Genetic interaction between transcription elongation factor TFIIS and RNA polymerase II.

abstract：:Colon carcinomas appear to arise from the cumulative effect of mutations to several genes (APC, DCC, p53, ras, hMLH1, and hMSH2). By using novel colonic epithelial cell lines derived from the Immorto mouse, named the YAMC (young adult mouse colon) cell line, and an Immorto-Min mouse hybrid, named the IMCE (Immorto-Min...

journal_title：Molecular and cellular biology

authors： D'Abaco GM,Whitehead RH,Burgess AW

更新日期：1996-03-01 00:00:00

abstract：:The v-vis gene encodes p28sis, the transforming protein of simian sarcoma virus. This gene resulted from a fusion of the env gene of simian sarcoma-associated virus and the woolly monkey gene for the B chain of platelet-derived growth factor (PDGF). Previous work has shown that the v-sis gene product undergoes signal ...

journal_title：Molecular and cellular biology

authors： Lee BA,Maher DW,Hannink M,Donoghue DJ

更新日期：1987-10-01 00:00:00

abstract：:The nuclear enzyme DNA topoisomerase II catalyzes the breakage and resealing of duplex DNA and plays an important role in several genetic processes. It also mediates the DNA cleavage activity and cytotoxicity of clinically important anticancer agents such as etoposide. We have examined the activity of topoisomerase II...

journal_title：Molecular and cellular biology

authors： Chow KC,Ross WE

更新日期：1987-09-01 00:00:00

abstract：:We describe experiments which demonstrated that the simian virus 40 (SV40) enhancer affects certain transcriptional units differently. We also found that a specific enhancer-transcriptional unit interaction can be regulated by trans-acting factors. Using transient assays, we examined the effects of the SV40 enhancer o...

journal_title：Molecular and cellular biology

authors： Robbins PD,Rio DC,Botchan MR

更新日期：1986-04-01 00:00:00

abstract：:With v-yes DNA as the probe, a human cDNA library made from placental RNA was screened under relaxed conditions, and DNA clones derived from a novel genetic locus, termed lyn, were obtained. Nucleotide sequencing revealed that lyn could encode a novel tyrosine kinase that was very similar to mouse T-lymphocyte-specifi...

journal_title：Molecular and cellular biology

authors： Yamanashi Y,Fukushige S,Semba K,Sukegawa J,Miyajima N,Matsubara K,Yamamoto T,Toyoshima K

更新日期：1987-01-01 00:00:00

abstract：:The product of the EUG1 gene of Saccharomyces cerevisiae is a soluble endoplasmic reticulum protein with homology to both the mammalian protein disulfide isomerase (PDI) and the yeast PDI homolog encoded by the essential PDI1 gene. Deletion or overexpression of EUG1 causes no growth defects under a variety of conditio...

journal_title：Molecular and cellular biology

authors： Tachibana C,Stevens TH

更新日期：1992-10-01 00:00:00

abstract：:The mammalian ABL1 gene encodes the ubiquitously expressed nonreceptor tyrosine kinase ABL. In response to growth factors, cytokines, cell adhesion, DNA damage, oxidative stress, and other signals, ABL is activated to stimulate cell proliferation or differentiation, survival or death, retraction, or migration. ABL als...

journal_title：Molecular and cellular biology

authors： Wang JY

更新日期：2014-04-01 00:00:00

abstract：:GATA-1, a transcription factor essential for the development of the erythroid lineage, contains two adjacent highly conserved zinc finger motifs. The carboxy-terminal finger is necessary and sufficient for specific binding to the consensus GATA recognition sequence: mutant proteins containing only the amino-terminal f...

journal_title：Molecular and cellular biology

authors： Trainor CD,Omichinski JG,Vandergon TL,Gronenborn AM,Clore GM,Felsenfeld G

更新日期：1996-05-01 00:00:00

abstract：:TIN2 is an important regulator of telomere length, and mutations in TINF2, the gene encoding TIN2, cause short-telomere syndromes. While the genetics underscore the importance of TIN2, the mechanism through which TIN2 regulates telomere length remains unclear. Here, we tested the effects of human TIN2 on telomerase ac...

journal_title：Molecular and cellular biology

authors： Pike AM,Strong MA,Ouyang JPT,Greider CW

更新日期：2019-10-11 00:00:00

abstract：:Previous studies have shown that expansion-prone repeats form structures that inhibit human flap endonuclease (FEN-1). We report here that faulty processing by FEN-1 initiates repeat instability in mammalian cells. Disease-length CAG tracts in Huntington's disease mice heterozygous for FEN-1 display a tendency toward ...

journal_title：Molecular and cellular biology

authors： Spiro C,McMurray CT

更新日期：2003-09-01 00:00:00

abstract：:The transcription factor family of nuclear factor I (NFI) proteins is encoded by four closely related genes: Nfia, Nfib, Nfic, and Nfix. A potential role for NFI proteins in regulating developmental processes has been implicated by their specific expression pattern during embryonic development and by analysis of NFI-d...

journal_title：Molecular and cellular biology

authors： Driller K,Pagenstecher A,Uhl M,Omran H,Berlis A,Gründer A,Sippel AE

更新日期：2007-05-01 00:00:00

abstract：:In both tissue sections and cell culture, the endothelial nature of a cell is most commonly determined by demonstration of its expression of von Willebrand factor (vWf) protein and/or mRNA. Thus, the mechanism of cell-type-specific transcriptional regulation of the vWf gene is central to studying the basis of endothel...

journal_title：Molecular and cellular biology

authors： Jahroudi N,Lynch DC

更新日期：1994-02-01 00:00:00

abstract：:Histone acetylation and deacetylation have been implicated in the regulation of gene expression. Molecular studies have shown that histone deacetylases (HDACs) function as transcriptional repressors. However, very little is known about their roles during development in multicellular organisms. We previously demonstrat...

journal_title：Molecular and cellular biology

authors： Dufourcq P,Victor M,Gay F,Calvo D,Hodgkin J,Shi Y

更新日期：2002-05-01 00:00:00

abstract：:In the "canonical" view of transforming growth factor beta (TGF-beta) signaling, Smad7 plays an inhibitory role. While Smad7 represses Smad3 activation by TGF-beta, it does not reverse the inhibitory effect of TGF-beta on myogenesis, suggesting a different function in myogenic cells. We previously reported a promyogen...

journal_title：Molecular and cellular biology

authors： Miyake T,Alli NS,McDermott JC

更新日期：2010-02-01 00:00:00

abstract：:A protein that binds specifically to Saccharomyces cerevisiae centromere DNA element I was purified on the basis of a nitrocellulose filter-binding assay. This protein, termed centromere-binding protein 1 (CP1), was heat stable and renaturable from sodium dodecyl sulfate (SDS), and assays of eluates from SDS gels indi...

journal_title：Molecular and cellular biology

authors： Bram RJ,Kornberg RD

更新日期：1987-01-01 00:00:00

abstract：:The establishment of epigenetic marks, such as methylation on histone tails, is mechanistically linked to RNA polymerase II within active genes. To explore the interplay between these modifications in transcribed noncoding as well as coding sequences, we analyzed epigenetic modification and chromatin structure at high...

journal_title：Molecular and cellular biology

authors： Kim A,Kiefer CM,Dean A

更新日期：2007-02-01 00:00:00

abstract：:The CDC8 gene, whose product is required for DNA replication in Saccharomyces cerevisiae, has been isolated on recombinant plasmids. The yeast vector YCp50 bearing the yeast ARS1, CEN4, and URA3 sequences, to provide for replication, stability, and selection, respectively, was used to prepare a recombinant plasmid poo...

journal_title：Molecular and cellular biology

authors： Kuo CL,Campbell JL

更新日期：1983-10-01 00:00:00

abstract：:To examine the internal organization of the promoter of the Xenopus laevis rRNA gene, we constructed a series of linker-scanning mutants that traverse the rDNA initiation region. The mutant genes, which have 3 to 11 clustered base substitutions set within an otherwise unaltered rDNA promoter sequence, were injected in...

journal_title：Molecular and cellular biology

authors： Windle JJ,Sollner-Webb B

更新日期：1986-12-01 00:00:00

abstract：:Cell numbers are regulated by a balance among proliferation, growth arrest, and programmed cell death. A profound example of cell homeostasis, controlled throughout life, is the complex process of blood cell development, yet little is understood about the intracellular mechanisms that regulate blood cell growth arrest...

journal_title：Molecular and cellular biology

authors： Selvakumaran M,Lin HK,Sjin RT,Reed JC,Liebermann DA,Hoffman B

更新日期：1994-04-01 00:00:00

abstract：:We report a negative feedback loop between the signaling protein phospholipase D (PLD), phosphatidic acid (PA), and a specific set of microRNAs (miRNAs) during nutrient starvation of breast cancer cells. We show that PLD expression is increased in four breast cancer cell lines and that hypoxia, cell overcrowding, and ...

journal_title：Molecular and cellular biology

authors： Fite K,Elkhadragy L,Gomez-Cambronero J

更新日期：2016-01-19 00:00:00

abstract：:Very little is known about the mechanisms mediating longevities of mRNAs. As a means of identifying potential cis- and trans-acting elements which stabilize an individual mRNA, naturally occurring mutations that decreased stability of the normally long-lived globin mRNA were analyzed. Our previous studies demonstrated...

journal_title：Molecular and cellular biology

authors： Weiss IM,Liebhaber SA

更新日期：1995-05-01 00:00:00

abstract：:The upstream promoter sequences of the human carbonic anhydrase II (CA II) gene have been studied by 5' deletion analysis. Promoter activity was assayed by transfection and chloramphenicol acetyltransferase assay in both human HeLa cells and murine L cells. This investigation showed that the CA II promoter is comparab...

journal_title：Molecular and cellular biology

authors： Shapiro LH,Venta PJ,Tashian RE

更新日期：1987-12-01 00:00:00

abstract：:Double-stranded RNA (dsRNA) accumulates in virus-infected mammalian cells and signals the activation of host defense pathways of the interferon system. We describe here a novel form of dsRNA-triggered signaling that leads to the stimulation of the p38 mitogen-activated protein kinase (p38 MAPK) and the c-Jun NH(2)-ter...

journal_title：Molecular and cellular biology

authors： Iordanov MS,Paranjape JM,Zhou A,Wong J,Williams BR,Meurs EF,Silverman RH,Magun BE

更新日期：2000-01-01 00:00:00

abstract：:Entry into mitosis is controlled by the cyclin-dependent kinase CDK1 and can be delayed in response to DNA damage. In some systems, such G(2)/M arrest has been shown to reflect the stabilization of inhibitory phosphorylation sites on CDK1. In human cells, full G(2) arrest appears to involve additional mechanisms. We d...

journal_title：Molecular and cellular biology

authors： Badie C,Itzhaki JE,Sullivan MJ,Carpenter AJ,Porter AC

更新日期：2000-04-01 00:00:00

abstract：:The chicken immunoglobulin light-chain gene (IgL) encodes only a single variable gene segment capable of recombination. To generate an immune repertoire, chickens diversify this unique rearranged VL gene segment during B-cell development in the bursa of Fabricius. Sequence analysis of IgL cDNAs suggests that both gene...

journal_title：Molecular and cellular biology

authors： Kim S,Humphries EH,Tjoelker L,Carlson L,Thompson CB

更新日期：1990-06-01 00:00:00

abstract：:We have cloned CDC9, the structural gene for Saccharomyces cerevisiae DNA ligase, and investigated its transcriptional regulation both as a function of cell cycle stage and after UV irradiation. The steady-state level of DNA ligase mRNA increases at least fourfold in late G1, after the completion of start but before S...

journal_title：Molecular and cellular biology

authors： Peterson TA,Prakash L,Prakash S,Osley MA,Reed SI

更新日期：1985-01-01 00:00:00

abstract：:We have investigated the relationship between Xenopus laevis c-mos (mosXe) and the cyclin B component of maturation-promoting factor. Microinjection of Xenopus oocytes with in vitro-synthesized RNAs encoding Xenopus cyclin B1 or cyclin B2 induces the progression of meiosis, characterized by germinal vesicle breakdown ...

journal_title：Molecular and cellular biology

authors： Freeman RS,Ballantyne SM,Donoghue DJ

更新日期：1991-03-01 00:00:00

abstract：:We have previously shown by affinity chromatography that RAP30 and RAP74 are the mammalian proteins that have the highest affinity for RNA polymerase II. Here we show that RAP30 binds to RAP74 and that the RAP30-RAP74 complex (RAP30/74) is required for accurate initiation by RNA polymerase II. RAP30/74 is required for...

journal_title：Molecular and cellular biology

authors： Burton ZF,Killeen M,Sopta M,Ortolan LG,Greenblatt J

更新日期：1988-04-01 00:00:00

abstract：:The Saccharomyces cerevisiae Srs2 protein is involved in DNA repair and recombination. In order to gain better insight into the roles of Srs2, we performed a screen to identify mutations that are synthetically lethal with an srs2 deletion. One of them is a mutated allele of the ULP1 gene that encodes a protease specif...

journal_title：Molecular and cellular biology

authors： Soustelle C,Vernis L,Fréon K,Reynaud-Angelin A,Chanet R,Fabre F,Heude M

更新日期：2004-06-01 00:00:00

abstract：:The Suppressor of the Hairy wing [Su(Hw)] binding region within the gypsy retrotransposon is the best known chromatin insulator in Drosophila melanogaster. According to previous data, two copies of the gypsy insulator inserted between an enhancer and a promoter neutralize each other's actions, which is indicative of a...

journal_title：Molecular and cellular biology

authors： Kravchenko E,Savitskaya E,Kravchuk O,Parshikov A,Georgiev P,Savitsky M

更新日期：2005-11-01 00:00:00