3'-Processed mRNA is preferentially translated in Chlamydomonas reinhardtii chloroplasts.

abstract：:One of the proteins identified as being involved in ribosome biogenesis by high-throughput studies, a putative P-loop-type kinase termed Fap7 (YDL166c), was shown to be required for the conversion of 20S pre-rRNA to 18S rRNA. However, the mechanism underlying this function has remained unclear. Here we demonstrate tha...

journal_title：Molecular and cellular biology

authors： Granneman S,Nandineni MR,Baserga SJ

更新日期：2005-12-01 00:00:00

abstract：:Estrogen receptors (ERs) are hormone-regulated transcription factors that regulate key aspects of reproduction and development. ERs are unusual in that they do not typically repress transcription in the absence of hormone but instead possess otherwise cryptic repressive functions that are revealed upon binding to cert...

journal_title：Molecular and cellular biology

authors： Varlakhanova N,Snyder C,Jose S,Hahm JB,Privalsky ML

更新日期：2010-03-01 00:00:00

abstract：:Starting with hybrid cell lines between a Chinese hamster cell EmtA mutant and a Chinese hamster cell EmtB mutant, we have constructed cell lines that are homozygous for mutant alleles at both the emtA locus and the emtB locus, by using a two-step segregation protocol. The EmtA EmtB double mutants are approximately 10...

journal_title：Molecular and cellular biology

authors： Chang S,Wasmuth JJ

更新日期：1983-05-01 00:00:00

abstract：:The activities of the related Abl and Arg nonreceptor tyrosine kinases are kept under tight control in cells, but exposure to several different stimuli results in a two- to fivefold stimulation of kinase activity. Following the breakdown of inhibitory intramolecular interactions, Abl activation requires phosphorylatio...

journal_title：Molecular and cellular biology

authors： Tanis KQ,Veach D,Duewel HS,Bornmann WG,Koleske AJ

更新日期：2003-06-01 00:00:00

abstract：:Both cDNA clones and a genomic DNA clone encoding a 509-amino-acid protein that is 64% similar to chicken pp60c-src were isolated from the simple metazoan Hydra attenuata. We have designated this gene STK, for src-type kinase. Features of the amino acid sequence of the protein encoded by the STK gene suggest that it i...

journal_title：Molecular and cellular biology

authors： Bosch TC,Unger TF,Fisher DA,Steele RE

更新日期：1989-10-01 00:00:00

abstract：:The kidney's vital filtration function depends on the structural integrity of the glomerulus, the proximal portion of the nephron. Within the glomerulus, the architecturally complex podocyte forms the final cellular barrier to filtration. Injury to the podocyte results in a morphological change called foot process eff...

journal_title：Molecular and cellular biology

authors： Yu H,Suleiman H,Kim AH,Miner JH,Dani A,Shaw AS,Akilesh S

更新日期：2013-12-01 00:00:00

abstract：:CTCFL, a paralog of CTCF, also known as BORIS (brother of regulator of imprinted sites), is a testis-expressed gene whose function is largely unknown. Its product is a cancer testis antigen (CTA), and it is often expressed in tumor cells and also seen in two benign human vascular malformations, juvenile angiofibromas ...

journal_title：Molecular and cellular biology

authors： Sati L,Zeiss C,Yekkala K,Demir R,McGrath J

更新日期：2015-10-01 00:00:00

abstract：:CCAAT/enhancer-binding protein alpha (C/EBPalpha) is one of the key transcription factors that mediate lineage specification and differentiation of multipotent myeloid progenitors into mature granulocytes. Although C/EBPalpha is known to induce granulopoiesis while suppressing monocyte differentiation, it is unclear h...

journal_title：Molecular and cellular biology

authors： Ross SE,Radomska HS,Wu B,Zhang P,Winnay JN,Bajnok L,Wright WS,Schaufele F,Tenen DG,MacDougald OA

更新日期：2004-01-01 00:00:00

abstract：:alpha 1-Antitrypsin (AT), the major elastase inhibitor in mammalian serum, is produced primarily in the liver. We have characterized AT gene structure and expression in the mouse species Mus caroli, which expresses high levels of AT in the kidneys as well as in the liver. Analysis of cDNA and genomic clones showed tha...

journal_title：Molecular and cellular biology

authors： Latimer JJ,Berger FG,Baumann H

更新日期：1990-02-01 00:00:00

abstract：:The expression of chromosomally integrated transgenes usually varies greatly among independent transfectants. This variability in transgene expression has led to the definition of locus control regions (LCRs) as elements which render expression consistent. Analyses of expression in single cells revealed that the expre...

journal_title：Molecular and cellular biology

authors： Ronai D,Berru M,Shulman MJ

更新日期：1999-10-01 00:00:00

abstract：:Cells can regulate their protein repertoire in response to extracellular stimuli via alternative splicing; however, the mechanisms controlling this process are poorly understood. The CD45 gene undergoes alternative splicing in response to T-cell activation to regulate T-cell function. The ESS1 splicing silencer in CD4...

journal_title：Molecular and cellular biology

authors： Melton AA,Jackson J,Wang J,Lynch KW

更新日期：2007-10-01 00:00:00

abstract：:Prostaglandins, pleiotropic immune modulators that induce protein kinase A (PKA), inhibit gamma interferon induction of class II major histocompatibility complex (MHC) genes. We show that phosphorylation of CIITA by PKA accounts for this inhibition. Treatment with prostaglandin E or 8-bromo-cyclic AMP or transfection ...

journal_title：Molecular and cellular biology

authors： Li G,Harton JA,Zhu X,Ting JP

更新日期：2001-07-01 00:00:00

abstract：:To identify and characterize the proteins encoded by the erbA proto-oncogene, we expressed the C-terminal region of v-erbA in a bacterial trpE expression vector system and used the fusion protein to prepare antiserum. The anti-trp-erbA serum recognized the P75gag-erbA protein encoded by avian erythroblastosis virus an...

journal_title：Molecular and cellular biology

authors： Bigler J,Eisenman RN

更新日期：1988-10-01 00:00:00

abstract：:Both chemotaxis and phagocytosis depend upon actin-driven cell protrusions and cell membrane remodeling. While chemoattractant receptors rely upon canonical G-protein signaling to activate downstream effectors, whether such signaling pathways affect phagocytosis is contentious. Here, we report that Gαi nucleotide exch...

journal_title：Molecular and cellular biology

authors： Huang NN,Becker S,Boularan C,Kamenyeva O,Vural A,Hwang IY,Shi CS,Kehrl JH

更新日期：2014-11-15 00:00:00

abstract：:The recruitment of transcriptional coactivators, including histone modifying enzymes, is an important step in transcription regulation. A typical activator is thought to interact with several cofactors, presumably in a sequential manner. The common use of several cofactors raises the question of how activators achieve...

journal_title：Molecular and cellular biology

authors： Gamper AM,Roeder RG

更新日期：2008-04-01 00:00:00

abstract：:Exchange proteins activated by cAMP (cyclic AMP) 2 (Epac2) is a guanine nucleotide exchange factor for Rap1, a small G protein involved in many cellular functions, including cell adhesion, differentiation, and exocytosis. Epac2 interacts with Ras-GTP via a Ras association (RA) domain. Previous studies have suggested t...

journal_title：Molecular and cellular biology

authors： Liu C,Takahashi M,Li Y,Song S,Dillon TJ,Shinde U,Stork PJ

更新日期：2008-12-01 00:00:00

abstract：:In a previous paper, we have shown that in the absence of stress, mouse embryonal carcinoma cells, like mouse early embryo multipotent cells, synthesize high levels of 89- and 70-kilodalton heat shock proteins (HSP)(O. Bensaude and M. Morange, EMBO J. 2:173-177, 1983). We report here the pattern of proteins synthesize...

journal_title：Molecular and cellular biology

authors： Morange M,Diu A,Bensaude O,Babinet C

更新日期：1984-04-01 00:00:00

abstract：:A set of genes is rapidly inducible when quiescent fibroblasts are stimulated by growth factors or by the activation of temperature-sensitive retroviral protein-tyrosine kinases. Most of these so-called immediate-early genes were cloned by differential cDNA hybridization. DNA sequence analysis identified many of them ...

journal_title：Molecular and cellular biology

authors： Jähner D,Hunter T

更新日期：1991-07-01 00:00:00

abstract：:Two mutator genes of mammalian cells were demonstrated. One was associated with the ribonucleoside diphosphate reductase, and the other was associated with an extreme adenosine sensitivity. ...

journal_title：Molecular and cellular biology

authors： Chan VL,Guttman S,Juranka P

更新日期：1981-06-01 00:00:00

abstract：:Aflatoxin B1 (AFB1) is a human hepatotoxin and hepatocarcinogen produced by the mold Aspergillus flavus. In humans, AFB1 is primarily bioactivated by cytochrome P450 1A2 (CYP1A2) and 3A4 to a genotoxic epoxide that forms N7-guanine DNA adducts. A series of yeast haploid mutants defective in DNA repair and cell cycle c...

journal_title：Molecular and cellular biology

authors： Guo Y,Breeden LL,Zarbl H,Preston BD,Eaton DL

更新日期：2005-07-01 00:00:00

abstract：:Mitochondrial respiratory complexes of the electron transport chain (CI, CIII, and CIV) can be assembled into larger structures forming supercomplexes. We analyzed the assembly/stability of respiratory complexes in mouse lung fibroblasts lacking the Rieske iron-sulfur protein (RISP knockout [KO]cells), one of the cata...

journal_title：Molecular and cellular biology

authors： Diaz F,Enríquez JA,Moraes CT

更新日期：2012-01-01 00:00:00

abstract：:GIPC is a PDZ protein located on peripheral endosomes that binds to the juxtamembrane region of the TrkA nerve growth factor (NGF) receptor and has been implicated in NGF signaling. We establish here that endogenous GIPC binds to the C terminus of APPL, a Rab5 binding protein, which is a marker for signaling endosomes...

journal_title：Molecular and cellular biology

authors： Varsano T,Dong MQ,Niesman I,Gacula H,Lou X,Ma T,Testa JR,Yates JR 3rd,Farquhar MG

更新日期：2006-12-01 00:00:00

abstract：:Simultaneous resistance to an array of drugs with different cytotoxic activities is a property of Saccharomyces cerevisiae, in which the protein Pdr3p has recently been shown to play a role as a transcriptional regulator. We provide evidence that the yeast PDR3 gene, which encodes a zinc finger transcription factor im...

journal_title：Molecular and cellular biology

authors： Delahodde A,Delaveau T,Jacq C

更新日期：1995-08-01 00:00:00

abstract：:The expression of three different actin genes in the sea urchin, Strongylocentrotus purpuratus, was monitored in embryos and adult tissues by using untranslated mRNA sequences as specific hybridization probes. Three distinct patterns of expression were found: muscle specific, embryo specific, and constitutive (i.e., p...

journal_title：Molecular and cellular biology

authors： Garcia R,Paz-Aliaga B,Ernst SG,Crain WR Jr

更新日期：1984-05-01 00:00:00

abstract：:Wrch-1, an atypical and transforming Rho GTPase, regulates cellular activities including proliferation and actin organization, but its functions and effectors remain poorly characterized. We show here that Wrch-1 distributes along the apical and basolateral membranes in MDCK cells and binds the cell polarity protein P...

journal_title：Molecular and cellular biology

authors： Brady DC,Alan JK,Madigan JP,Fanning AS,Cox AD

更新日期：2009-02-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the SWI-SNF complex has been proposed to antagonize the repressive effects of chromatin by disrupting nucleosomes. The SIN genes were identified as suppressors of defects in the SWI-SNF complex, and the SIN1 gene encodes an HMG1-like protein that has been proposed to be a compone...

journal_title：Molecular and cellular biology

authors： Pérez-Martín J,Johnson AD

更新日期：1998-07-01 00:00:00

abstract：:Receptor tyrosine kinases (RTKs) play distinct roles in multiple biological systems. Many RTKs transmit similar signals, raising questions about how specificity is achieved. One potential mechanism for RTK specificity is control of the magnitude and kinetics of activation of downstream pathways. We have found that the...

journal_title：Molecular and cellular biology

authors： Zhang SQ,Tsiaras WG,Araki T,Wen G,Minichiello L,Klein R,Neel BG

更新日期：2002-06-01 00:00:00

abstract：:The LINE-1, or L1, family of interspersed repeated DNA constitutes roughly 10% of the mammalian genome. Its abundance is due to duplicative transposition via an RNA intermediate, L1-encoded proteins, and reverse transcription. Although, in principle, transposition may occur in any cell type, expression and transpositi...

journal_title：Molecular and cellular biology

authors： Branciforte D,Martin SL

更新日期：1994-04-01 00:00:00

abstract：:Exposure of monocytic cells to bacterial lipopolysaccharide (LPS) activates the NF-kappa B/Rel family of proteins and leads to the rapid induction of inflammatory gene products, including tissue factor (TF). TF is the primary cellular initiator of the coagulation protease cascades. Here we report the characterization ...

journal_title：Molecular and cellular biology

authors： Oeth PA,Parry GC,Kunsch C,Nantermet P,Rosen CA,Mackman N

更新日期：1994-06-01 00:00:00

abstract：:The Drosophila extra sex combs (esc) protein, a member of the Polycomb group (PcG), is a transcriptional repressor of homeotic genes. Genetic studies have shown that esc protein is required in early embryos at about the time that other PcG proteins become engaged in homeotic gene repression. The esc protein consists p...

journal_title：Molecular and cellular biology

authors： Ng J,Li R,Morgan K,Simon J

更新日期：1997-11-01 00:00:00