A complex androgen-responsive enhancer resides 2 kilobases upstream of the mouse Slp gene.

abstract：:The Saccharomyces cerevisiae SWI4 gene encodes an essential transcription factor which controls gene expression at the G1/S transition of the cell cycle. SWI4 transcription itself is cell cycle regulated, and this periodicity is crucial for the normal cell cycle regulation of HO and at least two of the G1 cyclins. Sin...

journal_title：Molecular and cellular biology

authors： Foster R,Mikesell GE,Breeden L

更新日期：1993-06-01 00:00:00

abstract：:B-lymphocyte development requires the basic helix-loop-helix proteins encoded by the E2A gene. In this study, the control mechanism of E2A was further explored by disruption of the E2A-related genes, E2-2 and HEB. In contrast to E2A, E2-2 and HEB are not essential for the establishment of the B-cell lineage. However, ...

journal_title：Molecular and cellular biology

authors： Zhuang Y,Cheng P,Weintraub H

更新日期：1996-06-01 00:00:00

abstract：:The specific (6;9)(p23;q34) chromosomal translocation is associated with a defined subtype of acute nonlymphocytic leukemia (ANLL). The 9q34 breakpoint is located at the telomeric side of the c-abl gene. Through a combination of chromosome jumping, long-range mapping, and chromosome walking, the chromosome 9 breakpoin...

journal_title：Molecular and cellular biology

authors： von Lindern M,Poustka A,Lerach H,Grosveld G

更新日期：1990-08-01 00:00:00

abstract：:mRNAs are monitored for errors in gene expression by RNA surveillance, in which mRNAs that cannot be fully translated are degraded by the nonsense-mediated mRNA decay pathway (NMD). RNA surveillance ensures that potentially deleterious truncated proteins are seldom made. NMD pathways that promote surveillance have bee...

journal_title：Molecular and cellular biology

authors： Lelivelt MJ,Culbertson MR

更新日期：1999-10-01 00:00:00

abstract：:The mechanism by which the maize autonomous Ac transposable element gives rise to nonautonomous Ds elements is largely unknown. Sequence analysis of native maize Ds elements indicates a complex chimeric structure formed through deletions of Ac sequences with or without insertions of Ac-unrelated sequence blocks. These...

journal_title：Molecular and cellular biology

authors： Rubin E,Levy AA

更新日期：1997-11-01 00:00:00

abstract：:Although the U1 small nuclear ribonucleoprotein particle (snRNP) was the first mRNA-splicing cofactor to be identified, the manner in which it functions in splicing is not precisely understood. Among the information required to understand how U1 snRNP participates in splicing, it will be necessary to know its structur...

journal_title：Molecular and cellular biology

authors： Patton JR,Patterson RJ,Pederson T

更新日期：1987-11-01 00:00:00

abstract：:We assayed phosphatidylinositol (PI) kinase (EC 2.7.1.67) activity in detergent extracts of nontransformed or virus-transformed cells. Nontransformed chicken embryo fibroblasts (CEF) contain PI kinase activity with an apparent specific activity of 20 pmol/min per mg of protein. This activity sedimented as a single pea...

journal_title：Molecular and cellular biology

authors： Sugano S,Hanafusa H

更新日期：1985-09-01 00:00:00

abstract：:Although the splicing of transcripts from most eukaryotic genes occurs in a constitutive fashion, some genes can undergo a process of alternative splicing. This is a genetically economical process which allows a single gene to give rise to several protein isoforms by the inclusion or exclusion of sequences into or fro...

journal_title：Molecular and cellular biology

authors： Bell MV,Cowper AE,Lefranc MP,Bell JI,Screaton GR

更新日期：1998-10-01 00:00:00

abstract：:Saccharomyces mating-type switching occurs through a double-strand break-initiated gene conversion event at MAT, using one of two donors located distantly on the same chromosome, HMLalpha and HMRa. MATa cells preferentially choose HMLalpha, a decision that depends on the recombination enhancer (RE) that controls recom...

journal_title：Molecular and cellular biology

authors： Coïc E,Sun K,Wu C,Haber JE

更新日期：2006-07-01 00:00:00

abstract：:Transcriptional regulation by nuclear receptors is controlled by the concerted action of coactivator and corepressor proteins. The product of the thyroid hormone-regulated mammalian gene hairless (Hr) was recently shown to function as a thyroid hormone receptor corepressor. Here we report that Hr acts as a potent repr...

journal_title：Molecular and cellular biology

authors： Moraitis AN,Giguère V,Thompson CC

更新日期：2002-10-01 00:00:00

abstract：:Combined deficiencies of poly(ADP)ribosyl polymerase 1 (PARP1) and ataxia telangiectasia mutated (ATM) result in synthetic lethality and, in the mouse, early embryonic death. Here, we investigated the genetic requirements for this lethality via analysis of mice deficient for PARP1 and either of two ATM-regulated DNA d...

journal_title：Molecular and cellular biology

authors： Orsburn B,Escudero B,Prakash M,Gesheva S,Liu G,Huso DL,Franco S

更新日期：2010-05-01 00:00:00

abstract：:We have characterized two P-element-induced, lethal mutations in Drosophila melanogaster which affect the larval hemocytes, mediators of the insect immune response. Each mutant displays larval melanotic tumors characteristic of mutations affecting the insect cellular immune system, and the moribund animals develop gro...

journal_title：Molecular and cellular biology

authors： Stewart MJ,Denell R

更新日期：1993-04-01 00:00:00

abstract：:The Ace2p and Swi5p zinc finger proteins have nearly identical DNA-binding domains, yet in vivo they activate transcription of different genes, CTS1 and HO. We now demonstrate that Ace2p and Swi5p recognize sites in the CTS1 and HO promoters with the same affinities, raising the question of how promoter specificity is...

journal_title：Molecular and cellular biology

authors： Dohrmann PR,Voth WP,Stillman DJ

更新日期：1996-04-01 00:00:00

abstract：:We isolated the gene for citrate synthase (citrate oxaloacetate lyase; EC 4.1.3.7) from Saccharomyces cerevisiae and ablated it by inserting the yeast LEU2 gene within its reading frame. This revealed a second, nonmitochondrial citrate synthase. Like the mitochondrial enzyme, this enzyme was sensitive to glucose repre...

journal_title：Molecular and cellular biology

authors： Rickey TM,Lewin AS

更新日期：1986-02-01 00:00:00

abstract：:Double-stranded (ds) RNA-specific adenosine deaminase converts adenosine residues into inosines in dsRNA and edits transcripts of certain cellular and viral genes such as glutamate receptor (GluR) subunits and hepatitis delta antigen. The first member of this type of deaminase, DRADA1, has been recently cloned based o...

journal_title：Molecular and cellular biology

authors： Lai F,Chen CX,Carter KC,Nishikura K

更新日期：1997-05-01 00:00:00

abstract：:We have constructed a size variant of the Drosophila hsp28 gene by deleting 207 base pairs of the protein coding region, beginning 33 base pairs downstream of the ATG protein initiation codon. After transformation of Drosophila melanogaster rosy (ry506) flies with this altered gene, using the P transposable element sy...

journal_title：Molecular and cellular biology

authors： Hoffman EP,Corces VG

更新日期：1984-12-01 00:00:00

abstract：:We have cloned CDC9, the structural gene for Saccharomyces cerevisiae DNA ligase, and investigated its transcriptional regulation both as a function of cell cycle stage and after UV irradiation. The steady-state level of DNA ligase mRNA increases at least fourfold in late G1, after the completion of start but before S...

journal_title：Molecular and cellular biology

authors： Peterson TA,Prakash L,Prakash S,Osley MA,Reed SI

更新日期：1985-01-01 00:00:00

abstract：:Using nuclear runoff transcription assays we demonstrated that alpha interferon-mediated induction of transcription of four mRNAs in HeLa monolayer cells needed ongoing protein synthesis and that such a need could be obviated by pretreating the cells with gamma interferon which, by itself, did not induce transcription...

journal_title：Molecular and cellular biology

authors： Kusari J,Sen GC

更新日期：1987-01-01 00:00:00

abstract：:Determining the functions of novel genes implicated in cell survival is directly relevant to our understanding of mammalian development and carcinogenesis. ARS2 is an evolutionarily conserved gene that confers arsenite resistance on arsenite-sensitive Chinese hamster ovary cells. Little is known regarding the function...

journal_title：Molecular and cellular biology

authors： Wilson MD,Wang D,Wagner R,Breyssens H,Gertsenstein M,Lobe C,Lu X,Nagy A,Burke RD,Koop BF,Howard PL

更新日期：2008-03-01 00:00:00

abstract：:Gamma interferon activation factor (GAF) rapidly induces transcriptional activation of gamma interferon (IFN-gamma)-responsive genes. Conversion of the GAF from a latent cytoplasmic to an activated, DNA-binding form is an immediate step in the cellular response to IFN-gamma. The amount of IFN-gamma-activated GAF, meas...

journal_title：Molecular and cellular biology

authors： Eilers A,Seegert D,Schindler C,Baccarini M,Decker T

更新日期：1993-06-01 00:00:00

abstract：:We analyzed the binding site(s) for Grb2 on the epidermal growth factor (EGF) receptor (EGFR), using cell lines overexpressing EGFRs containing various point and deletion mutations in the carboxy-terminal tail. Results of co-immunoprecipitation experiments suggest that phosphotyrosines Y-1068 and Y-1173 mediate the bi...

journal_title：Molecular and cellular biology

authors： Batzer AG,Rotin D,Ureña JM,Skolnik EY,Schlessinger J

更新日期：1994-08-01 00:00:00

abstract：:Thyroid hormone receptors are encoded by the TRalpha (NR1A1) and TRbeta (NR1A2) loci. These genes are transcribed into multiple variants whose functions are unclear. Analysis by gene inactivation in mice has provided new insights into the functional complexity of these products. Different strategies designed to modify...

journal_title：Molecular and cellular biology

authors： Gauthier K,Plateroti M,Harvey CB,Williams GR,Weiss RE,Refetoff S,Willott JF,Sundin V,Roux JP,Malaval L,Hara M,Samarut J,Chassande O

更新日期：2001-07-01 00:00:00

abstract：:Protein phosphatase 2A (PP2A) is a multimeric serine/threonine phosphatase that carries out multiple functions. Although numerous observations suggest that PP2A plays a major role in downregulation of the mitogen-activated protein (MAP) kinase pathway, the precise mechanisms are unknown. To clarify the role of PP2A in...

journal_title：Molecular and cellular biology

authors： Ugi S,Imamura T,Ricketts W,Olefsky JM

更新日期：2002-04-01 00:00:00

abstract：:RNA-dependent RNA polymerase (RdRP) plays key roles in RNA silencing to generate double-stranded RNAs. In model organisms, such as Caenorhabditis elegans and Neurospora crassa, two types of small interfering RNAs (siRNAs), primary siRNAs and secondary siRNAs, are expressed; RdRP produces secondary siRNAs de novo, with...

journal_title：Molecular and cellular biology

authors： Maida Y,Yasukawa M,Masutomi K

更新日期：2016-03-31 00:00:00

abstract：:SWI-SNF is an ATP-dependent chromatin remodeling complex that disrupts DNA-histone interactions. Several studies of SWI-SNF activity on mononucleosome substrates have suggested that remodeling leads to novel, accessible nucleosomes which persist in the absence of continuous ATP hydrolysis. In contrast, we have reporte...

journal_title：Molecular and cellular biology

authors： Jaskelioff M,Gavin IM,Peterson CL,Logie C

更新日期：2000-05-01 00:00:00

abstract：:Caveolae are specialized microdomains on membranes that are critical for signal transduction, cholesterol transport, and endocytosis. Caveolin-1 (CAV1) is a multifunctional protein and a major component of caveolae. Cav1 is directly activated by hypoxia-inducible factor (HIF). HIFs are heterodimers of an oxygen-sensit...

journal_title：Molecular and cellular biology

authors： Xie L,Xue X,Taylor M,Ramakrishnan SK,Nagaoka K,Hao C,Gonzalez FJ,Shah YM

更新日期：2014-08-01 00:00:00

abstract：:It is thought that degradation of nuclear proteins by the ubiquitylation system requires nuclear-cytoplasmic trafficking of E3 ubiquitin ligases. The von Hippel-Lindau (VHL) tumor suppressor protein is the substrate recognition component of a Cullin-2-containing E3 ubiquitin ligase that recruits hypoxia-inducible fact...

journal_title：Molecular and cellular biology

authors： Khacho M,Mekhail K,Pilon-Larose K,Payette J,Lee S

更新日期：2008-01-01 00:00:00

abstract：:Previously, we identified the E3 ubiquitin ligase Idol (inducible degrader of the low-density lipoprotein [LDL] receptor [LDLR]) as a posttranscriptional regulator of the LDLR pathway. Idol stimulates LDLR degradation through ubiquitination of its C-terminal domain, thereby limiting cholesterol uptake. Here we report ...

journal_title：Molecular and cellular biology

authors： Scotti E,Hong C,Yoshinaga Y,Tu Y,Hu Y,Zelcer N,Boyadjian R,de Jong PJ,Young SG,Fong LG,Tontonoz P

更新日期：2011-05-01 00:00:00

abstract：:Heterochromatin protein 1 (HP1) is a conserved chromosomal protein with important roles in chromatin packaging and gene silencing. In fission yeast, two HP1 family proteins, Swi6 and Chp2, are involved in transcriptional silencing at heterochromatic regions, but how they function and whether they act cooperatively or ...

journal_title：Molecular and cellular biology

authors： Sadaie M,Kawaguchi R,Ohtani Y,Arisaka F,Tanaka K,Shirahige K,Nakayama J

更新日期：2008-12-01 00:00:00

abstract：:O(6)-Methylguanine (m6G) is formed by the action of alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on DNA. m6G is a highly mutagenic and carcinogenic lesion, and it presents a block to synthesis by DNA polymerases. Here, we provide genetic and biochemical evidence for the involvement of yeast an...

journal_title：Molecular and cellular biology

authors： Haracska L,Prakash S,Prakash L

更新日期：2000-11-01 00:00:00