Expression of the CTCFL Gene during Mouse Embryogenesis Causes Growth Retardation, Postnatal Lethality, and Dysregulation of the Transforming Growth Factor β Pathway.

abstract：:Numerous nonribosomal trans-acting factors involved in pre-rRNA processing have been characterized, but few of them are specifically required for the last cytoplasmic steps of 18S rRNA maturation. We have recently demonstrated that Rrp10p/Rio1p is such a factor. By BLAST analysis, we identified the product of a previo...

journal_title：Molecular and cellular biology

authors： Vanrobays E,Gelugne JP,Gleizes PE,Caizergues-Ferrer M

更新日期：2003-03-01 00:00:00

abstract：:Alkylpurine-DNA-N-glycosylase (APNG) null mice have been generated by homologous recombination in embryonic stem cells. The null status of the animals was confirmed at the mRNA level by reverse transcription-PCR and by the inability of cell extracts of tissues from the knockout (ko) animals to release 3-methyladenine ...

journal_title：Molecular and cellular biology

authors： Elder RH,Jansen JG,Weeks RJ,Willington MA,Deans B,Watson AJ,Mynett KJ,Bailey JA,Cooper DP,Rafferty JA,Heeran MC,Wijnhoven SW,van Zeeland AA,Margison GP

更新日期：1998-10-01 00:00:00

abstract：:A fragment of the human gene for c-erb-B was used to map homologous sequences in mice. Analysis of somatic cell hybrids and recombinant inbred and congenic mouse strains indicated that this gene, designated Erbb, is closely linked to the gene for alpha-globin on mouse chromosome 11. Several genes controlling hematopoi...

journal_title：Molecular and cellular biology

authors： Silver J,Whitney JB 3rd,Kozak C,Hollis G,Kirsch I

更新日期：1985-07-01 00:00:00

abstract：:Neutrophils are first responders rapidly mobilized to inflammatory sites by a tightly regulated, nonredundant hierarchy of chemoattractants. These chemoattractants engage neutrophil cell surface receptors triggering heterotrimeric G-protein Gα(i) subunits to exchange GDP for GTP. By limiting the duration that Gα(i) su...

journal_title：Molecular and cellular biology

authors： Cho H,Kamenyeva O,Yung S,Gao JL,Hwang IY,Park C,Murphy PM,Neubig RR,Kehrl JH

更新日期：2012-11-01 00:00:00

abstract：:We have previously described a transcription unit on human chromosome 8, designated as PVT, that is consistently disrupted by the minority forms of translocations [t(2;8) and t(8;22)] in Burkitt's lymphoma. PVT begins 57 kilobase pairs downstream of the proto-oncogene MYC and is more than 200 kilobase pairs in length....

journal_title：Molecular and cellular biology

authors： Shtivelman E,Bishop JM

更新日期：1990-04-01 00:00:00

abstract：:The carcinoembryonic antigen (CEA) family consists of a large group of evolutionarily divergent glycoproteins. The secreted pregnancy-specific glycoproteins constitute a subgroup within the CEA family. They are predominantly expressed in trophoblast cells throughout placental development and are essential for a positi...

journal_title：Molecular and cellular biology

authors： Finkenzeller D,Fischer B,McLaughlin J,Schrewe H,Ledermann B,Zimmermann W

更新日期：2000-10-01 00:00:00

abstract：:The differentiation of dental epithelia into enamel-producing ameloblasts or the root epithelial lineage compartmentalizes teeth into crowns and roots. Bmp signaling has been linked to enamel formation, but its role in root epithelial lineage differentiation is unclear. Here we show that cessation of epithelial Bmp si...

journal_title：Molecular and cellular biology

authors： Yang Z,Hai B,Qin L,Ti X,Shangguan L,Zhao Y,Wiggins L,Liu Y,Feng JQ,Chang JY,Wang F,Liu F

更新日期：2013-12-01 00:00:00

abstract：:Incubation of WEHI 231 cells, derived from a murine B-cell lymphoma, with antisera directed against its surface immunoglobulin results in the inhibition of growth within 24 h. Previously, we demonstrated that this treatment selectively affects cytoplasmic levels of c-myc mRNA (J. E. McCormack, V. H. Pepe, R. B. Kent, ...

journal_title：Molecular and cellular biology

authors： Levine RA,McCormack JE,Buckler A,Sonenshein GE

更新日期：1986-11-01 00:00:00

abstract：:We have investigated the mechanism underlying repression of calcitonin/calcitonin gene-related peptide (CT/CGRP) gene expression by retinoic acid. Retinoic acid treatment of the CA77 thyroid C-cell line decreased CT/CGRP promoter activity two- to threefold, which correlates well with the decrease in calcitonin and CGR...

journal_title：Molecular and cellular biology

authors： Lanigan TM,Tverberg LA,Russo AF

更新日期：1993-10-01 00:00:00

abstract：:The erect wing (ewg) locus of Drosophila melanogaster encodes a vital function important for the development of the nervous system and the indirect flight muscles. In order to understand the ewg function at a molecular level, cDNA clones were isolated. Sequence analysis of cDNAs revealed a single open reading frame (O...

journal_title：Molecular and cellular biology

authors： DeSimone SM,White K

更新日期：1993-06-01 00:00:00

abstract：:Methylation of histone H3 on lysine 9 or 27 is crucial for heterochromatin formation. Previously considered hallmarks of, respectively, constitutive and facultative heterochromatin, recent evidence has accumulated in favor of coexistence of these two marks and their cooperation in gene silencing maintenance. H3K9me2/3...

journal_title：Molecular and cellular biology

authors： Boros J,Arnoult N,Stroobant V,Collet JF,Decottignies A

更新日期：2014-10-01 00:00:00

abstract：:CCAAT/enhancer-binding protein alpha (C/EBPalpha) is one of the key transcription factors that mediate lineage specification and differentiation of multipotent myeloid progenitors into mature granulocytes. Although C/EBPalpha is known to induce granulopoiesis while suppressing monocyte differentiation, it is unclear h...

journal_title：Molecular and cellular biology

authors： Ross SE,Radomska HS,Wu B,Zhang P,Winnay JN,Bajnok L,Wright WS,Schaufele F,Tenen DG,MacDougald OA

更新日期：2004-01-01 00:00:00

abstract：:Protein kinase activities were measured in Rous sarcoma virus-infected baby hamster kidney (BHK) cells that express v-src (BHK [v-src]) and compared with those of revertant and control BHK cells. We observed about a fivefold-higher tyrosine phosphorylating activity in BHK (v-src) cell extracts, which was due to src bu...

journal_title：Molecular and cellular biology

authors： Clinton GM,Roskoski R Jr

更新日期：1984-05-01 00:00:00

abstract：:We have developed a high-frequency cotransformation system which is useful in introducing nonreplicating circular DNA plasmids into the fission yeast Schizosaccharomyces pombe. This system depends on two factors: the ability of the ural-complementing helper plasmids pFYM2 and pFYM225 to propagate autonomously in S. po...

journal_title：Molecular and cellular biology

authors： Sakai K,Sakaguchi J,Yamamoto M

更新日期：1984-04-01 00:00:00

abstract：:Two peroxins of the AAA family, PpPex1p and PpPex6p, are required for peroxisome biogenesis in the yeast Pichia pastoris. Cells from the corresponding deletion strains (Pp delta pex1 and Pp delta pex6) contain only small vesicular remnants of peroxisomes, the bulk of peroxisomal matrix proteins is mislocalized to the ...

journal_title：Molecular and cellular biology

authors： Faber KN,Heyman JA,Subramani S

更新日期：1998-02-01 00:00:00

abstract：:Precisely regulated expression of oncogenes and tumor suppressor genes is essential for normal development, and deregulated expression can lead to cancer. The human N-myc gene normally is expressed in only a subset of fetal epithelial tissues, and its expression is extinguished in all adult tissues except transiently ...

journal_title：Molecular and cellular biology

authors： Sivak LE,Pont-Kingdon G,Le K,Mayr G,Tai KF,Stevens BT,Carroll WL

更新日期：1999-01-01 00:00:00

abstract：:Activating mutations in KEAP1-NRF2 are frequently found in tumors of the lung, esophagus, and liver, where they are associated with aggressive growth, resistance to cancer therapies, and low overall survival. Despite the fact that NRF2 is a validated driver of tumorigenesis and chemotherapeutic resistance, there are c...

journal_title：Molecular and cellular biology

authors： Baird L,Suzuki T,Takahashi Y,Hishinuma E,Saigusa D,Yamamoto M

更新日期：2020-10-26 00:00:00

abstract：:The Ras-RAF-mitogen-activated protein kinase (Ras-RAF-MAPK) pathway is overactive in many cancers and in some developmental disorders. In one of those disorders, namely, Noonan syndrome, nine activating C-RAF mutations cluster around Ser(259), a regulatory site for inhibition by 14-3-3 proteins. We show that these mut...

journal_title：Molecular and cellular biology

authors： Molzan M,Schumacher B,Ottmann C,Baljuls A,Polzien L,Weyand M,Thiel P,Rose R,Rose M,Kuhenne P,Kaiser M,Rapp UR,Kuhlmann J,Ottmann C

更新日期：2010-10-01 00:00:00

abstract：:Saccharomyces cerevisiae dbf4 and cdc7 cell cycle mutants block initiation of DNA synthesis (i.e., are iDS mutants) at 37 degrees C and arrest the cell cycle with a 1C DNA content. Surprisingly, certain dbf4 and cdc7 strains divide their chromatin at 37 degrees C. We found that the activation of the Cdc28 mitotic prot...

journal_title：Molecular and cellular biology

authors： Toyn JH,Johnson AL,Johnston LH

更新日期：1995-10-01 00:00:00

abstract：:Genomic DNA in higher eucaryotic cells is organized into a series of loops, each of which may be affixed at its base to the nuclear matrix via a specific matrix attachment region (MAR). In this report, we describe the distribution of MARs within the amplified dihydrofolate reductase (DHFR) domain (amplicon) in the met...

journal_title：Molecular and cellular biology

authors： Dijkwel PA,Hamlin JL

更新日期：1988-12-01 00:00:00

abstract：:Gap junctions are membrane channels that permit the interchange of ions and other low-molecular-weight molecules between adjacent cells. Rous sarcoma virus (RSV)-induced transformation is marked by an early and profound disruption of gap-junctional communication, suggesting that these membrane structures may serve as ...

journal_title：Molecular and cellular biology

authors： Crow DS,Beyer EC,Paul DL,Kobe SS,Lau AF

更新日期：1990-04-01 00:00:00

abstract：:The Epstein-Barr virus BRLF1 and BZLF1 genes are the first viral genes transcribed upon induction of the viral lytic cycle. The protein products of both genes (referred to here as Rta and Zta, respectively) activate expression of other viral genes, thereby initiating the lytic cascade. Among the viral antigens express...

journal_title：Molecular and cellular biology

authors： Flemington EK,Lytle JP,Cayrol C,Borras AM,Speck SH

更新日期：1994-05-01 00:00:00

abstract：:We have examined the ability of the simian virus 40 72-base pair enhancer segment to simultaneously activate multiple transcription units with plasmids that contain one, two, or three simian virus 40-based transcription units in various arrangements. After transfection into CV1 cells, the expression of a marker gene, ...

journal_title：Molecular and cellular biology

authors： Kadesch T,Berg P

更新日期：1986-07-01 00:00:00

abstract：:Transcription from the Moloney murine leukemia virus (Mo-MuLV) long terminal repeat (LTR) is inhibited in murine stem cells and induced during maturation of these cells. We have investigated whether alterations in the activity of this viral regulatory element also occur during differentiation of human myeloid leukemia...

journal_title：Molecular and cellular biology

authors： Reisman D,Rotter V

更新日期：1989-08-01 00:00:00

abstract：:Alpha-catenin, an integral part of cadherin-catenin adhesion complexes, is a major binding partner of beta-catenin, a key component of the Wnt pathway, which activates T-cell factor (TCF)/lymphoid enhancer factor (LEF) transcription and is often upregulated in cancers. Recently, we identified an alpha-catenin-related ...

journal_title：Molecular and cellular biology

authors： Merdek KD,Nguyen NT,Toksoz D

更新日期：2004-03-01 00:00:00

abstract：:The retinoblastoma binding protein 1 (RBP1) appears to be an important factor in the repression of E2F-dependent transcription by the retinoblastoma protein (pRB) family. The recent identification of the breast carcinoma associated antigen (BCAA) as an RBP1-like protein led us to investigate its biological properties ...

journal_title：Molecular and cellular biology

authors： Binda O,Roy JS,Branton PE

更新日期：2006-03-01 00:00:00

abstract：:Mutations in polycystin 2 (PC2), a Ca(2+)-permeable cation channel, cause autosomal dominant polycystic kidney disease. Whether PC2 functions in the endoplasmic reticulum (ER) or in the plasma membrane has been controversial. Here we generated and characterized a polyclonal antibody against PC2, determined the subcell...

journal_title：Molecular and cellular biology

authors： Luo Y,Vassilev PM,Li X,Kawanabe Y,Zhou J

更新日期：2003-04-01 00:00:00

abstract：:Endonuclease G (endo G) is one of the most abundant nucleases in eukaryotic cells. It is encoded in the nucleus and imported to the mitochondrial intermembrane space. This nuclease is active on single- and double-stranded DNA. We genetically disrupted the endo G gene in mice without disturbing a conserved, overlapping...

journal_title：Molecular and cellular biology

authors： Irvine RA,Adachi N,Shibata DK,Cassell GD,Yu K,Karanjawala ZE,Hsieh CL,Lieber MR

更新日期：2005-01-01 00:00:00

abstract：:We describe here a new cell line, EL2, which spontaneously arose from primary rat embryo fibroblasts and has the distinctive property of being highly susceptible to a number of different transforming genes. The high susceptibility is expressed not only in high transformation frequencies but, most importantly, in an un...

journal_title：Molecular and cellular biology

authors： Liboi E,Caruso M,Basilico C

更新日期：1984-12-01 00:00:00

abstract：:We have designed and constructed integration-defective retroviral vectors to explore their potential for gene targeting in mammalian cells. Two nonoverlapping deletion mutants of the bacterial neomycin resistance (neo) gene were used to detect homologous recombination events between viral and chromosomal sequences. St...

journal_title：Molecular and cellular biology

authors： Ellis J,Bernstein A

更新日期：1989-04-01 00:00:00