Microcell-mediated cotransfer of genes specifying methotrexate resistance, emetine sensitivity, and chromate sensitivity with Chinese hamster chromosome 2.


:Many selectable mutants of somatic Chinese hamster cells have been described, but very few of the mutations have been mapped to specific chromosomes. We have utilized the microcell-mediated gene transfer technique to establish the location of three selectable genetic markers on chromosome 2 of Chinese hamster. Microcells were prepared from the methotrexate-resistant MtxRIII line of Flintoff et al. (Somatic Cell Genet. 2:245-261, 1976) and fused to wild-type CHO cells, and microcell hybrids (transferants) were selected in medium containing methotrexate. All transferants were karyotyped and found to contain a marker chromosome from the donor MtxRIII line. This marker chromosome, called 2p-, consisted of a chromosome 2 with a reduced short arm resulting from a reciprocal translocation between 2p and 5q. In experiments utilizing emetine-resistant (Emtr) or chromate-resistant (Chrr) recipient cells it was found that the emt+ and chr+ wild-type genes were cotransferred with the 2p- chromosomes. Karyotype analysis of several transferants with rearranged or broken 2p- markers allowed regional localization of the emt and chr loci to the proximal third of the long arm and localization of the gene or genes conferring methotrexate resistance to the short arm. These results confirm our earlier assignment of the emt and chr loci to chromosome 2 in Chinese hamster.


Mol Cell Biol


Worton R,Duff C,Flintoff W




Has Abstract


1981-04-01 00:00:00












  • Alkylpurine-DNA-N-glycosylase knockout mice show increased susceptibility to induction of mutations by methyl methanesulfonate.

    abstract::Alkylpurine-DNA-N-glycosylase (APNG) null mice have been generated by homologous recombination in embryonic stem cells. The null status of the animals was confirmed at the mRNA level by reverse transcription-PCR and by the inability of cell extracts of tissues from the knockout (ko) animals to release 3-methyladenine ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Elder RH,Jansen JG,Weeks RJ,Willington MA,Deans B,Watson AJ,Mynett KJ,Bailey JA,Cooper DP,Rafferty JA,Heeran MC,Wijnhoven SW,van Zeeland AA,Margison GP

    更新日期:1998-10-01 00:00:00

  • Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin.

    abstract::Wnt signaling is thought to be mediated via interactions between beta-catenin and members of the LEF-1/TCF family of transcription factors. Here we study the mechanism of transcriptional regulation by LEF-1 in response to a Wnt-1 signal under conditions of endogenous beta-catenin in NIH 3T3 cells, and we examine wheth...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hsu SC,Galceran J,Grosschedl R

    更新日期:1998-08-01 00:00:00

  • Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways.

    abstract::TRAIL is an endogenous death receptor ligand also used therapeutically because of its selective proapoptotic activity in cancer cells. In the present study, we examined chromatin alterations induced by TRAIL and show that TRAIL induces a rapid activation of DNA damage response (DDR) pathways with histone H2AX, Chk2, A...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Solier S,Sordet O,Kohn KW,Pommier Y

    更新日期:2009-01-01 00:00:00

  • Alteration of NCoR corepressor splicing in mice causes increased body weight and hepatosteatosis without glucose intolerance.

    abstract::Alternative mRNA splicing is an important means of diversifying function in higher eukaryotes. Notably, both NCoR and SMRT corepressors are subject to alternative mRNA splicing, yielding a series of distinct corepressor variants with highly divergent functions. Normal adipogenesis is associated with a switch in corepr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Goodson ML,Young BM,Snyder CA,Schroeder AC,Privalsky ML

    更新日期:2014-11-15 00:00:00

  • A kinase-negative mutant of S49 mouse lymphoma cells is defective in posttranslational maturation of catalytic subunit of cyclic AMP-dependent protein kinase.

    abstract::Kinase-negative mutants of S49 mouse lymphoma cells, which lack detectable catalytic (C) subunit of cyclic AMP-dependent protein kinase, nevertheless contain cytoplasmic mRNAs for the two major forms of C subunit, C alpha and C beta. Investigation of the metabolism of C subunits in wild-type and mutant cells was under...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Steinberg RA

    更新日期:1991-02-01 00:00:00

  • ARTD1 Suppresses Interleukin 6 Expression by Repressing MLL1-Dependent Histone H3 Trimethylation.

    abstract::ADP-ribosyltransferase diphtheria-toxin like 1/poly(ADP-ribose) polymerase 1 (ARTD1/PARP1) is a chromatin-associated protein in the nucleus and plays an important role in different cellular processes such as regulation of gene transcription. ARTD1 has been shown to coregulate the inflammatory response by modulating th...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Minotti R,Andersson A,Hottiger MO

    更新日期:2015-09-01 00:00:00

  • Regulation of histone mRNA production and stability in serum-stimulated mouse 3T6 fibroblasts.

    abstract::We measured the content and metabolism of histone mRNA in mouse 3T6 fibroblasts during a serum-induced transition from the resting to growing state. The content of several histone H3 and H2b mRNAs was measured by an S1 nuclease procedure. All of these increase in parallel by a factor of about 50 during S phase. Howeve...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: DeLisle AJ,Graves RA,Marzluff WF,Johnson LF

    更新日期:1983-11-01 00:00:00

  • Vanin-1-/- mice exhibit a glutathione-mediated tissue resistance to oxidative stress.

    abstract::Vanin-1 is an epithelial ectoenzyme with pantetheinase activity and generating the amino-thiol cysteamine through the metabolism of pantothenic acid (vitamin B(5)). Here we show that Vanin-1(-/-) mice, which lack cysteamine in tissues, exhibit resistance to oxidative injury induced by whole-body gamma-irradiation or p...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Berruyer C,Martin FM,Castellano R,Macone A,Malergue F,Garrido-Urbani S,Millet V,Imbert J,Duprè S,Pitari G,Naquet P,Galland F

    更新日期:2004-08-01 00:00:00

  • The nuclear form of phospholipid hydroperoxide glutathione peroxidase is a protein thiol peroxidase contributing to sperm chromatin stability.

    abstract::The selenoenzyme phospholipid hydroperoxide glutathione peroxidase (PHGPx) is regarded as the major molecular target of selenodeficiency in rodents, accounting for most of the histopathological and structural abnormalities of testicular tissue and male germ cells. PHGPx exists as a cytosolic form, mitochondrial form, ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Conrad M,Moreno SG,Sinowatz F,Ursini F,Kölle S,Roveri A,Brielmeier M,Wurst W,Maiorino M,Bornkamm GW

    更新日期:2005-09-01 00:00:00

  • IkappaB kinase beta phosphorylates the K63 deubiquitinase A20 to cause feedback inhibition of the NF-kappaB pathway.

    abstract::Misregulation of NF-kappaB signaling leads to infectious, inflammatory, or autoimmune disorders. IkappaB kinase beta (IKKbeta) is an essential activator of NF-kappaB and is known to phosphorylate the NF-kappaB inhibitor, IkappaBalpha, allowing it to undergo ubiquitin-mediated proteasomal degradation. However, beyond I...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hutti JE,Turk BE,Asara JM,Ma A,Cantley LC,Abbott DW

    更新日期:2007-11-01 00:00:00

  • Residual nitrobenzylthioinosine-resistant nucleoside transport in a transport mutant (AE1) of S49 murine T-lymphoma cells.

    abstract::The uptake of various nucleosides by S49 mouse T-lymphoma cells and that by a single-step nucleoside transport-defective mutant thereof (AE1) were compared. Residual nucleoside entry into AE1 cells occurred via two routes, nonmediated permeation and saturable, non-concentrative transport with broad substrate specifici...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Plagemann PG,Woffendin C

    更新日期:1987-01-01 00:00:00

  • The anchor site of telomerase from Euplotes aediculatus revealed by photo-cross-linking to single- and double-stranded DNA primers.

    abstract::Telomerase is a ribonucleoprotein enzyme that adds telomeric sequence repeats to the ends of linear chromosomes. In vitro, telomerase has been observed to add repeats to a DNA oligonucleotide primer in a processive manner, leading to the postulation of a DNA anchor site separate from the catalytic site of the enzyme. ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hammond PW,Lively TN,Cech TR

    更新日期:1997-01-01 00:00:00

  • Ligand-independent phosphorylation of the glucocorticoid receptor integrates cellular stress pathways with nuclear receptor signaling.

    abstract::Glucocorticoids are stress hormones that maintain homeostasis through gene regulation mediated by nuclear receptors. We have discovered that other cellular stressors are integrated with glucocorticoid signaling through a new hormone-independent phosphorylation site, Ser134, on the human glucocorticoid receptor (GR). S...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Galliher-Beckley AJ,Williams JG,Cidlowski JA

    更新日期:2011-12-01 00:00:00

  • Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1.

    abstract::Synonymous single nucleotide polymorphisms (SNPs) within a transcript's coding region produce no change in the amino acid sequence of the protein product and are therefore intuitively assumed to have a neutral effect on protein function. We report that two common variants of high-temperature requirement A1 (HTRA1) tha...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Jacobo SM,Deangelis MM,Kim IK,Kazlauskas A

    更新日期:2013-05-01 00:00:00

  • A human globin enhancer causes both discrete and widespread alterations in chromatin structure.

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kim A,Dean A

    更新日期:2003-11-01 00:00:00

  • DNA damage induction of ribonucleotide reductase.

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Elledge SJ,Davis RW

    更新日期:1989-11-01 00:00:00

  • Altered RNA editing in mice lacking ADAR2 autoregulation.

    abstract::ADAR2 is a double-stranded-RNA-specific adenosine deaminase involved in the editing of mammalian RNAs by the site-selective conversion of adenosine to inosine. Previous studies from our laboratory have demonstrated that ADAR2 can modify its own pre-mRNA to create a proximal 3' splice site containing a noncanonical ade...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Feng Y,Sansam CL,Singh M,Emeson RB

    更新日期:2006-01-01 00:00:00

  • The 5' guanosine tracts of human telomerase RNA are recognized by the G-quadruplex binding domain of the RNA helicase DHX36 and function to increase RNA accumulation.

    abstract::Telomerase promotes telomere maintenance by copying a template within its integral RNA subunit to elongate chromosome ends with new telomeric repeats. Motifs have been defined within the telomerase RNA that contribute to mature RNA accumulation, holoenzyme catalytic activity, or enzyme recruitment to telomeres. Here, ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sexton AN,Collins K

    更新日期:2011-02-01 00:00:00

  • Phosphorylation of mammalian eukaryotic translation initiation factor 6 and its Saccharomyces cerevisiae homologue Tif6p: evidence that phosphorylation of Tif6p regulates its nucleocytoplasmic distribution and is required for yeast cell growth.

    abstract::The synthesis of 60S ribosomal subunits in Saccharomyces cerevisiae requires Tif6p, the yeast homologue of mammalian eukaryotic translation initiation factor 6 (eIF6). In the present work, we have isolated a protein kinase from rabbit reticulocyte lysates on the basis of its ability to phosphorylate recombinant human ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Basu U,Si K,Deng H,Maitra U

    更新日期:2003-09-01 00:00:00

  • Characterization of NF-kappa B/I kappa B proteins in zebra fish and their involvement in notochord development.

    abstract::Although largely involved in innate and adaptive immunity, NF-kappa B plays an important role in vertebrate development. In chicks, the inactivation of the NF-kappa B pathway induces functional alterations of the apical ectodermal ridge, which mediates limb outgrowth. In mice, the complete absence of NF-kappa B activi...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Correa RG,Tergaonkar V,Ng JK,Dubova I,Izpisua-Belmonte JC,Verma IM

    更新日期:2004-06-01 00:00:00

  • Regulation of c-myc mRNA decay by translational pausing in a coding region instability determinant.

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Lemm I,Ross J

    更新日期:2002-06-01 00:00:00

  • Immune functions in mice lacking Clnk, an SLP-76-related adaptor expressed in a subset of immune cells.

    abstract::The SLP-76 family of immune cell-specific adaptors is composed of three distinct members named SLP-76, Blnk, and Clnk. They have been implicated in the signaling pathways coupled to immunoreceptors such as the antigen receptors and Fc receptors. Previous studies using gene-targeted mice and deficient cell lines showed...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Utting O,Sedgmen BJ,Watts TH,Shi X,Rottapel R,Iulianella A,Lohnes D,Veillette A

    更新日期:2004-07-01 00:00:00

  • An electrophoretic karyotype of Neurospora crassa.

    abstract::A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated s...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Orbach MJ,Vollrath D,Davis RW,Yanofsky C

    更新日期:1988-04-01 00:00:00

  • The LIM protein AJUBA recruits protein arginine methyltransferase 5 to mediate SNAIL-dependent transcriptional repression.

    abstract::The SNAIL transcription factor contains C-terminal tandem zinc finger motifs and an N-terminal SNAG repression domain. The members of the SNAIL family have recently emerged as major contributors to the processes of development and metastasis via the regulation of epithelial-mesenchymal transition events during embryon...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hou Z,Peng H,Ayyanathan K,Yan KP,Langer EM,Longmore GD,Rauscher FJ 3rd

    更新日期:2008-05-01 00:00:00

  • Positive and negative regulatory elements mediating transcription from the Drosophila melanogaster actin 5C distal promoter.

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Chung YT,Keller EB

    更新日期:1990-12-01 00:00:00

  • The oncoprotein kinase chaperone CDC37 functions as an oncogene in mice and collaborates with both c-myc and cyclin D1 in transformation of multiple tissues.

    abstract::CDC37 encodes a 50-kDa protein that targets intrinsically unstable oncoprotein kinases including Cdk4, Raf-1, and v-src to the molecular chaperone Hsp90, an interaction that is thought to be important for the establishment of signaling pathways. CDC37 is required for proliferation in budding yeast and is coexpressed w...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Stepanova L,Finegold M,DeMayo F,Schmidt EV,Harper JW

    更新日期:2000-06-01 00:00:00

  • Characterization of a family of related cellular transcription factors which can modulate human immunodeficiency virus type 1 transcription in vitro.

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Yoon JB,Li G,Roeder RG

    更新日期:1994-03-01 00:00:00

  • Selective cooperation between fatty acid binding proteins and peroxisome proliferator-activated receptors in regulating transcription.

    abstract::Lipophilic compounds such as retinoic acid and long-chain fatty acids regulate gene transcription by activating nuclear receptors such as retinoic acid receptors (RARs) and peroxisome proliferator-activated receptors (PPARs). These compounds also bind in cells to members of the family of intracellular lipid binding pr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Tan NS,Shaw NS,Vinckenbosch N,Liu P,Yasmin R,Desvergne B,Wahli W,Noy N

    更新日期:2002-07-01 00:00:00

  • Cooperation between the INO80 complex and histone chaperones determines adaptation of stress gene transcription in the yeast Saccharomyces cerevisiae.

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Klopf E,Paskova L,Solé C,Mas G,Petryshyn A,Posas F,Wintersberger U,Ammerer G,Schüller C

    更新日期:2009-09-01 00:00:00

  • Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast.

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Schmelzle T,Helliwell SB,Hall MN

    更新日期:2002-03-01 00:00:00