Abstract:
:In heat-shocked tomato cell cultures, cytoplasmic heat shock granules (HSGs) are tightly associated with a specific subset of mRNAs coding mainly for the untranslated control proteins. This messenger ribonucleoprotein complex was banded in a CsCl gradient after fixation with formaldehyde (approximately 1.30 g/cm3). It contains all the heat shock proteins and most of the RNA applied to the gradient. During heat shock, a reversible aggregation of HSGs from 15S precursor particles can be shown. These pre-HSGs are not identical to the 19S plant prosomes. Ultrastructural analysis supports the ribonucleoprotein nature of HSGs and their composition of approximately 10-nm precursor particles. A model summarizes our results. It gives a reasonable explanation for the striking conservation of untranslated mRNAs during heat shock and may apply also to animal cells.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Nover L,Scharf KD,Neumann Ddoi
10.1128/mcb.9.3.1298subject
Has Abstractpub_date
1989-03-01 00:00:00pages
1298-308issue
3eissn
0270-7306issn
1098-5549journal_volume
9pub_type
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