Nonsense-containing mRNAs that accumulate in the absence of a functional nonsense-mediated mRNA decay pathway are destabilized rapidly upon its restitution.


:Nonsense-mediated mRNA decay (NMD) is a conserved proofreading mechanism that protects eukaryotic cells from the potentially deleterious effects of truncated proteins. Studies of Saccharomyces cerevisiae imply that NMD is a predominantly cytoplasmic decay pathway, while studies of mammalian systems suggest that decay of most substrate mRNAs may occur while they are still associated with the nucleus, possibly during a round of translation that occurs during their export to the cytoplasm. Complete entry of the latter mRNAs into the cytoplasm appears to render them immune to further NMD; i.e., they escape further susceptibility to this decay pathway. To determine if yeast cytoplasmic nonsense-containing mRNAs that evade decay are subsequently immune to NMD, we examined the consequences of placing each of the three UPF/NMD genes under the control of a galactose-inducible promoter. The decay kinetics of ADE2 and PGK1 nonsense-containing mRNAs were then analyzed when expression of UPF1, NMD2, or UPF3 was either repressed or subsequently induced. Results from these experiments demonstrated that activation of NMD caused rapid and immediate degradation of both substrate transcripts, with half-lives of both stable mRNA populations shortened to approximately 7 min. These findings make it unlikely that yeast nonsense-containing mRNAs can escape degradation by NMD and indicate that such mRNAs are available to this decay pathway at each round of translation.


Mol Cell Biol


Maderazo AB,Belk JP,He F,Jacobson A




Has Abstract


2003-02-01 00:00:00












  • The NF45/NF90 Heterodimer Contributes to the Biogenesis of 60S Ribosomal Subunits and Influences Nucleolar Morphology.

    abstract::The interleukin enhancer binding factors ILF2 (NF45) and ILF3 (NF90/NF110) have been implicated in various cellular pathways, such as transcription, microRNA (miRNA) processing, DNA repair, and translation, in mammalian cells. Using tandem affinity purification, we identified human NF45 and NF90 as components of precu...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Wandrey F,Montellese C,Koos K,Badertscher L,Bammert L,Cook AG,Zemp I,Horvath P,Kutay U

    更新日期:2015-10-01 00:00:00

  • Molecular analysis of G+C-rich upstream sequences regulating transcription of the human carbonic anhydrase II gene.

    abstract::The upstream promoter sequences of the human carbonic anhydrase II (CA II) gene have been studied by 5' deletion analysis. Promoter activity was assayed by transfection and chloramphenicol acetyltransferase assay in both human HeLa cells and murine L cells. This investigation showed that the CA II promoter is comparab...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Shapiro LH,Venta PJ,Tashian RE

    更新日期:1987-12-01 00:00:00

  • Quail Pax-6 (Pax-QNR) mRNAs are expressed from two promoters used differentially during retina development and neuronal differentiation.

    abstract::During investigations on the regulation of the Pax-6 gene, we characterized a cDNA from quail neuroretina showing a 5' untranslated region distinct from that previously described and initiated from an internal promoter. Using RNase protection and primer extension mapping, we localized this second quail Pax-6 promoter,...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Plaza S,Dozier C,Turque N,Saule S

    更新日期:1995-06-01 00:00:00

  • Transcriptional activity of neural retina leucine zipper (Nrl) is regulated by c-Jun N-terminal kinase and Tip60 during retina development.

    abstract::Neural retina leucine zipper (Nrl), a key basic motif leucine zipper (bZIP) transcription factor, modulates rod photoreceptor differentiation by activating rod-specific target genes. In searching for factors that might couple with Nrl to modulate its transcriptional activity through posttranslational modification, we ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kim JW,Jang SM,Kim CH,An JH,Choi KH

    更新日期:2012-05-01 00:00:00

  • Glycosylation and secretion of human tissue plasminogen activator in recombinant baculovirus-infected insect cells.

    abstract::Cell lines established from the lepidopteran insect Spodoptera frugiperda (fall armyworm; Sf9) are used routinely as hosts for the expression of foreign proteins by recombinant baculovirus vectors. We have examined the pathway of protein glycosylation and secretion in these cells, using human tissue plasminogen activa...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Jarvis DL,Summers MD

    更新日期:1989-01-01 00:00:00

  • Genetic mapping of Ty elements in Saccharomyces cerevisiae.

    abstract::We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a recipro...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Klein HL,Petes TD

    更新日期:1984-02-01 00:00:00

  • Differential regulation of human interferon A gene expression by interferon regulatory factors 3 and 7.

    abstract::Differential expression of the human interferon A (IFN-A) gene cluster is modulated following paramyxovirus infection by the relative amounts of active interferon regulatory factor 3 (IRF-3) and IRF-7. IRF-3 expression activates predominantly IFN-A1 and IFN-B, while IRF-7 expression induces multiple IFN-A genes. IFN-A...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Génin P,Lin R,Hiscott J,Civas A

    更新日期:2009-06-01 00:00:00

  • Interaction of the viral activator protein ICP4 with TFIID through TAF250.

    abstract::ICP4 of herpes simplex virus is responsible for the activation of viral transcription during infection. It also efficiently activates and represses transcription in vitro depending on the promoter context. The contacts made between ICP4 and the cellular proteins that result in activated transcription have not been ide...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Carrozza MJ,DeLuca NA

    更新日期:1996-06-01 00:00:00

  • Repression of CDK1 and other genes with CDE and CHR promoter elements during DNA damage-induced G(2)/M arrest in human cells.

    abstract::Entry into mitosis is controlled by the cyclin-dependent kinase CDK1 and can be delayed in response to DNA damage. In some systems, such G(2)/M arrest has been shown to reflect the stabilization of inhibitory phosphorylation sites on CDK1. In human cells, full G(2) arrest appears to involve additional mechanisms. We d...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Badie C,Itzhaki JE,Sullivan MJ,Carpenter AJ,Porter AC

    更新日期:2000-04-01 00:00:00

  • Transcription alters chromosomal locations of cohesin in Saccharomyces cerevisiae.

    abstract::In eukaryotic cells, cohesion between sister chromatids allows chromosomes to biorient on the metaphase plate and holds them together until they separate into daughter cells during mitosis. Cohesion is mediated by the cohesin protein complex. Although the association of this complex with particular regions of the geno...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bausch C,Noone S,Henry JM,Gaudenz K,Sanderson B,Seidel C,Gerton JL

    更新日期:2007-12-01 00:00:00

  • Transcription factor GATA-4 regulates cardiac muscle-specific expression of the alpha-myosin heavy-chain gene.

    abstract::The alpha-myosin heavy-chain (alpha-MHC) gene is the major structural protein in the adult rodent myocardium. Its expression is restricted to the heart by a complex interplay of trans-acting factors and their cis-acting sites. However, to date, the factors that have been shown to regulate expression of this gene have ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Molkentin JD,Kalvakolanu DV,Markham BE

    更新日期:1994-07-01 00:00:00

  • Function and control of RNA polymerase II C-terminal domain phosphorylation in vertebrate transcription and RNA processing.

    abstract::The C-terminal domain of the RNA polymerase II largest subunit (the Rpb1 CTD) is composed of tandem heptad repeats of the consensus sequence Y(1)S(2)P(3)T(4)S(5)P(6)S(7). We reported previously that Thr 4 is phosphorylated and functions in histone mRNA 3'-end formation in chicken DT40 cells. Here, we have extended our...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hsin JP,Xiang K,Manley JL

    更新日期:2014-07-01 00:00:00

  • Dynamic localization and function of Bni1p at the sites of directed growth in Saccharomyces cerevisiae.

    abstract::Formin homology (FH) proteins are implicated in cell polarization and cytokinesis through actin organization. There are two FH proteins in the yeast Saccharomyces cerevisiae, Bni1p and Bnr1p. Bni1p physically interacts with Rho family small G proteins (Rho1p and Cdc42p), actin, two actin-binding proteins (profilin and...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ozaki-Kuroda K,Yamamoto Y,Nohara H,Kinoshita M,Fujiwara T,Irie K,Takai Y

    更新日期:2001-02-01 00:00:00

  • Restriction endonuclease activity induced by PBCV-1 virus infection of a Chlorella-like green alga.

    abstract::An enzyme was isolated from a eucaryotic, Chlorella-like green alga infected with the virus PBCV-1 which exhibits type II restriction endonuclease activity. The enzyme recognized the sequence GATC and cleaved DNA 5' to the G. Methylation of deoxyadenosine in the GATC sequence inhibited enzyme activity. In vitro the en...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Xia YN,Burbank DE,Uher L,Rabussay D,Van Etten JL

    更新日期:1986-05-01 00:00:00

  • Tyrosyl and cyclic AMP-dependent protein kinase activities in BHK cells that express viral pp60src.

    abstract::Protein kinase activities were measured in Rous sarcoma virus-infected baby hamster kidney (BHK) cells that express v-src (BHK [v-src]) and compared with those of revertant and control BHK cells. We observed about a fivefold-higher tyrosine phosphorylating activity in BHK (v-src) cell extracts, which was due to src bu...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Clinton GM,Roskoski R Jr

    更新日期:1984-05-01 00:00:00

  • Activation of the DNA damage checkpoint in yeast lacking the histone chaperone anti-silencing function 1.

    abstract::The packaging of the eukaryotic genome into chromatin is likely to be important for the maintenance of genomic integrity. Chromatin structures are assembled onto newly synthesized DNA by the action of chromatin assembly factors, including anti-silencing function 1 (ASF1). To investigate the role of chromatin structure...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ramey CJ,Howar S,Adkins M,Linger J,Spicer J,Tyler JK

    更新日期:2004-12-01 00:00:00

  • Major Egr3 isoforms are generated via alternate translation start sites and differ in their abilities to activate transcription.

    abstract::In previous studies, we detected a major, unidentified Egr response element (ERE) binding complex in brain extracts. We now report that this complex contains a truncated isoform of Egr3 generated by use of an alternate translation start site at methionine 106. Furthermore, the ERE binding complex previously thought to...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: O'Donovan KJ,Baraban JM

    更新日期:1999-07-01 00:00:00

  • Delimiting regulatory sequences of the Drosophila melanogaster Ddc gene.

    abstract::We delimited sequences necessary for in vivo expression of the Drosophila melanogaster dopa decarboxylase gene Ddc. The expression of in vitro-altered genes was assayed following germ line integration via P-element vectors. Sequences between -209 and -24 were necessary for normally regulated expression, although genes...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hirsh J,Morgan BA,Scholnick SB

    更新日期:1986-12-01 00:00:00

  • An intronic locus control region plays an essential role in the establishment of an autonomous hepatic chromatin domain for the human vitamin D-binding protein gene.

    abstract::The human vitamin D-binding protein (hDBP) gene exists in a cluster of four liver-expressed genes. A minimal hDBP transgene, containing a defined set of liver-specific DNase I hypersensitive sites (HSs), is robustly expressed in mouse liver in a copy-number-dependent manner. Here we evaluate these HSs for function. De...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hiroki T,Liebhaber SA,Cooke NE

    更新日期:2007-11-01 00:00:00

  • B-lymphocyte development is regulated by the combined dosage of three basic helix-loop-helix genes, E2A, E2-2, and HEB.

    abstract::B-lymphocyte development requires the basic helix-loop-helix proteins encoded by the E2A gene. In this study, the control mechanism of E2A was further explored by disruption of the E2A-related genes, E2-2 and HEB. In contrast to E2A, E2-2 and HEB are not essential for the establishment of the B-cell lineage. However, ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zhuang Y,Cheng P,Weintraub H

    更新日期:1996-06-01 00:00:00

  • Stably integrated mouse mammary tumor virus long terminal repeat DNA requires the octamer motifs for basal promoter activity.

    abstract::In the mouse mammary tumor virus promoter, a tandem of octamer motifs, recognized by ubiquitous and tissue-restricted Oct transcription factors, is located upstream of the TATA box and next to a binding site for the transcription factor nuclear factor I (NF-I). Their function was investigated with mutant long terminal...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Buetti E

    更新日期:1994-02-01 00:00:00

  • A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus.

    abstract::The human immunodeficiency virus type 1 Rev protein contains a nuclear export signal (NES) that is required for Rev-mediated RNA export in mammals as well as in the yeast Saccharomyces cerevisiae. The Rev NES has been shown to specifically interact with a human (hRIP/RAB1) and a yeast (yRip1p) protein in the two-hybri...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Stutz F,Izaurralde E,Mattaj IW,Rosbash M

    更新日期:1996-12-01 00:00:00

  • Critical role for a single leucine residue in leukemia induction by E2A-PBX1.

    abstract::In roughly 5% of cases of acute lymphoblastic leukemia, a chromosomal translocation leads to expression of the oncogenic protein E2A-PBX1. The N-terminal portion of E2A-PBX1, encoded by the E2A gene, is identical in sequence to the corresponding portion of the E proteins E12/E47 and includes transcriptional activation...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bayly R,Murase T,Hyndman BD,Savage R,Nurmohamed S,Munro K,Casselman R,Smith SP,LeBrun DP

    更新日期:2006-09-01 00:00:00

  • Molecular insights into the klotho-dependent, endocrine mode of action of fibroblast growth factor 19 subfamily members.

    abstract::Unique among fibroblast growth factors (FGFs), FGF19, -21, and -23 act in an endocrine fashion to regulate energy, bile acid, glucose, lipid, phosphate, and vitamin D homeostasis. These FGFs require the presence of Klotho/betaKlotho in their target tissues. Here, we present the crystal structures of FGF19 alone and FG...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Goetz R,Beenken A,Ibrahimi OA,Kalinina J,Olsen SK,Eliseenkova AV,Xu C,Neubert TA,Zhang F,Linhardt RJ,Yu X,White KE,Inagaki T,Kliewer SA,Yamamoto M,Kurosu H,Ogawa Y,Kuro-o M,Lanske B,Razzaque MS,Mohammadi M

    更新日期:2007-05-01 00:00:00

  • Exchange protein activated by cyclic AMP (Epac)-mediated induction of suppressor of cytokine signaling 3 (SOCS-3) in vascular endothelial cells.

    abstract::Here, we demonstrate that elevation of intracellular cyclic AMP (cAMP) in vascular endothelial cells (ECs) by either a direct activator of adenylyl cyclase or endogenous cAMP-mobilizing G protein-coupled receptors inhibited the tyrosine phosphorylation of STAT proteins by an interleukin 6 (IL-6) receptor trans-signali...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sands WA,Woolson HD,Milne GR,Rutherford C,Palmer TM

    更新日期:2006-09-01 00:00:00

  • Immune functions in mice lacking Clnk, an SLP-76-related adaptor expressed in a subset of immune cells.

    abstract::The SLP-76 family of immune cell-specific adaptors is composed of three distinct members named SLP-76, Blnk, and Clnk. They have been implicated in the signaling pathways coupled to immunoreceptors such as the antigen receptors and Fc receptors. Previous studies using gene-targeted mice and deficient cell lines showed...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Utting O,Sedgmen BJ,Watts TH,Shi X,Rottapel R,Iulianella A,Lohnes D,Veillette A

    更新日期:2004-07-01 00:00:00

  • Involvement of nuclear import and export factors in U8 box C/D snoRNP biogenesis.

    abstract::Box C/D snoRNPs, factors essential for ribosome biogenesis, are proposed to be assembled in the nucleoplasm before localizing to the nucleolus. However, recent work demonstrated the involvement of nuclear export factors in this process, suggesting that export may take place. Here we show that there are distinct distri...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Watkins NJ,Lemm I,Lührmann R

    更新日期:2007-10-01 00:00:00

  • Overlapping specificities of base excision repair, nucleotide excision repair, recombination, and translesion synthesis pathways for DNA base damage in Saccharomyces cerevisiae.

    abstract::The removal of oxidative damage from Saccharomyces cerevisiae DNA is thought to be conducted primarily through the base excision repair pathway. The Escherichia coli endonuclease III homologs Ntg1p and Ntg2p are S. cerevisiae N-glycosylase-associated apurinic/apyrimidinic (AP) lyases that recognize a wide variety of d...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Swanson RL,Morey NJ,Doetsch PW,Jinks-Robertson S

    更新日期:1999-04-01 00:00:00

  • The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin.

    abstract::The mammalian target of rapamycin (mTOR) is a Ser/Thr (S/T) protein kinase, which controls mRNA translation initiation by modulating phosphorylation of the translational regulators PHAS-I and p70(S6K). Here we show that in vitro mTOR is able to phosphorylate these two regulators at comparable rates. Both (S/T)P sites,...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: McMahon LP,Choi KM,Lin TA,Abraham RT,Lawrence JC Jr

    更新日期:2002-11-01 00:00:00

  • Transcriptional pausing caused by NELF plays a dual role in regulating immediate-early expression of the junB gene.

    abstract::Human 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II (RNAPII) in vitro. However, the physiological roles of this negative regulation are not well understood. Here, by using a num...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Aida M,Chen Y,Nakajima K,Yamaguchi Y,Wada T,Handa H

    更新日期:2006-08-01 00:00:00