ATP-dependent chromatin remodeling by the Cockayne syndrome B DNA repair-transcription-coupling factor.

abstract：:Little is known about the regions of RNA polymerase II (RNAPII) that are involved in the process of transcript elongation and interaction with elongation factors. One elongation factor, TFIIS, stimulates transcript elongation by binding to RNAPII and facilitating its passage through intrinsic pausing sites in vitro. I...

journal_title：Molecular and cellular biology

authors： Archambault J,Lacroute F,Ruet A,Friesen JD

更新日期：1992-09-01 00:00:00

abstract：:Deacetylation of histones is carried out by a corepressor complex in which Sin3A is an essential scaffold protein. Two proteins in this complex, the Sin3A-associated proteins SAP30L and SAP30, have previously been suggested to function as linker molecules between various corepressors. In this report, we demonstrate ne...

journal_title：Molecular and cellular biology

authors： Viiri KM,Jänis J,Siggers T,Heinonen TY,Valjakka J,Bulyk ML,Mäki M,Lohi O

更新日期：2009-01-01 00:00:00

abstract：:CTCFL, a paralog of CTCF, also known as BORIS (brother of regulator of imprinted sites), is a testis-expressed gene whose function is largely unknown. Its product is a cancer testis antigen (CTA), and it is often expressed in tumor cells and also seen in two benign human vascular malformations, juvenile angiofibromas ...

journal_title：Molecular and cellular biology

authors： Sati L,Zeiss C,Yekkala K,Demir R,McGrath J

更新日期：2015-10-01 00:00:00

abstract：:In the budding yeast Saccharomyces cerevisiae, mutations in the essential gene CDC1 cause defects in Golgi inheritance and actin polarization. However, the biochemical function of Cdc1p is unknown. Previous work showed that cdc1 mutants accumulate intracellular Ca(2+) and display enhanced sensitivity to the extracellu...

journal_title：Molecular and cellular biology

authors： Losev E,Papanikou E,Rossanese OW,Glick BS

更新日期：2008-05-01 00:00:00

abstract：:After flagellar detachment in Chlamydomonas reinhardi, there is a rapid synthesis and accumulation of mRNAs for tubulin and other flagellar proteins. Maximum levels of these mRNAs (flagellar RNAs) are reached within 1 h after deflagellation, after which they are rapidly degraded to their predeflagellation levels. The ...

journal_title：Molecular and cellular biology

authors： Baker EJ,Keller LR,Schloss JA,Rosenbaum JL

更新日期：1986-01-01 00:00:00

abstract：:Ty1 retrotransposons in the yeast Saccharomyces cerevisiae are maintained in a genetically competent but transpositionally dormant state. When located in the ribosomal DNA (rDNA) locus, Ty1 elements are transcriptionally silenced by the specialized heterochromatin that inhibits rDNA repeat recombination. In addition, ...

journal_title：Molecular and cellular biology

authors： Bryk M,Banerjee M,Conte D Jr,Curcio MJ

更新日期：2001-08-01 00:00:00

abstract：:Prostaglandins, pleiotropic immune modulators that induce protein kinase A (PKA), inhibit gamma interferon induction of class II major histocompatibility complex (MHC) genes. We show that phosphorylation of CIITA by PKA accounts for this inhibition. Treatment with prostaglandin E or 8-bromo-cyclic AMP or transfection ...

journal_title：Molecular and cellular biology

authors： Li G,Harton JA,Zhu X,Ting JP

更新日期：2001-07-01 00:00:00

abstract：:T-lymphocyte stimulation requires activation of several protein kinases, including the major phorbol ester receptor protein kinase C (PKC), ultimately leading to induction of lymphokines, such as interleukin-2 (IL-2). The revelant PKC isoforms which are involved in the activation cascades of nuclear transcription fact...

journal_title：Molecular and cellular biology

authors： Baier-Bitterlich G,Uberall F,Bauer B,Fresser F,Wachter H,Grunicke H,Utermann G,Altman A,Baier G

更新日期：1996-04-01 00:00:00

abstract：:A characteristic feature of gene expression in eukaryotes is the addition of a 5'-terminal 7-methylguanine cap (m7GpppN) to nascent pre-mRNAs in the nucleus catalyzed by capping enzyme and cap methyltransferase. Small interfering RNA (siRNA) knockdown of cap methyltransferase in HeLa cells resulted in apoptosis as mea...

journal_title：Molecular and cellular biology

authors： Shafer B,Chu C,Shatkin AJ

更新日期：2005-04-01 00:00:00

abstract：:Transfection experiments with constructs containing various 5'-deleted fragments of the human lipoprotein lipase (LPL) promoter and the chloramphenicol acetyltransferase reporter gene revealed an LPL silencer element (LSE) in the region of nucleotides -225 to -81 of the LPL gene that functioned in Chinese hamster ovar...

journal_title：Molecular and cellular biology

authors： Tanuma Y,Nakabayashi H,Esumi M,Endo H

更新日期：1995-01-01 00:00:00

abstract：:Acquisition of the ability to produce gamma interferon (IFN-gamma) is a fundamental property of memory T cells and enables one subset (T helper 1 [TH1]) to deliver its effector functions. To examine regulation of IFN-gamma gene expression in a model system which recapitulates TH1 differentiation, we prepared reporter ...

journal_title：Molecular and cellular biology

authors： Aune TM,Penix LA,Rincón MR,Flavell RA

更新日期：1997-01-01 00:00:00

abstract：:The putative promoter of the variant surface glycoprotein (VSG) gene of Trypanosoma brucei was cloned into a plasmid containing the chloramphenicol acetyltransferase (CAT) gene. After electroporation into trypanosomes, this construct directed the expression of the CAT reporter gene. The essential region for promoter a...

journal_title：Molecular and cellular biology

authors： Jefferies D,Tebabi P,Pays E

更新日期：1991-01-01 00:00:00

abstract：:Using a sensitive transfection-tumorigenicity assay, we have isolated a novel transforming gene from the DNA of two patients with chronic myelogenous leukemia. Sequence analysis indicates that the product of this gene, axl, is a receptor tyrosine kinase. Overexpression of axl cDNA in NIH 3T3 cells induces neoplastic t...

journal_title：Molecular and cellular biology

authors： O'Bryan JP,Frye RA,Cogswell PC,Neubauer A,Kitch B,Prokop C,Espinosa R 3rd,Le Beau MM,Earp HS,Liu ET

更新日期：1991-10-01 00:00:00

abstract：:Six4 is a member of the Six family genes, homologues of Drosophila melanogaster sine oculis. The gene is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles. To elucidate t...

journal_title：Molecular and cellular biology

authors： Ozaki H,Watanabe Y,Takahashi K,Kitamura K,Tanaka A,Urase K,Momoi T,Sudo K,Sakagami J,Asano M,Iwakura Y,Kawakami K

更新日期：2001-05-01 00:00:00

abstract：:CREB binding protein (CBP) is a 270-kDa nuclear protein required for activated transcription of a large number of cellular genes. Although CBP was originally discovered through its interaction with phosphorylated CREB (pCREB), it is utilized by a multitude of cellular transcription factors and viral oncoproteins. Both...

journal_title：Molecular and cellular biology

authors： Giebler HA,Lemasson I,Nyborg JK

更新日期：2000-07-01 00:00:00

abstract：:The major histocompatibility complex class II (MHC-II) locus includes a dense cluster of genes that function to initiate immune responses. Expression of insulator CCCTC binding factor (CTCF) was found to be required for expression of all MHC class II genes associated with antigen presentation. Ten CTCF sites that divi...

journal_title：Molecular and cellular biology

authors： Majumder P,Boss JM

更新日期：2010-09-01 00:00:00

abstract：:Genetic and biochemical evidence implicates chromatin structure in the silencing of the two quiescent mating-type loci near the telomeres of chromosome III in yeast. With high-resolution micrococcal nuclease mapping, we show that the HMRa locus has 12 precisely positioned nucleosomes spanning the distance between the ...

journal_title：Molecular and cellular biology

authors： Ravindra A,Weiss K,Simpson RT

更新日期：1999-12-01 00:00:00

abstract：:A sequence-specific DNA-binding protein from skeletal-muscle extracts that binds to probes of three muscle gene DNA elements is identified. This protein, referred to as muscle factor 3, forms the predominant nucleoprotein complex with the MCAT gene sequence motif in an electrophoretic mobility shift assay. This protei...

journal_title：Molecular and cellular biology

authors： Santoro IM,Yi TM,Walsh K

更新日期：1991-04-01 00:00:00

abstract：:A series of simian virus 40 (SV40)-immortalized hepatocyte cell lines were characterized for albumin production, the regulation of albumin production, and the expression of other liver-specific genes. This series of cell lines is particularly useful for studying the regulation of hepatocyte gene expression because the...

journal_title：Molecular and cellular biology

authors： Woodworth CD,Isom HC

更新日期：1987-10-01 00:00:00

abstract：:Nearly half of human cancers harbor p53 mutations, which can promote cancerous growth, metastasis, and resistance to therapy. The gain of function of mutant p53 is partly mediated by its ability to form a complex with NF-Y or p63/p73. Here, we demonstrate that TopBP1 mediates these activities in cancer, and we provide...

journal_title：Molecular and cellular biology

authors： Liu K,Ling S,Lin WC

更新日期：2011-11-01 00:00:00

abstract：:A mixed-oligonucleotide probe was used to identify four ras-like coding sequences in a human teratocarcinoma cDNA library. Two of these sequences resembled the rho genes, one was closely related to H-, K-, and N-ras, and one shared only the four sequence domains that define the ras gene superfamily. Homologs of the fo...

journal_title：Molecular and cellular biology

authors： Drivas GT,Shih A,Coutavas E,Rush MG,D'Eustachio P

更新日期：1990-04-01 00:00:00

abstract：:In Aspergillus nidulans, a fungus that possesses negligible, if any, levels of methylation in its genome, low concentrations of 5-azacytidine (5-AC) convert a high percentage of the cell population to fluffy phenotypic variants through a heritable modification of a single nuclear gene (M. Tamame, F. Antequera, J. R. V...

journal_title：Molecular and cellular biology

authors： Tamame M,Antequera F,Santos E

更新日期：1988-08-01 00:00:00

abstract：:Yeast cells are keenly sensitive to the availability and quality of nutrients. Addition of glucose to cells growing on a poorer carbon source elicits a cell cycle delay during G1 phase and a concomitant increase in the cell size. The signal is transduced through the RAS-cyclic AMP pathway. Using synchronized populatio...

journal_title：Molecular and cellular biology

authors： Flick K,Chapman-Shimshoni D,Stuart D,Guaderrama M,Wittenberg C

更新日期：1998-05-01 00:00:00

abstract：:The homeobox gene HOXA9 has recently been shown to be an important regulator of endothelial cell (EC) differentiation and activation in addition to its role in embryonic development and hematopoiesis. In this report, we have determined that the EC-leukocyte adhesion molecule E-selectin is a key target for HOXA9. The d...

journal_title：Molecular and cellular biology

authors： Bandyopadhyay S,Ashraf MZ,Daher P,Howe PH,DiCorleto PE

更新日期：2007-06-01 00:00:00

abstract：:We have isolated a yeast conditional mutant which rapidly ceases synthesis of mRNA when subjected to the nonpermissive temperature. This mutant (rpb1-1) was constructed by replacing the wild-type chromosomal copy of the gene encoding the largest subunit of RNA polymerase II with one mutagenized in vitro. The rapid ces...

journal_title：Molecular and cellular biology

authors： Nonet M,Scafe C,Sexton J,Young R

更新日期：1987-05-01 00:00:00

abstract：:An RNA editing mechanism modifies apolipoprotein B (apo-B) mRNA in the intestine by converting cytosine at nucleotide (nt) 6666 to uracil. To define the sequence requirements for editing, mutant apo-B RNAs were analyzed for the ability to be edited in vitro by enterocyte extracts. Editing was detected by a sensitive a...

journal_title：Molecular and cellular biology

authors： Driscoll DM,Lakhe-Reddy S,Oleksa LM,Martinez D

更新日期：1993-12-01 00:00:00

abstract：:A new member of a family of site-specific retrotransposons is described in the New World trypanosome Trypanosoma cruzi. This element, CZAR (cruzi-associated retrotransposon), resembles two previously described retrotransposons found in the African trypanosome T. brucei gambiense and the mosquito trypanosomatid Crithid...

journal_title：Molecular and cellular biology

authors： Villanueva MS,Williams SP,Beard CB,Richards FF,Aksoy S

更新日期：1991-12-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the Wee1 family kinase Swe1p is normally stable during G(1) and S phases but is unstable during G(2) and M phases due to ubiquitination and subsequent degradation. However, perturbations of the actin cytoskeleton lead to a stabilization and accumulation of Swe1p. This response constitutes ...

journal_title：Molecular and cellular biology

authors： McMillan JN,Longtine MS,Sia RA,Theesfeld CL,Bardes ES,Pringle JR,Lew DJ

更新日期：1999-10-01 00:00:00

abstract：:Aberrant activation of the Wnt/β-catenin pathway and polo-like kinase 1 (Plk1) overexpression represent two common events in prostate cancer with relevant functional implications. This minireview analyzes their potential therapeutic significance in prostate cancer based on their role as androgen receptor (AR) signalin...

journal_title：Molecular and cellular biology

authors： Cristóbal I,Rojo F,Madoz-Gúrpide J,García-Foncillas J

更新日期：2016-05-31 00:00:00

abstract：:Among the tumor necrosis factor (TNF) family of cytokines, FasL and TNF-related apoptosis-inducing ligand (TRAIL) are known to induce cell death via caspase activation. Recently, other biological functions of these death ligands have been postulated in vitro and in vivo. It was previously shown that Fas ligation induc...

journal_title：Molecular and cellular biology

authors： Choi C,Kutsch O,Park J,Zhou T,Seol DW,Benveniste EN

更新日期：2002-02-01 00:00:00