Abstract:
:Hybridization to synthetic oligonucleotides representing conserved regions in the promoter and first intron of several vertebrate beta-actin genes was used to discriminate between what appears to be a single functional beta-actin gene and numerous pseudogenes in the mouse genome. Sequences derived from the 5' end of this gene were shown to confer serum-inducible expression upon a heterologous reporter gene when transfected into mouse fibroblasts. Moreover, these sequences rendered reporter gene expression superinducible by a combination of serum and cycloheximide. These experiments indicate that the 5' end of the mouse beta-actin gene contains sequence elements which mediate the stimulatory effects of serum growth factors and which are responsive to both positive and negative regulators of gene expression.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Elder PK,French CL,Subramaniam M,Schmidt LJ,Getz MJdoi
10.1128/mcb.8.1.480subject
Has Abstractpub_date
1988-01-01 00:00:00pages
480-5issue
1eissn
0270-7306issn
1098-5549journal_volume
8pub_type
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