Synergistic interactions between heterologous upstream activation elements and specific TATA sequences in a muscle-specific promoter.

Abstract:

:Previous investigations have defined three upstream activation elements--CCAC, A/T, and TATA sequences--necessary for muscle-specific transcription of the myoglobin gene. In the present study, we demonstrate that these three sequences elements, prepared as synthetic oligonucleotide cassettes, function synergistically to constitute a cell-type-specific transcription unit. Previously, cognate binding factors that recognize the CCAC and TATA elements were identified. In this study we determine that the A/T element binds two nuclear factors, including myocyte enhancer factor-2 (MEF-2) and an apparently unknown factor we provisionally termed ATF35 (A/T-binding factor, 35 kDa). Mutations that alter in vitro binding of either MEF-2 or ATF35 to this site diminish promoter function in vivo. Functional synergism between factors binding the CCAC and A/T elements is sensitive to subtle mutations in the TATA sequence, recapitulating the unusual preference for specific TATA variants exhibited by the native myoglobin promoter. These results provide new insights into mechanisms that underlie the distinctive pattern of myoglobin gene regulation in mammalian muscle development and lay a foundation for further studies to elucidate general principles of transcriptional control of complex mammalian promoters through combinatorial actions of heterologous transcriptional factors.

journal_name

Mol Cell Biol

authors

Grayson J,Williams RS,Yu YT,Bassel-Duby R

doi

10.1128/mcb.15.4.1870

subject

Has Abstract

pub_date

1995-04-01 00:00:00

pages

1870-8

issue

4

eissn

0270-7306

issn

1098-5549

journal_volume

15

pub_type

杂志文章
  • Evidence for high-capacity bidirectional glucose transport across the endoplasmic reticulum membrane by genetically encoded fluorescence resonance energy transfer nanosensors.

    abstract::Glucose release from hepatocytes is important for maintenance of blood glucose levels. Glucose-6-phosphate phosphatase, catalyzing the final metabolic step of gluconeogenesis, faces the endoplasmic reticulum (ER) lumen. Thus, glucose produced in the ER has to be either exported from the ER into the cytosol before rele...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.25.24.11102-11112.2005

    authors: Fehr M,Takanaga H,Ehrhardt DW,Frommer WB

    更新日期:2005-12-01 00:00:00

  • DPH5, a methyltransferase gene required for diphthamide biosynthesis in Saccharomyces cerevisiae.

    abstract::A mutant of Saccharomyces cerevisiae defective in the S-adenosylmethionine (AdoMet)-dependent methyltransferase step of diphthamide biosynthesis was selected by intracellular expression of the F2 fragment of diphtheria toxin (DT) and shown to belong to complementation group DPH5. The DPH5 gene was cloned, sequenced, a...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.12.9.4026

    authors: Mattheakis LC,Shen WH,Collier RJ

    更新日期:1992-09-01 00:00:00

  • A regulatory role for microRNA 33* in controlling lipid metabolism gene expression.

    abstract::hsa-miR-33a and hsa-miR-33b, intronic microRNAs (miRNAs) located within the sterol regulatory element-binding protein 2 and 1 genes (Srebp-2 and -1), respectively, have recently been shown to regulate lipid homeostasis in concert with their host genes. Although the functional role of miR-33a and -b has been highly inv...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01714-12

    authors: Goedeke L,Vales-Lara FM,Fenstermaker M,Cirera-Salinas D,Chamorro-Jorganes A,Ramírez CM,Mattison JA,de Cabo R,Suárez Y,Fernández-Hernando C

    更新日期:2013-06-01 00:00:00

  • Interdependent interactions between TFIIB, TATA binding protein, and DNA.

    abstract::Temperature-sensitive mutants of TFIIB that are defective for essential interactions were isolated. One mutation (G204D) results in disruption of a protein-protein contact between TFIIB and TATA binding protein (TBP), while the other (K272I) disrupts an interaction between TFIIB and DNA. The TBP gene was mutagenized, ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.22.24.8735-8743.2002

    authors: Buratowski RM,Downs J,Buratowski S

    更新日期:2002-12-01 00:00:00

  • Effects of the SANT domain of tension-induced/inhibited proteins (TIPs), novel partners of the histone acetyltransferase p300, on p300 activity and TIP-6-induced adipogenesis.

    abstract::We previously identified a set of transcription regulators, referred to as TIPs (tension-induced/inhibited proteins), with a role in myogenic versus adipogenic differentiation. Here we report that the TIP family comprises eight isoforms, all bearing a SANT (switching-defective protein 3, adaptor 2, nuclear receptor co...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00333-08

    authors: Badri KR,Zhou Y,Dhru U,Aramgam S,Schuger L

    更新日期:2008-10-01 00:00:00

  • Expression of chimeric tRNA-driven antisense transcripts renders NIH 3T3 cells highly resistant to Moloney murine leukemia virus replication.

    abstract::NIH 3T3 cells infected with Moloney murine leukemia virus (MoMLV) express high levels of virus-specific RNA. To inhibit replication of the virus, we stably introduced chimeric tRNA genes encoding antisense templates into NIH 3T3 cells via a retroviral vector. Efficient expression of hybrid tRNA-MoMLV antisense transcr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.10.12.6512

    authors: Sullenger BA,Lee TC,Smith CA,Ungers GE,Gilboa E

    更新日期:1990-12-01 00:00:00

  • Rearrangement of rat immunoglobulin E heavy-chain and c-myc genes in the B-cell immunocytoma IR162.

    abstract::Mammalian B-cell lymphoid malignancies frequently display aberrant translocations involving the c-myc proto-oncogene and one of the immunoglobulin loci. We have observed and characterized such a translocation in the immunoglobulin E-producing rat immunocytoma IR162 by using recombinant DNA technology. We show here for...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.7.7.2614

    authors: Tian SS,Faust C

    更新日期:1987-07-01 00:00:00

  • A mutation allowing an mRNA secondary structure diminishes translation of Saccharomyces cerevisiae iso-1-cytochrome c.

    abstract::The CYC1-239-O mutation in the yeast Saccharomyces cerevisiae produces a -His-Leu- replacement of the normal -Ala-Gly- sequence at amino acid positions 5 and 6, which lie within a dispensable region of iso-1-cytochrome c; this mutation can accommodate the formation of a hairpin structure at the corresponding site in t...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.5.8.1839

    authors: Baim SB,Pietras DF,Eustice DC,Sherman F

    更新日期:1985-08-01 00:00:00

  • c-myc Internal ribosome entry site activity is developmentally controlled and subjected to a strong translational repression in adult transgenic mice.

    abstract::The expression of c-myc proto-oncogene, a key regulator of cell proliferation and apoptosis, is controlled at different transcriptional and posttranscriptional levels. In particular, the c-myc mRNA contains an internal ribosome entry site (IRES) able to promote translation initiation independently from the classical c...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.21.5.1833-1840.2001

    authors: Créancier L,Mercier P,Prats AC,Morello D

    更新日期:2001-03-01 00:00:00

  • The Isw2 chromatin-remodeling ATPase cooperates with the Fkh2 transcription factor to repress transcription of the B-type cyclin gene CLB2.

    abstract::Forkhead (Fkh) transcription factors influence cell death, proliferation, and differentiation and the cell cycle. In Saccharomyces cerevisiae, Fkh2 both activates and represses transcription of CLB2, encoding a B-type cyclin. CLB2 is expressed during G(2)/M phase and repressed during G(1). Fkh2 recruits the coactivato...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01798-06

    authors: Sherriff JA,Kent NA,Mellor J

    更新日期:2007-04-01 00:00:00

  • Biochemical and functional analyses of a secreted H-2Ld molecule.

    abstract::A truncated H-2Ld gene was constructed by deleting the transmembrane and cytoplasmic exons. The truncated H-2Ld gene was introduced into mouse L cells using the thymidine kinase gene as a selectable marker. Transformants were isolated and screened for the presence of truncated H-2Ld antigen. The truncated H-2Ld gene p...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.6.4.1315

    authors: Murre C,Parker KC,Reiss CS,Driller JP,Wiley DC,Burakoff SJ,Seidman JG

    更新日期:1986-04-01 00:00:00

  • A hierarchical network of interreceptor interactions determines signal transduction by Neu differentiation factor/neuregulin and epidermal growth factor.

    abstract::The ErbB family includes four homologous transmembrane tyrosine kinases. Whereas ErbB-1 binds to the epidermal growth factor (EGF), both ErbB-3 and ErbB-4 bind to the Neu differentiation factors (NDFs, or neuregulins), and ErbB-2, the most oncogenic family member, is an orphan receptor whose function is still unknown....

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.16.10.5276

    authors: Tzahar E,Waterman H,Chen X,Levkowitz G,Karunagaran D,Lavi S,Ratzkin BJ,Yarden Y

    更新日期:1996-10-01 00:00:00

  • Immune functions in mice lacking Clnk, an SLP-76-related adaptor expressed in a subset of immune cells.

    abstract::The SLP-76 family of immune cell-specific adaptors is composed of three distinct members named SLP-76, Blnk, and Clnk. They have been implicated in the signaling pathways coupled to immunoreceptors such as the antigen receptors and Fc receptors. Previous studies using gene-targeted mice and deficient cell lines showed...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.24.13.6067-6075.2004

    authors: Utting O,Sedgmen BJ,Watts TH,Shi X,Rottapel R,Iulianella A,Lohnes D,Veillette A

    更新日期:2004-07-01 00:00:00

  • Expression of the glycoprotein hormone alpha-subunit gene in the placenta requires a functional cyclic AMP response element, whereas a different cis-acting element mediates pituitary-specific expression.

    abstract::The single-copy gene encoding the alpha subunit of glycoprotein hormones is expressed in the pituitaries of all mammals and in the placentas of only primates and horses. We have systematically analyzed the promoter-regulatory elements of the human and bovine alpha-subunit genes to elucidate the molecular mechanisms un...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.9.11.5113

    authors: Bokar JA,Keri RA,Farmerie TA,Fenstermaker RA,Andersen B,Hamernik DL,Yun J,Wagner T,Nilson JH

    更新日期:1989-11-01 00:00:00

  • A ligand binding domain mutation in the mouse glucocorticoid receptor functionally links chromatin remodeling and transcription initiation.

    abstract::We utilized the mouse mammary tumor virus (MMTV) long terminal repeat (LTR) in vivo to understand how the interaction of the glucocorticoid receptor (GR) with a nucleosome-assembled promoter allows access of factors required for the transition from a repressed promoter to a derepressed, transcriptionally competent pro...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.19.12.8146

    authors: Sheldon LA,Smith CL,Bodwell JE,Munck AU,Hager GL

    更新日期:1999-12-01 00:00:00

  • Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1.

    abstract::Synonymous single nucleotide polymorphisms (SNPs) within a transcript's coding region produce no change in the amino acid sequence of the protein product and are therefore intuitively assumed to have a neutral effect on protein function. We report that two common variants of high-temperature requirement A1 (HTRA1) tha...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01283-12

    authors: Jacobo SM,Deangelis MM,Kim IK,Kazlauskas A

    更新日期:2013-05-01 00:00:00

  • Inheritance of extrachromosomal rDNA in Physarum polycephalum.

    abstract::In the acellular slime mold Physarum polycephalum, the several hundred genes coding for rRNA are located on linear extrachromosomal DNA molecules of a discrete size, 60 kilobases. Each molecule contains two genes that are arranged in a palindromic fashion and separated by a central spacer region. We investigated how r...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.3.4.635

    authors: Ferris PJ,Vogt VM,Truitt CL

    更新日期:1983-04-01 00:00:00

  • HOXA9 participates in the transcriptional activation of E-selectin in endothelial cells.

    abstract::The homeobox gene HOXA9 has recently been shown to be an important regulator of endothelial cell (EC) differentiation and activation in addition to its role in embryonic development and hematopoiesis. In this report, we have determined that the EC-leukocyte adhesion molecule E-selectin is a key target for HOXA9. The d...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00052-07

    authors: Bandyopadhyay S,Ashraf MZ,Daher P,Howe PH,DiCorleto PE

    更新日期:2007-06-01 00:00:00

  • Cellular Moloney murine sarcoma (c-mos) sequences are hypermethylated and transcriptionally silent in normal and transformed rodent cells.

    abstract::Moloney murine sarcoma virus carries an oncogenic sequence (v-mos) which is homologous to a single copy gene (c-mos) present in the normal cells of several vertebrate species. Because of the possible significance of c-mos sequences in normal development and malignant transformation induced by physical or chemical agen...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.2.1.42

    authors: Gattoni S,Kirschmeier P,Weinstein IB,Escobedo J,Dina D

    更新日期:1982-01-01 00:00:00

  • A Novel Regulatory Axis, CHD1L-MicroRNA 486-Matrix Metalloproteinase 2, Controls Spermatogonial Stem Cell Properties.

    abstract::Spermatogonial stem cells (SSCs) are unipotent germ cells that are at the foundation of spermatogenesis and male fertility. However, the underlying molecular mechanisms governing SSC stemness and growth properties remain elusive. We have recently identified chromodomain helicase/ATPase DNA binding protein 1-like (Chd1...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00357-18

    authors: Liu SS,Maguire EM,Bai YS,Huang L,Liu Y,Xu L,Fauzi I,Zhang SQ,Xiao Q,Ma NF

    更新日期:2019-02-04 00:00:00

  • ClipR-59 interacts with Akt and regulates Akt cellular compartmentalization.

    abstract::Akt is activated on the plasma membrane and its substrates are distributed throughout various cellular compartments. To phosphorylate its substrates, Akt needs to be recruited to specific intracellular compartments. Thus, regulation of Akt cellular compartmentalization constitutes an important mechanism to specify Akt...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00754-08

    authors: Ding J,Du K

    更新日期:2009-03-01 00:00:00

  • Compartmentalized Ras proteins transform NIH 3T3 cells with different efficiencies.

    abstract::Ras GTPases were long thought to function exclusively from the plasma membrane (PM). However, a current model suggests that Ras proteins can compartmentalize to regulate different functions, and an oncogenic H-Ras mutant that is restricted to the endomembrane can still transform cells. In this study, we demonstrated t...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00137-10

    authors: Cheng CM,Li H,Gasman S,Huang J,Schiff R,Chang EC

    更新日期:2011-03-01 00:00:00

  • Essential role of insulin receptor substrate 1 (IRS-1) and IRS-2 in adipocyte differentiation.

    abstract::To investigate the role of insulin receptor substrate 1 (IRS-1) and IRS-2, the two ubiquitously expressed IRS proteins, in adipocyte differentiation, we established embryonic fibroblast cells with four different genotypes, i.e., wild-type, IRS-1 deficient (IRS-1(-/-)), IRS-2 deficient (IRS-2(-/-)), and IRS-1 IRS-2 dou...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.21.7.2521-2532.2001

    authors: Miki H,Yamauchi T,Suzuki R,Komeda K,Tsuchida A,Kubota N,Terauchi Y,Kamon J,Kaburagi Y,Matsui J,Akanuma Y,Nagai R,Kimura S,Tobe K,Kadowaki T

    更新日期:2001-04-01 00:00:00

  • Core binding factor cannot synergistically activate the myeloperoxidase proximal enhancer in immature myeloid cells without c-Myb.

    abstract::The myeloperoxidase (MPO) gene is transcribed specifically in immature myeloid cells and is regulated in part by a 414-bp proximal enhancer. Mutation of a core binding factor (CBF)-binding site at -288 decreased enhancer activity 30-fold in 32D cl3 myeloid cells cultured in granulocyte colony-stimulating factor (G-CSF...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.17.9.5127

    authors: Britos-Bray M,Friedman AD

    更新日期:1997-09-01 00:00:00

  • DNA-binding activities of the Drosophila melanogaster even-skipped protein are mediated by its homeo domain and influenced by protein context.

    abstract::The homeo box gene even-skipped (eve) encodes a 376-amino-acid protein that binds with high affinity to sequences located near the 5' termini of the eve and en genes. The 5' en sites are A + T rich and contain copies of the 10-base-pair (bp) consensus sequence T-C-A-A-T-T-A-A-A-T. In contrast, the 5' eve sites are G +...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.8.11.4598

    authors: Hoey T,Warrior R,Manak J,Levine M

    更新日期:1988-11-01 00:00:00

  • Cloning and characterization of two novel thyroid hormone receptor beta isoforms.

    abstract::Thyroid hormone (T(3)) activates nuclear receptor transcription factors, encoded by the TRalpha (NR1A1) and TRbeta (NR1A2) genes, to regulate target gene expression. Several TR isoforms exist, and studies of null mice have identified some unique functions for individual TR variants, although considerable redundancy oc...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.20.22.8329-8342.2000

    authors: Williams GR

    更新日期:2000-11-01 00:00:00

  • Synergy between Apc min and an activated ras mutation is sufficient to induce colon carcinomas.

    abstract::Colon carcinomas appear to arise from the cumulative effect of mutations to several genes (APC, DCC, p53, ras, hMLH1, and hMSH2). By using novel colonic epithelial cell lines derived from the Immorto mouse, named the YAMC (young adult mouse colon) cell line, and an Immorto-Min mouse hybrid, named the IMCE (Immorto-Min...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.16.3.884

    authors: D'Abaco GM,Whitehead RH,Burgess AW

    更新日期:1996-03-01 00:00:00

  • The cellular response to neuregulins is governed by complex interactions of the erbB receptor family.

    abstract::Deregulated signaling by the four members of the epidermal growth factor receptor tyrosine kinase family (erbB family) is implicated in the genesis or progression of human cancers. However, efforts to analyze signaling by these receptors have been hampered by the diversity of ligands and extensive interreceptor cross ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.15.10.5770

    authors: Riese DJ 2nd,van Raaij TM,Plowman GD,Andrews GC,Stern DF

    更新日期:1995-10-01 00:00:00

  • Rac1 activation in podocytes induces rapid foot process effacement and proteinuria.

    abstract::The kidney's vital filtration function depends on the structural integrity of the glomerulus, the proximal portion of the nephron. Within the glomerulus, the architecturally complex podocyte forms the final cellular barrier to filtration. Injury to the podocyte results in a morphological change called foot process eff...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00730-13

    authors: Yu H,Suleiman H,Kim AH,Miner JH,Dani A,Shaw AS,Akilesh S

    更新日期:2013-12-01 00:00:00

  • Regulation of the protein kinase PKR by the vaccinia virus pseudosubstrate inhibitor K3L is dependent on residues conserved between the K3L protein and the PKR substrate eIF2alpha.

    abstract::The mammalian double-stranded RNA-activated protein kinase PKR is a component of the cellular antiviral defense mechanism and phosphorylates Ser-51 on the alpha subunit of the translation factor eIF2 to inhibit protein synthesis. To identify the molecular determinants that specify substrate recognition by PKR, we perf...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.17.7.4146

    authors: Kawagishi-Kobayashi M,Silverman JB,Ung TL,Dever TE

    更新日期:1997-07-01 00:00:00