstract：:The male-specific-lethal (MSL) proteins in Drosophila melanogaster serve to adjust gene expression levels in male flies containing a single X chromosome to equal those in females with a double dose of X-linked genes. Together with noncoding roX RNA, MSL proteins form the "dosage compensation complex" (DCC), which inte...

journal_title：Molecular and cellular biology

authors： Morales V,Regnard C,Izzo A,Vetter I,Becker PB

更新日期：2005-07-01 00:00:00

abstract：:Mitochondria are indispensable organelles implicated in multiple aspects of cellular processes, including tumorigenesis. Heat shock proteins play a critical regulatory role in accurately delivering the nucleus-encoded proteins through membrane-bound presequence translocase (Tim23 complex) machinery. Although altered e...

journal_title：Molecular and cellular biology

authors： Sinha D,Srivastava S,Krishna L,D'Silva P

更新日期：2014-05-01 00:00:00

abstract：:The mammalian double-stranded RNA-activated protein kinase PKR is a component of the cellular antiviral defense mechanism and phosphorylates Ser-51 on the alpha subunit of the translation factor eIF2 to inhibit protein synthesis. To identify the molecular determinants that specify substrate recognition by PKR, we perf...

journal_title：Molecular and cellular biology

authors： Kawagishi-Kobayashi M,Silverman JB,Ung TL,Dever TE

更新日期：1997-07-01 00:00:00

abstract：:The niaD and niiA genes of Aspergillus nidulans, which code, respectively, for nitrate and nitrite reductases, are divergently transcribed, and their ATGs are separated by 1,200 bp. The genes are under the control of the positively acting NirA transcription factor, which mediates nitrate induction. The DNA binding dom...

journal_title：Molecular and cellular biology

authors： Punt PJ,Strauss J,Smit R,Kinghorn JR,van den Hondel CA,Scazzocchio C

更新日期：1995-10-01 00:00:00

abstract：:RSC is an essential chromatin remodeling complex in Saccharomyces cerevisiae that performs central roles in transcriptional regulation and cell cycle progression. Here we identify Htl1 as a novel factor that associates with the RSC complex both physically and functionally. We isolated HTL1 through a genetic screen for...

journal_title：Molecular and cellular biology

authors： Romeo MJ,Angus-Hill ML,Sobering AK,Kamada Y,Cairns BR,Levin DE

更新日期：2002-12-01 00:00:00