axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase.


:Using a sensitive transfection-tumorigenicity assay, we have isolated a novel transforming gene from the DNA of two patients with chronic myelogenous leukemia. Sequence analysis indicates that the product of this gene, axl, is a receptor tyrosine kinase. Overexpression of axl cDNA in NIH 3T3 cells induces neoplastic transformation with the concomitant appearance of a 140-kDa axl tyrosine-phosphorylated protein. Expression of axl cDNA in the baculovirus system results in the expression of the appropriate recombinant protein that is recognized by antiphosphotyrosine antibodies, confirming that the axl protein is a tyrosine kinase. The juxtaposition of fibronectin type III and immunoglobulinlike repeats in the extracellular domain, as well as distinct amino acid sequences in the kinase domain, indicate that the axl protein represents a novel subclass of receptor tyrosine kinases.


Mol Cell Biol


O'Bryan JP,Frye RA,Cogswell PC,Neubauer A,Kitch B,Prokop C,Espinosa R 3rd,Le Beau MM,Earp HS,Liu ET




Has Abstract


1991-10-01 00:00:00












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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Myers PL,Skvirsky RC,Greenberg ML,Greer H

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    pub_type: 杂志文章


    authors: Majumder P,Boss JM

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    pub_type: 杂志文章


    authors: Selvakumaran M,Lin HK,Sjin RT,Reed JC,Liebermann DA,Hoffman B

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


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    更新日期:1991-09-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Swarbrick A,Lee CS,Sutherland RL,Musgrove EA

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bouvet P,Paris J,Phillippe M,Osborne HB

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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