New rat cell line that is highly susceptible to transformation by several oncogenes.

abstract：:To distinguish among possible mechanisms of repair of a double-strand break (DSB) by gene conversion in budding yeast, Saccharomyces cerevisiae, we employed isotope density transfer to analyze budding yeast mating type (MAT) gene switching in G2/M-arrested cells. Both of the newly synthesized DNA strands created durin...

journal_title：Molecular and cellular biology

authors： Ira G,Satory D,Haber JE

更新日期：2006-12-01 00:00:00

abstract：:The GCN4 gene encodes a positive effector of amino acid biosynthetic genes in Saccharomyces cerevisiae. Genetic analysis has suggested that GCN4 is regulated by a hierarchy of interacting positive and negative effectors in response to amino acid starvation. Results presented here for a GCN4-lacZ gene fusion support th...

journal_title：Molecular and cellular biology

authors： Hinnebusch AG

更新日期：1985-09-01 00:00:00

abstract：:Signaling via the pre-T-cell receptor (pre-TCR), along with associated signals from Notch and chemokine receptors, regulates the β-selection checkpoint that operates on CD4(-) CD8(-) doubly negative (DN) thymocytes. Since many hematopoietic malignancies arise at the immature developmental stages of lymphocytes, unders...

journal_title：Molecular and cellular biology

authors： Trampont PC,Zhang L,Giles AJ,Walk SF,Gu JJ,Pendergast AM,Ravichandran KS

更新日期：2015-04-01 00:00:00

abstract：:Mouse mammary tumor virus (MMTV) has long been implicated in mouse mammary carcinogenesis, and it is now well established that the long terminal repeat (LTR) contains regulatory sequences responsible for glucocorticoid-mediated induction of viral RNA. However, we have demonstrated previously that androgens as well as ...

journal_title：Molecular and cellular biology

authors： Darbre P,Page M,King RJ

更新日期：1986-08-01 00:00:00

abstract：:Retinoid X receptors (RXR) have been identified as common subunits in the regulation of multiple hormonal signaling pathways. Using circular permutation and phasing analysis of specific response elements, we present evidence that RXR-retinoic acid receptor and RXR-thyroid hormone receptor heterodimer or RXR-RXR homodi...

journal_title：Molecular and cellular biology

authors： Lu XP,Eberhardt NL,Pfahl M

更新日期：1993-10-01 00:00:00

abstract：:Gap junctions are membrane channels that permit the interchange of ions and other low-molecular-weight molecules between adjacent cells. Rous sarcoma virus (RSV)-induced transformation is marked by an early and profound disruption of gap-junctional communication, suggesting that these membrane structures may serve as ...

journal_title：Molecular and cellular biology

authors： Crow DS,Beyer EC,Paul DL,Kobe SS,Lau AF

更新日期：1990-04-01 00:00:00

abstract：:The mouse urokinase-type plasminogen activator (uPA) gene was used as a model macrophage colony-stimulating factor 1 (CSF-1)-inducible gene to investigate CSF-1 signalling pathways. Nuclear run-on analysis showed that induction of uPA mRNA by CSF-1 and phorbol myristate acetate (PMA) was at the transcriptional level i...

journal_title：Molecular and cellular biology

authors： Stacey KJ,Fowles LF,Colman MS,Ostrowski MC,Hume DA

更新日期：1995-06-01 00:00:00

abstract：:We analyzed sites of macronuclear telomere addition at a single genetic locus in Paramecium tetraurelia. We showed that in homozygous wild-type cells, differential genomic processing during macronuclear development resulted in the A surface antigen gene being located 8, 13, or 26 kilobases upstream from a macronuclear...

journal_title：Molecular and cellular biology

authors： Forney JD,Blackburn EH

更新日期：1988-01-01 00:00:00

abstract：:Methylation of histone H3 on lysine 9 or 27 is crucial for heterochromatin formation. Previously considered hallmarks of, respectively, constitutive and facultative heterochromatin, recent evidence has accumulated in favor of coexistence of these two marks and their cooperation in gene silencing maintenance. H3K9me2/3...

journal_title：Molecular and cellular biology

authors： Boros J,Arnoult N,Stroobant V,Collet JF,Decottignies A

更新日期：2014-10-01 00:00:00

abstract：:HO endonuclease-induced double-strand breaks in Saccharomyces cerevisiae can undergo recombination by two distinct and competing pathways. In a plasmid containing a direct repeat, in which one repeat is interrupted by an HO endonuclease cut site, gap repair yields gene conversions while single-strand annealing produce...

journal_title：Molecular and cellular biology

authors： Fishman-Lobell J,Rudin N,Haber JE

更新日期：1992-03-01 00:00:00

abstract：:To examine the role of clathrin-dependent insulin receptor internalization in insulin-stimulated signal transduction events, we expressed a dominant-interfering mutant of dynamin (K44A/dynamin) by using a recombinant adenovirus in the H4IIE hepatoma and 3T3L1 adipocyte cell lines. Expression of K44A/dynamin inhibited ...

journal_title：Molecular and cellular biology

authors： Ceresa BP,Kao AW,Santeler SR,Pessin JE

更新日期：1998-07-01 00:00:00

abstract：:In CBFbeta-SMMHC, core binding factor beta (CBFbeta) is fused to the alpha-helical rod domain of smooth muscle myosin heavy chain (SMMHC). We generated Ba/F3 hematopoietic cells expressing a CBFbeta-SMMHC variant lacking 28 amino acids homologous to the assembly competence domain (ACD) required for multimerization of ...

journal_title：Molecular and cellular biology

authors： Kummalue T,Lou J,Friedman AD

更新日期：2002-12-01 00:00:00

abstract：:Vimentin is a tissue-specific, developmentally regulated member of the intermediate filament protein family normally expressed in cells of mesenchymal origin. Transcription factors which recognize specific cis-acting elements of the chicken gene include Sp-1 and the 95-kDa silencer protein which binds to a 40-bp silen...

journal_title：Molecular and cellular biology

authors： Stover DM,Zehner ZE

更新日期：1992-05-01 00:00:00

abstract：:The 5'-flanking DNA sequences involved in the specific and efficient transcription of the polyoma virus early region have been investigated. Sequence requirements for efficient in vivo expression differed from those in vitro. Deletion of DNA located between 200 and 400 base pairs before the principal cap sites severel...

journal_title：Molecular and cellular biology

authors： Jat P,Novak U,Cowie A,Tyndall C,Kamen R

更新日期：1982-07-01 00:00:00

abstract：:Replication protein A (RPA) is required for simian virus 40-directed DNA replication in vitro and for nucleotide excision repair (NER). Here we report that RPA and the human repair protein XPA specifically interact both in vitro and in vivo. Mapping of the RPA-interactive domains in XPA revealed that both of the large...

journal_title：Molecular and cellular biology

authors： Li L,Lu X,Peterson CA,Legerski RJ

更新日期：1995-10-01 00:00:00

abstract：:Eight strains of Chinese hamster ovary (CHO) cells having an assembly-defective beta-tubulin were found among revertants of strain Cmd 4, a mutant with a conditional lethal mutation in a beta-tubulin gene (F. Cabral, M. E. Sobel, and M. M. Gottesman, Cell 20:29-36, 1980). The altered beta-tubulins in these strains hav...

journal_title：Molecular and cellular biology

authors： Boggs B,Cabral F

更新日期：1987-08-01 00:00:00

abstract：:We analyzed the impact of a GAGA element on a transgenic promoter in Drosophila melanogaster that was activated by proteins composed of the Tet(on) DNA binding domain and either the heat shock factor (HSF) activation domain or a potent subdomain of VP16. Permanganate footprinting was used to monitor polymerase II (Pol...

journal_title：Molecular and cellular biology

authors： Wang YV,Tang H,Gilmour DS

更新日期：2005-05-01 00:00:00

abstract：:The neutral/neutral two-dimensional (2-D) gel replicon mapping technique has been used to great advantage to localize and characterize origins of replication. Interestingly, many yeast origins display a composite pattern consisting of both a bubble arc and a single-fork arc. Moreover, in every instance in which neutra...

journal_title：Molecular and cellular biology

authors： Kalejta RF,Hamlin JL

更新日期：1996-09-01 00:00:00

abstract：:Peroxisomal PTS2-dependent matrix protein import starts with the recognition of the PTS2 targeting signal by the import receptor Pex7p. Subsequently, the formed Pex7p/cargo complex is transported from the cytosol to the peroxisomal docking complex, consisting of Pex13p and Pex14p. In Saccharomyces cerevisiae, the latt...

journal_title：Molecular and cellular biology

authors： Stein K,Schell-Steven A,Erdmann R,Rottensteiner H

更新日期：2002-09-01 00:00:00

abstract：:The yeast gene MCK1 encodes a serine/threonine protein kinase that is thought to function in regulating kinetochore activity and entry into meiosis. Disruption of MCK1 confers a cold-sensitive phenotype, a temperature-sensitive phenotype, and sensitivity to the microtubule-destabilizing drug benomyl and leads to loss ...

journal_title：Molecular and cellular biology

authors： Puziss JW,Hardy TA,Johnson RB,Roach PJ,Hieter P

更新日期：1994-01-01 00:00:00

abstract：:Galanin is a neuropeptide implicated in the regulation of feeding, reproduction, cognition, nociception, and seizure susceptibility. There are three known galanin receptor (GALR) subtypes (GALR1, GALR2, and GALR3), which bind to galanin with different affinities and have their own unique distributions, signaling mecha...

journal_title：Molecular and cellular biology

authors： Gottsch ML,Zeng H,Hohmann JG,Weinshenker D,Clifton DK,Steiner RA

更新日期：2005-06-01 00:00:00

abstract：:Gene targeting is a very powerful tool for studying mammalian development and physiology and for creating models of human diseases. In many instances, however, it is desirable to study different modifications of a target gene, but this is limited by the generally low frequency of homologous recombination in mammalian ...

journal_title：Molecular and cellular biology

authors： Cohen-Tannoudji M,Robine S,Choulika A,Pinto D,El Marjou F,Babinet C,Louvard D,Jaisser F

更新日期：1998-03-01 00:00:00

abstract：:Filopodia are dynamic structures found at the leading edges of most migrating cells. IRSp53 plays a role in filopodium dynamics by coupling actin elongation with membrane protrusion. IRSp53 is a Cdc42 effector protein that contains an N-terminal inverse-BAR (Bin-amphipysin-Rvs) domain (IRSp53/MIM homology domain [IMD]...

journal_title：Molecular and cellular biology

authors： Robens JM,Yeow-Fong L,Ng E,Hall C,Manser E

更新日期：2010-02-01 00:00:00

abstract：:To determine the role of poly(A) polymerase in 3'-end processing of mRNA, the effect of purified poly(A) polymerase antibodies on endonucleolytic cleavage and polyadenylation was studied in HeLa nuclear extracts, using adenovirus L3 pre-mRNA as the substrate. Both Mg2+- and Mn2+-dependent reactions catalyzing addition...

journal_title：Molecular and cellular biology

authors： Terns MP,Jacob ST

更新日期：1989-04-01 00:00:00

abstract：:The dorsal protein (DL) regulates the transcriptional activity of several genes that determine cell fate along the dorsoventral axis of the Drosophila melanogaster embryo. DL is present at high levels in ventral nuclei, where it activates some genes (twi and sna) and represses others (zen, dpp, and tld). DL shows homo...

journal_title：Molecular and cellular biology

authors： Kirov N,Childs S,O'Connor M,Rushlow C

更新日期：1994-01-01 00:00:00

abstract：:Signal transduction by antigen receptors and some Fc receptors requires the activation of a family of receptor-associated transmembrane accessory proteins. One common feature of the cytoplasmic domains of these accessory molecules is the presence is at least two YXXA repeats that are potential sites for interaction wi...

journal_title：Molecular and cellular biology

authors： Burkhardt AL,Costa T,Misulovin Z,Stealy B,Bolen JB,Nussenzweig MC

更新日期：1994-02-01 00:00:00

abstract：:Members of the transforming growth factor beta (TGF-beta) family transduce signals through Smad proteins. Smad signaling can be regulated by the Ras/Erk/mitogen-activated protein pathway in response to receptor tyrosine kinase activation and the gamma interferon pathway and also by the functional interaction of Smad2 ...

journal_title：Molecular and cellular biology

authors： Wicks SJ,Lui S,Abdel-Wahab N,Mason RM,Chantry A

更新日期：2000-11-01 00:00:00

abstract：:Interleukin-5 (IL-5) and IL-6 have both been reported to act as B-cell differentiation factors by stimulating activated B cells to secrete antibody. However, it has not been possible to directly compare the effects of these two lymphokines because of the lack of a suitable B-cell line capable of responding to both. We...

journal_title：Molecular and cellular biology

authors： Randall TD,Lund FE,Brewer JW,Aldridge C,Wall R,Corley RB

更新日期：1993-07-01 00:00:00

abstract：:Complete replication of the genome is an essential prerequisite for normal cell division, but a variety of factors can block the replisome, triggering replication stress and potentially causing mutation or cell death. The cellular response to replication stress involves recruitment of proteins to stabilize the replica...

journal_title：Molecular and cellular biology

authors： Her J,Ray C,Altshuler J,Zheng H,Bunting SF

更新日期：2018-03-29 00:00:00

abstract：:HIRA-like (Hir) proteins are evolutionarily conserved and are implicated in the assembly of repressive chromatin. In Saccharomyces cerevisiae, Hir proteins contribute to the function of centromeres. However, S. cerevisiae has point centromeres that are structurally different from the complex centromeres of metazoans. ...

journal_title：Molecular and cellular biology

authors： Blackwell C,Martin KA,Greenall A,Pidoux A,Allshire RC,Whitehall SK

更新日期：2004-05-01 00:00:00