Abstract:
:The cellular mechanisms involved in chronic inflammatory processes are poorly understood. This is especially true for the role of macrophages, which figure prominently in the inflammatory response. Two proteins, MRP8 and MRP14, which are expressed in infiltrate macrophages during inflammatory reactions but not in normal tissue macrophages, have been characterized. Here we report that MRP8 and MRP14 mRNAs are specifically expressed in human cells of myeloid origin and that their expression is regulated during monocyte-macrophage and granulocyte differentiation. To initiate the analysis of cis-acting elements governing the tissue-specific expression of the MRP genes, we cloned the human genes encoding MRP8 and MRP14. Both genes contain three exons, are single copy, and have a strikingly similar organization. They belong to a novel subfamily of highly homologous calcium-binding proteins which includes S100 alpha, S100 beta, intestinal calcium-binding protein, P11, and calcyclin (2A9). A transient expression assay was devised to investigate the tissue-specific regulatory elements responsible for MRP gene expression after differentiation in leukemia HL60 cells. The results of this investigation demonstrated that the cis-acting elements responsible for MRP expression are present on the cloned DNA fragment containing the MRP gene loci.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Lagasse E,Clerc RGdoi
10.1128/mcb.8.6.2402subject
Has Abstractpub_date
1988-06-01 00:00:00pages
2402-10issue
6eissn
0270-7306issn
1098-5549journal_volume
8pub_type
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.19.6.4200
更新日期:1999-06-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.7.1.237
更新日期:1987-01-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.9.9.4113
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.22.8.2556-2563.2002
更新日期:2002-04-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01636-09
更新日期:2010-10-01 00:00:00
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journal_title:Molecular and cellular biology
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更新日期:1997-04-01 00:00:00
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journal_title:Molecular and cellular biology
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更新日期:2005-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-05-01 00:00:00