Abstract:
:Protein kinase activities were measured in Rous sarcoma virus-infected baby hamster kidney (BHK) cells that express v-src (BHK [v-src]) and compared with those of revertant and control BHK cells. We observed about a fivefold-higher tyrosine phosphorylating activity in BHK (v-src) cell extracts, which was due to src but not other cellular tyrosyl kinase activities since preincubation with anti-src serum reduced the activity to control cell levels. The cyclic AMP-dependent protein kinase activity was also altered when v-src was expressed. Resolution of the two cyclic AMP-dependent isozymes from the detergent-soluble fraction of cells revealed that the type I activity was selectively decreased about fivefold in BHK (v-src) cells.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Clinton GM,Roskoski R Jrdoi
10.1128/mcb.4.5.973subject
Has Abstractpub_date
1984-05-01 00:00:00pages
973-7issue
5eissn
0270-7306issn
1098-5549journal_volume
4pub_type
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