SUMOylation Regulates Growth Factor Independence 1 in Transcriptional Control and Hematopoiesis.

abstract：:A special group of mitochondrial outer membrane proteins spans the membrane once, exposing soluble domains to both sides of the membrane. These proteins are synthesized in the cytosol and then inserted into the membrane by an unknown mechanism. To identify proteins that are involved in the biogenesis of the single-spa...

journal_title：Molecular and cellular biology

authors： Papić D,Elbaz-Alon Y,Koerdt SN,Leopold K,Worm D,Jung M,Schuldiner M,Rapaport D

更新日期：2013-10-01 00:00:00

abstract：:To determine the types of alterations in gene structure induced by DNA-alkylating agents, we analyzed the restriction enzyme cleavage patterns of adenine phosphoribosyltransferase gene sequences in mutant strains of Chinese hamster ovary cells deficient in this enzyme. Base pair changes as detected by loss of restrict...

journal_title：Molecular and cellular biology

authors： Meuth M,Arrand JE

更新日期：1982-11-01 00:00:00

abstract：:We examined the relationship between pre-mRNA splicing and the nuclear matrix by using an in vivo system that we have developed. Plasmids containing the inducible herpesvirus tk gene promoter linked to an intron-containing segment of the rabbit beta-globin gene were transfected into HeLa cells, and then the promoter w...

journal_title：Molecular and cellular biology

authors： Zeitlin S,Parent A,Silverstein S,Efstratiadis A

更新日期：1987-01-01 00:00:00

abstract：:The Saccharomyces cerevisiae PGK (phosphoglycerate kinase) gene encodes one of the most abundant mRNA and protein species in the cell. To identify the promoter sequences required for the efficient expression of PGK, we undertook a detailed internal deletion analysis of the 5' noncoding region of the gene. Our analysis...

journal_title：Molecular and cellular biology

authors： Ogden JE,Stanway C,Kim S,Mellor J,Kingsman AJ,Kingsman SM

更新日期：1986-12-01 00:00:00

abstract：:Transcriptome profiling studies have recently uncovered a large number of noncoding RNA transcripts (ncRNAs) in eukaryotic organisms, and there is growing interest in their role in the cell. For example, in haploid Saccharomyces cerevisiae cells, the expression of an overlapping antisense ncRNA, referred to here as RM...

journal_title：Molecular and cellular biology

authors： Gelfand B,Mead J,Bruning A,Apostolopoulos N,Tadigotla V,Nagaraj V,Sengupta AM,Vershon AK

更新日期：2011-04-01 00:00:00

abstract：:The erythropoietin (Epo) gene is under tissue-specific inducible regulation. Because the kidney is the primary EPO-producing tissue in adults, impaired EPO production in chronic kidney disorders results in serious renal anemia. The Epo gene contains a liver-specific enhancer in the 3' region, but the kidney-specific e...

journal_title：Molecular and cellular biology

authors： Hirano I,Suzuki N,Yamazaki S,Sekine H,Minegishi N,Shimizu R,Yamamoto M

更新日期：2017-02-01 00:00:00

abstract：:Polycomb group proteins mediate heritable transcriptional silencing and function through multiprotein complexes that methylate and ubiquitinate histones. The 600-kDa E(Z)/ESC complex, also known as Polycomb repressive complex 2 (PRC2), specifically methylates histone H3 lysine 27 (H3 K27) through the intrinsic histone...

journal_title：Molecular and cellular biology

authors： Tie F,Stratton CA,Kurzhals RL,Harte PJ

更新日期：2007-03-01 00:00:00

abstract：:We isolated a Drosophila fickleP (ficP) mutant with a shortened copulatory duration and reduced adult-stage life span. The reduced copulatory duration is ascribable to incomplete fusion of the left and right halves of the apodeme that holds the penis during copulation. ficP is an intronic mutation occurring in the Btk...

journal_title：Molecular and cellular biology

authors： Baba K,Takeshita A,Majima K,Ueda R,Kondo S,Juni N,Yamamoto D

更新日期：1999-06-01 00:00:00

abstract：:Cellular senescence is an irreversible growth arrest and is presumed to be a natural barrier to tumor development. Like telomere shortening, certain defects in chromosome integrity can trigger senescence; however, the roles of centromere proteins in regulating commitment to the senescent state remains to be establishe...

journal_title：Molecular and cellular biology

authors： Maehara K,Takahashi K,Saitoh S

更新日期：2010-05-01 00:00:00

abstract：:The Ras-RAF-mitogen-activated protein kinase (Ras-RAF-MAPK) pathway is overactive in many cancers and in some developmental disorders. In one of those disorders, namely, Noonan syndrome, nine activating C-RAF mutations cluster around Ser(259), a regulatory site for inhibition by 14-3-3 proteins. We show that these mut...

journal_title：Molecular and cellular biology

authors： Molzan M,Schumacher B,Ottmann C,Baljuls A,Polzien L,Weyand M,Thiel P,Rose R,Rose M,Kuhenne P,Kaiser M,Rapp UR,Kuhlmann J,Ottmann C

更新日期：2010-10-01 00:00:00

abstract：:The c-Myc oncoprotein (Myc) controls cell fate by regulating gene transcription in association with a DNA-binding partner, Max. While Max lacks a transcription regulatory domain, the N terminus of Myc contains a transcription activation domain (TAD) that recruits cofactor complexes containing the histone acetyltransfe...

journal_title：Molecular and cellular biology

authors： Faiola F,Liu X,Lo S,Pan S,Zhang K,Lymar E,Farina A,Martinez E

更新日期：2005-12-01 00:00:00

abstract：:One of the central questions in eukaryotic transcription is how activators can transmit their signal to stimulate gene expression in the context of chromatin. The multisubunit SAGA coactivator complex has both histone acetyltransferase and deubiquitination activities and remodels chromatin to allow transcription. Whet...

journal_title：Molecular and cellular biology

authors： Nagy Z,Riss A,Romier C,le Guezennec X,Dongre AR,Orpinell M,Han J,Stunnenberg H,Tora L

更新日期：2009-03-01 00:00:00

abstract：:Although the U1 small nuclear ribonucleoprotein particle (snRNP) was the first mRNA-splicing cofactor to be identified, the manner in which it functions in splicing is not precisely understood. Among the information required to understand how U1 snRNP participates in splicing, it will be necessary to know its structur...

journal_title：Molecular and cellular biology

authors： Patton JR,Patterson RJ,Pederson T

更新日期：1987-11-01 00:00:00

abstract：:The two beta-tubulin genes of the unicellular green alga Chlamydomonas reinhardtii are expressed coordinately after deflagellation and produce two transcripts of 2.1 and 2.0 kilobases. Full-length cDNA clones corresponding to the transcript of each gene were isolated. DNA sequences were obtained from the cDNA clones a...

journal_title：Molecular and cellular biology

authors： Youngblom J,Schloss JA,Silflow CD

更新日期：1984-12-01 00:00:00

abstract：:We reported the identification of a new family of DNA-binding proteins from our characterization of the dead ringer (dri) gene of Drosophila melanogaster. We show that dri encodes a nuclear protein that contains a sequence-specific DNA-binding domain that bears no similarity to known DNA-binding domains. A number of p...

journal_title：Molecular and cellular biology

authors： Gregory SL,Kortschak RD,Kalionis B,Saint R

更新日期：1996-03-01 00:00:00

abstract：:The conserved RNA binding protein La recognizes UUU-3'OH on its small nuclear RNA ligands and stabilizes them against 3'-end-mediated decay. We report that newly described La-related protein 4 (LARP4) is a factor that can bind poly(A) RNA and interact with poly(A) binding protein (PABP). Yeast two-hybrid analysis and ...

journal_title：Molecular and cellular biology

authors： Yang R,Gaidamakov SA,Xie J,Lee J,Martino L,Kozlov G,Crawford AK,Russo AN,Conte MR,Gehring K,Maraia RJ

更新日期：2011-02-01 00:00:00

abstract：:Although RNA and RNA-binding proteins have been linked to double-strand breaks (DSBs), little is known regarding their roles in the cellular response to DSBs and, if any, in the repair process. Here, we provide direct evidence for the presence of RNA-DNA hybrids at DSBs and suggest that binding of RNA to DNA at DSBs m...

journal_title：Molecular and cellular biology

authors： Li L,Germain DR,Poon HY,Hildebrandt MR,Monckton EA,McDonald D,Hendzel MJ,Godbout R

更新日期：2016-10-28 00:00:00

abstract：:Determining the functions of novel genes implicated in cell survival is directly relevant to our understanding of mammalian development and carcinogenesis. ARS2 is an evolutionarily conserved gene that confers arsenite resistance on arsenite-sensitive Chinese hamster ovary cells. Little is known regarding the function...

journal_title：Molecular and cellular biology

authors： Wilson MD,Wang D,Wagner R,Breyssens H,Gertsenstein M,Lobe C,Lu X,Nagy A,Burke RD,Koop BF,Howard PL

更新日期：2008-03-01 00:00:00

abstract：:We recently identified Vav, the product of the vav proto-oncogene, as a guanine nucleotide exchange factor (GEF) for Ras. Vav is enzymatically activated by lymphocyte antigen receptor-coupled protein tyrosine kinases or independently by diglycerides. To further evaluate the physiological role of Vav, we assessed its G...

journal_title：Molecular and cellular biology

authors： Gulbins E,Coggeshall KM,Langlet C,Baier G,Bonnefoy-Berard N,Burn P,Wittinghofer A,Katzav S,Altman A

更新日期：1994-02-01 00:00:00

abstract：:The cys-3+ gene of Neurospora crassa encodes a bZIP (basic region-leucine zipper) regulatory protein that is essential for sulfur structural gene expression (e.g., ars-1+). Nuclear transcription assays confirmed that cys-3+ was under sulfur-regulated transcriptional control and that cys-3+ transcription was constituti...

journal_title：Molecular and cellular biology

authors： Paietta JV

更新日期：1992-04-01 00:00:00

abstract：:In vitro studies have implicated nuclear respiratory factor 1 (NRF-1) in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. We investigated here the in vivo function of NRF-1 in mammals by disrupting the gene in mice. A...

journal_title：Molecular and cellular biology

authors： Huo L,Scarpulla RC

更新日期：2001-01-01 00:00:00

abstract：:Cks1 is an activator of the SCF(Skp2) ubiquitin ligase complex that targets the cell cycle inhibitor p27(Kip1) for degradation. The loss of Cks1 results in p27(Kip1) accumulation and decreased proliferation and inhibits tumorigenesis. We identify here a function of Cks1 in mammalian cell cycle regulation that is indep...

journal_title：Molecular and cellular biology

authors： Hoellein A,Graf S,Bassermann F,Schoeffmann S,Platz U,Hölzlwimmer G,Kröger M,Peschel C,Oostendorp R,Quintanilla-Fend L,Keller U

更新日期：2012-07-01 00:00:00

abstract：:A 249-nucleotide coding region instability determinant (CRD) destabilizes c-myc mRNA. Previous experiments identified a CRD-binding protein (CRD-BP) that appears to protect the CRD from endonuclease cleavage. However, it was unclear why a CRD-BP is required to protect a well-translated mRNA whose coding region is cove...

journal_title：Molecular and cellular biology

authors： Lemm I,Ross J

更新日期：2002-06-01 00:00:00

abstract：:Transcriptional activators interact with multisubunit coactivators that modify chromatin structure or recruit the general transcriptional machinery to their target genes. Budding yeast cells respond to amino acid starvation by inducing an activator of amino acid biosynthetic genes, Gcn4p. We conducted a comprehensive ...

journal_title：Molecular and cellular biology

authors： Swanson MJ,Qiu H,Sumibcay L,Krueger A,Kim SJ,Natarajan K,Yoon S,Hinnebusch AG

更新日期：2003-04-01 00:00:00

abstract：:Tetraspanins are clustered in specific microdomains (named tetraspanin-enriched microdomains, or TERM) in the plasma membrane and regulate the functions of associated transmembrane receptors, including integrins and receptor tyrosine kinases. We have identified syntenin-1, a PDZ domain-containing protein, as a new com...

journal_title：Molecular and cellular biology

authors： Latysheva N,Muratov G,Rajesh S,Padgett M,Hotchin NA,Overduin M,Berditchevski F

更新日期：2006-10-01 00:00:00

abstract：:TRAF2 is an adaptor protein that regulates the activation of the c-Jun N-terminal kinase (JNK) and IkappaB kinase (IKK) signaling cascades in response to tumor necrosis factor alpha (TNF-alpha) stimulation. Although the downstream events in TNF-alpha signaling are better understood, the membrane-proximal events are st...

journal_title：Molecular and cellular biology

authors： Blackwell K,Zhang L,Thomas GS,Sun S,Nakano H,Habelhah H

更新日期：2009-01-01 00:00:00

abstract：:Protein ubiquitination plays a key role in the regulation of a variety of DNA repair mechanisms. Protein ubiquitination is controlled by the coordinate activity of ubiquitin ligases and deubiquitinating enzymes (DUBs). The deubiquitinating enzyme USP1 regulates DNA repair and the Fanconi anemia pathway through its ass...

journal_title：Molecular and cellular biology

authors： Murai J,Yang K,Dejsuphong D,Hirota K,Takeda S,D'Andrea AD

更新日期：2011-06-01 00:00:00

abstract：:At least two separate but interdependent events are required to attain autonomous growth as a consequence of ectopic expression of the multilineage colony-stimulating factor gene in hematopoietic progenitor cells. The rate at which the second event occurs is more than 3 orders of magnitude higher in precursor cell lin...

journal_title：Molecular and cellular biology

authors： Laker C,Kluge N,Stocking C,Just U,Franz MJ,Ostertag W,DeLamarter JF,Dexter M,Spooncer E

更新日期：1989-12-01 00:00:00

abstract：:Gene activation requires alteration of chromatin structure to facilitate active transcription complex formation at a gene promoter. Nucleosome remodeling complexes and histone modifying complexes each play unique and interdependent roles in bringing about these changes. The role of distant enhancers in these structura...

journal_title：Molecular and cellular biology

authors： Kim A,Dean A

更新日期：2003-11-01 00:00:00

abstract：:Hypoxia induces apoptosis but also triggers adaptive mechanisms to ensure cell survival. Here we show that the prosurvival effects of hypoxia-inducible factor 1 (HIF-1) in endothelial cells are mediated by neuron-derived orphan receptor 1 (NOR-1). The overexpression of NOR-1 decreased the rate of endothelial cells und...

journal_title：Molecular and cellular biology

authors： Martorell L,Gentile M,Rius J,Rodríguez C,Crespo J,Badimon L,Martínez-González J

更新日期：2009-11-01 00:00:00