Differential recognition of response elements determines target gene specificity for p53 and p63.

abstract：:Although both osteoblasts and adipocytes have a common origin, i.e., mesenchymal cells, the molecular mechanisms that define the direction of two different lineages are presently unknown. In this study, we investigated the role of a transcription factor, CCAAT/enhancer binding protein beta (C/EBPbeta), and its isoform...

journal_title：Molecular and cellular biology

authors： Hata K,Nishimura R,Ueda M,Ikeda F,Matsubara T,Ichida F,Hisada K,Nokubi T,Yamaguchi A,Yoneda T

更新日期：2005-03-01 00:00:00

abstract：:Lactose or galactose induces the expression of the lactose-galactose regulon in Kluyveromyces lactis. We show here that the regulon is not induced in strains defective in LAC9. We demonstrate that this gene codes for a regulatory protein that acts in a positive manner to induce transcription. The LAC9 gene was isolate...

journal_title：Molecular and cellular biology

authors： Wray LV Jr,Witte MM,Dickson RC,Riley MI

更新日期：1987-03-01 00:00:00

abstract：:Here, we demonstrate that SENP2, a desumoylating enzyme, plays a critical role in the control of adipogenesis. SENP2 expression was markedly increased upon the induction of adipocyte differentiation, and this increase was dependent on protein kinase A activation. Remarkably, knockdown of SENP2 led to a dramatic attenu...

journal_title：Molecular and cellular biology

authors： Chung SS,Ahn BY,Kim M,Choi HH,Park HS,Kang S,Park SG,Kim YB,Cho YM,Lee HK,Chung CH,Park KS

更新日期：2010-05-01 00:00:00

abstract：:Notch signaling requires a series of proteolytic cleavage events to release the Notch intracellular domain (NICD) that functions directly in signal transduction. The Notch receptor is locked down in a protease-resistant state by a negative regulatory region (NRR) that protects an ADAM (a disintegrin and metalloproteas...

journal_title：Molecular and cellular biology

authors： Bozkulak EC,Weinmaster G

更新日期：2009-11-01 00:00:00

abstract：:MicroRNA 155 (miR-155) is an oncomir, generated as a noncoding RNA from the BIC gene whose promoter activity is mainly controlled via activation protein 1 (AP-1) and NF-κB transcription factors. We found that the expression levels of miR-155 and programmed cell death 4 (Pdcd4) exhibit inverse relationships in tongue c...

journal_title：Molecular and cellular biology

authors： Zargar S,Tomar V,Shyamsundar V,Vijayalakshmi R,Somasundaram K,Karunagaran D

更新日期：2019-03-01 00:00:00

abstract：:Poly(A) signals located at the 3' end of eukaryotic genes drive cleavage and polyadenylation at the same end of pre-mRNA. Although these sequences are expected only at the 3' end of genes, we found that strong poly(A) signals are also predicted within the 5' untranslated regions (UTRs) of many Drosophila melanogaster ...

journal_title：Molecular and cellular biology

authors： Guo J,Garrett M,Micklem G,Brogna S

更新日期：2011-02-01 00:00:00

abstract：:Nuclear microinjection of c-H-ras DNA induced DNA synthesis in reversibly nonproliferating quiescent human cells. The proto-oncogene and oncogene forms were equally effective inducers. In contrast, c-H-ras DNA either alone or in combination with the adenovirus E1A gene did not cause terminally nondividing senescent ce...

journal_title：Molecular and cellular biology

authors： Lumpkin CK,Knepper JE,Butel JS,Smith JR,Pereira-Smith OM

更新日期：1986-08-01 00:00:00

abstract：:Among the tumor necrosis factor (TNF) family of cytokines, FasL and TNF-related apoptosis-inducing ligand (TRAIL) are known to induce cell death via caspase activation. Recently, other biological functions of these death ligands have been postulated in vitro and in vivo. It was previously shown that Fas ligation induc...

journal_title：Molecular and cellular biology

authors： Choi C,Kutsch O,Park J,Zhou T,Seol DW,Benveniste EN

更新日期：2002-02-01 00:00:00

abstract：:Exposure of phosphatidylserine (PS) on the cell surface occurs early during apoptosis and serves as a recognition signal for phagocytes. Clearance of apoptotic cells by a membrane PS receptor is one of the critical anti-inflammatory functions of macrophages. However, the PS binding receptors and their recognition mech...

journal_title：Molecular and cellular biology

authors： Park SY,Kim SY,Jung MY,Bae DJ,Kim IS

更新日期：2008-09-01 00:00:00

abstract：:Inactivating mutations within the von Hippel-Lindau (VHL) tumor suppressor gene predispose patients to develop a variety of highly vascularized tumors. pVHL targets alpha subunits of the heterodimeric transcription factor hypoxia-inducible factor (HIF), a critical regulator of energy metabolism, angiogenesis, hematopo...

journal_title：Molecular and cellular biology

authors： Mack FA,Patel JH,Biju MP,Haase VH,Simon MC

更新日期：2005-06-01 00:00:00

abstract：:The heat shock transcription factors (Hsfs) activate the stress-inducible expression of heat shock proteins (Hsps) and other molecular chaperones in response to stress and, therefore, play an essential role in protein disaggregation and protein folding. In humans, missense mutation in the hsf4 gene causes cataract, an...

journal_title：Molecular and cellular biology

authors： Hu Y,Mivechi NF

更新日期：2006-04-01 00:00:00

abstract：:A cDNA sequence which contains the entire coding region for human purine nucleoside phosphorylase (PNP) was recombined for selection and expression in mammalian cells. Plasmids containing either the simian virus 40 early promoter or the mouse metallothionein promoter positioned just upstream of the PNP coding sequence...

journal_title：Molecular and cellular biology

authors： McIvor RS,Goddard JM,Simonsen CC,Martin DW Jr

更新日期：1985-06-01 00:00:00

abstract：:Misregulation of NF-kappaB signaling leads to infectious, inflammatory, or autoimmune disorders. IkappaB kinase beta (IKKbeta) is an essential activator of NF-kappaB and is known to phosphorylate the NF-kappaB inhibitor, IkappaBalpha, allowing it to undergo ubiquitin-mediated proteasomal degradation. However, beyond I...

journal_title：Molecular and cellular biology

authors： Hutti JE,Turk BE,Asara JM,Ma A,Cantley LC,Abbott DW

更新日期：2007-11-01 00:00:00

abstract：:NF-kappaB activation is reciprocally regulated by RelA/p65 acetylation and deacetylation, which are mediated by histone acetyltransferases (HATs) and deacetylases (HDACs). Here we demonstrate that in leukemia cells, NF-kappaB activation by the HDAC inhibitors (HDACIs) MS-275 and suberoylanilide hydroxamic acid was ass...

journal_title：Molecular and cellular biology

authors： Dai Y,Rahmani M,Dent P,Grant S

更新日期：2005-07-01 00:00:00

abstract：:Studies were performed to verify the physiological significance of attenuation in the life cycle of simian virus 40 and the role of agnoprotein in this process. For these purposes, nuclei were isolated at various times after infection and incubated in vitro in the presence of [alpha-32P]UTP under the standard conditio...

journal_title：Molecular and cellular biology

authors： Hay N,Aloni Y

更新日期：1985-06-01 00:00:00

abstract：:Six4 is a member of the Six family genes, homologues of Drosophila melanogaster sine oculis. The gene is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles. To elucidate t...

journal_title：Molecular and cellular biology

authors： Ozaki H,Watanabe Y,Takahashi K,Kitamura K,Tanaka A,Urase K,Momoi T,Sudo K,Sakagami J,Asano M,Iwakura Y,Kawakami K

更新日期：2001-05-01 00:00:00

abstract：:The NuA4 histone acetyltransferase (HAT) multisubunit complex is responsible for acetylation of histone H4 and H2A N-terminal tails in yeast. Its catalytic component, Esa1, is essential for cell cycle progression, gene-specific regulation and has been implicated in DNA repair. Almost all NuA4 subunits have clear homol...

journal_title：Molecular and cellular biology

authors： Doyon Y,Selleck W,Lane WS,Tan S,Côté J

更新日期：2004-03-01 00:00:00

abstract：:We have analyzed the fine structure of DNA repair in Chinese hamster ovary (CHO) cells within the G1 and G2 phases of the cell cycle. Repair of inactive regions of the genome has been suggested to increase in the G2 phase of the cell cycle compared with other phases. However, detailed studies of DNA repair in the G2 p...

journal_title：Molecular and cellular biology

authors： Petersen LN,Orren DK,Bohr VA

更新日期：1995-07-01 00:00:00

abstract：:To interrogate endogenous p21(WAF1/CIP1) (p21) promoter activity under basal conditions and in response to various forms of stress, knock-in imaging reporter mice in which expression of firefly luciferase (FLuc) was placed under the control of the endogenous p21 promoter within the Cdkn1a gene locus were generated. Bi...

journal_title：Molecular and cellular biology

authors： Tinkum KL,Marpegan L,White LS,Sun J,Herzog ED,Piwnica-Worms D,Piwnica-Worms H

更新日期：2011-09-01 00:00:00

abstract：:The Saccharomyces cerevisiae PGK (phosphoglycerate kinase) gene encodes one of the most abundant mRNA and protein species in the cell. To identify the promoter sequences required for the efficient expression of PGK, we undertook a detailed internal deletion analysis of the 5' noncoding region of the gene. Our analysis...

journal_title：Molecular and cellular biology

authors： Ogden JE,Stanway C,Kim S,Mellor J,Kingsman AJ,Kingsman SM

更新日期：1986-12-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) Tat protein activates transcription elongation by stimulating the Tat-activated kinase (TAK/p-TEFb), a protein kinase composed of CDK9 and its cyclin partner, cyclin T1. CDK9 is able to hyperphosphorylate the carboxyl-terminal domain (CTD) of the large subunit of RNA pol...

journal_title：Molecular and cellular biology

authors： Bourgeois CF,Kim YK,Churcher MJ,West MJ,Karn J

更新日期：2002-02-01 00:00:00

abstract：:Protein acetylation has emerged as a means of controlling levels of mRNA synthesis in eukaryotic cells. Here we report that acetyl coenzyme A (acetyl-CoA) stimulates RNA polymerase II transcription in vitro in the absence of histones. The effect of acetyl-CoA on basal and activated transcription was studied in a human...

journal_title：Molecular and cellular biology

authors： Galasinski SK,Lively TN,Grebe De Barron A,Goodrich JA

更新日期：2000-03-01 00:00:00

abstract：:Telomerase synthesizes tandem repeats of the sequence d(TTGGGG) onto input d(TTGGGG)n primer oligonucleotides (C. W. Greider and E. H. Blackburn, Cell 43:405-413). An intrinsic RNA component of the enzyme provides the template for d(TTGGGG)n repeat synthesis [C. W. Greider and E. H. Blackburn, Nature (London) 337:331-...

journal_title：Molecular and cellular biology

authors： Greider CW

更新日期：1991-09-01 00:00:00

abstract：:BCS1, a component of the inner membrane of mitochondria, belongs to the group of proteins with internal, noncleavable import signals. Import and intramitochondrial sorting of BCS1 are encoded in the N-terminal 126 amino acid residues. Three sequence elements were identified in this region, namely, the transmembrane do...

journal_title：Molecular and cellular biology

authors： Stan T,Brix J,Schneider-Mergener J,Pfanner N,Neupert W,Rapaport D

更新日期：2003-04-01 00:00:00

abstract：:The protein kinase Akt plays a critical role in heart function and is activated by phosphorylation of threonine 308 (T308) and serine 473 (S473). While phosphoinositide-dependent kinase 1 (PDK1) is responsible for Akt T308 phosphorylation, the identities of the kinases for Akt S473 phosphorylation in the heart remain ...

journal_title：Molecular and cellular biology

authors： Zhao X,Lu S,Nie J,Hu X,Luo W,Wu X,Liu H,Feng Q,Chang Z,Liu Y,Cao Y,Sun H,Li X,Hu Y,Yang Z

更新日期：2014-06-01 00:00:00

abstract：:We constructed a series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells. The prototype recombinant in this series, pSV2-cat, consisted of the beta-lactamase gene and origin of replication from pBR322 coupled to a simian virus 40 (SV40) early tra...

journal_title：Molecular and cellular biology

authors： Gorman CM,Moffat LF,Howard BH

更新日期：1982-09-01 00:00:00

abstract：:The enhancer of yellow 1 gene, e(y)1, of Drosophila melanogaster has been cloned and demonstrated to encode the TAFII40 protein. The e(y)1 gene is expressed in females much more strongly than in males due to the accumulation of e(y)1 mRNA in the ovaries. Two different e(y)1 mutations have been obtained. The e(y)1(ul) ...

journal_title：Molecular and cellular biology

authors： Soldatov A,Nabirochkina E,Georgieva S,Belenkaja T,Georgiev P

更新日期：1999-05-01 00:00:00

abstract：:Chondromodulin I (chm-I), a type II transmembrane protein, is highly expressed in the avascular zones of cartilage but is downregulated in the hypertrophic region, which is invaded by blood vessels during enchondral ossification. In vitro and in vivo assays with the purified protein have shown chondrocyte-modulating a...

journal_title：Molecular and cellular biology

authors： Brandau O,Aszódi A,Hunziker EB,Neame PJ,Vestweber D,Fässler R

更新日期：2002-09-01 00:00:00

abstract：:Allelic forms of DRG1/AFG2 confer resistance to the drug diazaborine, an inhibitor of ribosome biogenesis in Saccharomyces cerevisiae. Our results show that the AAA-ATPase Drg1 is essential for 60S maturation and associates with 60S precursor particles in the cytoplasm. Functional inactivation of Drg1 leads to an incr...

journal_title：Molecular and cellular biology

authors： Pertschy B,Saveanu C,Zisser G,Lebreton A,Tengg M,Jacquier A,Liebminger E,Nobis B,Kappel L,van der Klei I,Högenauer G,Fromont-Racine M,Bergler H

更新日期：2007-10-01 00:00:00

abstract：:In the early phase of bone damage, low concentrations of the cytokine tumor necrosis factor alpha (TNF-α) favor osteoblast differentiation. In contrast, chronic high doses of the same cytokine contribute to bone loss, demonstrating opposite effects depending on its concentration and on the time of exposure. In the bon...

journal_title：Molecular and cellular biology

authors： Daniele S,Natali L,Giacomelli C,Campiglia P,Novellino E,Martini C,Trincavelli ML

更新日期：2017-03-31 00:00:00