Nonsense-containing mRNAs that accumulate in the absence of a functional nonsense-mediated mRNA decay pathway are destabilized rapidly upon its restitution.

abstract：:We report in vivo analysis of histone and RNA polymerase II (pol II) occupancy at the 601 element, which functions as a strong in vitro nucleosome-positioning element and transcriptional pause site. Surprisingly, nucleosomes were not strongly positioned over the 601 element inserted either within a yeast chromosomal o...

journal_title：Molecular and cellular biology

authors： Perales R,Zhang L,Bentley D

更新日期：2011-08-01 00:00:00

abstract：:Adiponectin is a secretory protein abundantly secreted from adipocytes. It assembles into a number of different higher-order complexes. Adipocytes maintain tight control over circulating plasma levels, suggesting the existence of a complex, highly regulated biosynthetic pathway. However, the critical mediators of adip...

journal_title：Molecular and cellular biology

authors： Wang ZV,Schraw TD,Kim JY,Khan T,Rajala MW,Follenzi A,Scherer PE

更新日期：2007-05-01 00:00:00

abstract：:tRNAs in eukaryotic nuclei and organelles are synthesized as precursors lacking the 3'-terminal CCA sequence and possessing 5' (leader) and 3' (trailer) extensions. Nucleolytic cleavage of the 3' trailer and addition of CCA are therefore required for formation of functional tRNA 3' termini. Many chloroplast tRNA genes...

journal_title：Molecular and cellular biology

authors： Oommen A,Li XQ,Gegenheimer P

更新日期：1992-02-01 00:00:00

abstract：:Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor Oct3/4 is an indispensable factor in the self-renewal of ES cells. In this study, we searched for a protein that would interact with Oct3/4 in ES cells and identified an orphan nuclear hormone receptor...

journal_title：Molecular and cellular biology

authors： Sun C,Nakatake Y,Akagi T,Ura H,Matsuda T,Nishiyama A,Koide H,Ko MS,Niwa H,Yokota T

更新日期：2009-08-01 00:00:00

abstract：:The genes of the trithorax group (trxG) in Drosophila melanogaster are required to maintain the pattern of homeotic gene expression that is established early in embryogenesis by the transient expression of the segmentation genes. The precise role of each of the diverse trxG members and the functional relationships amo...

journal_title：Molecular and cellular biology

authors： Crosby MA,Miller C,Alon T,Watson KL,Verrijzer CP,Goldman-Levi R,Zak NB

更新日期：1999-02-01 00:00:00

abstract：:The cytosolic heat shock cognate 70-kDa protein (hsc70) is required for efficient import of ornithine transcarbamylase precursor (pOTC) into rat liver mitochondria (K. Terada, K. Ohtsuka, N. Imamoto, Y. Yoneda, and M. Mori, Mol. Cell. Biol. 15:3708-3713, 1995). The requirement of hsc70 for mitochondrial import of vari...

journal_title：Molecular and cellular biology

authors： Terada K,Ueda I,Ohtsuka K,Oda T,Ichiyama A,Mori M

更新日期：1996-11-01 00:00:00

abstract：:Genetic and biochemical experiments have enabled us to more clearly distinguish three genetic loci, emtA, emtB, and emtC, all of which can be altered to give rise to resistance to the protein synthesis inhibitor, emetine, in cultured Chinese hamster cells. Genetic experiments have demonstrated that, unlike the emtB lo...

journal_title：Molecular and cellular biology

authors： Chang S,Wasmuth JJ

更新日期：1983-03-01 00:00:00

abstract：:Minisatellite DNA is repetitive DNA with a repeat unit length from 15 to 100 bp. While stable during mitosis, it destabilizes during meiosis, altering both in length and in sequence composition. The basis for this instability is unknown. To investigate the factors controlling minisatellite stability, a minisatellite s...

journal_title：Molecular and cellular biology

authors： Jauert PA,Edmiston SN,Conway K,Kirkpatrick DT

更新日期：2002-02-01 00:00:00

abstract：:The punc gene, encoding a member of the neural cell adhesion molecule family expressed in the developing central nervous system, limbs, and inner ear, was identified. To extend studies of the normal expression pattern of punc and to determine its function, a mouse strain bearing a lacZ/neo insertion in a 5' coding exo...

journal_title：Molecular and cellular biology

authors： Yang W,Li C,Mansour SL

更新日期：2001-09-01 00:00:00

abstract：:Phosphorylation is believed to be one of the mechanisms by which p53 becomes activated or stabilized in response to cellular stress. Previously, p53 was shown to interact with three components of transcription factor IIH (TFIIH): excision repair cross-complementing types 2 and 3 (ERCC2 and ERCC3) and p62. This communi...

journal_title：Molecular and cellular biology

authors： Lu H,Fisher RP,Bailey P,Levine AJ

更新日期：1997-10-01 00:00:00

abstract：:Ligand-induced dimerization and transphosphorylation are thought to be important events by which receptor tyrosine kinases generate cellular signals. We have investigated the ability of signalling-defective, truncated fibroblast growth factor (FGF) receptors (FGFR-1 and FGFR-2) to block the FGF response in cells that ...

journal_title：Molecular and cellular biology

authors： Li Y,Basilico C,Mansukhani A

更新日期：1994-11-01 00:00:00

abstract：:Negative-feedback loops between transcription factors and repressors in responses to xenobiotics, oxidants, heat, hypoxia, DNA damage, and infection have been described. Although common, the function of feedback is largely unstudied. Here, we define a negative-feedback loop between the Caenorhabditis elegans detoxific...

journal_title：Molecular and cellular biology

authors： Leung CK,Wang Y,Deonarine A,Tang L,Prasse S,Choe KP

更新日期：2013-09-01 00:00:00

abstract：:Paramecium tetraurelia contains two types of nuclei, a diploid germinal micronucleus and a large transcriptionally active macronucleus. The macronuclear genome is formed from the micronuclear DNA during sexual reproduction. Previous studies have shown that the processing of the A-type variable surface protein gene dur...

journal_title：Molecular and cellular biology

authors： Scott JM,Mikami K,Leeck CL,Forney JD

更新日期：1994-04-01 00:00:00

abstract：:Mutations in the Saccharomyces cerevisiae alpha-factor receptor that lead to improved response to Saccharomyces kluyveri alpha-factor were identified and sequenced. Mutants were isolated from cells bearing randomly mutagenized receptor gene (STE2) plasmids by an in vivo screen. Five mutations lead to substitutions in ...

journal_title：Molecular and cellular biology

authors： Marsh L

更新日期：1992-09-01 00:00:00

abstract：:Special AT-rich sequence-binding protein 1 (SATB1) is a tissue-restricted genome organizer that provides a key link between DNA loop organization, chromatin modification/remodeling, and transcription factor association at matrix attachment regions (MARs). The SUMO E3 ligase PIAS1 enhances SUMO conjugation to SATB1 lys...

journal_title：Molecular and cellular biology

authors： Tan JA,Song J,Chen Y,Durrin LK

更新日期：2010-06-01 00:00:00

abstract：:SR proteins are essential for pre-mRNA splicing in vitro, act early in the splicing pathway, and can influence alternative splice site choice. Here we describe the isolation of both dominant and loss-of-function alleles of B52, the gene for a Drosophila SR protein. The allele B52ED was identified as a dominant second-...

journal_title：Molecular and cellular biology

authors： Peng X,Mount SM

更新日期：1995-11-01 00:00:00

abstract：:Biallelic expression of Igf2 is frequently seen in cancers because Igf2 functions as a survival factor. In many tumors the activation of Igf2 expression has been correlated with de novo methylation of the imprinted region. We have compared the intrinsic susceptibilities of the imprinted region of Igf2 and H19, other i...

journal_title：Molecular and cellular biology

authors： Biniszkiewicz D,Gribnau J,Ramsahoye B,Gaudet F,Eggan K,Humpherys D,Mastrangelo MA,Jun Z,Walter J,Jaenisch R

更新日期：2002-04-01 00:00:00

abstract：:With v-yes DNA as the probe, a human cDNA library made from placental RNA was screened under relaxed conditions, and DNA clones derived from a novel genetic locus, termed lyn, were obtained. Nucleotide sequencing revealed that lyn could encode a novel tyrosine kinase that was very similar to mouse T-lymphocyte-specifi...

journal_title：Molecular and cellular biology

authors： Yamanashi Y,Fukushige S,Semba K,Sukegawa J,Miyajima N,Matsubara K,Yamamoto T,Toyoshima K

更新日期：1987-01-01 00:00:00

abstract：:Neural retina leucine zipper (Nrl), a key basic motif leucine zipper (bZIP) transcription factor, modulates rod photoreceptor differentiation by activating rod-specific target genes. In searching for factors that might couple with Nrl to modulate its transcriptional activity through posttranslational modification, we ...

journal_title：Molecular and cellular biology

authors： Kim JW,Jang SM,Kim CH,An JH,Choi KH

更新日期：2012-05-01 00:00:00

abstract：:Synemin, a 230-kilodalton polypeptide component of avian muscle and erythrocyte intermediate filaments, is also found in association with the vimentin filaments of lens tissue. In chicken lens cells, synemin is bound to the core vimentin polymer with the same 180-nm periodicity that it exhibits in erythrocytes. Its so...

journal_title：Molecular and cellular biology

authors： Granger BL,Lazarides E

更新日期：1984-10-01 00:00:00

abstract：:The formation of inosine at the wobble position of eukaryotic tRNAs is an essential modification catalyzed by the ADAT2/ADAT3 complex. In humans, a valine-to-methionine mutation (V144M) in ADAT3 that originated ∼1,600 years ago is the most common cause of autosomal recessive intellectual disability (ID) in Arabia. Whi...

journal_title：Molecular and cellular biology

authors： Ramos J,Han L,Li Y,Hagelskamp F,Kellner SM,Alkuraya FS,Phizicky EM,Fu D

更新日期：2019-09-11 00:00:00

abstract：:Transcriptional activation of eukaryotic genes depends on the precise and ordered recruitment of activators, chromatin modifiers/remodelers, coactivators, and general transcription factors to the promoters of target genes. Using the human matrix metalloproteinase 9 (MMP-9) gene as a model system, we investigated the s...

journal_title：Molecular and cellular biology

authors： Ma Z,Shah RC,Chang MJ,Benveniste EN

更新日期：2004-06-01 00:00:00

abstract：:Forkhead (Fkh) transcription factors influence cell death, proliferation, and differentiation and the cell cycle. In Saccharomyces cerevisiae, Fkh2 both activates and represses transcription of CLB2, encoding a B-type cyclin. CLB2 is expressed during G(2)/M phase and repressed during G(1). Fkh2 recruits the coactivato...

journal_title：Molecular and cellular biology

authors： Sherriff JA,Kent NA,Mellor J

更新日期：2007-04-01 00:00:00

abstract：:Deacetylation of histones is carried out by a corepressor complex in which Sin3A is an essential scaffold protein. Two proteins in this complex, the Sin3A-associated proteins SAP30L and SAP30, have previously been suggested to function as linker molecules between various corepressors. In this report, we demonstrate ne...

journal_title：Molecular and cellular biology

authors： Viiri KM,Jänis J,Siggers T,Heinonen TY,Valjakka J,Bulyk ML,Mäki M,Lohi O

更新日期：2009-01-01 00:00:00

abstract：:The tissue-specific expression of immunoglobulin genes can be partially explained by a requirement for activating factors found only in B lymphocytes and their derivatives. However, loss of immunoglobulin expression upon fusion of an immunoglobulin-producing myeloma cell with a T lymphoma cell (BW5147) or fibroblast (...

journal_title：Molecular and cellular biology

authors： Zaller DM,Yu H,Eckhardt LA

更新日期：1988-05-01 00:00:00

abstract：:Diminished cyclooxygenase 2 (COX-2) expression in fibroblasts, with a resultant defect in the production of the antifibrotic mediator prostaglandin E(2), plays a key role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). Here, we have characterized the molecular mechanism. We found that COX-2 mRNA levels in ...

journal_title：Molecular and cellular biology

authors： Coward WR,Watts K,Feghali-Bostwick CA,Knox A,Pang L

更新日期：2009-08-01 00:00:00

abstract：:3'-end processing of nucleus-encoded mRNAs includes the addition of a poly(A) tail that is important for translation initiation. Since the vast majority of chloroplast mRNAs acquire their 3' termini by processing yet are not polyadenylated, we asked whether 3' end maturation plays a role in chloroplast translation. A ...

journal_title：Molecular and cellular biology

authors： Rott R,Levy H,Drager RG,Stern DB,Schuster G

更新日期：1998-08-01 00:00:00

abstract：:GATA1 is a master regulator of erythropoiesis, expression of which is regulated by multiple discrete cis-acting elements. In this study, we examine the activity of a promoter-proximal double GATA (dbGATA) motif, using a Gata1 bacterial artificial chromosome (BAC)-transgenic green fluorescent protein (GFP) reporter (G1...

journal_title：Molecular and cellular biology

authors： Moriguchi T,Suzuki M,Yu L,Takai J,Ohneda K,Yamamoto M

更新日期：2015-03-01 00:00:00

abstract：:Cell fate specification requires precise coordination of transcription factors and their regulators to achieve fidelity and flexibility in lineage allocation. The transcriptional repressor growth factor independence 1 (GFI1) is comprised of conserved Snail/Slug/Gfi1 (SNAG) and zinc finger motifs separated by a linker ...

journal_title：Molecular and cellular biology

authors： Andrade D,Velinder M,Singer J,Maese L,Bareyan D,Nguyen H,Chandrasekharan MB,Lucente H,McClellan D,Jones D,Sharma S,Liu F,Engel ME

更新日期：2016-05-02 00:00:00

abstract：:It is thought that degradation of nuclear proteins by the ubiquitylation system requires nuclear-cytoplasmic trafficking of E3 ubiquitin ligases. The von Hippel-Lindau (VHL) tumor suppressor protein is the substrate recognition component of a Cullin-2-containing E3 ubiquitin ligase that recruits hypoxia-inducible fact...

journal_title：Molecular and cellular biology

authors： Khacho M,Mekhail K,Pilon-Larose K,Payette J,Lee S

更新日期：2008-01-01 00:00:00