Linear distance from the locus control region determines epsilon-globin transcriptional activity.

abstract：:Phospholipase C-gamma2 (PLC-gamma2) plays an important role in B-cell signaling. Phosphorylation of various tyrosine residues of PLC-gamma2 has been implicated in regulation of its lipase activity. With the use of antibodies specific for each of the putative phosphorylation sites, we have now shown that PLC-gamma2 is ...

journal_title：Molecular and cellular biology

authors： Kim YJ,Sekiya F,Poulin B,Bae YS,Rhee SG

更新日期：2004-11-01 00:00:00

abstract：:Poly(A)-binding protein (PABP) stimulates translation initiation by binding simultaneously to the mRNA poly(A) tail and eukaryotic translation initiation factor 4G (eIF4G). PABP activity is regulated by PABP-interacting (Paip) proteins. Paip1 binds PABP and stimulates translation by an unknown mechanism. Here, we desc...

journal_title：Molecular and cellular biology

authors： Martineau Y,Derry MC,Wang X,Yanagiya A,Berlanga JJ,Shyu AB,Imataka H,Gehring K,Sonenberg N

更新日期：2008-11-01 00:00:00

abstract：:To examine the sequence complexity and differential expression of human alpha-tubulin genes, we constructed cDNA libraries from two unrelated tissue types (epidermis and fetal brain). The complete sequence of a positively hybridizing alpha-tubulin clone from each library is described. Each is shown to represent an abu...

journal_title：Molecular and cellular biology

authors： Cowan NJ,Dobner PR,Fuchs EV,Cleveland DW

更新日期：1983-10-01 00:00:00

abstract：:The simian virus 40 72-base-pair repeats substituted for the polyomavirus enhancer, allowing replication and transcription in mouse 3T6 but not monkey CV-1 cells. A polyomavirus genome containing the entire simian virus 40 control region replicated at low levels in 3T6 and CV-1 cells; however, transcripts were detecte...

journal_title：Molecular and cellular biology

authors： Campbell BA,Villarreal LP

更新日期：1985-06-01 00:00:00

abstract：:MOT1 is an essential Saccharomyces cerevisiae protein and a member of the SNF2/SWI2 family of ATPases. MOT1 functions by removing TATA-binding protein (TBP) from DNA, and as a consequence, MOT1 can regulate transcription both in vitro and in vivo. Here we describe the in vivo and in vitro activities of MOT1 deletion a...

journal_title：Molecular and cellular biology

authors： Auble DT,Wang D,Post KW,Hahn S

更新日期：1997-08-01 00:00:00

abstract：:Cytoplasmic poly(A) elongation is one mechanism that regulates translational recruitment of maternal mRNA in early development. In Xenopus laevis, poly(A) elongation is controlled by two cis elements in the 3' untranslated regions of responsive mRNAs: the hexanucleotide AAUAAA and a U-rich structure with the general s...

journal_title：Molecular and cellular biology

authors： Stebbins-Boaz B,Richter JD

更新日期：1994-09-01 00:00:00

abstract：:To determine whether regulation of c-myc proteins occurs during the differentiation of murine erythroleukemia cells, we examined c-myc protein synthesis and accumulation throughout dimethyl sulfoxide (DMSO)- or hypoxanthine-induced differentiation. c-myc protein expression exhibited an overall biphasic reduction, with...

journal_title：Molecular and cellular biology

authors： Spotts GD,Hann SR

更新日期：1990-08-01 00:00:00

abstract：:mRNA deadenylation is a key process in the regulation of translation and mRNA turnover. In Saccharomyces cerevisiae, deadenylation is primarily carried out by the Ccr4p and Caf1p/Pop2p subunits of the Ccr4-Not complex, which is conserved in eukaryotes including humans. Here we have identified an unconventional human C...

journal_title：Molecular and cellular biology

authors： Wagner E,Clement SL,Lykke-Andersen J

更新日期：2007-03-01 00:00:00

abstract：:RNase H1 in mammalian cells is present in nuclei and mitochondria. Its absence in mitochondria results in embryonic lethality due to the failure to amplify mitochondrial DNA (mtDNA). Dual localization to mitochondria and nuclei results from differential translation initiation at two in-frame AUGs (M1 and M27) of a sin...

journal_title：Molecular and cellular biology

authors： Suzuki Y,Holmes JB,Cerritelli SM,Sakhuja K,Minczuk M,Holt IJ,Crouch RJ

更新日期：2010-11-01 00:00:00

abstract：:The abundance of crystallins (> 80% of the soluble protein) in the ocular lens provides advantageous markers for selective gene expression during cellular differentiation. Here we show by functional and protein-DNA binding experiments that the chicken alpha A-crystallin gene is regulated by at least five control eleme...

journal_title：Molecular and cellular biology

authors： Cvekl A,Sax CM,Bresnick EH,Piatigorsky J

更新日期：1994-11-01 00:00:00

abstract：:Genetic and biochemical evidence suggests that the Ras protooncogene product regulates the activation of the Raf kinase pathway, leading to the proposal that Raf is a direct mitogenic effector of activated Ras. Here we report the use of a novel competition assay to measure in vitro the relative affinity of the c-Raf-1...

journal_title：Molecular and cellular biology

authors： Chuang E,Barnard D,Hettich L,Zhang XF,Avruch J,Marshall MS

更新日期：1994-08-01 00:00:00

abstract：:DNA-binding studies with Saccharomyces cerevisiae TFIID point mutants indicated that TFIIA interacts with the basic repeat region of TFIID and induces structural changes. The latter was shown by the ability of TFIIA to compensate for TFIID point mutants defective for DNA binding. Interaction with TFIIA also rendered T...

journal_title：Molecular and cellular biology

authors： Lee DK,DeJong J,Hashimoto S,Horikoshi M,Roeder RG

更新日期：1992-11-01 00:00:00

abstract：:Hybridization-complementation studies indicated that mutations in multiple genes can render Chinese hamster cells resistant to the alkaloid translation inhibitor emetine. Two of the genes, emtA and emtB, recognized in Chinese hamster lung and ovary cell lines, respectively, are known to affect the ribosomes of the cel...

journal_title：Molecular and cellular biology

authors： Nielsen-Smith K,McGill S,Frahm M,Roufa DJ

更新日期：1983-02-01 00:00:00

abstract：:Malignant gliomas are highly lethal neoplasms with limited treatment options. We previously found that the heparan sulfate proteoglycan glypican 1 (GPC1) is universally and highly expressed in human gliomas. In this study, we investigated the biological activity of GPC1 expression in both human glioma cells and normal...

journal_title：Molecular and cellular biology

authors： Qiao D,Meyer K,Friedl A

更新日期：2013-11-01 00:00:00

abstract：:T-lymphocyte stimulation requires activation of several protein kinases, including the major phorbol ester receptor protein kinase C (PKC), ultimately leading to induction of lymphokines, such as interleukin-2 (IL-2). The revelant PKC isoforms which are involved in the activation cascades of nuclear transcription fact...

journal_title：Molecular and cellular biology

authors： Baier-Bitterlich G,Uberall F,Bauer B,Fresser F,Wachter H,Grunicke H,Utermann G,Altman A,Baier G

更新日期：1996-04-01 00:00:00

abstract：:Previous analyses defined a proliferating cell nuclear antigen (PCNA) E1A-responsive element (PERE) in the PCNA promoter that is essential for transactivation by the 243-residue product of the adenovirus type 2 E1A 12S mRNA (E1A 243R). In this report, we show that the PERE activates a heterologous basal promoter and c...

journal_title：Molecular and cellular biology

authors： Morris GF,Labrie C,Mathews MB

更新日期：1994-01-01 00:00:00

abstract：:The cellular mechanisms involved in chronic inflammatory processes are poorly understood. This is especially true for the role of macrophages, which figure prominently in the inflammatory response. Two proteins, MRP8 and MRP14, which are expressed in infiltrate macrophages during inflammatory reactions but not in norm...

journal_title：Molecular and cellular biology

authors： Lagasse E,Clerc RG

更新日期：1988-06-01 00:00:00

abstract：:Insults to cellular health cause p53 protein accumulation, and loss of p53 function leads to tumorigenesis. Thus, p53 has to be tightly controlled. Here we report that the BTB/POZ domain transcription factor PATZ1 (MAZR), previously known for its transcriptional suppressor functions in T lymphocytes, is a crucial regu...

journal_title：Molecular and cellular biology

authors： Keskin N,Deniz E,Eryilmaz J,Un M,Batur T,Ersahin T,Cetin Atalay R,Sakaguchi S,Ellmeier W,Erman B

更新日期：2015-05-01 00:00:00

abstract：:During mammalian vascular development, endothelial cells form a complex array of vessels that differ markedly in structure and function, but the molecular basis for this vascular complexity is poorly understood. Recent insights into endothelial diversity have come from the identification of molecular markers expressed...

journal_title：Molecular and cellular biology

authors： Xu B,deWaal RM,Mor-Vaknin N,Hibbard C,Markovitz DM,Kahn ML

更新日期：2004-10-01 00:00:00

abstract：:Csn3 (Cops3) maps to the mouse chromosome 11 region syntenic to the common deletion interval for the Smith-Magenis syndrome, a contiguous gene deletion syndrome. It encodes the third subunit of an eight-subunit protein complex, the COP9 signalosome (CSN), which controls a wide variety of molecules of different functio...

journal_title：Molecular and cellular biology

authors： Yan J,Walz K,Nakamura H,Carattini-Rivera S,Zhao Q,Vogel H,Wei N,Justice MJ,Bradley A,Lupski JR

更新日期：2003-10-01 00:00:00

abstract：:TRIF is an essential adaptor for Toll-like receptor 3/4 (TLR3/4) signaling to activate transcription factor interferon regulatory factor 3 (IRF-3). We examined the molecular mechanism of TLR3 signaling and found that TLR3 stimulation by double-stranded RNA (dsRNA) induces phosphorylation of TRIF at Ser210 and is requi...

journal_title：Molecular and cellular biology

authors： Abe H,Satoh J,Shirasaka Y,Kogure A,Kato H,Ito S,Fujita T

更新日期：2020-02-12 00:00:00

abstract：:Using competition mobility shift, methylation interference, and proteolytic clipping DNA binding assays, we demonstrate that the protein binding the major histocompatibility complex A beta CCAAT box is indistinguishable from the protein previously named NF-Y, which binds the major histocompatibility complex E alpha CC...

journal_title：Molecular and cellular biology

authors： Kern MJ,Woodward JG

更新日期：1991-01-01 00:00:00

abstract：:Quantitative immunoelectron microscopy and subcellular fractionation established the site of endoplasmic reticulum (ER)-Golgi transport arrest induced by the phosphatase inhibitor okadaic acid (OA). OA induced the disappearance of transitional element tubules and accumulation of the anterograde-transported Chandipura ...

journal_title：Molecular and cellular biology

authors： Pryde JG,Farmaki T,Lucocq JM

更新日期：1998-02-01 00:00:00

abstract：:sra5 mutations in Saccharomyces cerevisiae were previously shown to suppress the inefficient growth of ras2 strains on nonfermentable carbon sources and to result in deficient low-Km cyclic AMP (cAMP) phosphodiesterase activity. We have cloned SRA5 by complementation. It maps to the right arm of chromosome XV, tightly...

journal_title：Molecular and cellular biology

authors： Wilson RB,Tatchell K

更新日期：1988-01-01 00:00:00

abstract：:We have examined the ability of the simian virus 40 72-base pair enhancer segment to simultaneously activate multiple transcription units with plasmids that contain one, two, or three simian virus 40-based transcription units in various arrangements. After transfection into CV1 cells, the expression of a marker gene, ...

journal_title：Molecular and cellular biology

authors： Kadesch T,Berg P

更新日期：1986-07-01 00:00:00

abstract：:The role of the target cell in its own death mediated by cytotoxic T lymphocytes (CTL) has been controversial. The ability of the pore-forming granule components of CTL to induce target cell death directly has been taken to suggest an essentially passive role for the target. This view of CTL-mediated killing ascribes ...

journal_title：Molecular and cellular biology

authors： Ucker DS,Wilson JD,Hebshi LD

更新日期：1994-01-01 00:00:00

abstract：:Calcineurin, or PP2B, plays a critical role in mediating Ca2+-dependent signaling in many cell types. In yeast cells, this highly conserved protein phosphatase regulates aspects of ion homeostasis and cell wall synthesis. We show that calcineurin mutants are sensitive to high concentrations of Mn2+ and identify two ge...

journal_title：Molecular and cellular biology

authors： Pozos TC,Sekler I,Cyert MS

更新日期：1996-07-01 00:00:00

abstract：:Ribosomes can be programmed to shift from one reading frame to another during translation. Hepatitis C virus (HCV) uses such a mechanism to produce F protein from the -2/+1 reading frame. We now report that the HCV frameshift signal can mediate the synthesis of the core protein of the zero frame, the F protein of the ...

journal_title：Molecular and cellular biology

authors： Choi J,Xu Z,Ou JH

更新日期：2003-03-01 00:00:00

abstract：:It has been hypothesized that protein factors may protect CpG islands from methyltransferase during development and that demethylation may involve protein-DNA interactions at demethylated sites. However, direct evidence has been lacking. In this study, demethylation at the EBNA-1 binding sites of the Epstein-Barr viru...

journal_title：Molecular and cellular biology

authors： Hsieh CL

更新日期：1999-01-01 00:00:00

abstract：:HMGN proteins promote chromatin unfolding, enhance access to nucleosomes, and modulate transcription from chromatin templates. It is not known whether they act indiscriminately as general modulators of transcription or whether they regulate specific gene expression. Here, we investigated the role of HMGN3, a recently ...

journal_title：Molecular and cellular biology

authors： West KL,Castellini MA,Duncan MK,Bustin M

更新日期：2004-05-01 00:00:00