Editing of glutamate receptor B subunit ion channel RNAs by four alternatively spliced DRADA2 double-stranded RNA adenosine deaminases.

abstract：:A transgenic gpt+ Chinese hamster cell line (G12) was found to be susceptible to carcinogenic nickel-induced inactivation of gpt expression without mutagenesis or deletion of the transgene. Many nickel-induced 6-thioguanine-resistant variants spontaneously reverted to actively express gpt, as indicated by both reversi...

journal_title：Molecular and cellular biology

authors： Lee YW,Klein CB,Kargacin B,Salnikow K,Kitahara J,Dowjat K,Zhitkovich A,Christie NT,Costa M

更新日期：1995-05-01 00:00:00

abstract：:When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority of HCF-1 in the cell is a complex of associated amino (HCF-1(N))- and carboxy (HCF-1(C))-terminal subunits that result fr...

journal_title：Molecular and cellular biology

authors： Wilson AC,Boutros M,Johnson KM,Herr W

更新日期：2000-09-01 00:00:00

abstract：:Neighboring genes encoding the mouse sex-limited protein (Slp) and fourth component of complement (C4) show extensive homology. In contrast to C4, however, Slp is regulated by androgen. One region of the Slp gene capable of hormonal response following transfection was located about 2 kilobases upstream of the transcri...

journal_title：Molecular and cellular biology

authors： Loreni F,Stavenhagen J,Kalff M,Robins DM

更新日期：1988-06-01 00:00:00

abstract：:The retinoblastoma protein (Rb) regulates both the cell cycle and tissue-specific transcription, by modulating the activity of factors that associate with its A-B and C pockets. In skeletal muscle, Rb has been reported to regulate irreversible cell cycle exit and muscle-specific transcription. To identify factors inte...

journal_title：Molecular and cellular biology

authors： MacLellan WR,Xiao G,Abdellatif M,Schneider MD

更新日期：2000-12-01 00:00:00

abstract：:Bone morphogenetic protein 7 (BMP7) stimulates renal branching morphogenesis via p38 mitogen-activated protein kinase (p38(MAPK)) and activating transcription factor 2 (ATF-2) (M. C. Hu, D. Wasserman, S. Hartwig, and N. D. Rosenblum, J. Biol. Chem. 279:12051-12059, 2004). Here, we demonstrate a novel role for integrin...

journal_title：Molecular and cellular biology

authors： Leung-Hagesteijn C,Hu MC,Mahendra AS,Hartwig S,Klamut HJ,Rosenblum ND,Hannigan GE

更新日期：2005-05-01 00:00:00

abstract：:Nuclear respiratory-defective mutants of Saccharomyces cerevisiae have been screened for lesions in the mitochondrial alpha-ketoglutarate dehydrogenase complex. Strains assigned to complementation group G70 were ascertained to be deficient in enzyme activity due to mutations in the KGD1 gene coding for the alpha-ketog...

journal_title：Molecular and cellular biology

authors： Repetto B,Tzagoloff A

更新日期：1989-06-01 00:00:00

abstract：:We analyzed the replication of two unlinked actin genes, ardB and ardC , which are abundantly transcribed in the naturally synchronous plasmodium of the slime mold Physarum polycephalum. Detection and size measurements of single-stranded nascent replication intermediates (RIs) demonstrate that these two genes are conc...

journal_title：Molecular and cellular biology

authors： Bénard M,Lagnel C,Pallotta D,Pierron G

更新日期：1996-03-01 00:00:00

abstract：:Transcription from the Moloney murine leukemia virus (Mo-MuLV) long terminal repeat (LTR) is inhibited in murine stem cells and induced during maturation of these cells. We have investigated whether alterations in the activity of this viral regulatory element also occur during differentiation of human myeloid leukemia...

journal_title：Molecular and cellular biology

authors： Reisman D,Rotter V

更新日期：1989-08-01 00:00:00

abstract：:The basis for nucleolar dominance in mouse-human cell hybrids which contained all of the mouse chromosomes but an incomplete set of human chromosomes (M greater than H) was examined at the molecular level. S1 mapping data showed that these cells had the expected levels of steady-state rRNA transcribed from mouse ribos...

journal_title：Molecular and cellular biology

authors： Miesfeld R,Sollner-Webb B,Croce C,Arnheim N

更新日期：1984-07-01 00:00:00

abstract：:We cloned a 12.3-kilobase (kb) endogenous plasmid, Ddp1, found in several wild-type and laboratory strains of Dictyostelium discoideum into pBR322. The cloned plasmids have been used to cotransform D. discoideum cells with B10S, a transformation vector carrying a gene fusion conferring resistance to G418. Whereas B10S...

journal_title：Molecular and cellular biology

authors： Firtel RA,Silan C,Ward TE,Howard P,Metz BA,Nellen W,Jacobson A

更新日期：1985-11-01 00:00:00

abstract：:The role of the target cell in its own death mediated by cytotoxic T lymphocytes (CTL) has been controversial. The ability of the pore-forming granule components of CTL to induce target cell death directly has been taken to suggest an essentially passive role for the target. This view of CTL-mediated killing ascribes ...

journal_title：Molecular and cellular biology

authors： Ucker DS,Wilson JD,Hebshi LD

更新日期：1994-01-01 00:00:00

abstract：:Human ras genes play central roles in coupling extracellular signals with complex intracellular networks controlling proliferation, differentiation, and apoptosis, among others processes. c-H-ras pre-mRNA can be alternatively processed into two mRNAs due to the inclusion or exclusion of the alternative exon IDX; this ...

journal_title：Molecular and cellular biology

authors： Guil S,Gattoni R,Carrascal M,Abián J,Stévenin J,Bach-Elias M

更新日期：2003-04-01 00:00:00

abstract：:We characterized the organization and expression of PHO5 and PHO3, the tightly linked repressible and constitutive acid phosphatase genes of Saccharomyces cerevisiae. The "constitutive" gene, PHO3, is expressed only when PHO5 is not. Altering PHO5 expression, either through promoter deletions or through mutations in t...

journal_title：Molecular and cellular biology

authors： Tait-Kamradt AG,Turner KJ,Kramer RA,Elliott QD,Bostian SJ,Thill GP,Rogers DT,Bostian KA

更新日期：1986-06-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the heterodimeric transcription factor SBF (for SCB binding factor) is composed of Swi4 and Swi6 and activates gene expression at the G(1)/S-phase transition of the mitotic cell cycle. Cell cycle commitment is associated not only with major alterations in gene expression but also with high...

journal_title：Molecular and cellular biology

authors： Baetz K,Moffat J,Haynes J,Chang M,Andrews B

更新日期：2001-10-01 00:00:00

abstract：:We have characterized features of the site recognized by a double-stranded DNA endonuclease, I-SceII, encoded by intron 4 alpha of the yeast mitochondrial COX1 gene. We determined the effects of 36 point mutations on the cleavage efficiency of natural and synthetic substrates containing the Saccharomyces capensis I-Sc...

journal_title：Molecular and cellular biology

authors： Wernette C,Saldanha R,Smith D,Ming D,Perlman PS,Butow RA

更新日期：1992-02-01 00:00:00

abstract：:Human insulin-like growth factor II (IGF-II) mRNAs are subject to site-specific endonucleolytic cleavage in the 3' untranslated region, leading to an unstable 5' cleavage product containing the IGF-II coding region and a very stable 3' cleavage product of 1.8 kb. This endonucleolytic cleavage is most probably the firs...

journal_title：Molecular and cellular biology

authors： Scheper W,Meinsma D,Holthuizen PE,Sussenbach JS

更新日期：1995-01-01 00:00:00

abstract：:We describe here a new cell line, EL2, which spontaneously arose from primary rat embryo fibroblasts and has the distinctive property of being highly susceptible to a number of different transforming genes. The high susceptibility is expressed not only in high transformation frequencies but, most importantly, in an un...

journal_title：Molecular and cellular biology

authors： Liboi E,Caruso M,Basilico C

更新日期：1984-12-01 00:00:00

abstract：:Translation initiation in eukaryotic cells is known to be a complex multistep process which involves numerous protein factors. Here we demonstrate that leaderless mRNAs with initiator Met-tRNA can bind directly to 80S mammalian ribosomes in the absence of initiation factors and that the complexes thus formed are fully...

journal_title：Molecular and cellular biology

authors： Andreev DE,Terenin IM,Dunaevsky YE,Dmitriev SE,Shatsky IN

更新日期：2006-04-01 00:00:00

abstract：:A mutation (K38R) which specifically eliminates kinase activity was created in the Drosophila melanogaster ckI gene (doubletime [dbt]). In vitro, DBT protein carrying the K38R mutation (DBT(K/R)) interacted with Period protein (PER) but lacked kinase activity. In cell culture and in flies, DBT(K/R) antagonized the pho...

journal_title：Molecular and cellular biology

authors： Muskus MJ,Preuss F,Fan JY,Bjes ES,Price JL

更新日期：2007-12-01 00:00:00

abstract：:The SNAIL transcription factor contains C-terminal tandem zinc finger motifs and an N-terminal SNAG repression domain. The members of the SNAIL family have recently emerged as major contributors to the processes of development and metastasis via the regulation of epithelial-mesenchymal transition events during embryon...

journal_title：Molecular and cellular biology

authors： Hou Z,Peng H,Ayyanathan K,Yan KP,Langer EM,Longmore GD,Rauscher FJ 3rd

更新日期：2008-05-01 00:00:00

abstract：:The immunoglobulin M heavy-chain locus contains two poly(A) sites which are alternatively expressed during B-cell differentiation. Despite its promoter proximal location, the secretory poly(A) site is not expressed in undifferentiated cells. Crucial to the activation of the secretory poly(A) site during B-cell differe...

journal_title：Molecular and cellular biology

authors： Phillips C,Pachikara N,Gunderson SI

更新日期：2004-07-01 00:00:00

abstract：:We have recently reported that the yeast chromatin-remodeling factor Swi1 can exist as a prion, [SWI(+)], demonstrating a link between prionogenesis and global transcriptional regulation. To shed light on how the Swi1 conformational switch influences Swi1 function and to define the sequence and structural requirements...

journal_title：Molecular and cellular biology

authors： Du Z,Crow ET,Kang HS,Li L

更新日期：2010-10-01 00:00:00

abstract：:We previously identified a sequence-specific erythroid cell-enriched endoribonuclease (ErEN) activity involved in the turnover of the stable alpha-globin mRNA. We now demonstrate that ErEN activity is regulated by the poly(A) tail. The unadenylated alpha-globin 3' untranslated region (3'UTR) was an efficient substrate...

journal_title：Molecular and cellular biology

authors： Wang Z,Kiledjian M

更新日期：2000-09-01 00:00:00

abstract：:Mutations in polycystin 2 (PC2), a Ca(2+)-permeable cation channel, cause autosomal dominant polycystic kidney disease. Whether PC2 functions in the endoplasmic reticulum (ER) or in the plasma membrane has been controversial. Here we generated and characterized a polyclonal antibody against PC2, determined the subcell...

journal_title：Molecular and cellular biology

authors： Luo Y,Vassilev PM,Li X,Kawanabe Y,Zhou J

更新日期：2003-04-01 00:00:00

abstract：:The gene encoding human hemojuvelin (HJV) is one of the genes that, when mutated, can cause juvenile hemochromatosis, an early-onset inherited disorder associated with iron overload. The 5' untranslated region of the human HJV mRNA has two upstream open reading frames (uORFs), with 28 and 19 codons formed by two upstr...

journal_title：Molecular and cellular biology

authors： Onofre C,Tomé F,Barbosa C,Silva AL,Romão L

更新日期：2015-04-01 00:00:00

abstract：:The tissue-specific expression of immunoglobulin genes can be partially explained by a requirement for activating factors found only in B lymphocytes and their derivatives. However, loss of immunoglobulin expression upon fusion of an immunoglobulin-producing myeloma cell with a T lymphoma cell (BW5147) or fibroblast (...

journal_title：Molecular and cellular biology

authors： Zaller DM,Yu H,Eckhardt LA

更新日期：1988-05-01 00:00:00

abstract：:The erect wing (ewg) locus of Drosophila melanogaster encodes a vital function important for the development of the nervous system and the indirect flight muscles. In order to understand the ewg function at a molecular level, cDNA clones were isolated. Sequence analysis of cDNAs revealed a single open reading frame (O...

journal_title：Molecular and cellular biology

authors： DeSimone SM,White K

更新日期：1993-06-01 00:00:00

abstract：:A mixed-oligonucleotide probe was used to identify four ras-like coding sequences in a human teratocarcinoma cDNA library. Two of these sequences resembled the rho genes, one was closely related to H-, K-, and N-ras, and one shared only the four sequence domains that define the ras gene superfamily. Homologs of the fo...

journal_title：Molecular and cellular biology

authors： Drivas GT,Shih A,Coutavas E,Rush MG,D'Eustachio P

更新日期：1990-04-01 00:00:00

abstract：:Cells of the human embryonal carcinoma line NEC14 proliferate as densely packed clusters consisting of small, polygonal stem cells and do not express a detectable level of fibronectin (FN). Upon induction of differentiation by treatment with N,N'-hexamethylene bisacetamide (HMBA), the level of FN mRNA increased steepl...

journal_title：Molecular and cellular biology

authors： Suzuki M,Oda E,Nakajima T,Sekiya S,Oda K

更新日期：1998-05-01 00:00:00

abstract：:A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction of expression of RTA was lethal. This lethality was the basis for a selection of mutations in RTA which inactivated the ...

journal_title：Molecular and cellular biology

authors： Frankel A,Schlossman D,Welsh P,Hertler A,Withers D,Johnston S

更新日期：1989-02-01 00:00:00