Abstract:
:Genetic and biochemical evidence implicates chromatin structure in the silencing of the two quiescent mating-type loci near the telomeres of chromosome III in yeast. With high-resolution micrococcal nuclease mapping, we show that the HMRa locus has 12 precisely positioned nucleosomes spanning the distance between the E and I silencer elements. The nucleosomes are arranged in pairs with very short linkers; the pairs are separated from one another by longer linkers of approximately 20 bp. Both the basic amino-terminal region of histone H4 and the silent information regulator protein Sir3p are necessary for the organized repressive chromatin structure of the silent locus. Compared to HMRa, only small differences in the availability of the TATA box are present for the promoter in the cassette at the active MATa locus. Features of the chromatin structure of this silent locus compared to the previously studied HMLalpha locus suggest differences in the mechanisms of silencing and may relate to donor selection during mating-type interconversion.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Ravindra A,Weiss K,Simpson RTdoi
10.1128/mcb.19.12.7944subject
Has Abstractpub_date
1999-12-01 00:00:00pages
7944-50issue
12eissn
0270-7306issn
1098-5549journal_volume
19pub_type
杂志文章abstract::T antigen (Tag) from simian virus 40 binds specifically to two distinct sites in the viral origin of replication and to single-stranded DNA. Analysis of the protein domain responsible for these activities revealed the following. (i) The C-terminal boundary of the origin-specific and single-strand-specific DNA-binding ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
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