Abstract:
:To distinguish among possible mechanisms of repair of a double-strand break (DSB) by gene conversion in budding yeast, Saccharomyces cerevisiae, we employed isotope density transfer to analyze budding yeast mating type (MAT) gene switching in G2/M-arrested cells. Both of the newly synthesized DNA strands created during gene conversion are found at the repaired locus, leaving the donor unchanged. These results support suggestions that mitotic DSBs are primarily repaired by a synthesis-dependent strand-annealing mechanism. We also show that the proportion of crossing-over associated with DSB-induced ectopic recombination is not affected by the presence of nonhomologous sequences at one or both ends of the DSB or the presence of additional sequences that must be copied from the donor.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Ira G,Satory D,Haber JEdoi
10.1128/MCB.01654-06subject
Has Abstractpub_date
2006-12-01 00:00:00pages
9424-9issue
24eissn
0270-7306issn
1098-5549pii
MCB.01654-06journal_volume
26pub_type
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