Abstract:
:GATA-1, a transcription factor essential for the development of the erythroid lineage, contains two adjacent highly conserved zinc finger motifs. The carboxy-terminal finger is necessary and sufficient for specific binding to the consensus GATA recognition sequence: mutant proteins containing only the amino-terminal finger do not bind. Here we identify a DNA sequence (GATApal) for which the GATA-1 amino-terminal finger makes a critical contribution to the strength of binding. The site occurs in the GATA-1 gene promoters of chickens, mice, and humans but occurs very infrequently in other vertebrate genes known to be regulated by GATA proteins. GATApal is a palindromic site composed of one complete [(A/T)GATA(A/G)] and one partial (GAT) canonical motif. Deletion of the partial motif changes the site to a normal GATA site and also reduces by as much as eightfold the activity of the GATA-1 promoter in an erythroid precursor cell. We propose that GATApal is important for positive regulation of GATA-1 expression in erythroid cells.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Trainor CD,Omichinski JG,Vandergon TL,Gronenborn AM,Clore GM,Felsenfeld Gdoi
10.1128/mcb.16.5.2238subject
Has Abstractpub_date
1996-05-01 00:00:00pages
2238-47issue
5eissn
0270-7306issn
1098-5549journal_volume
16pub_type
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