Saccharomyces cerevisiae CYC1 mRNA 5'-end positioning: analysis by in vitro mutagenesis, using synthetic duplexes with random mismatch base pairs.

abstract：:Mouse erythroleukemia cells contain several abundant mRNA species that occur to a considerable extent as untranslated molecules. For two of these species, which code for polypeptides P40 and P21, the proportion of molecules engaged in translation decreases rapidly after exposure of the cells to dimethyl sulfoxide. The...

journal_title：Molecular and cellular biology

authors： Yenofsky R,Cereghini S,Krowczynska A,Brawerman G

更新日期：1983-07-01 00:00:00

abstract：:To examine the functional role of the interaction between Munc18c and syntaxin 4 in the regulation of GLUT4 translocation in 3T3L1 adipocytes, we assessed the effects of introducing three different peptide fragments (20 to 24 amino acids) of Munc18c from evolutionarily conserved regions of the Sec1 protein family pred...

journal_title：Molecular and cellular biology

authors： Thurmond DC,Kanzaki M,Khan AH,Pessin JE

更新日期：2000-01-01 00:00:00

abstract：:We have previously described an isolated kinetoplast system from Crithidia fasciculata capable of ATP-dependent replication of kinetoplast DNA minicircles (L. Birkenmeyer and D.S. Ray, J. Biol. Chem. 261: 2362-2368, 1986). We present here the identification of two new minicircle species observed in short pulse-labelin...

journal_title：Molecular and cellular biology

authors： Sheline C,Melendy T,Ray DS

更新日期：1989-01-01 00:00:00

abstract：:CD133, a widely known marker of cancer stem cells, was recently found in extracellular vesicles. However, the mechanisms underlying CD133 translocation to the extracellular space remain largely unknown. Here we report that CD133 is monoubiquitinated. Ubiquitination occurs primarily on complex glycosylated CD133. The l...

journal_title：Molecular and cellular biology

authors： Yang F,Xing Y,Li Y,Chen X,Jiang J,Ai Z,Wei Y

更新日期：2018-07-16 00:00:00

abstract：:Fragments of African green monkey (Cercopithecus aethiops) DNA (3.5 to 18.0 kilobases) were inserted downstream from the thymidine kinase (TK, tk) coding region in pTK206/SV010, a gene construct which lacks both copies of the hexanucleotide 5'-AATAAA-3' and contains a simian virus 40 origin of replication, allowing it...

journal_title：Molecular and cellular biology

authors： Santangelo GM,Cole CN

更新日期：1983-04-01 00:00:00

abstract：:Xrn1p of Saccharomyces cerevisiae is a major cytoplasmic RNA turnover exonuclease which is evolutionarily conserved from yeasts to mammals. Deletion of the XRN1 gene causes pleiotropic phenotypes, which have been interpreted as indirect consequences of the RNA turnover defect. By sequence comparisons, we have identifi...

journal_title：Molecular and cellular biology

authors： Solinger JA,Pascolini D,Heyer WD

更新日期：1999-09-01 00:00:00

abstract：:Liu and Mertz (Genes Dev. 9:1766-1780, 1995) previously identified a 119-nt pre-mRNA processing enhancer (PPE) element within the herpes simplex virus type 1 thymidine kinase gene that enables intron-independent gene expression in higher eukaryotes by binding heterogeneous nuclear ribonucleoprotein L (hnRNP L). Here, ...

journal_title：Molecular and cellular biology

authors： Guang S,Felthauser AM,Mertz JE

更新日期：2005-08-01 00:00:00

abstract：:Several factors that mediate activation by nuclear receptors also modify the chemical and structural composition of chromatin. Prominent in this diverse group is the steroid receptor coactivator 1 (SRC-1) family, which interact with agonist-bound nuclear receptors, thereby coupling them to multifunctional transcriptio...

journal_title：Molecular and cellular biology

authors： DiRenzo J,Shang Y,Phelan M,Sif S,Myers M,Kingston R,Brown M

更新日期：2000-10-01 00:00:00

abstract：:Recent studies showed that cyclophilin A (CypA) promotes NF-κB/p65 nuclear translocation, resulting in enhanced NF-κB activity and altered expression of its target genes, such as the Sox9 transcriptional factor, which plays a critical role in chondrogenic differentiation and endochondral ossification. In this report, ...

journal_title：Molecular and cellular biology

authors： Guo M,Shen J,Kwak JH,Choi B,Lee M,Hu S,Zhang X,Ting K,Soo CB,Chiu RH

更新日期：2015-06-01 00:00:00

abstract：:Ribosome biogenesis is a highly regulated process ensuring that cell growth (increase in biomass) is coordinated with cell proliferation. The formation of eukaryotic ribosomes is a multistep process initiated by the transcription and processing of rRNA in the nucleolus. Concomitant with this, several preribosomal part...

journal_title：Molecular and cellular biology

authors： Castle CD,Cassimere EK,Lee J,Denicourt C

更新日期：2010-09-01 00:00:00

abstract：:Costimulation of G protein-coupled receptors (GPCRs) may result in cross talk interactions between their downstream signaling pathways. Stimulation of GPCRs may also lead to cross talk regulation of receptor tyrosine kinase signaling and thereby to activation of mitogen-activated protein kinase (MAPK). In COS-7 cells,...

journal_title：Molecular and cellular biology

authors： Hanke S,Nürnberg B,Groll DH,Liebmann C

更新日期：2001-12-01 00:00:00

abstract：:The active 17S U2 small nuclear ribonucleoprotein particle (snRNP), which binds to the intron branch site during the formation of the prespliceosome, is assembled in vitro by sequential interactions of the essential splicing factors SF3b and SF3a with the 12S U2 snRNP. We have analyzed the function of individual subun...

journal_title：Molecular and cellular biology

authors： Nesic D,Krämer A

更新日期：2001-10-01 00:00:00

abstract：:KIF14 is a microtubule motor protein whose elevated expression is associated with poor-prognosis breast cancer. Here we demonstrate KIF14 accumulation in mitotic cells, where it associated with developing spindle poles and spindle microtubules. Cells at later stages of mitosis were characterized by the concentration o...

journal_title：Molecular and cellular biology

authors： Carleton M,Mao M,Biery M,Warrener P,Kim S,Buser C,Marshall CG,Fernandes C,Annis J,Linsley PS

更新日期：2006-05-01 00:00:00

abstract：:The fgr proto-oncogene encodes a nonreceptor protein-tyrosine kinase, designated p55c-fgr. In this study, we have isolated human fgr cDNA molecules from normal monocyte mRNA templates. Nucleotide sequence analysis of the longest fgr cDNA revealed a 5' untranslated region of 927 bp which included two Alu-like repeats a...

journal_title：Molecular and cellular biology

authors： Gutkind JS,Link DC,Katamine S,Lacal P,Miki T,Ley TJ,Robbins KC

更新日期：1991-03-01 00:00:00

abstract：:For proper male sexual differentiation, anti-Müllerian hormone (AMH) must be tightly regulated during embryonic development to promote regression of the Müllerian duct. However, the molecular mechanisms specifying the onset of AMH in male mammals are not yet clearly defined. A DNA-binding element for the steroidogenic...

journal_title：Molecular and cellular biology

authors： De Santa Barbara P,Bonneaud N,Boizet B,Desclozeaux M,Moniot B,Sudbeck P,Scherer G,Poulat F,Berta P

更新日期：1998-11-01 00:00:00

abstract：:Human La protein has been shown to serve as a transcription factor for RNA polymerase III (pol III) by facilitating transcription termination and recycling of transcription complexes. In addition, La binds to the 3' oligo(U) ends common to all nascent pol III transcripts, and in the case of B1-Alu RNA, protects it fro...

journal_title：Molecular and cellular biology

authors： Goodier JL,Fan H,Maraia RJ

更新日期：1997-10-01 00:00:00

abstract：:In addition to the highly conserved AATAAA sequence, there is a requirement for specific sequences downstream of polyadenylic acid [poly(A)] cleavage sites to generate correct mRNA 3' termini. Previous experiments demonstrated that 35 nucleotides downstream of the E2A poly(A) site were sufficient but 20 nucleotides we...

journal_title：Molecular and cellular biology

authors： Hart RP,McDevitt MA,Ali H,Nevins JR

更新日期：1985-11-01 00:00:00

abstract：:YY1 is a multifunctional transcription factor that acts as an activator or repressor in different contexts. YY1 binds to multiple sites in the mouse c-fos promoter, inducing at each site a sharp DNA bend. Binding of YY1 to a site situated between the cyclic AMP response element (CRE) and the TATA box bends the DNA in ...

journal_title：Molecular and cellular biology

authors： Natesan S,Gilman M

更新日期：1995-11-01 00:00:00

abstract：:Ras proteins can activate at least three classes of downstream target proteins: Raf kinases, phosphatidylinositol-3 phosphate (PI3) kinase, and Ral-specific guanine nucleotide exchange factors (Ral-GEFs). In NIH 3T3 cells, activated Ral-GEFs contribute to Ras-induced cell proliferation and oncogenic transformation by ...

journal_title：Molecular and cellular biology

authors： Goi T,Rusanescu G,Urano T,Feig LA

更新日期：1999-03-01 00:00:00

abstract：:Mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) has been shown to play a critical role in mediating the feedback control of MAP kinase cascades in a variety of cellular processes, including proliferation and stress responsiveness. Although MKP-1 expression is induced by a broad array of extracellular stim...

journal_title：Molecular and cellular biology

authors： Li J,Gorospe M,Hutter D,Barnes J,Keyse SM,Liu Y

更新日期：2001-12-01 00:00:00

abstract：:The ARK (AXL, UFO) receptor is a member of a new family of receptor tyrosine kinases whose extracellular domain contains a combination of fibronectin type III and immunoglobulin motifs similar to those found in many cell adhesion molecules. ARK mRNA is expressed at high levels in the mouse brain, prevalently in the hi...

journal_title：Molecular and cellular biology

authors： Bellosta P,Costa M,Lin DA,Basilico C

更新日期：1995-02-01 00:00:00

abstract：:Murine embryonic stem (ES) cells are defined by continuous self-renewal and pluripotency. A diverse repertoire of protein isoforms arising from alternative splicing is expressed in ES cells without defined biological roles. Sall4, a transcription factor essential for pluripotency, exists as two isoforms (Sall4a and Sa...

journal_title：Molecular and cellular biology

authors： Rao S,Zhen S,Roumiantsev S,McDonald LT,Yuan GC,Orkin SH

更新日期：2010-11-01 00:00:00

abstract：:Maintaining optimal bone integrity, mass, and strength throughout adult life requires ongoing bone remodeling, which involves coordinated activity between actions of bone-resorbing osteoclasts and bone forming-osteoblasts. Osteoporosis is a disorder of remodeling in which bone resorption outstrips deposition, leading ...

journal_title：Molecular and cellular biology

authors： Mukherjee A,Rotwein P

更新日期：2012-01-01 00:00:00

abstract：:Illegitimate recombination events in mammalian cells often contain extraneous nucleotides or filler DNA at the recombinant joins. The polyomavirus-transformed cell line 7axB has previously been found to contain 37 base pairs (bp) of filler DNA at one virus-host join of the single insert of integrated viral DNA (A. Hay...

journal_title：Molecular and cellular biology

authors： Williams TJ,Fried M

更新日期：1986-06-01 00:00:00

abstract：:The LAR family protein tyrosine phosphatases (PTPs), including LAR, PTP delta, and PTP sigma, are transmembrane proteins composed of a cell adhesion molecule-like ectodomain and two cytoplasmic catalytic domains: active D1 and inactive D2. We performed a yeast two-hybrid screen with the first catalytic domain of PTP s...

journal_title：Molecular and cellular biology

authors： Wallace MJ,Fladd C,Batt J,Rotin D

更新日期：1998-05-01 00:00:00

abstract：:RNA polymerase II transcribes the mRNA-encoding genes and the majority of the small nuclear RNA (snRNA) genes. The formation of a minimal functional transcription initiation complex on a TATA-box-containing mRNA promoter has been well characterized and involves the ordered assembly of a number of general transcription...

journal_title：Molecular and cellular biology

authors： Kuhlman TC,Cho H,Reinberg D,Hernandez N

更新日期：1999-03-01 00:00:00

abstract：:Transfection of a hamster cell mutant with human DNA corrected both the low-density lipoprotein receptor-deficient phenotype and the multiple glycosylation defects of the cells. Independently transfected colonies contained a small set of common human DNA fragments. These fragments may correspond to the human analog of...

journal_title：Molecular and cellular biology

authors： Kingsley DM,Sege RD,Kozarsky KF,Krieger M

更新日期：1986-07-01 00:00:00

abstract：:Neuronal differentiation is tightly regulated by a variety of factors. In a search for neuron-specific genes, we identified a highly conserved novel zinc finger protein, ZNF536. We observed that ZNF536 is most abundant in the brain and, in particular, is expressed in the developing central nervous system and dorsal ro...

journal_title：Molecular and cellular biology

authors： Qin Z,Ren F,Xu X,Ren Y,Li H,Wang Y,Zhai Y,Chang Z

更新日期：2009-07-01 00:00:00

abstract：:Tristetraprolin (TTP) acts by binding to AU-rich elements in certain mRNAs, such as tumor necrosis factor (TNF) mRNA, and increasing their decay rates. TTP knockout mice exhibit a profound inflammatory syndrome that is largely due to increased TNF levels. Although TTP's effects on gene expression have been well studie...

journal_title：Molecular and cellular biology

authors： Patial S,Stumpo DJ,Young WS 3rd,Ward JM,Flake GP,Blackshear PJ

更新日期：2016-04-15 00:00:00

abstract：:DNA elements with the CC(A/T)6GG, or CArG, motif occur in promoters that are under different regulatory controls. CArG elements from the skeletal actin, c-fos, and myogenin genes were tested for their abilities to confer tissue-specific expression on reporter genes when the individual elements were situated immediatel...

journal_title：Molecular and cellular biology

authors： Santoro IM,Walsh K

更新日期：1991-12-01 00:00:00