Abstract:
:Addition of serum to mitogen-starved cells activates the cellular immediate-early gene (IEG) response. Serum response factor (SRF) contributes to such mitogen-stimulated transcriptional induction of many IEGs during the G0-G1 cell cycle transition. SRF is also believed to be essential for cell cycle progression, as impairment of SRF activity by specific antisera or antisense RNA has previously been shown to block mammalian cell proliferation. In contrast, Srf(-/-) mouse embryos grow and develop up to E6.0. Using the embryonic stem (ES) cell system, we demonstrate here that wild-type ES cells do not undergo complete cell cycle arrest upon serum withdrawal but that they can mount an efficient IEG response. This IEG response, however, is severely impaired in Srf(-/-) ES cells, providing the first genetic proof that IEG activation is dependent upon SRF. Also, Srf(-/-) ES cells display altered cellular morphology, reduced cortical actin expression, and an impaired plating efficiency on gelatin. Yet, despite these defects, the proliferation rates of Srf(-/-) ES cells are not substantially altered, demonstrating that SRF function is not required for ES cell cycle progression.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Schratt G,Weinhold B,Lundberg AS,Schuck S,Berger J,Schwarz H,Weinberg RA,Rüther U,Nordheim Adoi
10.1128/MCB.21.8.2933-2943.2001subject
Has Abstractpub_date
2001-04-01 00:00:00pages
2933-43issue
8eissn
0270-7306issn
1098-5549journal_volume
21pub_type
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