The MES-1 murine enhancer element is closely associated with the heterogeneous 5' ends of two divergent transcription units.

abstract：:Sortilin is a member of the Vps10p domain family of neuropeptide and neurotrophin binding neuronal receptors. The family members interact with and partly share a variety of ligands and partake in intracellular sorting and protein transport as well as in transmembrane signal transduction. Thus, sortilin mediates the tr...

journal_title：Molecular and cellular biology

authors： Larsen JV,Hansen M,Møller B,Madsen P,Scheller J,Nielsen M,Petersen CM

更新日期：2010-09-01 00:00:00

abstract：:We examined the mechanisms that control the downregulation of the c-myc mRNA during differentiation of HL60 cells. On treatment with dimethyl sulfoxide, HL60 cells downmodulated their steady-state c-myc message levels, ceased to proliferate, and underwent terminal differentiation. In nuclear run-on assays in which dis...

journal_title：Molecular and cellular biology

authors： Siebenlist U,Bressler P,Kelly K

更新日期：1988-02-01 00:00:00

abstract：:G proteins are molecular switches that control a wide variety of physiological functions, including neurotransmission, transcriptional activation, cell migration, cell growth. and proliferation. The ability of GTPases to participate in signaling events is determined by the ratio of GTP-bound to GDP-bound forms in the ...

journal_title：Molecular and cellular biology

authors： Xia C,Ma W,Stafford LJ,Liu C,Gong L,Martin JF,Liu M

更新日期：2003-04-01 00:00:00

abstract：:Expression of the recombinant human U1-70K protein in COS cells resulted in its rapid transport to the nucleus, even when binding to U1 RNA was debilitated. Deletion analysis of the U1-70K protein revealed the existence of two segments of the protein which were independently capable of nuclear localization. One nuclea...

journal_title：Molecular and cellular biology

authors： Romac JM,Graff DH,Keene JD

更新日期：1994-07-01 00:00:00

abstract：:Human insulin-like growth factor II (IGF-II) mRNAs are subject to site-specific endonucleolytic cleavage in the 3' untranslated region, leading to an unstable 5' cleavage product containing the IGF-II coding region and a very stable 3' cleavage product of 1.8 kb. This endonucleolytic cleavage is most probably the firs...

journal_title：Molecular and cellular biology

authors： Scheper W,Meinsma D,Holthuizen PE,Sussenbach JS

更新日期：1995-01-01 00:00:00

abstract：:Among the tumor necrosis factor (TNF) family of cytokines, FasL and TNF-related apoptosis-inducing ligand (TRAIL) are known to induce cell death via caspase activation. Recently, other biological functions of these death ligands have been postulated in vitro and in vivo. It was previously shown that Fas ligation induc...

journal_title：Molecular and cellular biology

authors： Choi C,Kutsch O,Park J,Zhou T,Seol DW,Benveniste EN

更新日期：2002-02-01 00:00:00

abstract：:The TIS11 primary response gene is rapidly and transiently induced by both 12-O-tetradecanoylphorbol-13-acetate and growth factors. The predicted TIS11 protein contains a 6-amino-acid repeat, YKTELC. We cloned two additional cDNAs, TIS11b and TIS11d, that contain the YKTELC sequence. TIS11, TIS11b, and TIS11d proteins...

journal_title：Molecular and cellular biology

authors： Varnum BC,Ma QF,Chi TH,Fletcher B,Herschman HR

更新日期：1991-03-01 00:00:00

abstract：:Transcriptional activation by nuclear hormone receptors is mediated by the 160-kDa family of nuclear receptor coactivators. These coactivators associate with DNA-bound nuclear receptors and transmit activating signals to the transcription machinery through two activation domains. In screening for mammalian proteins th...

journal_title：Molecular and cellular biology

authors： Huang SM,Stallcup MR

更新日期：2000-03-01 00:00:00

abstract：:B-lymphocyte development requires the basic helix-loop-helix proteins encoded by the E2A gene. In this study, the control mechanism of E2A was further explored by disruption of the E2A-related genes, E2-2 and HEB. In contrast to E2A, E2-2 and HEB are not essential for the establishment of the B-cell lineage. However, ...

journal_title：Molecular and cellular biology

authors： Zhuang Y,Cheng P,Weintraub H

更新日期：1996-06-01 00:00:00

abstract：:Rats and mice have two, equally expressed, nonallelic genes encoding preproinsulin (genes I and II). Cytological hybridization with metaphase chromosomes indicated that both genes reside on rat chromosome I but are approximately 100,000 kilobases apart. In mice the two genes reside on two different chromosomes. DNA se...

journal_title：Molecular and cellular biology

authors： Soares MB,Schon E,Henderson A,Karathanasis SK,Cate R,Zeitlin S,Chirgwin J,Efstratiadis A

更新日期：1985-08-01 00:00:00

abstract：:COUP-TFII has an important role in regulating metabolism in vivo. We showed this previously by deleting COUP-TFII from pancreatic beta cells in heterozygous mutant mice, which led to abnormal insulin secretion. Here, we report that COUP-TFII expression is reduced in the pancreas and liver of mice refed with a carbohyd...

journal_title：Molecular and cellular biology

authors： Perilhou A,Tourrel-Cuzin C,Kharroubi I,Henique C,Fauveau V,Kitamura T,Magnan C,Postic C,Prip-Buus C,Vasseur-Cognet M

更新日期：2008-11-01 00:00:00

abstract：:We describe a two-step strategy to alter any mouse locus repeatedly and efficiently by direct positive selection. Using conventional targeting for the first step, a functional neo gene and a nonfunctional HPRT minigene (the "socket") are introduced into the genome of HPRT- embryonic stem (ES) cells close to the chosen...

journal_title：Molecular and cellular biology

authors： Detloff PJ,Lewis J,John SW,Shehee WR,Langenbach R,Maeda N,Smithies O

更新日期：1994-10-01 00:00:00

abstract：:The common complications in obesity and type 2 diabetes include hepatic steatosis and disruption of glucose-glycogen homeostasis, leading to hyperglycemia. Fatty acid translocase (FAT/CD36), whose expression is inducible in obesity, is known for its function in fatty acid uptake. Previous work by us and others suggest...

journal_title：Molecular and cellular biology

authors： Garbacz WG,Lu P,Miller TM,Poloyac SM,Eyre NS,Mayrhofer G,Xu M,Ren S,Xie W

更新日期：2016-10-13 00:00:00

abstract：:In heat-shocked tomato cell cultures, cytoplasmic heat shock granules (HSGs) are tightly associated with a specific subset of mRNAs coding mainly for the untranslated control proteins. This messenger ribonucleoprotein complex was banded in a CsCl gradient after fixation with formaldehyde (approximately 1.30 g/cm3). It...

journal_title：Molecular and cellular biology

authors： Nover L,Scharf KD,Neumann D

更新日期：1989-03-01 00:00:00

abstract：:Aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor, is known to mediate a wide variety of pharmacological and toxicological effects caused by polycyclic aromatic hydrocarbons. Recent studies have revealed that AhR is involved in the normal development and homeostasis of many organs. Here, we demo...

journal_title：Molecular and cellular biology

authors： Sekine H,Mimura J,Oshima M,Okawa H,Kanno J,Igarashi K,Gonzalez FJ,Ikuta T,Kawajiri K,Fujii-Kuriyama Y

更新日期：2009-12-01 00:00:00

abstract：:We recently identified Vav, the product of the vav proto-oncogene, as a guanine nucleotide exchange factor (GEF) for Ras. Vav is enzymatically activated by lymphocyte antigen receptor-coupled protein tyrosine kinases or independently by diglycerides. To further evaluate the physiological role of Vav, we assessed its G...

journal_title：Molecular and cellular biology

authors： Gulbins E,Coggeshall KM,Langlet C,Baier G,Bonnefoy-Berard N,Burn P,Wittinghofer A,Katzav S,Altman A

更新日期：1994-02-01 00:00:00

abstract：:We report the isolation and molecular characterization of the mouse grb2 gene. The product of this gene, the Grb2 protein, is highly related to the Caenorhabditis elegans sem-5 gene product and the human GRB2 protein and displays the same SH3-SH2-SH3 structural motifs. In situ hybridization studies revealed that the m...

journal_title：Molecular and cellular biology

authors： Suen KL,Bustelo XR,Pawson T,Barbacid M

更新日期：1993-09-01 00:00:00

abstract：:The p20K gene is induced in conditions of reversible growth arrest in chicken embryo fibroblasts (CEF). This expression is dependent on transcriptional activation and on a region of the promoter designated the quiescence-responsive unit (QRU). In this report, we describe the regulatory elements of the QRU responsible ...

journal_title：Molecular and cellular biology

authors： Kim S,Mao PL,Gagliardi M,Bédard PA

更新日期：1999-08-01 00:00:00

abstract：:Cells of the human embryonal carcinoma line NEC14 proliferate as densely packed clusters consisting of small, polygonal stem cells and do not express a detectable level of fibronectin (FN). Upon induction of differentiation by treatment with N,N'-hexamethylene bisacetamide (HMBA), the level of FN mRNA increased steepl...

journal_title：Molecular and cellular biology

authors： Suzuki M,Oda E,Nakajima T,Sekiya S,Oda K

更新日期：1998-05-01 00:00:00

abstract：:Mitochondria are indispensable organelles implicated in multiple aspects of cellular processes, including tumorigenesis. Heat shock proteins play a critical regulatory role in accurately delivering the nucleus-encoded proteins through membrane-bound presequence translocase (Tim23 complex) machinery. Although altered e...

journal_title：Molecular and cellular biology

authors： Sinha D,Srivastava S,Krishna L,D'Silva P

更新日期：2014-05-01 00:00:00

abstract：:The murine Igh locus has a 3' regulatory region (3' RR) containing four enhancers (hs3A, hs1,2, hs3B, and hs4) at DNase I-hypersensitive sites. The 3' RR exerts long-range effects on class switch recombination (CSR) to several isotypes through its control of germ line transcription. By measuring levels of acetylated h...

journal_title：Molecular and cellular biology

authors： Garrett FE,Emelyanov AV,Sepulveda MA,Flanagan P,Volpi S,Li F,Loukinov D,Eckhardt LA,Lobanenkov VV,Birshtein BK

更新日期：2005-02-01 00:00:00

abstract：:B-cell stimulatory factor 2 (BSF-2) is a lymphokine which induces the final maturation of B cells. BSF-2 acts on a variety of cells other than B cells, and moreover, expression of BSF-2 mRNA is detected in interleukin-1 beta-stimulated glioblastoma and astrocytoma cell lines. Here, we studied the function of BSF-2 on ...

journal_title：Molecular and cellular biology

authors： Satoh T,Nakamura S,Taga T,Matsuda T,Hirano T,Kishimoto T,Kaziro Y

更新日期：1988-08-01 00:00:00

abstract：:Peroxisomal PTS2-dependent matrix protein import starts with the recognition of the PTS2 targeting signal by the import receptor Pex7p. Subsequently, the formed Pex7p/cargo complex is transported from the cytosol to the peroxisomal docking complex, consisting of Pex13p and Pex14p. In Saccharomyces cerevisiae, the latt...

journal_title：Molecular and cellular biology

authors： Stein K,Schell-Steven A,Erdmann R,Rottensteiner H

更新日期：2002-09-01 00:00:00

abstract：:Compromised immunoregulation contributes to obesity and complications in metabolic pathogenesis. Here, we demonstrate that the nuclear factor of activated T cell (NFAT) group of transcription factors contributes to glucose and insulin homeostasis. Expression of two members of the NFAT family (NFATc2 and NFATc4) is ind...

journal_title：Molecular and cellular biology

authors： Yang TT,Suk HY,Yang X,Olabisi O,Yu RY,Durand J,Jelicks LA,Kim JY,Scherer PE,Wang Y,Feng Y,Rossetti L,Graef IA,Crabtree GR,Chow CW

更新日期：2006-10-01 00:00:00

abstract：:Costimulation of G protein-coupled receptors (GPCRs) may result in cross talk interactions between their downstream signaling pathways. Stimulation of GPCRs may also lead to cross talk regulation of receptor tyrosine kinase signaling and thereby to activation of mitogen-activated protein kinase (MAPK). In COS-7 cells,...

journal_title：Molecular and cellular biology

authors： Hanke S,Nürnberg B,Groll DH,Liebmann C

更新日期：2001-12-01 00:00:00

abstract：:Scaffold proteins have been established as important mediators of signal transduction specificity. The insulin receptor substrate (IRS) proteins represent a critical group of scaffold proteins that are required for signal transduction by the insulin receptor, including the activation of phosphatidylinositol 3 kinase. ...

journal_title：Molecular and cellular biology

authors： Standen CL,Kennedy NJ,Flavell RA,Davis RJ

更新日期：2009-09-01 00:00:00

abstract：:p63 is a member of the p53 tumor suppressor gene family, which regulates downstream target gene expression by binding to sequence-specific response elements similar to those of p53. By using oligonucleotide expression microarray analysis and analyzing the promoters of p63-induced genes, we have identified novel p63-sp...

journal_title：Molecular and cellular biology

authors： Osada M,Park HL,Nagakawa Y,Yamashita K,Fomenkov A,Kim MS,Wu G,Nomoto S,Trink B,Sidransky D

更新日期：2005-07-01 00:00:00

abstract：:Spinal muscular atrophy (SMA) is an inherited motor neuron disease caused by homozygous loss of the Survival Motor Neuron 1 (SMN1) gene. In the absence of SMN1, inefficient inclusion of exon 7 in transcripts from the nearly identical SMN2 gene results in ubiquitous SMN decrease but selective motor neuron degeneration....

journal_title：Molecular and cellular biology

authors： Ruggiu M,McGovern VL,Lotti F,Saieva L,Li DK,Kariya S,Monani UR,Burghes AH,Pellizzoni L

更新日期：2012-01-01 00:00:00

abstract：:TACC2 is a member of the transforming acidic coiled-coil-containing protein family and is associated with the centrosome-spindle apparatus during cell cycling. In vivo, the TACC2 gene is expressed in various splice forms predominantly in postmitotic tissues, including heart, muscle, kidney, and brain. Studies of human...

journal_title：Molecular and cellular biology

authors： Schuendeln MM,Piekorz RP,Wichmann C,Lee Y,McKinnon PJ,Boyd K,Takahashi Y,Ihle JN

更新日期：2004-07-01 00:00:00

abstract：:We have studied the mechanism by which 5'-flanking sequences modulate the in vitro transcription of eucaryotic tRNA genes. Using deletion and linker substitution mutagenesis, we have found that the 5'-flanking sequences responsible for the different in vitro transcription levels of three Drosophila tRNA5Asn genes are ...

journal_title：Molecular and cellular biology

authors： Lofquist AK,Garcia AD,Sharp SJ

更新日期：1988-10-01 00:00:00