PATZ1 Is a DNA Damage-Responsive Transcription Factor That Inhibits p53 Function.


:Insults to cellular health cause p53 protein accumulation, and loss of p53 function leads to tumorigenesis. Thus, p53 has to be tightly controlled. Here we report that the BTB/POZ domain transcription factor PATZ1 (MAZR), previously known for its transcriptional suppressor functions in T lymphocytes, is a crucial regulator of p53. The novel role of PATZ1 as an inhibitor of the p53 protein marks its gene as a proto-oncogene. PATZ1-deficient cells have reduced proliferative capacity, which we assessed by transcriptome sequencing (RNA-Seq) and real-time cell growth rate analysis. PATZ1 modifies the expression of p53 target genes associated with cell proliferation gene ontology terms. Moreover, PATZ1 regulates several genes involved in cellular adhesion and morphogenesis. Significantly, treatment with the DNA damage-inducing drug doxorubicin results in the loss of the PATZ1 transcription factor as p53 accumulates. We find that PATZ1 binds to p53 and inhibits p53-dependent transcription activation. We examine the mechanism of this functional inhibitory interaction and demonstrate that PATZ1 excludes p53 from DNA binding. This study documents PATZ1 as a novel player in the p53 pathway.


Mol Cell Biol


Keskin N,Deniz E,Eryilmaz J,Un M,Batur T,Ersahin T,Cetin Atalay R,Sakaguchi S,Ellmeier W,Erman B




Has Abstract


2015-05-01 00:00:00














  • Different cis-acting DNA elements control expression of the human apolipoprotein AI gene in different cell types.

    abstract::In mammals, the gene coding for apolipoprotein AI (apoAI), a protein of the plasma lipid transport system, is expressed only in the liver and the intestine. A series of plasmids containing various lengths of sequences flanking the 5' end of the human apoAI gene were constructed and assayed for transient expression aft...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sastry KN,Seedorf U,Karathanasis SK

    更新日期:1988-02-01 00:00:00

  • The endothelial cell-specific antibody PAL-E identifies a secreted form of vimentin in the blood vasculature.

    abstract::During mammalian vascular development, endothelial cells form a complex array of vessels that differ markedly in structure and function, but the molecular basis for this vascular complexity is poorly understood. Recent insights into endothelial diversity have come from the identification of molecular markers expressed...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Xu B,deWaal RM,Mor-Vaknin N,Hibbard C,Markovitz DM,Kahn ML

    更新日期:2004-10-01 00:00:00

  • Distinct subdomains of human TAFII130 are required for interactions with glutamine-rich transcriptional activators.

    abstract::TFIID is a multiprotein complex consisting of the TATA box binding protein and multiple tightly associated proteins (TAFIIs) that are required for transcription by selected activators. We previously reported cloning and partial characterization of human TAFII130 (hTAFII130). The central domain of hTAFII130 contains fo...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Saluja D,Vassallo MF,Tanese N

    更新日期:1998-10-01 00:00:00

  • TATA-binding protein and associated factors in polymerase II and polymerase III transcription.

    abstract::Transcription by RNA polymerase I (pol I), pol II, and pol III requires the TATA-binding protein (TBP). This protein functions in association with distinct TBP-associated factors (TAFs) which may specify the nature of the polymerase selected for initiation at a promoter site. In the pol III transcription system, the T...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Meyers RE,Sharp PA

    更新日期:1993-12-01 00:00:00

  • Nonsense-containing mRNAs that accumulate in the absence of a functional nonsense-mediated mRNA decay pathway are destabilized rapidly upon its restitution.

    abstract::Nonsense-mediated mRNA decay (NMD) is a conserved proofreading mechanism that protects eukaryotic cells from the potentially deleterious effects of truncated proteins. Studies of Saccharomyces cerevisiae imply that NMD is a predominantly cytoplasmic decay pathway, while studies of mammalian systems suggest that decay ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Maderazo AB,Belk JP,He F,Jacobson A

    更新日期:2003-02-01 00:00:00

  • Conserved pattern of antisense overlapping transcription in the homologous human ERCC-1 and yeast RAD10 DNA repair gene regions.

    abstract::We report that the genes for the homologous Saccharomyces cerevisiae RAD10 and human ERCC-1 DNA excision repair proteins harbor overlapping antisense transcription units in their 3' regions. Since naturally occurring antisense transcription is rare in S. cerevisiae and humans (this is the first example in human cells)...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: van Duin M,van Den Tol J,Hoeijmakers JH,Bootsma D,Rupp IP,Reynolds P,Prakash L,Prakash S

    更新日期:1989-04-01 00:00:00

  • Germ line transcripts are processed by a Dicer-like protein that is essential for developmentally programmed genome rearrangements of Tetrahymena thermophila.

    abstract::Abundant approximately 28-nucleotide RNAs that are thought to direct histone H3 lysine 9 (H3K9) methylation and promote the elimination of nearly 15 Mbp of DNA from the developing somatic genome are generated during Tetrahymena thermophila conjugation. To identify the protein(s) that generates these small RNAs, we stu...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Malone CD,Anderson AM,Motl JA,Rexer CH,Chalker DL

    更新日期:2005-10-01 00:00:00

  • Ligand-induced repression of the glucocorticoid receptor gene is mediated by an NCoR1 repression complex formed by long-range chromatin interactions with intragenic glucocorticoid response elements.

    abstract::Glucocorticoids are among the most potent and effective agents for treating inflammatory diseases and hematological cancers. However, subpopulations of patients are often resistant to steroid therapy, and determining the molecular mechanisms that contribute to glucocorticoid resistance is thus critical to addressing t...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ramamoorthy S,Cidlowski JA

    更新日期:2013-05-01 00:00:00

  • A 12-base-pair sequence is an essential element of the ribosomal gene terminator in Xenopus laevis.

    abstract::rRNA transcription in Xenopus laevis terminates near a 7-base-pair (bp) conserved sequence (T3 box) located 200 bp upstream of the site of transcription initiation for the adjacent gene promoter. We present evidence here that a 12-bp element containing the T3 box is an essential part of the terminator. Using an oocyte...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Labhart P,Reeder RH

    更新日期:1987-05-01 00:00:00

  • A membrane-proximal region of the interleukin-2 receptor gamma c chain sufficient for Jak kinase activation and induction of proliferation in T cells.

    abstract::The interleukin-2 (IL-2) receptor (IL-2R) consists of three distinct subunits (alpha, beta, and gamma c) and regulates proliferation of T lymphocytes. Intracellular signalling results from ligand-mediated heterodimerization of the cytoplasmic domains of the beta and gamma c chains. To identify the residues of gamma c ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Nelson BH,Lord JD,Greenberg PD

    更新日期:1996-01-01 00:00:00

  • Negative regulation contributes to tissue specificity of the immunoglobulin heavy-chain enhancer.

    abstract::We have identified in and around the immunoglobulin heavy-chain enhancer two apparently distinct negative regulatory elements which repress immunoglobulin H enhancer, simian virus 40 enhancer, and heterologous promoter activity in fibroblasts but not in myeloma cells. We propose that in nonlymphoid cells, negative reg...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Imler JL,Lemaire C,Wasylyk C,Wasylyk B

    更新日期:1987-07-01 00:00:00

  • Analysis of a mutant histone H3 that perturbs the association of Swi/Snf with chromatin.

    abstract::We have isolated new histone H3 mutants in Saccharomyces cerevisiae that confer phenotypes indicative of transcriptional defects. Here we describe the characterization of one such mutant, encoded by the hht2-11 allele, which contains the single amino acid change L61W in the globular domain of H3. Whole-genome expressi...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Duina AA,Winston F

    更新日期:2004-01-01 00:00:00

  • 5'-flanking sequence required for regulated expression of a muscle-specific Drosophila melanogaster actin gene.

    abstract::We have functionally tested derivatives of a muscle-specific Drosophila melanogaster actin gene in which 5'-flanking sequences have been deleted or rearranged. From our results we conclude that approximately 1,000 nucleotides of 5'-flanking sequence are required for wild-type levels of mRNA accumulation during flight ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Geyer PK,Fyrberg EA

    更新日期:1986-10-01 00:00:00

  • Triptolide-Assisted Phosphorylation of p53 Suppresses Inflammation-Induced NF-κB Survival Pathways in Cancer Cells.

    abstract::Chronic inflammation plays important roles in cancer initiation and progression. Resolving chronic inflammation or blocking inflammatory signal transduction may prevent cancer development. Here, we report that the combined low-dose use of two anti-inflammatory drugs, aspirin and triptolide, reduces spontaneous lung ca...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zheng L,Jia J,Dai H,Wan L,Liu J,Hu L,Zhou M,Qiu M,Chen X,Chang L,Kim JY,Reckamp K,Raz DJ,Xia Z,Shen B

    更新日期:2017-07-14 00:00:00

  • Overproduction of Rb protein after the G1/S boundary causes G2 arrest.

    abstract::The Rb protein is known to exert its activity at decision points in the G1 phase of the cell cycle. To investigate whether it may also play some role(s) at later points in the cell cycle, we used a system of rapid inducible gene amplification to conditionally overexpress Rb protein during G2 phase. A cell line express...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Karantza V,Maroo A,Fay D,Sedivy JM

    更新日期:1993-11-01 00:00:00

  • Inhibition of chicken embryo lens differentiation and lens junction formation in culture by pp60v-src.

    abstract::A culture system was developed which permitted the differentiation of chicken lens epithelial cells to lentoid bodies which contained several cell layers, accumulated high levels of delta-crystallin, and produced extensive gap junctions. This differentiation process was prevented when the cells were infected with a te...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Menko AS,Boettiger D

    更新日期:1988-04-01 00:00:00

  • SSN20 is an essential gene with mutant alleles that suppress defects in SUC2 transcription in Saccharomyces cerevisiae.

    abstract::Dominant and recessive mutations at the SSN20 locus were previously isolated as extragenic suppressors of mutations in three genes (SNF2, SNF5, and SNF6) that are required in trans to derepress invertase expression. All ssn20 alleles cause recessive, temperature-sensitive lethality. In this study we cloned the SSN20 g...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Neigeborn L,Celenza JL,Carlson M

    更新日期:1987-02-01 00:00:00

  • The human SPT20-containing SAGA complex plays a direct role in the regulation of endoplasmic reticulum stress-induced genes.

    abstract::One of the central questions in eukaryotic transcription is how activators can transmit their signal to stimulate gene expression in the context of chromatin. The multisubunit SAGA coactivator complex has both histone acetyltransferase and deubiquitination activities and remodels chromatin to allow transcription. Whet...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Nagy Z,Riss A,Romier C,le Guezennec X,Dongre AR,Orpinell M,Han J,Stunnenberg H,Tora L

    更新日期:2009-03-01 00:00:00

  • Dimerization of MLH1 and PMS2 limits nuclear localization of MutLalpha.

    abstract::DNA mismatch repair maintains genomic stability by detecting and correcting mispaired DNA sequences and by signaling cell death when DNA repair fails. The mechanism by which mismatch repair coordinates DNA damage and repair with cell survival or death is not understood, but it suggests the need for regulation. Since t...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Wu X,Platt JL,Cascalho M

    更新日期:2003-05-01 00:00:00

  • The capable ABL: what is its biological function?

    abstract::The mammalian ABL1 gene encodes the ubiquitously expressed nonreceptor tyrosine kinase ABL. In response to growth factors, cytokines, cell adhesion, DNA damage, oxidative stress, and other signals, ABL is activated to stimulate cell proliferation or differentiation, survival or death, retraction, or migration. ABL als...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章,评审


    authors: Wang JY

    更新日期:2014-04-01 00:00:00

  • RIP1 Cleavage in the Kinase Domain Regulates TRAIL-Induced NF-κB Activation and Lymphoma Survival.

    abstract::Although TRAIL is considered a potential anticancer agent, it enhances tumor progression by activating NF-κB in apoptosis-resistant cells. Cellular FLICE-like inhibitory protein (cFLIP) overexpression and caspase-8 activation have been implicated in TRAIL-induced NF-κB activation; however, the underlying mechanisms ar...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zhang L,Blackwell K,Workman LM,Chen S,Pope MR,Janz S,Habelhah H

    更新日期:2015-10-01 00:00:00

  • Role of Gab1 in UV-induced c-Jun NH2-terminal kinase activation and cell apoptosis.

    abstract::Exposure of mammalian cells to UV irradiation leads to activation of the c-Jun NH(2)-terminal protein kinase (JNK) pathway, which is associated with cell apoptosis. However, the molecular mechanism for JNK activation by UV exposure is not fully understood. We show here an essential role of a multisubstrate adapter, Ga...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sun Y,Yuan J,Liu H,Shi Z,Baker K,Vuori K,Wu J,Feng GS

    更新日期:2004-02-01 00:00:00

  • Multiple proteins interact with the fushi tarazu proximal enhancer.

    abstract::The expression of the Drosophila segmentation gene fushi tarazu (ftz) is controlled at the level of transcription. The proximal enhancer, located approximately 3.4 kb upstream of the transcription start site, directs lacZ fusion gene expression in a ftz-like seven-stripe pattern in transgenic fly embryos. We have take...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Han W,Yu Y,Altan N,Pick L

    更新日期:1993-09-01 00:00:00

  • Genetic demonstration of a redundant role of extracellular signal-regulated kinase 1 (ERK1) and ERK2 mitogen-activated protein kinases in promoting fibroblast proliferation.

    abstract::The extracellular signal-regulated kinase 1 and 2 (ERK1/2) mitogen-activated protein (MAP) kinase signaling pathway plays an important role in the proliferative response of mammalian cells to mitogens. However, the individual contribution of the isoforms ERK1 and ERK2 to cell proliferation control is unclear. The two ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Voisin L,Saba-El-Leil MK,Julien C,Frémin C,Meloche S

    更新日期:2010-06-01 00:00:00

  • Induction of apoptosis by human Nbk/Bik, a BH3-containing protein that interacts with E1B 19K.

    abstract::The E1B 19-kilodalton protein (19K protein) is a potent apoptosis inhibitor and the adenovirus homolog of Bcl-2 (E. White, Genes Dev. 10:1-15, 1996). To obtain a better understanding of the biochemical mechanism by which the E1B 19K protein regulates apoptosis, proteins that interact with 19K have been identified; one...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Han J,Sabbatini P,White E

    更新日期:1996-10-01 00:00:00

  • Segregation of unreplicated chromosomes in Saccharomyces cerevisiae reveals a novel G1/M-phase checkpoint.

    abstract::Saccharomyces cerevisiae dbf4 and cdc7 cell cycle mutants block initiation of DNA synthesis (i.e., are iDS mutants) at 37 degrees C and arrest the cell cycle with a 1C DNA content. Surprisingly, certain dbf4 and cdc7 strains divide their chromatin at 37 degrees C. We found that the activation of the Cdc28 mitotic prot...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Toyn JH,Johnson AL,Johnston LH

    更新日期:1995-10-01 00:00:00

  • Genetic effects of methyl benzimidazole-2-yl-carbamate on Saccharomyces cerevisiae.

    abstract::The genetic effects of the mitotic inhibitor methyl benzimidazole-2-yl-carbamate (MBC) have been studied in Saccharomyces cerevisiae. MBC had little or no effect on the frequency of mutation. In some experiments MBC caused an increase in the frequency of mitotic recombination; however, this effect was small and not re...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Wood JS

    更新日期:1982-09-01 00:00:00

  • pp39mos is associated with p34cdc2 kinase in c-mosxe-transformed NIH 3T3 cells.

    abstract::We investigated the possible interactions between pp39mos and p34cdc2 kinase in NIH 3T3 cells transformed by c-mosxe. pp39mos is coprecipitated with p34cdc2 when using either anti-PSTAIR antibody or p13suc1-Sepharose beads. Likewise, p34cdc2 is coprecipitated with pp39mos when using anti-mos antibody. However, pp39mos...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zhou R,Daar I,Ferris DK,White G,Paules RS,Vande Woude G

    更新日期:1992-08-01 00:00:00

  • Characterization of BCE-1, a transcriptional enhancer regulated by prolactin and extracellular matrix and modulated by the state of histone acetylation.

    abstract::We have previously described a 160-bp enhancer (BCE-1) in the bovine beta-casein gene that is activated in the presence of prolactin and extracellular matrix (ECM). Here we report the characterization of the enhancer by deletion and site-directed mutagenesis, electrophoretic mobility shift analysis, and in vivo footpr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Myers CA,Schmidhauser C,Mellentin-Michelotti J,Fragoso G,Roskelley CD,Casperson G,Mossi R,Pujuguet P,Hager G,Bissell MJ

    更新日期:1998-04-01 00:00:00

  • Identification of candidate angiogenic inhibitors processed by matrix metalloproteinase 2 (MMP-2) in cell-based proteomic screens: disruption of vascular endothelial growth factor (VEGF)/heparin affin regulatory peptide (pleiotrophin) and VEGF/Connective

    abstract::Matrix metalloproteinases (MMPs) exert both pro- and antiangiogenic functions by the release of cytokines or proteolytically generated angiogenic inhibitors from extracellular matrix and basement membrane remodeling. In the Mmp2-/- mouse neovascularization is greatly reduced, but the mechanistic aspects of this remain...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Dean RA,Butler GS,Hamma-Kourbali Y,Delbé J,Brigstock DR,Courty J,Overall CM

    更新日期:2007-12-01 00:00:00