Carcinogenic nickel silences gene expression by chromatin condensation and DNA methylation: a new model for epigenetic carcinogens.

abstract：:We previously identified a sequence-specific erythroid cell-enriched endoribonuclease (ErEN) activity involved in the turnover of the stable alpha-globin mRNA. We now demonstrate that ErEN activity is regulated by the poly(A) tail. The unadenylated alpha-globin 3' untranslated region (3'UTR) was an efficient substrate...

journal_title：Molecular and cellular biology

authors： Wang Z,Kiledjian M

更新日期：2000-09-01 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (RB) is a potent inhibitor of cell proliferation. RB is expressed throughout the cell cycle, but its antiproliferative activity is neutralized by phosphorylation during the G(1)/S transition. RB plays an essential role in the G(1) arrest induced by a variety of growth inhibi...

journal_title：Molecular and cellular biology

authors： Knudsen KE,Booth D,Naderi S,Sever-Chroneos Z,Fribourg AF,Hunton IC,Feramisco JR,Wang JY,Knudsen ES

更新日期：2000-10-01 00:00:00

abstract：:The retinoblastoma protein (RB) represses RNA polymerase III transcription effectively both in vivo and in vitro. Here we demonstrate that the general transcription factors snRNA-activating protein complex (SNAP(c)) and TATA binding protein (TBP) are important for RB repression of human U6 snRNA gene transcription by ...

journal_title：Molecular and cellular biology

authors： Hirsch HA,Gu L,Henry RW

更新日期：2000-12-01 00:00:00

abstract：:Transcription activation of alpha-specific genes in Saccharomyces cerevisiae is regulated by two proteins, MCM1 and alpha 1, which bind to DNA sequences, called P'Q elements, found upstream of alpha-specific genes. Neither MCM1 nor alpha 1 alone binds efficiently to P'Q elements. Together, however, they bind cooperati...

journal_title：Molecular and cellular biology

authors： Hagen DC,Bruhn L,Westby CA,Sprague GF Jr

更新日期：1993-11-01 00:00:00

abstract：:The human glycoprotein IIB (GPIIB) gene is expressed only in megakaryocytes, and its promoter displays cell type specificity. We show that this specificity involved two cis-acting sequences. The first one, located at -55, contains a GATA binding site. Point mutations that abolish protein binding on this site decrease ...

journal_title：Molecular and cellular biology

authors： Lemarchandel V,Ghysdael J,Mignotte V,Rahuel C,Roméo PH

更新日期：1993-01-01 00:00:00

abstract：:The c-Jun NH2-terminal protein kinase (JNK) is a member of the mitogen-activated protein kinase (MAPK) group and is an essential component of a signaling cascade that is activated by exposure of cells to environmental stress. JNK activation is regulated by phosphorylation on both Thr and Tyr residues by a dual-specifi...

journal_title：Molecular and cellular biology

authors： Tournier C,Whitmarsh AJ,Cavanagh J,Barrett T,Davis RJ

更新日期：1999-02-01 00:00:00

abstract：:Little is known about the posttranslational control of the cyclin-dependent protein kinase (CDK) inhibitor p21. We describe here a transient phosphorylation of p21 in the G2/M phase. G2/M-phosphorylated p21 is short-lived relative to hypophosphorylated p21. p21 becomes nuclear during S phase, prior to its phosphorylat...

journal_title：Molecular and cellular biology

authors： Dash BC,El-Deiry WS

更新日期：2005-04-01 00:00:00

abstract：:Transcriptional insulators are cis regulatory elements that organize chromatin into independently regulated domains. At the imprinted murine Igf2/H19 locus, the H19-ICR insulator prevents the activation of the Igf2 promoter on the maternal allele by enhancers that activate H19 on the same chromosome. Given the well-de...

journal_title：Molecular and cellular biology

authors： Singh V,Srivastava M

更新日期：2008-06-01 00:00:00

abstract：:In Aspergillus nidulans, a fungus that possesses negligible, if any, levels of methylation in its genome, low concentrations of 5-azacytidine (5-AC) convert a high percentage of the cell population to fluffy phenotypic variants through a heritable modification of a single nuclear gene (M. Tamame, F. Antequera, J. R. V...

journal_title：Molecular and cellular biology

authors： Tamame M,Antequera F,Santos E

更新日期：1988-08-01 00:00:00

abstract：:Notch signaling requires a series of proteolytic cleavage events to release the Notch intracellular domain (NICD) that functions directly in signal transduction. The Notch receptor is locked down in a protease-resistant state by a negative regulatory region (NRR) that protects an ADAM (a disintegrin and metalloproteas...

journal_title：Molecular and cellular biology

authors： Bozkulak EC,Weinmaster G

更新日期：2009-11-01 00:00:00

abstract：:Our previous studies of Saccharomyces cerevisiae described a gene repression mechanism where the transcription of intergenic noncoding DNA (ncDNA) (SRG1) assembles nucleosomes across the promoter of the adjacent SER3 gene that interfere with the binding of transcription factors. To investigate the role of histones in ...

journal_title：Molecular and cellular biology

authors： Hainer SJ,Martens JA

更新日期：2011-09-01 00:00:00

abstract：:STAT3 is a ubiquitous transcription factor that is indispensable during early embryogenesis. To study the functions of STAT3 postnatally, we generated conditional STAT3-deficient mice. To that end, STAT3(lox/lox) mice were crossed with mice expressing Cre under the control of rat insulin II gene promoter (RIP-Cre mice...

journal_title：Molecular and cellular biology

authors： Cui Y,Huang L,Elefteriou F,Yang G,Shelton JM,Giles JE,Oz OK,Pourbahrami T,Lu CY,Richardson JA,Karsenty G,Li C

更新日期：2004-01-01 00:00:00

abstract：:As shown by Southern blot analysis, the metallothionein-1 (MT-1) genes in rats comprise a multigene family. We present the sequence of the MT-1 structural gene and compare its features with other metallothionein genes. Three MT-1 pseudogenes which we sequenced apparently arose by reverse transcription of processed mRN...

journal_title：Molecular and cellular biology

authors： Andersen RD,Birren BW,Taplitz SJ,Herschman HR

更新日期：1986-01-01 00:00:00

abstract：:Coordination of skeletal muscle growth and metabolism with nutrient availability is critical for metabolic homeostasis. To establish the role of insulin-like signaling in this process, we used muscle creatine kinase (MCK)-Cre to disrupt expression of insulin receptor substrates Irs1 and Irs2 in mouse skeletal/cardiac ...

journal_title：Molecular and cellular biology

authors： Long YC,Cheng Z,Copps KD,White MF

更新日期：2011-02-01 00:00:00

abstract：:Insulin stimulates glucose transport into fat and muscle cells by increasing the exocytic trafficking rate of the GLUT4 facilitative glucose transporter from intracellular stores to the plasma membrane. Delivery of GLUT4 to the plasma membrane is mediated by formation of functional SNARE complexes containing syntaxin4...

journal_title：Molecular and cellular biology

authors： Kioumourtzoglou D,Gould GW,Bryant NJ

更新日期：2014-04-01 00:00:00

abstract：:Pancreatic beta-cell-type-specific transcription of the insulin gene is principally regulated by a single cis-acting DNA sequence element, termed the insulin control element (ICE), which is found within the 5'-flanking region of the gene. The ICE activator is a heteromeric complex composed of an islet alpha/beta-cell-...

journal_title：Molecular and cellular biology

authors： Robinson GL,Cordle SR,Henderson E,Weil PA,Teitelman G,Stein R

更新日期：1994-10-01 00:00:00

abstract：:We have characterized a pathway for nuclear export of the glucocorticoid receptor (GR) in mammalian cells. This pathway involves the Ca2+ -binding protein calreticulin (CRT), which directly contacts the DNA binding domain (DBD) of GR and facilitates its delivery from the nucleus to the cytoplasm. In the present study,...

journal_title：Molecular and cellular biology

authors： Holaska JM,Black BE,Rastinejad F,Paschal BM

更新日期：2002-09-01 00:00:00

abstract：:The transcription of meiosis-specific genes, as well as the initiation of meiosis, in the budding yeast Saccharomyces cerevisiae depends on IME1. IME1 encodes a transcriptional activator which lacks known DNA binding motifs. In this study we have determined the mode by which Ime1 specifically activates the transcripti...

journal_title：Molecular and cellular biology

authors： Rubin-Bejerano I,Mandel S,Robzyk K,Kassir Y

更新日期：1996-05-01 00:00:00

abstract：:When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority of HCF-1 in the cell is a complex of associated amino (HCF-1(N))- and carboxy (HCF-1(C))-terminal subunits that result fr...

journal_title：Molecular and cellular biology

authors： Wilson AC,Boutros M,Johnson KM,Herr W

更新日期：2000-09-01 00:00:00

abstract：:Two mutator genes of mammalian cells were demonstrated. One was associated with the ribonucleoside diphosphate reductase, and the other was associated with an extreme adenosine sensitivity. ...

journal_title：Molecular and cellular biology

authors： Chan VL,Guttman S,Juranka P

更新日期：1981-06-01 00:00:00

abstract：:ADR1 is a yeast transcription factor that contains two zinc fingers of the Cys-2-His-2 (C2H2) class. Mutations that change the specificity of DNA binding of ADR1 to its target site, upstream activation sequence 1 (UAS1), have been identified at three positions in the first zinc finger. Mutations Arg-115 to Gln, His-11...

journal_title：Molecular and cellular biology

authors： Thukral SK,Morrison ML,Young ET

更新日期：1992-06-01 00:00:00

abstract：:Y-27632, an inhibitor of the Rho-associated kinase ROCK, is a therapeutic lead for Huntington disease (HD). The downstream targets that mediate its inhibitory effects on huntingtin (Htt) aggregation and toxicity are unknown. We have identified profilin, a small actin-binding factor that also interacts with Htt, as bei...

journal_title：Molecular and cellular biology

authors： Shao J,Welch WJ,Diprospero NA,Diamond MI

更新日期：2008-09-01 00:00:00

abstract：:The expression and distribution of DNA polymerase alpha was measured by cytometry and confocal laser scanning microscopy. Expression was proportional to DNA content in proliferating cells, while only S-phase cells retained DNA polymerase alpha after detergent extraction. Nuclear DNA polymerase alpha binding may be one...

journal_title：Molecular and cellular biology

authors： Stokke T,Erikstein B,Holte H,Funderud S,Steen HB

更新日期：1991-06-01 00:00:00

abstract：:In Chlamydomonas reinhardi the chloroplast DNA (ch;DNA) of mating type plus cells undergoes cyclical methylation and demethylation during the life cycle. Methylation occurs during gametogenesis, and fully differentiated gametes can be dedifferentiated back to vegetative cells which contain nonmethylated chlDNA by the ...

journal_title：Molecular and cellular biology

authors： Sano H,Grabowy C,Sager R

更新日期：1984-10-01 00:00:00

abstract：:Fragments of African green monkey (Cercopithecus aethiops) DNA (3.5 to 18.0 kilobases) were inserted downstream from the thymidine kinase (TK, tk) coding region in pTK206/SV010, a gene construct which lacks both copies of the hexanucleotide 5'-AATAAA-3' and contains a simian virus 40 origin of replication, allowing it...

journal_title：Molecular and cellular biology

authors： Santangelo GM,Cole CN

更新日期：1983-04-01 00:00:00

abstract：:B-lymphocyte development requires the basic helix-loop-helix proteins encoded by the E2A gene. In this study, the control mechanism of E2A was further explored by disruption of the E2A-related genes, E2-2 and HEB. In contrast to E2A, E2-2 and HEB are not essential for the establishment of the B-cell lineage. However, ...

journal_title：Molecular and cellular biology

authors： Zhuang Y,Cheng P,Weintraub H

更新日期：1996-06-01 00:00:00

abstract：:Scaffold proteins have been established as important mediators of signal transduction specificity. The insulin receptor substrate (IRS) proteins represent a critical group of scaffold proteins that are required for signal transduction by the insulin receptor, including the activation of phosphatidylinositol 3 kinase. ...

journal_title：Molecular and cellular biology

authors： Standen CL,Kennedy NJ,Flavell RA,Davis RJ

更新日期：2009-09-01 00:00:00

abstract：:A previously uncharacterized Saccharomyces cerevisiae gene, FAL1, was found by sequence comparison as a homolog of the eukaryotic translation initiation factor 4A (eIF4A). Fal1p has 55% identity and 73% similarity on the amino acid level to yeast eIF4A, the prototype of ATP-dependent RNA helicases of the DEAD-box prot...

journal_title：Molecular and cellular biology

authors： Kressler D,de la Cruz J,Rojo M,Linder P

更新日期：1997-12-01 00:00:00

abstract：:To understand the mechanisms by which the chromatin-remodeling SWI/SNF complex interacts with DNA and alters nucleosome organization, we have imaged the SWI/SNF complex with both naked DNA and nucleosomal arrays by using energy-filtered microscopy. By making ATP-independent contacts with DNA at multiple sites on its s...

journal_title：Molecular and cellular biology

authors： Bazett-Jones DP,Côté J,Landel CC,Peterson CL,Workman JL

更新日期：1999-02-01 00:00:00

abstract：:Poliovirus polysomal RNA is naturally uncapped, and as such, its translation must bypass any 5' cap-dependent ribosome recognition event. To elucidate the manner by which poliovirus mRNA is translated, we have determined the translational efficiencies of a series of deletion mutants within the 5' noncoding region of t...

journal_title：Molecular and cellular biology

authors： Pelletier J,Kaplan G,Racaniello VR,Sonenberg N

更新日期：1988-03-01 00:00:00