axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase.

Abstract:

:Using a sensitive transfection-tumorigenicity assay, we have isolated a novel transforming gene from the DNA of two patients with chronic myelogenous leukemia. Sequence analysis indicates that the product of this gene, axl, is a receptor tyrosine kinase. Overexpression of axl cDNA in NIH 3T3 cells induces neoplastic transformation with the concomitant appearance of a 140-kDa axl tyrosine-phosphorylated protein. Expression of axl cDNA in the baculovirus system results in the expression of the appropriate recombinant protein that is recognized by antiphosphotyrosine antibodies, confirming that the axl protein is a tyrosine kinase. The juxtaposition of fibronectin type III and immunoglobulinlike repeats in the extracellular domain, as well as distinct amino acid sequences in the kinase domain, indicate that the axl protein represents a novel subclass of receptor tyrosine kinases.

journal_name

Mol Cell Biol

authors

O'Bryan JP,Frye RA,Cogswell PC,Neubauer A,Kitch B,Prokop C,Espinosa R 3rd,Le Beau MM,Earp HS,Liu ET

doi

10.1128/mcb.11.10.5016

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

5016-31

issue

10

eissn

0270-7306

issn

1098-5549

journal_volume

11

pub_type

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