Rearrangement of rat immunoglobulin E heavy-chain and c-myc genes in the B-cell immunocytoma IR162.

abstract：:To interrogate genes essential for cell growth, proliferation and survival in human cells, we carried out a genome-wide clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 screen in a B-cell lymphoma line using a custom extended-knockout (EKO) library of 278,754 single-guide RNAs (sgRNAs) that targe...

journal_title：Molecular and cellular biology

authors： Bertomeu T,Coulombe-Huntington J,Chatr-Aryamontri A,Bourdages KG,Coyaud E,Raught B,Xia Y,Tyers M

更新日期：2017-12-13 00:00:00

abstract：:Akt is activated on the plasma membrane and its substrates are distributed throughout various cellular compartments. To phosphorylate its substrates, Akt needs to be recruited to specific intracellular compartments. Thus, regulation of Akt cellular compartmentalization constitutes an important mechanism to specify Akt...

journal_title：Molecular and cellular biology

authors： Ding J,Du K

更新日期：2009-03-01 00:00:00

abstract：:The Tor pathway mediates cell growth in response to nutrient availability, in part by inducing ribosomal protein (RP) gene expression via an unknown mechanism. Expression of RP genes coincides with recruitment of the Esa1 histone acetylase to RP gene promoters. We show that inhibition of Tor with rapamycin releases Es...

journal_title：Molecular and cellular biology

authors： Rohde JR,Cardenas ME

更新日期：2003-01-01 00:00:00

abstract：:We have studied leucine transport in several Chinese hamster-human hybrid cell lines obtained by fusion of a temperature-sensitive line of Chinese hamster ovary cells, ts025C1, and normal human leukocytes. A hybrid cell line exhibiting a twofold increase in L-leucine uptake over that in the parental cell line was foun...

journal_title：Molecular and cellular biology

authors： Lobaton CD,Moreno A,Oxender DL

更新日期：1984-03-01 00:00:00

abstract：:The first processing step in the maturation of mouse precursor rRNA involves cleavage at nucleotide ca. +650, at the 5' border of a 200-nucleotide region that is conserved across mammals and contains the sequences that direct the processing. To identify the relevant sequence elements, we used rRNAs with small internal...

journal_title：Molecular and cellular biology

authors： Craig N,Kass S,Sollner-Webb B

更新日期：1991-01-01 00:00:00

abstract：:We have previously described a 160-bp enhancer (BCE-1) in the bovine beta-casein gene that is activated in the presence of prolactin and extracellular matrix (ECM). Here we report the characterization of the enhancer by deletion and site-directed mutagenesis, electrophoretic mobility shift analysis, and in vivo footpr...

journal_title：Molecular and cellular biology

authors： Myers CA,Schmidhauser C,Mellentin-Michelotti J,Fragoso G,Roskelley CD,Casperson G,Mossi R,Pujuguet P,Hager G,Bissell MJ

更新日期：1998-04-01 00:00:00

abstract：:In Saccharomyces cerevisiae, many amino acid biosynthetic pathways are coregulated by a complex general control system: starvation for a single amino acid results in the derepression of amino acid biosynthetic genes in multiple pathways. Derepression of these genes is mediated by positive (GCN) and negative (GCD) regu...

journal_title：Molecular and cellular biology

authors： Myers PL,Skvirsky RC,Greenberg ML,Greer H

更新日期：1986-09-01 00:00:00

abstract：:The active 17S U2 small nuclear ribonucleoprotein particle (snRNP), which binds to the intron branch site during the formation of the prespliceosome, is assembled in vitro by sequential interactions of the essential splicing factors SF3b and SF3a with the 12S U2 snRNP. We have analyzed the function of individual subun...

journal_title：Molecular and cellular biology

authors： Nesic D,Krämer A

更新日期：2001-10-01 00:00:00

abstract：:The structural maintenance of chromosomes (Smc) family members Smc5 and Smc6 are both essential in budding and fission yeasts. Yeast smc5/6 mutants are hypersensitive to DNA damage, and Smc5/6 is recruited to HO-induced double-strand breaks (DSBs), facilitating intersister chromatid recombinational repair. To determin...

journal_title：Molecular and cellular biology

authors： Stephan AK,Kliszczak M,Dodson H,Cooley C,Morrison CG

更新日期：2011-04-01 00:00:00

abstract：:The E2A-HLF fusion gene, formed by the t(17;19)(q22;p13) translocation in childhood acute pro-B-cell leukemia, encodes a hybrid protein that contains the paired trans-activation domains of E2A (E12/E47) linked to the basic region/leucine zipper DNA-binding and dimerization domain of hepatic leukemia factor (HLF). To a...

journal_title：Molecular and cellular biology

authors： Yoshihara T,Inaba T,Shapiro LH,Kato JY,Look AT

更新日期：1995-06-01 00:00:00

abstract：:The C-terminal domain of the RNA polymerase II largest subunit (the Rpb1 CTD) is composed of tandem heptad repeats of the consensus sequence Y(1)S(2)P(3)T(4)S(5)P(6)S(7). We reported previously that Thr 4 is phosphorylated and functions in histone mRNA 3'-end formation in chicken DT40 cells. Here, we have extended our...

journal_title：Molecular and cellular biology

authors： Hsin JP,Xiang K,Manley JL

更新日期：2014-07-01 00:00:00

abstract：:The gene encoding the SNF5/Ini1 core subunit of the SWI/SNF chromatin remodeling complex is a tumor suppressor in humans and mice, with an essential role in early embryonic development. To investigate further the function of this gene, we have generated a Cre/lox-conditional mouse line. We demonstrate that Snf5 deleti...

journal_title：Molecular and cellular biology

authors： Klochendler-Yeivin A,Picarsky E,Yaniv M

更新日期：2006-04-01 00:00:00

abstract：:Methyl-accepting assays and a sensitive method for labeling specific CpG sites have been used to show that the DNA of F9 embryonal carcinoma cells decreases in 5-methylcytosine content by ca. 9% during retinoic acid-induced differentiation, whereas the DNA of dimethyl sulfoxide-induced Friend murine erythroleukemia (M...

journal_title：Molecular and cellular biology

authors： Bestor TH,Hellewell SB,Ingram VM

更新日期：1984-09-01 00:00:00

abstract：:Osteoclast differentiation factor (ODF)/receptor activator of NF-kappaB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the CCAAT box on the ODF promoter and regulates its basal transcriptional activity. The CCAAT box on the ODF gene is required...

journal_title：Molecular and cellular biology

authors： Kabe Y,Yamada J,Uga H,Yamaguchi Y,Wada T,Handa H

更新日期：2005-01-01 00:00:00

abstract：:In neural development, Notch signaling plays a key role in restricting neuronal differentiation, promoting the maintenance of progenitor cells. Classically, Notch signaling causes transactivation of Hairy-enhancer of Split (HES) genes which leads to transcriptional repression of neural determination and differentiatio...

journal_title：Molecular and cellular biology

authors： Sriuranpong V,Borges MW,Strock CL,Nakakura EK,Watkins DN,Blaumueller CM,Nelkin BD,Ball DW

更新日期：2002-05-01 00:00:00

abstract：:The lck proto-oncogene encodes a lymphocyte-specific member of the src family of protein tyrosine kinases. Here we demonstrate that pp56lck is phosphorylated in vivo at a carboxy-terminal tyrosine residue (Tyr-505) analogous to Tyr-527 of pp60c-src. Substitution of phenylalanine for tyrosine at this position resulted ...

journal_title：Molecular and cellular biology

authors： Marth JD,Cooper JA,King CS,Ziegler SF,Tinker DA,Overell RW,Krebs EG,Perlmutter RM

更新日期：1988-02-01 00:00:00

abstract：:Expression of vascular endothelial growth factor (VEGF) increases in cancer cells during hypoxia. Herein, we report that the MDM2 oncoprotein plays a role in hypoxia-mediated VEGF upregulation. In studying the characteristics of MDM2 and VEGF expression in neuroblastoma cells, we found that hypoxia induced significant...

journal_title：Molecular and cellular biology

authors： Zhou S,Gu L,He J,Zhang H,Zhou M

更新日期：2011-12-01 00:00:00

abstract：:In CBFbeta-SMMHC, core binding factor beta (CBFbeta) is fused to the alpha-helical rod domain of smooth muscle myosin heavy chain (SMMHC). We generated Ba/F3 hematopoietic cells expressing a CBFbeta-SMMHC variant lacking 28 amino acids homologous to the assembly competence domain (ACD) required for multimerization of ...

journal_title：Molecular and cellular biology

authors： Kummalue T,Lou J,Friedman AD

更新日期：2002-12-01 00:00:00

abstract：:Spatial control of cytokinesis is essential for proper cell division. The molecular mechanisms that anchor the dynamic assembly and constriction of the cytokinetic ring at the plasma membrane remain unclear. In the fission yeast Schizosaccharomyces pombe, the cytokinetic ring is assembled in the cell middle from corti...

journal_title：Molecular and cellular biology

authors： Guzman-Vendrell M,Baldissard S,Almonacid M,Mayeux A,Paoletti A,Moseley JB

更新日期：2013-01-01 00:00:00

abstract：:CHIP is a cochaperone of Hsp70 that inhibits Hsp70-dependent refolding in vitro. However, the effect of altered expression of CHIP on the fate of unfolded proteins in mammalian cells has not been determined. Surprisingly, we found that overexpression of CHIP in fibroblasts increased the refolding of proteins after the...

journal_title：Molecular and cellular biology

authors： Kampinga HH,Kanon B,Salomons FA,Kabakov AE,Patterson C

更新日期：2003-07-01 00:00:00

abstract：:A fragment of the human gene for c-erb-B was used to map homologous sequences in mice. Analysis of somatic cell hybrids and recombinant inbred and congenic mouse strains indicated that this gene, designated Erbb, is closely linked to the gene for alpha-globin on mouse chromosome 11. Several genes controlling hematopoi...

journal_title：Molecular and cellular biology

authors： Silver J,Whitney JB 3rd,Kozak C,Hollis G,Kirsch I

更新日期：1985-07-01 00:00:00

abstract：:The use of small-molecule inhibitors to study molecular components of cellular signal transduction pathways provides a means of analysis complementary to currently used techniques, such as antisense, dominant-negative (interfering) mutants and constitutively activated mutants. We have identified and characterized a sm...

journal_title：Molecular and cellular biology

authors： Blake RA,Broome MA,Liu X,Wu J,Gishizky M,Sun L,Courtneidge SA

更新日期：2000-12-01 00:00:00

abstract：:We report in vivo analysis of histone and RNA polymerase II (pol II) occupancy at the 601 element, which functions as a strong in vitro nucleosome-positioning element and transcriptional pause site. Surprisingly, nucleosomes were not strongly positioned over the 601 element inserted either within a yeast chromosomal o...

journal_title：Molecular and cellular biology

authors： Perales R,Zhang L,Bentley D

更新日期：2011-08-01 00:00:00

abstract：:RAG1 and RAG2 catalyze the first DNA cleavage steps in V(D)J recombination. We demonstrate that the isolated central domain of RAG1 has inherent single-stranded (ss) DNA cleavage activity, which does not require, but is enhanced by, RAG2. The central domain, therefore, contains the active-site residues necessary to pe...

journal_title：Molecular and cellular biology

authors： De P,Peak MM,Rodgers KK

更新日期：2004-08-01 00:00:00

abstract：:During mammalian vascular development, endothelial cells form a complex array of vessels that differ markedly in structure and function, but the molecular basis for this vascular complexity is poorly understood. Recent insights into endothelial diversity have come from the identification of molecular markers expressed...

journal_title：Molecular and cellular biology

authors： Xu B,deWaal RM,Mor-Vaknin N,Hibbard C,Markovitz DM,Kahn ML

更新日期：2004-10-01 00:00:00

abstract：:The four receptor tyrosine kinases of the ErbB family play essential roles in several physiological processes and have also been implicated in tumor generation and/or progression. Activation of ErbB1/EGFR is mainly triggered by epidermal growth factor (EGF) and other related ligands, while activation of ErbB2, ErbB3, ...

journal_title：Molecular and cellular biology

authors： Esparís-Ogando A,Díaz-Rodríguez E,Montero JC,Yuste L,Crespo P,Pandiella A

更新日期：2002-01-01 00:00:00

abstract：:Ubiquitously expressed transcription factors play an integral role in establishing and regulating patterns of gene transcription. Common factor 1 (CF1) is a ubiquitously expressed DNA-binding protein previously identified in our laboratory. We show here that CF1 recognizes sites in several diverse transcription elemen...

journal_title：Molecular and cellular biology

authors： Riggs KJ,Merrell KT,Wilson G,Calame K

更新日期：1991-03-01 00:00:00

abstract：:Centrosomes ensure accurate chromosome segregation by directing spindle bipolarity. Loss of centrosome regulation results in centrosome amplification, multipolar mitosis and aneuploidy. Since centrosome amplification is common in premalignant lesions and breast tumors, it is proposed to play a central role in breast t...

journal_title：Molecular and cellular biology

authors： Lee MY,Moreno CS,Saavedra HI

更新日期：2014-07-01 00:00:00

abstract：:Previous studies have shown that exposure of cells to high levels of replicational stress leads to permanent proliferation arrest that does not require p53. We have examined cellular responses to therapeutically relevant low levels of replicational stress that allow limited proliferation. Chronic exposure to low conce...

journal_title：Molecular and cellular biology

authors： Marusyk A,Wheeler LJ,Mathews CK,DeGregori J

更新日期：2007-08-01 00:00:00

abstract：:In the budding yeast Saccharomyces cerevisiae, mutations in the essential gene CDC1 cause defects in Golgi inheritance and actin polarization. However, the biochemical function of Cdc1p is unknown. Previous work showed that cdc1 mutants accumulate intracellular Ca(2+) and display enhanced sensitivity to the extracellu...

journal_title：Molecular and cellular biology

authors： Losev E,Papanikou E,Rossanese OW,Glick BS

更新日期：2008-05-01 00:00:00