Abstract:
:Interleukin-6 (IL-6) and leukemia inhibitory factor (LIF), two multifunctional cytokines, recently have been identified as physiological inducers of hematopoietic cell differentiation which also induce terminal differentiation and growth arrest of the myeloblastic leukemic M1 cell line. In this work, it is shown that c-myc exhibited a unique pattern of expression upon induction of M1 terminal differentiation by LIF or IL-6, with an early transient increase followed by a decrease to control levels by 12 h and no detectable c-myc mRNA by 1 day; in contrast, c-myb expression was rapidly suppressed, with no detectable c-myb mRNA by 12 h. Vectors containing the c-myc gene under control of the beta-actin gene promoter were transfected into M1 cells to obtain M1myc cell lines which constitutively synthesized c-myc. Deregulated and continued expression of c-myc blocked terminal differentiation induced by IL-6 or LIF at an intermediate stage in the progression from immature blasts to mature macrophages, precisely at the point in time when c-myc is normally suppressed, leading to intermediate-stage myeloid cells which continued to proliferate in the absence of c-myb expression.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Hoffman-Liebermann B,Liebermann DAdoi
10.1128/mcb.11.5.2375subject
Has Abstractpub_date
1991-05-01 00:00:00pages
2375-81issue
5eissn
0270-7306issn
1098-5549journal_volume
11pub_type
杂志文章abstract::The human tumor necrosis factor alpha (TNF-alpha) gene is rapidly activated in response to multiple signals of stress and inflammation. We have identified transcription factors present in the TNF-alpha enhancer complex in vivo following ionophore stimulation (ATF-2/Jun and NFAT) and virus infection (ATF-2/Jun, NFAT, a...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/mcb.7.3.1101
更新日期:1987-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.15.4.1870
更新日期:1995-04-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00569-16
更新日期:2017-08-11 00:00:00
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pub_type: 杂志文章
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更新日期:1986-04-01 00:00:00
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journal_title:Molecular and cellular biology
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更新日期:1988-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-07-01 00:00:00