Transcription from the polyoma late promoter in cells stably transformed by chimeric plasmids.


:We have examined the expression of chimeric plasmids containing coding sequences for the herpes simplex virus thymidine kinase (tk) gene or the Tn5 gene for neomycin resistance (neo) linked to the late promoter of polyoma DNA. Although polyoma late genes are generally not expressed in transformed cells containing only integrated viral DNA molecules, rat tk- or wild-type cells transfected with the tk- or neo-containing plasmids were capable of growing in medium containing either hypoxanthine-aminopterin-thymidine or G418, respectively, under conditions nonpermissive for extrachromosomal DNA replication, indicating that the tk or neo genes were fully expressed. Moreover, cells were capable of growth in either hypoxanthine-aminopterin-thymidine or G418, even in the absence of direct selection for this activity. Northern analysis indicated steady-state levels of tk or neo transcripts that approximated the levels of polyoma early transcripts. S1 analysis showed that these transcripts initiated within the late promoter of polyoma and that their 5' ends mapped at positions similar or identical to those utilized during late lytic infection. The effect of substitution of polyadenylation signals was examined. Although plasmids containing the polyoma early polyadenylation signal were more efficient in conferring to cells a stable G418-resistant phenotype than similar constructions using the late signal, both signals were found to be effectively utilized. This indicates that the inability to detect late transcripts in polyoma-transformed cells in the absence of free viral DNA production is not an effect of inefficient mRNA cleavage or polyadenylation. Our results suggest that late gene expression in integrated polyoma genomes is not regulated at the level of message initiation but, most likely, through posttranscriptional events.


Mol Cell Biol


Kern FG,Basilico C




Has Abstract


1985-04-01 00:00:00












  • Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequences.

    abstract::The Saccharomyces cerevisiae PGK (phosphoglycerate kinase) gene encodes one of the most abundant mRNA and protein species in the cell. To identify the promoter sequences required for the efficient expression of PGK, we undertook a detailed internal deletion analysis of the 5' noncoding region of the gene. Our analysis...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ogden JE,Stanway C,Kim S,Mellor J,Kingsman AJ,Kingsman SM

    更新日期:1986-12-01 00:00:00

  • A mitochondrial retroplasmid integrates into mitochondrial DNA by a novel mechanism involving the synthesis of a hybrid cDNA and homologous recombination.

    abstract::The Mauriceville and Varkud mitochondrial plasmids of Neurospora spp. are closely related, small circular DNAs that propagate via an RNA intermediate and reverse transcription. Although the plasmids ordinarily replicate autonomously, they can also integrate into mitochondrial DNA (mtDNA), yielding defective mtDNAs tha...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Chiang CC,Kennell JC,Wanner LA,Lambowitz AM

    更新日期:1994-10-01 00:00:00

  • Kinds and spectrum of mutations induced by 1-nitrosopyrene adducts during plasmid replication in human cells.

    abstract::1-Nitropyrene has been shown in bacterial assays to be the principal mutagenic agent in diesel emission particulates. It has also been shown to be mutagenic in human fibroblasts and carcinogenic in animals. To investigate the kinds of mutations induced by this carcinogen and compare them with those induced by a struct...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Yang JL,Maher VM,McCormick JJ

    更新日期:1988-08-01 00:00:00

  • Novel splicing factor RBM25 modulates Bcl-x pre-mRNA 5' splice site selection.

    abstract::RBM25 has been shown to associate with splicing cofactors SRm160/300 and assembled splicing complexes, but little is known about its splicing regulation. Here, we characterize the functional role of RBM25 in alternative pre-mRNA splicing. Increased RBM25 expression correlated with increased apoptosis and specifically ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zhou A,Ou AC,Cho A,Benz EJ Jr,Huang SC

    更新日期:2008-10-01 00:00:00

  • Alternative processing of RNA transcribed from NMYC.

    abstract::NMYC is a gene whose amplification and overexpression have been implicated in the generation of certain human malignancies. Little is known of how the expression of NMYC is normally controlled. We have therefore characterized transcription from the gene and the structure and stability of the resulting mRNAs. Transcrip...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Stanton LW,Bishop JM

    更新日期:1987-12-01 00:00:00

  • Recognition DNA sequences of interferon regulatory factor 1 (IRF-1) and IRF-2, regulators of cell growth and the interferon system.

    abstract::Interferon (IFN) regulatory factor 1 (IRF-1) and IRF-2 were originally identified as transcription factors involved in the regulation of the IFN system. IRF-1 functions as a transcriptional activator, while IRF-2 represses IRF-1 function. More recently, evidence has been provided that IRF-1 and IRF-2 manifest antionco...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Tanaka N,Kawakami T,Taniguchi T

    更新日期:1993-08-01 00:00:00

  • Endogenous fluctuations of DNA topology in the chloroplast of Chlamydomonas reinhardtii.

    abstract::DNA supercoiling in the chloroplast of the unicellular green alga Chlamydomonas reinhardtii was found to change with a diurnal rhythm in cells growing in alternating 12-h dark-12-h light periods. Highest and lowest DNA superhelicities occurred at the beginning and towards the end of the 12-h light periods, respectivel...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Salvador ML,Klein U,Bogorad L

    更新日期:1998-12-01 00:00:00

  • SSN20 is an essential gene with mutant alleles that suppress defects in SUC2 transcription in Saccharomyces cerevisiae.

    abstract::Dominant and recessive mutations at the SSN20 locus were previously isolated as extragenic suppressors of mutations in three genes (SNF2, SNF5, and SNF6) that are required in trans to derepress invertase expression. All ssn20 alleles cause recessive, temperature-sensitive lethality. In this study we cloned the SSN20 g...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Neigeborn L,Celenza JL,Carlson M

    更新日期:1987-02-01 00:00:00

  • Potentiation of protein kinase C zeta activity by 15-deoxy-delta(12,14)-prostaglandin J(2) induces an imbalance between mitogen-activated protein kinases and NF-kappa B that promotes apoptosis in macrophages.

    abstract::Activation of the macrophage cell line RAW 264.7 with lipopolysaccharide (LPS) transiently activates protein kinase C zeta (PKC zeta) and Jun N-terminal kinase (JNK) through a phosphoinositide-3-kinase (PI3-kinase)-dependent pathway. Incubation of LPS-treated cells with the cyclopentenone 15-deoxy-Delta(12,14)-prostag...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Castrillo A,Través PG,Martín-Sanz P,Parkinson S,Parker PJ,Boscá L

    更新日期:2003-02-01 00:00:00

  • Rb/E2F1 regulates the innate immune receptor Toll-like receptor 3 in epithelial cells.

    abstract::Tumor suppressor genes regulate the antiviral host defense through molecular mechanisms that are not yet well explored. Here, we show that the tumor suppressor retinoblastoma (Rb) protein positively regulates Toll-like receptor 3 (TLR3) expression, the sensing receptor for viral double-stranded RNA and poly(I · C). TL...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Taura M,Suico MA,Koyama K,Komatsu K,Miyakita R,Matsumoto C,Kudo E,Kariya R,Goto H,Kitajima S,Takahashi C,Shuto T,Nakao M,Okada S,Kai H

    更新日期:2012-04-01 00:00:00

  • DNA methylation associated with repeat-induced point mutation in Neurospora crassa.

    abstract::Repeat-induced point mutation (RIP) is a process that efficiently detects DNA duplications prior to meiosis in Neurospora crassa and peppers them with G:C to A:T mutations. Cytosine methylation is typically associated with sequences affected by RIP, and methylated cytosines are not limited to CpG dinucleotides. We gen...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Singer MJ,Marcotte BA,Selker EU

    更新日期:1995-10-01 00:00:00

  • Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.

    abstract::Unlike Saccharomyces cerevisiae RNA polymerase III, human RNA polymerase III has not been entirely characterized. Orthologues of the yeast RNA polymerase III subunits C128 and C37 remain unidentified, and for many of the other subunits, the available information is limited to database sequences with various degrees of...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hu P,Wu S,Sun Y,Yuan CC,Kobayashi R,Myers MP,Hernandez N

    更新日期:2002-11-01 00:00:00

  • Three functional classes of transcriptional activation domain.

    abstract::We have studied the abilities of different transactivation domains to stimulate the initiation and elongation (postinitiation) steps of RNA polymerase II transcription in vivo. Nuclear run-on and RNase protection analyses revealed three classes of activation domains: Sp1 and CTF stimulated initiation (type I); human i...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Blau J,Xiao H,McCracken S,O'Hare P,Greenblatt J,Bentley D

    更新日期:1996-05-01 00:00:00

  • DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis.

    abstract::Nucleosomes were reconstituted in vitro with several singly end-labeled restriction fragments derived from a cloned somatic-type 5S RNA gene of Xenopus laevis and purified nucleosome core particles from Xenopus cultured cells or chicken erythrocytes. Nucleosome locations were determined by digestion of the reconstitut...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Gottesfeld JM

    更新日期:1987-05-01 00:00:00

  • ZNF536, a novel zinc finger protein specifically expressed in the brain, negatively regulates neuron differentiation by repressing retinoic acid-induced gene transcription.

    abstract::Neuronal differentiation is tightly regulated by a variety of factors. In a search for neuron-specific genes, we identified a highly conserved novel zinc finger protein, ZNF536. We observed that ZNF536 is most abundant in the brain and, in particular, is expressed in the developing central nervous system and dorsal ro...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Qin Z,Ren F,Xu X,Ren Y,Li H,Wang Y,Zhai Y,Chang Z

    更新日期:2009-07-01 00:00:00

  • Phenotypic analysis of Meltrin alpha (ADAM12)-deficient mice: involvement of Meltrin alpha in adipogenesis and myogenesis.

    abstract::Meltrin alpha (ADAM12) is a metalloprotease-disintegrin whose specific expression patterns during development suggest that it is involved in myogenesis and the development of other organs. To determine the roles Meltrin alpha plays in vivo, we generated Meltrin alpha-deficient mice by gene targeting. Although the numb...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kurisaki T,Masuda A,Sudo K,Sakagami J,Higashiyama S,Matsuda Y,Nagabukuro A,Tsuji A,Nabeshima Y,Asano M,Iwakura Y,Sehara-Fujisawa A

    更新日期:2003-01-01 00:00:00

  • Miz1 is required for early embryonic development during gastrulation.

    abstract::Miz1 is a member of the POZ domain/zinc finger transcription factor family. In vivo, Miz1 forms a complex with the Myc oncoprotein and recruits Myc to core promoter elements. Myc represses transcription through Miz1 binding sites. We now show that the Miz1 gene is ubiquitously expressed during mouse embryogenesis. In ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Adhikary S,Peukert K,Karsunky H,Beuger V,Lutz W,Elsässer HP,Möröy T,Eilers M

    更新日期:2003-11-01 00:00:00

  • Species-specific elements in the large T-antigen J domain are required for cellular transformation and DNA replication by simian virus 40.

    abstract::The J domain of simian virus 40 (SV40) large T antigen is required for efficient DNA replication and transformation. Despite previous reports demonstrating the promiscuity of J domains in heterologous systems, results presented here show the requirement for specific J-domain sequences in SV40 large-T-antigen-mediated ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sullivan CS,Tremblay JD,Fewell SW,Lewis JA,Brodsky JL,Pipas JM

    更新日期:2000-08-01 00:00:00

  • Roles of hnRNP A1, SR proteins, and p68 helicase in c-H-ras alternative splicing regulation.

    abstract::Human ras genes play central roles in coupling extracellular signals with complex intracellular networks controlling proliferation, differentiation, and apoptosis, among others processes. c-H-ras pre-mRNA can be alternatively processed into two mRNAs due to the inclusion or exclusion of the alternative exon IDX; this ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Guil S,Gattoni R,Carrascal M,Abián J,Stévenin J,Bach-Elias M

    更新日期:2003-04-01 00:00:00

  • MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties.

    abstract::The human genome contains a number of methyl CpG binding proteins that translate DNA methylation into a physiological response. To gain insight into the function of MBD2 and MBD3, we first applied protein tagging and mass spectrometry. We show that MBD2 and MBD3 assemble into mutually exclusive distinct Mi-2/NuRD-like...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Le Guezennec X,Vermeulen M,Brinkman AB,Hoeijmakers WA,Cohen A,Lasonder E,Stunnenberg HG

    更新日期:2006-02-01 00:00:00

  • Transcriptional and posttranscriptional regulation of the proliferating cell nuclear antigen gene.

    abstract::The steady-state mRNA levels of the proliferating cell nuclear antigen (PCNA) gene are growth regulated. In a previous paper (L. Ottavio, C.-D. Chang, M. G. Rizzo, S. Travali, C. Casadevall, and R. Baserga, Mol. Cell. Biol. 10:303-309, 1990), we reported that introns (especially intron 4) participate in growth regulat...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Chang CD,Ottavio L,Travali S,Lipson KE,Baserga R

    更新日期:1990-07-01 00:00:00

  • Enhanced production of hepatitis B surface antigen in NIH 3T3 mouse fibroblasts by using extrachromosomally replicating bovine papillomavirus vector.

    abstract::We have constructed a recombinant pBR322 plasmid composed of a subgenomic transforming fragment of bovine papillomavirus DNA and the hepatitis B surface antigen gene from cloned hepatitis B virus DNA and used it for transfection of NIH 3T3 mouse fibroblasts. The transformed cells retain the plasmids in extrachromosoma...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Wang Y,Stratowa C,Schaefer-Ridder M,Doehmer J,Hofschneider PH

    更新日期:1983-06-01 00:00:00

  • The emerging role of HP1 in the DNA damage response.

    abstract::Heterochromatin protein 1 (HP1) family members are versatile proteins involved in transcription, chromatin organization, and replication. Recent findings now have implicated HP1 proteins in the DNA damage response as well. Cell-biological approaches showed that reducing the levels of all three HP1 isoforms enhances DN...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章,评审


    authors: Dinant C,Luijsterburg MS

    更新日期:2009-12-01 00:00:00

  • Dual regulation of c-Myc by p300 via acetylation-dependent control of Myc protein turnover and coactivation of Myc-induced transcription.

    abstract::The c-Myc oncoprotein (Myc) controls cell fate by regulating gene transcription in association with a DNA-binding partner, Max. While Max lacks a transcription regulatory domain, the N terminus of Myc contains a transcription activation domain (TAD) that recruits cofactor complexes containing the histone acetyltransfe...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Faiola F,Liu X,Lo S,Pan S,Zhang K,Lymar E,Farina A,Martinez E

    更新日期:2005-12-01 00:00:00

  • The simian virus 40 sequences between 0.169 and 0.423 map units are not essential to immortalize early-passage rat embryo cells.

    abstract::F8dl is a simian virus 40 early-region deletion mutant that lacks the sequences between 0.169 and 0.423 map units. We show that cloned F8dl DNA immortalized early-passage Fisher rat embryo cells with an efficiency that was about 20% of that of cloned wild-type simian virus 40 DNA. In contrast, we detected no immortali...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sompayrac L,Danna KJ

    更新日期:1985-05-01 00:00:00

  • Evidence for an overlapping role of CLOCK and NPAS2 transcription factors in liver circadian oscillators.

    abstract::The mechanisms underlying the circadian control of gene expression in peripheral tissues and influencing many biological pathways are poorly defined. Factor VII (FVII), the protease triggering blood coagulation, represents a valuable model to address this issue in liver since its plasma levels oscillate in a circadian...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bertolucci C,Cavallari N,Colognesi I,Aguzzi J,Chen Z,Caruso P,Foá A,Tosini G,Bernardi F,Pinotti M

    更新日期:2008-05-01 00:00:00

  • Synergy between Apc min and an activated ras mutation is sufficient to induce colon carcinomas.

    abstract::Colon carcinomas appear to arise from the cumulative effect of mutations to several genes (APC, DCC, p53, ras, hMLH1, and hMSH2). By using novel colonic epithelial cell lines derived from the Immorto mouse, named the YAMC (young adult mouse colon) cell line, and an Immorto-Min mouse hybrid, named the IMCE (Immorto-Min...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: D'Abaco GM,Whitehead RH,Burgess AW

    更新日期:1996-03-01 00:00:00

  • A severely defective TATA-binding protein-TFIIB interaction does not preclude transcriptional activation in vivo.

    abstract::In yeast cells, mutations in the TATA-binding protein (TBP) that disrupt the interaction with the TATA element or with TFIIA can selectively impair the response to acidic activator proteins. We analyzed the transcriptional properties of TBP derivatives in which residues that directly interact with TFIIB were replaced ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Lee M,Struhl K

    更新日期:1997-03-01 00:00:00

  • SIRT4 represses peroxisome proliferator-activated receptor α activity to suppress hepatic fat oxidation.

    abstract::Sirtuins are a family of protein deacetylases, deacylases, and ADP-ribosyltransferases that regulate life span, control the onset of numerous age-associated diseases, and mediate metabolic homeostasis. We have uncovered a novel role for the mitochondrial sirtuin SIRT4 in the regulation of hepatic lipid metabolism duri...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Laurent G,de Boer VC,Finley LW,Sweeney M,Lu H,Schug TT,Cen Y,Jeong SM,Li X,Sauve AA,Haigis MC

    更新日期:2013-11-01 00:00:00

  • Genetic interaction between transcription elongation factor TFIIS and RNA polymerase II.

    abstract::Little is known about the regions of RNA polymerase II (RNAPII) that are involved in the process of transcript elongation and interaction with elongation factors. One elongation factor, TFIIS, stimulates transcript elongation by binding to RNAPII and facilitating its passage through intrinsic pausing sites in vitro. I...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Archambault J,Lacroute F,Ruet A,Friesen JD

    更新日期:1992-09-01 00:00:00