Abstract:
:mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 (YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that the spb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detection of mRNA species trimmed from the 3' end. These data show that this Sm-like protein family member is involved in the process of mRNA decapping, and they provide an example of 3'-5' mRNA degradation intermediates in yeast.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Boeck R,Lapeyre B,Brown CE,Sachs ABdoi
10.1128/mcb.18.9.5062subject
Has Abstractpub_date
1998-09-01 00:00:00pages
5062-72issue
9eissn
0270-7306issn
1098-5549journal_volume
18pub_type
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