A novel intron element operates posttranscriptionally To regulate human N-myc expression.

abstract：:We analyzed epsilon-globin transcription in erythroid cells and in erythroid extracts to determine the requirements for enhancer-dependent expression of this gene. Mutations that abolished GATA-1 binding at a single position in the promoter prevented interaction with enhancers, whereas elimination of a second more dis...

journal_title：Molecular and cellular biology

authors： Gong Q,Dean A

更新日期：1993-02-01 00:00:00

abstract：:The E1a gene of adenovirus encodes two proteins, 289 and 243 amino acids long, which have positive (transactivator) and negative (enhancer repressor) RNA polymerase II transcriptional regulatory properties and cell transformation activities including cooperation with an activated ras gene. The E1a transforming functio...

journal_title：Molecular and cellular biology

authors： Velcich A,Ziff E

更新日期：1988-05-01 00:00:00

abstract：:Here, we demonstrate that SENP2, a desumoylating enzyme, plays a critical role in the control of adipogenesis. SENP2 expression was markedly increased upon the induction of adipocyte differentiation, and this increase was dependent on protein kinase A activation. Remarkably, knockdown of SENP2 led to a dramatic attenu...

journal_title：Molecular and cellular biology

authors： Chung SS,Ahn BY,Kim M,Choi HH,Park HS,Kang S,Park SG,Kim YB,Cho YM,Lee HK,Chung CH,Park KS

更新日期：2010-05-01 00:00:00

abstract：:Multiple biological functions have been ascribed to the Ras-related G protein R-Ras. These include the ability to transform NIH 3T3 fibroblasts, the promotion of cell adhesion, and the regulation of apoptotic responses in hematopoietic cells. To investigate the signaling mechanisms responsible for these biological phe...

journal_title：Molecular and cellular biology

authors： Osada M,Tolkacheva T,Li W,Chan TO,Tsichlis PN,Saez R,Kimmelman AC,Chan AM

更新日期：1999-09-01 00:00:00

abstract：:With v-yes DNA as the probe, a human cDNA library made from placental RNA was screened under relaxed conditions, and DNA clones derived from a novel genetic locus, termed lyn, were obtained. Nucleotide sequencing revealed that lyn could encode a novel tyrosine kinase that was very similar to mouse T-lymphocyte-specifi...

journal_title：Molecular and cellular biology

authors： Yamanashi Y,Fukushige S,Semba K,Sukegawa J,Miyajima N,Matsubara K,Yamamoto T,Toyoshima K

更新日期：1987-01-01 00:00:00

abstract：:Avian leukosis virus (ALV)-induced neoplasias are commonly found associated with integrations of proviral DNA in proximity to the myc gene. However, studies suggest that other genetic events are necessary for the complete neoplastic phenotype. A cell line (HP46) derived from an ALV-induced tumor has been analyzed and ...

journal_title：Molecular and cellular biology

authors： Kabrun N,Bumstead N,Hayman MJ,Enrietto PJ

更新日期：1990-09-01 00:00:00

abstract：:Addition of serum to mitogen-starved cells activates the cellular immediate-early gene (IEG) response. Serum response factor (SRF) contributes to such mitogen-stimulated transcriptional induction of many IEGs during the G0-G1 cell cycle transition. SRF is also believed to be essential for cell cycle progression, as im...

journal_title：Molecular and cellular biology

authors： Schratt G,Weinhold B,Lundberg AS,Schuck S,Berger J,Schwarz H,Weinberg RA,Rüther U,Nordheim A

更新日期：2001-04-01 00:00:00

abstract：:Costimulation of G protein-coupled receptors (GPCRs) may result in cross talk interactions between their downstream signaling pathways. Stimulation of GPCRs may also lead to cross talk regulation of receptor tyrosine kinase signaling and thereby to activation of mitogen-activated protein kinase (MAPK). In COS-7 cells,...

journal_title：Molecular and cellular biology

authors： Hanke S,Nürnberg B,Groll DH,Liebmann C

更新日期：2001-12-01 00:00:00

abstract：:Setd8 is the sole histone methyltransferase in mammals capable of monomethylating histone H4 lysine 20 (H4K20me1). Setd8 is expressed at significantly higher levels in erythroid cells than any other cell or tissue type, suggesting that Setd8 has an erythroid-cell-specific function. To test this hypothesis, stable Setd...

journal_title：Molecular and cellular biology

authors： Malik J,Getman M,Steiner LA

更新日期：2015-06-01 00:00:00

abstract：:Posttranscriptional regulation is important for tumor necrosis factor alpha (TNF-alpha) expression in monocytes and macrophages, and an AU-rich element (ARE) in the 3' untranslated region (UTR) of TNF-alpha mRNA is implicated in control of its translation and mRNA stability. Regulation of mRNA turnover is thought to b...

journal_title：Molecular and cellular biology

authors： Dean JL,Wait R,Mahtani KR,Sully G,Clark AR,Saklatvala J

更新日期：2001-02-01 00:00:00

abstract：:The eukaryotic Spt4-Spt5 heterodimer forms a higher-order complex with RNA polymerase II (and I) to regulate transcription elongation. Extensive genetic and functional data have revealed diverse roles of Spt4-Spt5 in coupling elongation with chromatin modification and RNA-processing pathways. A mechanistic understandi...

journal_title：Molecular and cellular biology

authors： Meyer PA,Li S,Zhang M,Yamada K,Takagi Y,Hartzog GA,Fu J

更新日期：2015-10-01 00:00:00

abstract：:Rats and mice have two, equally expressed, nonallelic genes encoding preproinsulin (genes I and II). Cytological hybridization with metaphase chromosomes indicated that both genes reside on rat chromosome I but are approximately 100,000 kilobases apart. In mice the two genes reside on two different chromosomes. DNA se...

journal_title：Molecular and cellular biology

authors： Soares MB,Schon E,Henderson A,Karathanasis SK,Cate R,Zeitlin S,Chirgwin J,Efstratiadis A

更新日期：1985-08-01 00:00:00

abstract：:The biosynthesis of influenza virus hemagglutinin (HA) and its translocation across microsomal membranes were studied in a mammalian cell-free system. All forms of HA could be cotranslationally translocated with high efficiency. However, only truncated forms of HA were translocated after protein synthesis has been ter...

journal_title：Molecular and cellular biology

authors： Chao CC,Bird P,Gething MJ,Sambrook J

更新日期：1987-10-01 00:00:00

abstract：:Chromosomal rearrangements associated with one Ty1 element in the iso-1-cytochrome c (CYC1) region of Saccharomyces cerevisiae yeast cells were examined. Most of the rearrangements were deletions of the three linked genes, CYC1, OSM1, and RAD7, and resulted from recombination involving the single Ty1 element and a sol...

journal_title：Molecular and cellular biology

authors： Downs KM,Brennan G,Liebman SW

更新日期：1985-12-01 00:00:00

abstract：:Homologous recombination is now routinely used in mammalian cells to replace endogenous chromosomal sequences with transferred DNA. Vectors for this purpose are traditionally constructed so that the replacement segment is flanked on both sides by DNA sequences which are identical to sequences in the chromosomal target...

journal_title：Molecular and cellular biology

authors： Berinstein N,Pennell N,Ottaway CA,Shulman MJ

更新日期：1992-01-01 00:00:00

abstract：:The tissue-specific expression of immunoglobulin genes can be partially explained by a requirement for activating factors found only in B lymphocytes and their derivatives. However, loss of immunoglobulin expression upon fusion of an immunoglobulin-producing myeloma cell with a T lymphoma cell (BW5147) or fibroblast (...

journal_title：Molecular and cellular biology

authors： Zaller DM,Yu H,Eckhardt LA

更新日期：1988-05-01 00:00:00

abstract：:Transcriptional insulators are cis regulatory elements that organize chromatin into independently regulated domains. At the imprinted murine Igf2/H19 locus, the H19-ICR insulator prevents the activation of the Igf2 promoter on the maternal allele by enhancers that activate H19 on the same chromosome. Given the well-de...

journal_title：Molecular and cellular biology

authors： Singh V,Srivastava M

更新日期：2008-06-01 00:00:00

abstract：:The onc gene (v-fes) of the acutely transforming feline sarcoma virus (Snyder-Theilen strain) has homologous cellular sequences (c-fes) in all vertebrate species, including humans. We isolated from a human DNA library recombinant phages containing overlapping c-fes sequences. The human c-fes locus spans a region of 3....

journal_title：Molecular and cellular biology

authors： Franchini G,Gelmann EP,Dalla-Favera R,Gallo RC,Wong-Staal F

更新日期：1982-08-01 00:00:00

abstract：:Compromised immunoregulation contributes to obesity and complications in metabolic pathogenesis. Here, we demonstrate that the nuclear factor of activated T cell (NFAT) group of transcription factors contributes to glucose and insulin homeostasis. Expression of two members of the NFAT family (NFATc2 and NFATc4) is ind...

journal_title：Molecular and cellular biology

authors： Yang TT,Suk HY,Yang X,Olabisi O,Yu RY,Durand J,Jelicks LA,Kim JY,Scherer PE,Wang Y,Feng Y,Rossetti L,Graef IA,Crabtree GR,Chow CW

更新日期：2006-10-01 00:00:00

abstract：:Cellular senescence is a tumor-suppressive process characterized by an irreversible cell cycle exit, a unique morphology, and expression of senescence-associated beta-galactosidase (SA-beta-Gal). We report here a role for CDK5 in induction of senescent cytoskeletal changes. CDK5 activation is upregulated in senescing ...

journal_title：Molecular and cellular biology

authors： Alexander K,Yang HS,Hinds PW

更新日期：2004-04-01 00:00:00

abstract：:A novel small nuclear ribonucleoprotein (snRNP) complex containing both U11 and U12 RNAs has been identified in HeLa cell extracts. This U11/U12 snRNP complex can be visualized on glycerol gradients, on native polyacrylamide gels, and by selection with antisense 2'-O-methyl oligoribonucleotides. RNase H-mediated degra...

journal_title：Molecular and cellular biology

authors： Wassarman KM,Steitz JA

更新日期：1992-03-01 00:00:00

abstract：:Recent results have shown that the ability of farnesyltransferase inhibitors (FTIs) to inhibit malignant cell transformation and Ras prenylation can be separated. We proposed previously that farnesylated Rho proteins are important targets for alternation by FTIs, based on studies of RhoB (the FTI-Rho hypothesis). Cell...

journal_title：Molecular and cellular biology

authors： Du W,Lebowitz PF,Prendergast GC

更新日期：1999-03-01 00:00:00

abstract：:We employed a protein gel blotting procedure to search for nuclear proteins from rat pituitary cells that bind preferentially to the 5'-flanking region of the rat prolactin gene. By gel blots of chromatin proteins from GH3 rat pituitary tumor cells with a 32P-labeled prolactin genomic clone, we detected two major bind...

journal_title：Molecular and cellular biology

authors： White BA,Preston GM,Lufkin TC,Bancroft C

更新日期：1985-11-01 00:00:00

abstract：:To identify novel genes that play critical roles in mediating bone morphogenetic protein (BMP) signal pathways, we performed a yeast two-hybrid screen using Smad1 as bait. A novel mouse Krüppel-type zinc finger protein, mZnf8, was isolated. Interactions between mZnf8 and Smad proteins were further analyzed with variou...

journal_title：Molecular and cellular biology

authors： Jiao K,Zhou Y,Hogan BL

更新日期：2002-11-01 00:00:00

abstract：:Treatment of K562 cells, a human erythroleukemia cell line, with desferrioxamine raised the levels of the receptor for transferrin (Tf) two- to threefold over that of the control cells. The levels of receptor were reduced by at least 50 and 35% of that of the control in cells treated with diferric Tf and ferric ammoni...

journal_title：Molecular and cellular biology

authors： Rao KK,Shapiro D,Mattia E,Bridges K,Klausner R

更新日期：1985-04-01 00:00:00

abstract：:Mutations in the gene encoding methyl-CpG-binding protein 2 (MeCP2) lead to disrupted neuronal function and can cause the neurodevelopmental disorder Rett syndrome. MeCP2 is a transcriptional regulator that binds to methylated DNA and is most abundant in neuronal nuclei. The mechanisms by which MeCP2 regulates gene ex...

journal_title：Molecular and cellular biology

authors： Gonzales ML,Adams S,Dunaway KW,LaSalle JM

更新日期：2012-07-01 00:00:00

abstract：:Thyroid hormone receptors are encoded by the TRalpha (NR1A1) and TRbeta (NR1A2) loci. These genes are transcribed into multiple variants whose functions are unclear. Analysis by gene inactivation in mice has provided new insights into the functional complexity of these products. Different strategies designed to modify...

journal_title：Molecular and cellular biology

authors： Gauthier K,Plateroti M,Harvey CB,Williams GR,Weiss RE,Refetoff S,Willott JF,Sundin V,Roux JP,Malaval L,Hara M,Samarut J,Chassande O

更新日期：2001-07-01 00:00:00

abstract：:Transforming Harvey (Ha) ras oncogene products accelerated the time course of Xenopus oocyte maturation induced by insulin, insulinlike growth factor 1, or progesterone. The transforming constructs, [Val-12]Ha p21 and [Val-12, Thr-59]Ha p21, displayed equal potency and efficacy in their abilities to accelerate the gro...

journal_title：Molecular and cellular biology

authors： Sadler SE,Maller JL,Gibbs JB

更新日期：1990-04-01 00:00:00

abstract：:Integration of protein kinases into transcription activation complexes influences the magnitude of gene expression. The nuclear factor of activated T cells (NFAT) group of proteins are critical transcription factors that direct gene expression in immune and nonimmune cells. A balance of phosphotransferase activity is ...

journal_title：Molecular and cellular biology

authors： Yang TT,Xiong Q,Graef IA,Crabtree GR,Chow CW

更新日期：2005-02-01 00:00:00

abstract：:Liu and Mertz (Genes Dev. 9:1766-1780, 1995) previously identified a 119-nt pre-mRNA processing enhancer (PPE) element within the herpes simplex virus type 1 thymidine kinase gene that enables intron-independent gene expression in higher eukaryotes by binding heterogeneous nuclear ribonucleoprotein L (hnRNP L). Here, ...

journal_title：Molecular and cellular biology

authors： Guang S,Felthauser AM,Mertz JE

更新日期：2005-08-01 00:00:00