Meiotic induction by Xenopus cyclin B is accelerated by coexpression with mosXe.

Abstract:

:We have investigated the relationship between Xenopus laevis c-mos (mosXe) and the cyclin B component of maturation-promoting factor. Microinjection of Xenopus oocytes with in vitro-synthesized RNAs encoding Xenopus cyclin B1 or cyclin B2 induces the progression of meiosis, characterized by germinal vesicle breakdown (GVBD). By preinjecting oocytes with a mosXe-specific antisense oligonucleotide, we show that GVBD induced by cyclin B does not require expression of the mosXe protein. GVBD induced by cyclin B proceeds significantly faster than GVBD induced by progesterone or MosXe. However, coinjection of RNAs encoding cyclin B1 or cyclin B2 with mosXe RNA results in a 2.5- to 3-fold acceleration in GVBD relative to that induced by cyclin B alone. This acceleration of GVBD does not correlate with changes in the level of cyclin B1 and cyclin B2 phosphorylation.

journal_name

Mol Cell Biol

authors

Freeman RS,Ballantyne SM,Donoghue DJ

doi

10.1128/mcb.11.3.1713

subject

Has Abstract

pub_date

1991-03-01 00:00:00

pages

1713-7

issue

3

eissn

0270-7306

issn

1098-5549

journal_volume

11

pub_type

杂志文章
  • Two alternative pathways of double-strand break repair that are kinetically separable and independently modulated.

    abstract::HO endonuclease-induced double-strand breaks in Saccharomyces cerevisiae can undergo recombination by two distinct and competing pathways. In a plasmid containing a direct repeat, in which one repeat is interrupted by an HO endonuclease cut site, gap repair yields gene conversions while single-strand annealing produce...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.12.3.1292

    authors: Fishman-Lobell J,Rudin N,Haber JE

    更新日期:1992-03-01 00:00:00

  • Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation.

    abstract::Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1 (MBF1) was originally identified as a bridging molecule that connects the Drosophila nuclear receptor FTZ-F1 and TATA-bin...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.18.9.4971

    authors: Takemaru K,Harashima S,Ueda H,Hirose S

    更新日期:1998-09-01 00:00:00

  • A hierarchy of trans-acting factors modulates translation of an activator of amino acid biosynthetic genes in Saccharomyces cerevisiae.

    abstract::The GCN4 gene encodes a positive effector of amino acid biosynthetic genes in Saccharomyces cerevisiae. Genetic analysis has suggested that GCN4 is regulated by a hierarchy of interacting positive and negative effectors in response to amino acid starvation. Results presented here for a GCN4-lacZ gene fusion support th...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.5.9.2349

    authors: Hinnebusch AG

    更新日期:1985-09-01 00:00:00

  • Regulation of the protein kinase PKR by the vaccinia virus pseudosubstrate inhibitor K3L is dependent on residues conserved between the K3L protein and the PKR substrate eIF2alpha.

    abstract::The mammalian double-stranded RNA-activated protein kinase PKR is a component of the cellular antiviral defense mechanism and phosphorylates Ser-51 on the alpha subunit of the translation factor eIF2 to inhibit protein synthesis. To identify the molecular determinants that specify substrate recognition by PKR, we perf...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.17.7.4146

    authors: Kawagishi-Kobayashi M,Silverman JB,Ung TL,Dever TE

    更新日期:1997-07-01 00:00:00

  • DPH5, a methyltransferase gene required for diphthamide biosynthesis in Saccharomyces cerevisiae.

    abstract::A mutant of Saccharomyces cerevisiae defective in the S-adenosylmethionine (AdoMet)-dependent methyltransferase step of diphthamide biosynthesis was selected by intracellular expression of the F2 fragment of diphtheria toxin (DT) and shown to belong to complementation group DPH5. The DPH5 gene was cloned, sequenced, a...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.12.9.4026

    authors: Mattheakis LC,Shen WH,Collier RJ

    更新日期:1992-09-01 00:00:00

  • Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins.

    abstract::Microtubules in yeast are essential components of the mitotic and meiotic spindles and are essential for nuclear movement during cell division and mating. The relative importance in these processes of the two divergent alpha-tubulin genes of the budding yeast Saccharomyces cerevisiae, TUB1 and TUB3, was examined throu...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.6.11.3722

    authors: Schatz PJ,Solomon F,Botstein D

    更新日期:1986-11-01 00:00:00

  • Genes activated in the presence of an immunoglobulin enhancer or promoter are negatively regulated by a T-lymphoma cell line.

    abstract::The tissue-specific expression of immunoglobulin genes can be partially explained by a requirement for activating factors found only in B lymphocytes and their derivatives. However, loss of immunoglobulin expression upon fusion of an immunoglobulin-producing myeloma cell with a T lymphoma cell (BW5147) or fibroblast (...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.8.5.1932

    authors: Zaller DM,Yu H,Eckhardt LA

    更新日期:1988-05-01 00:00:00

  • p300 and PCAF act cooperatively to mediate transcriptional activation from chromatin templates by notch intracellular domains in vitro.

    abstract::Ligand activation of Notch receptors leads to release of the intracellular receptor domain (Notch IC), which translocates to the nucleus and interacts with the DNA-binding protein RBP-Jkappa to control expression of specific target genes. A number of proteins have been shown to interact with Notch ICs and to modulate ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.22.22.7812-7819.2002

    authors: Wallberg AE,Pedersen K,Lendahl U,Roeder RG

    更新日期:2002-11-01 00:00:00

  • DNA binding by MADS-box transcription factors: a molecular mechanism for differential DNA bending.

    abstract::The serum response factor (SRF) and myocyte enhancer factor 2A (MEF2A) represent two human members of the MADS-box transcription factor family. Each protein has a distinct biological function which is reflected by the distinct specificities of the proteins for coregulatory protein partners and DNA-binding sites. In th...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.17.5.2876

    authors: West AG,Shore P,Sharrocks AD

    更新日期:1997-05-01 00:00:00

  • NF-Y is essential for the recruitment of RNA polymerase II and inducible transcription of several CCAAT box-containing genes.

    abstract::Osteoclast differentiation factor (ODF)/receptor activator of NF-kappaB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the CCAAT box on the ODF promoter and regulates its basal transcriptional activity. The CCAAT box on the ODF gene is required...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.25.1.512-522.2005

    authors: Kabe Y,Yamada J,Uga H,Yamaguchi Y,Wada T,Handa H

    更新日期:2005-01-01 00:00:00

  • A role for c-myc in chemically induced renal-cell death.

    abstract::A variety of genes, including c-myc, are activated by chemical toxicants in vivo and in vitro. Although enforced c-myc expression induces apoptosis after withdrawing survival factors, it is not clear if activation of the endogenous c-myc gene is an apoptotic signal after toxicant exposure. The renal tubular epithelium...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.17.11.6755

    authors: Zhan Y,Cleveland JL,Stevens JL

    更新日期:1997-11-01 00:00:00

  • AMPK Promotes Aberrant PGC1β Expression To Support Human Colon Tumor Cell Survival.

    abstract::A major goal of cancer research is the identification of tumor-specific vulnerabilities that can be exploited for the development of therapies that are selectively toxic to the tumor. We show here that the transcriptional coactivators peroxisome proliferator-activated receptor gamma coactivator 1β (PGC1β) and estrogen...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00528-15

    authors: Fisher KW,Das B,Kim HS,Clymer BK,Gehring D,Smith DR,Costanzo-Garvey DL,Fernandez MR,Brattain MG,Kelly DL,MacMillan J,White MA,Lewis RE

    更新日期:2015-11-01 00:00:00

  • Functional cooperation between the proteins Nck and ADAP is fundamental for actin reorganization.

    abstract::T cell antigen receptor (TCR) activation triggers profound changes in the actin cytoskeleton. In addition to controlling cellular shape and polarity, this process regulates vital T cell responses, such as T cell adhesion, motility, and proliferation. These depend on the recruitment of the signaling proteins Nck and Wi...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01358-10

    authors: Pauker MH,Reicher B,Fried S,Perl O,Barda-Saad M

    更新日期:2011-07-01 00:00:00

  • Regulation of skeletal muscle sarcomere integrity and postnatal muscle function by Mef2c.

    abstract::Myocyte enhancer factor 2 (MEF2) transcription factors cooperate with the MyoD family of basic helix-loop-helix (bHLH) transcription factors to drive skeletal muscle development during embryogenesis, but little is known about the potential functions of MEF2 factors in postnatal skeletal muscle. Here we show that skele...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01187-07

    authors: Potthoff MJ,Arnold MA,McAnally J,Richardson JA,Bassel-Duby R,Olson EN

    更新日期:2007-12-01 00:00:00

  • A small nuclear RNA, U5, can transform cells in vitro.

    abstract::Low-molecular-weight RNA exhibiting transforming potential was identified in chemically induced lymphoma cells by the transformation of mink lung cells after transfection. The RNA was sequenced by the direct chemical method and was shown to be a small nuclear RNA, U5. The transforming potential of the RNA was further ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.9.10.4345

    authors: Hamada K,Kumazaki T,Mizuno K,Yokoro K

    更新日期:1989-10-01 00:00:00

  • Impaired retinoic acid (RA) signal leads to RARbeta2 epigenetic silencing and RA resistance.

    abstract::Resistance to the growth-inhibitory action of retinoic acid (RA), the bioactive derivative of vitamin A, is common in human tumors. One form of RA resistance has been associated with silencing and hypermethylation of the retinoic acid receptor beta2 gene (RARbeta2), an RA-regulated tumor suppressor gene. The presence ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.25.23.10591-10603.2005

    authors: Ren M,Pozzi S,Bistulfi G,Somenzi G,Rossetti S,Sacchi N

    更新日期:2005-12-01 00:00:00

  • Differential regulation of human interferon A gene expression by interferon regulatory factors 3 and 7.

    abstract::Differential expression of the human interferon A (IFN-A) gene cluster is modulated following paramyxovirus infection by the relative amounts of active interferon regulatory factor 3 (IRF-3) and IRF-7. IRF-3 expression activates predominantly IFN-A1 and IFN-B, while IRF-7 expression induces multiple IFN-A genes. IFN-A...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01805-08

    authors: Génin P,Lin R,Hiscott J,Civas A

    更新日期:2009-06-01 00:00:00

  • Structures and Functions of the Multiple KOW Domains of Transcription Elongation Factor Spt5.

    abstract::The eukaryotic Spt4-Spt5 heterodimer forms a higher-order complex with RNA polymerase II (and I) to regulate transcription elongation. Extensive genetic and functional data have revealed diverse roles of Spt4-Spt5 in coupling elongation with chromatin modification and RNA-processing pathways. A mechanistic understandi...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00520-15

    authors: Meyer PA,Li S,Zhang M,Yamada K,Takagi Y,Hartzog GA,Fu J

    更新日期:2015-10-01 00:00:00

  • RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination.

    abstract::The RAD1 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of damaged DNA. In this paper, we report our observations on the effect of the RAD1 gene on genetic recombination. Mitotic intrachromosomal and interchromosomal recombination in RAD+, rad1, rad52, and other rad mutant strain...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.8.9.3619

    authors: Schiestl RH,Prakash S

    更新日期:1988-09-01 00:00:00

  • The DIVa maturase binding site in the yeast group II intron aI2 is essential for intron homing but not for in vivo splicing.

    abstract::Splicing of the Saccharomyces cerevisiae mitochondrial DNA group II intron aI2 depends on the intron-encoded 62-kDa reverse transcriptase-maturase protein (p62). In wild-type strains, p62 remains associated with the excised intron lariat RNA in ribonucleoprotein (RNP) particles that are essential for intron homing. St...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.23.23.8809-8819.2003

    authors: Huang HR,Chao MY,Armstrong B,Wang Y,Lambowitz AM,Perlman PS

    更新日期:2003-12-01 00:00:00

  • Identification of a series of transforming growth factor beta-responsive genes by retrovirus-mediated gene trap screening.

    abstract::Transforming growth factor beta (TGF-beta) plays important roles in the regulation of proliferation, differentiation, apoptosis, and carcinogenesis. To identify genes responsible for maintaining the phenotype induced by TGF-beta, we performed a retrovirus-mediated gene trap screening designed to isolate TGF-beta-respo...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.20.9.3266-3273.2000

    authors: Akiyama N,Matsuo Y,Sai H,Noda M,Kizaka-Kondoh S

    更新日期:2000-05-01 00:00:00

  • Identification of a phospholipase C-gamma1 (PLC-gamma1) SH3 domain-binding site in SLP-76 required for T-cell receptor-mediated activation of PLC-gamma1 and NFAT.

    abstract::SLP-76 is an adapter protein required for T-cell receptor (TCR) signaling. In particular, TCR-induced tyrosine phosphorylation and activation of phospholipase C-gamma1 (PLC-gamma1), and the resultant TCR-inducible gene expression, depend on SLP-76. Nonetheless, the mechanisms by which SLP-76 mediates PLC-gamma1 activa...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.21.13.4208-4218.2001

    authors: Yablonski D,Kadlecek T,Weiss A

    更新日期:2001-07-01 00:00:00

  • Cellular functions of Ufd2 and Ufd3 in proteasomal protein degradation depend on Cdc48 binding.

    abstract::The chaperone-related AAA ATPase Cdc48 (p97/VCP in higher eukaryotes) segregates ubiquitylated proteins for subsequent degradation by the 26S proteasome or for nonproteolytic fates. The specific outcome of Cdc48 activity is controlled by the evolutionary conserved cofactors Ufd2 and Ufd3, which antagonistically regula...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00962-10

    authors: Böhm S,Lamberti G,Fernández-Sáiz V,Stapf C,Buchberger A

    更新日期:2011-04-01 00:00:00

  • TIN2 Functions with TPP1/POT1 To Stimulate Telomerase Processivity.

    abstract::TIN2 is an important regulator of telomere length, and mutations in TINF2, the gene encoding TIN2, cause short-telomere syndromes. While the genetics underscore the importance of TIN2, the mechanism through which TIN2 regulates telomere length remains unclear. Here, we tested the effects of human TIN2 on telomerase ac...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00593-18

    authors: Pike AM,Strong MA,Ouyang JPT,Greider CW

    更新日期:2019-10-11 00:00:00

  • trans Activation of the simian virus 40 enhancer.

    abstract::We describe experiments which demonstrated that the simian virus 40 (SV40) enhancer affects certain transcriptional units differently. We also found that a specific enhancer-transcriptional unit interaction can be regulated by trans-acting factors. Using transient assays, we examined the effects of the SV40 enhancer o...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.6.4.1283

    authors: Robbins PD,Rio DC,Botchan MR

    更新日期:1986-04-01 00:00:00

  • Ki-67 Contributes to Normal Cell Cycle Progression and Inactive X Heterochromatin in p21 Checkpoint-Proficient Human Cells.

    abstract::The Ki-67 protein is widely used as a tumor proliferation marker. However, whether Ki-67 affects cell cycle progression has been controversial. Here we demonstrate that depletion of Ki-67 in human hTERT-RPE1, WI-38, IMR90, and hTERT-BJ cell lines and primary fibroblast cells slowed entry into S phase and coordinately ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00569-16

    authors: Sun X,Bizhanova A,Matheson TD,Yu J,Zhu LJ,Kaufman PD

    更新日期:2017-08-11 00:00:00

  • Regulation of the transcription factor EB-PGC1α axis by beclin-1 controls mitochondrial quality and cardiomyocyte death under stress.

    abstract::In cardiac ischemia-reperfusion injury, reactive oxygen species (ROS) generation and upregulation of the hypoxia-inducible protein BNIP3 result in mitochondrial permeabilization, but impairment in autophagic removal of damaged mitochondria provokes programmed cardiomyocyte death. BNIP3 expression and ROS generation re...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01091-14

    authors: Ma X,Liu H,Murphy JT,Foyil SR,Godar RJ,Abuirqeba H,Weinheimer CJ,Barger PM,Diwan A

    更新日期:2015-03-01 00:00:00

  • Central role for the XRCC1 BRCT I domain in mammalian DNA single-strand break repair.

    abstract::The DNA single-strand break repair (SSBR) protein XRCC1 is required for genetic stability and for embryonic viability. XRCC1 possesses two BRCA1 carboxyl-terminal (BRCT) protein interaction domains, denoted BRCT I and II. BRCT II is required for SSBR during G(1) but is dispensable for this process during S/G(2) and co...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.22.8.2556-2563.2002

    authors: Taylor RM,Thistlethwaite A,Caldecott KW

    更新日期:2002-04-01 00:00:00

  • Coordination of immunoglobulin DJH transcription and D-to-JH rearrangement by promoter-enhancer approximation.

    abstract::The genes that encode the variable regions of immunoglobulin (Ig) heavy chains are encoded by three DNA segments: VH, D, and JH. During B-cell development these segments are brought together by a pair of site-specific DNA rearrangements. The first of these joins a D segment to a JH segment; the second brings a VH segm...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.11.4.2096

    authors: Alessandrini A,Desiderio SV

    更新日期:1991-04-01 00:00:00

  • The immunoglobulin heavy-chain enhancer functions as the promoter for I mu sterile transcription.

    abstract::We demonstrate that the immunoglobulin heavy-chain enhancer functions as the promoter for I mu sterile transcription. The enhancer itself, when placed 5' to the bacterial cat or neo genes, is able to direct transcription by using heterogeneous start sites that are generally the same as those found with bona fide I mu ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.10.6.2619

    authors: Su LK,Kadesch T

    更新日期:1990-06-01 00:00:00