Abstract:
:Neuronal differentiation is tightly regulated by a variety of factors. In a search for neuron-specific genes, we identified a highly conserved novel zinc finger protein, ZNF536. We observed that ZNF536 is most abundant in the brain and, in particular, is expressed in the developing central nervous system and dorsal root ganglia and localized in the cerebral cortex, hippocampus, and hypothalamic area. During neuronal differentiation of P19 cells induced by retinoic acid (RA), ZNF536 expression is increased at an early stage, and it is maintained at a constant level in later stages. Overexpression of ZNF536 results in an inhibition of RA-induced neuronal differentiation, while depletion or mutation of the ZNF536 gene results in an enhancement of differentiation. We further demonstrated that ZNF536 inhibits expression of neuron-specific marker genes, possibly through the inhibition of RA response element-mediated transcriptional activity, as overexpression of RA receptor alpha can rescue the inhibitory role of ZNF536 in neuronal differentiation and neuron-specific gene expression. Our studies have identified a novel zinc finger protein that negatively regulates neuron differentiation.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Qin Z,Ren F,Xu X,Ren Y,Li H,Wang Y,Zhai Y,Chang Zdoi
10.1128/MCB.00362-09subject
Has Abstractpub_date
2009-07-01 00:00:00pages
3633-43issue
13eissn
0270-7306issn
1098-5549pii
MCB.00362-09journal_volume
29pub_type
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