Chondromodulin I is dispensable during enchondral ossification and eye development.

abstract：:The interaction of Prep1 and Pbx homeodomain transcription factors regulates their activity, nuclear localization, and likely, function in development. To understand the in vivo role of Prep1, we have analyzed an embryonic lethal hypomorphic mutant mouse (Prep1(i/i)). Prep1(i/i) embryos die at embryonic day 17.5 (E17....

journal_title：Molecular and cellular biology

authors： Ferretti E,Villaescusa JC,Di Rosa P,Fernandez-Diaz LC,Longobardi E,Mazzieri R,Miccio A,Micali N,Selleri L,Ferrari G,Blasi F

更新日期：2006-08-01 00:00:00

abstract：:A special group of mitochondrial outer membrane proteins spans the membrane once, exposing soluble domains to both sides of the membrane. These proteins are synthesized in the cytosol and then inserted into the membrane by an unknown mechanism. To identify proteins that are involved in the biogenesis of the single-spa...

journal_title：Molecular and cellular biology

authors： Papić D,Elbaz-Alon Y,Koerdt SN,Leopold K,Worm D,Jung M,Schuldiner M,Rapaport D

更新日期：2013-10-01 00:00:00

abstract：:The elongation of transcription is a highly regulated process that requires negative and positive effectors. By binding the double-stranded stem in the transactivation response (TAR) element, RD protein from the negative transcription elongation factor (NELF) inhibits basal transcription from the long terminal repeat ...

journal_title：Molecular and cellular biology

authors： Fujinaga K,Irwin D,Huang Y,Taube R,Kurosu T,Peterlin BM

更新日期：2004-01-01 00:00:00

abstract：:The protein tyrosine kinase c-Src is regulated by two intramolecular interactions. The repressed state is achieved through the interaction of the Src homology 2 (SH2) domain with the phosphorylated C-terminal tail and the association of the SH3 domain with a polyproline type II helix formed by the linker region betwee...

journal_title：Molecular and cellular biology

authors： Adolph D,Flach N,Mueller K,Ostareck DH,Ostareck-Lederer A

更新日期：2007-03-01 00:00:00

abstract：:Activation of canonical Wnt signaling inhibits brown adipogenesis of cultured cells by impeding induction of PPARgamma and C/EBPalpha. Although enforced expression of these adipogenic transcription factors restores lipid accumulation and expression of FABP4 in Wnt-expressing cells, additional expression of PGC-1alpha ...

journal_title：Molecular and cellular biology

authors： Kang S,Bajnok L,Longo KA,Petersen RK,Hansen JB,Kristiansen K,MacDougald OA

更新日期：2005-02-01 00:00:00

abstract：:A truncated H-2Ld gene was constructed by deleting the transmembrane and cytoplasmic exons. The truncated H-2Ld gene was introduced into mouse L cells using the thymidine kinase gene as a selectable marker. Transformants were isolated and screened for the presence of truncated H-2Ld antigen. The truncated H-2Ld gene p...

journal_title：Molecular and cellular biology

authors： Murre C,Parker KC,Reiss CS,Driller JP,Wiley DC,Burakoff SJ,Seidman JG

更新日期：1986-04-01 00:00:00

abstract：:The muscle LIM protein (MLP) and cofilin 2 (CFL2) are important regulators of striated myocyte function. Mutations in the corresponding genes have been directly associated with severe human cardiac and skeletal myopathies, and aberrant expression patterns have often been observed in affected muscles. Herein, we have i...

journal_title：Molecular and cellular biology

authors： Papalouka V,Arvanitis DA,Vafiadaki E,Mavroidis M,Papadodima SA,Spiliopoulou CA,Kremastinos DT,Kranias EG,Sanoudou D

更新日期：2009-11-01 00:00:00

abstract：:alpha 1-Antitrypsin (AT), the major elastase inhibitor in mammalian serum, is produced primarily in the liver. We have characterized AT gene structure and expression in the mouse species Mus caroli, which expresses high levels of AT in the kidneys as well as in the liver. Analysis of cDNA and genomic clones showed tha...

journal_title：Molecular and cellular biology

authors： Latimer JJ,Berger FG,Baumann H

更新日期：1990-02-01 00:00:00

abstract：:Telomerase promotes telomere maintenance by copying a template within its integral RNA subunit to elongate chromosome ends with new telomeric repeats. Motifs have been defined within the telomerase RNA that contribute to mature RNA accumulation, holoenzyme catalytic activity, or enzyme recruitment to telomeres. Here, ...

journal_title：Molecular and cellular biology

authors： Sexton AN,Collins K

更新日期：2011-02-01 00:00:00

abstract：:Numerous studies indirectly implicate Rac GTPases in cancer. To investigate if Rac3 contributes to normal or malignant cell function, we generated rac3 null mutants through gene targeting. These mice were viable, fertile, and lacked an obvious external phenotype. This shows Rac3 function is dispensable for embryonic d...

journal_title：Molecular and cellular biology

authors： Cho YJ,Zhang B,Kaartinen V,Haataja L,de Curtis I,Groffen J,Heisterkamp N

更新日期：2005-07-01 00:00:00

abstract：:The product of the c-myc proto-oncogene is an important positive regulator of cell growth and proliferation. Recently, c-Myc has also been demonstrated to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. To further examine Myc-induced apoptosis, we coexpressed the proto-oncoge...

journal_title：Molecular and cellular biology

authors： Wagner AJ,Small MB,Hay N

更新日期：1993-04-01 00:00:00

abstract：:The GLN3 gene of Saccharomyces cerevisiae is required for the activation of transcription of a number of genes in response to the replacement of glutamine by glutamate as source of nitrogen. We cloned the GLN3 gene and constructed null alleles by gene disruption. GLN3 is not essential for growth, but increased copies ...

journal_title：Molecular and cellular biology

authors： Minehart PL,Magasanik B

更新日期：1991-12-01 00:00:00

abstract：:Mos is a germ cell-specific serine/threonine protein kinase that activates mitogen-activated protein kinase (MAPK) through MAPK kinase (MKK). In Xenopus oocytes, Mos synthesis is required for progesterone-induced activation of MAPK and maturation promoting factor. Injection of Mos or active MAPK causes mitotic arrest ...

journal_title：Molecular and cellular biology

authors： Chen M,Cooper JA

更新日期：1995-09-01 00:00:00

abstract：:Ras GTPases were long thought to function exclusively from the plasma membrane (PM). However, a current model suggests that Ras proteins can compartmentalize to regulate different functions, and an oncogenic H-Ras mutant that is restricted to the endomembrane can still transform cells. In this study, we demonstrated t...

journal_title：Molecular and cellular biology

authors： Cheng CM,Li H,Gasman S,Huang J,Schiff R,Chang EC

更新日期：2011-03-01 00:00:00

abstract：:The locus control region (LCR) regulates transcription of the downstream beta-like globin genes 10 to 50 kb away. Among hypersensitive sites HS4, -3, -2, and -1, which define the LCR in erythroid cells, HS2 possesses prominent enhancer function. The mechanism by which the HS2 enhancer and other functional components o...

journal_title：Molecular and cellular biology

authors： Kong S,Bohl D,Li C,Tuan D

更新日期：1997-07-01 00:00:00

abstract：:The upstream activating sequence of the adjacent and divergently transcribed GAL1 and GAL10 genes of Saccharomyces cerevisiae (UASG) contains at least three distinct classes of overlapping transcriptional control sites. The transcription activator GAL4 binds to four related sites in UASG and induces expression of GAL1...

journal_title：Molecular and cellular biology

authors： Finley RL Jr,West RW Jr

更新日期：1989-10-01 00:00:00

abstract：:Telomerase extends chromosome ends by the synthesis of tandem simple-sequence repeats. Studies of minimal recombinant telomerase ribonucleoprotein (RNP) reconstituted in vitro have revealed sequences within the telomerase RNA subunit (TER) that are required to establish its internal template and other unique features ...

journal_title：Molecular and cellular biology

authors： Cunningham DD,Collins K

更新日期：2005-06-01 00:00:00

abstract：:Correct reprogramming of epigenetic marks in the donor nuclei is crucial for successful cloning by nuclear transfer. Specific epigenetic modifications, such as repressive histone lysine methylation marks, are known to be very stable and difficult to reprogram. The discovery of histone lysine demethylases has opened up...

journal_title：Molecular and cellular biology

authors： Antony J,Oback F,Chamley LW,Oback B,Laible G

更新日期：2013-03-01 00:00:00

abstract：:The eukaryotic Spt4-Spt5 heterodimer forms a higher-order complex with RNA polymerase II (and I) to regulate transcription elongation. Extensive genetic and functional data have revealed diverse roles of Spt4-Spt5 in coupling elongation with chromatin modification and RNA-processing pathways. A mechanistic understandi...

journal_title：Molecular and cellular biology

authors： Meyer PA,Li S,Zhang M,Yamada K,Takagi Y,Hartzog GA,Fu J

更新日期：2015-10-01 00:00:00

abstract：:The tumor suppressor p21 acts as a cell cycle inhibitor and has also been shown to regulate gene expression by functioning as a transcription corepressor. Here, we identified p21-regulated microRNAs (miRNAs) by sequencing small RNAs from isogenic p21(+/+) and p21(-/-) cells. Three abundant miRNA clusters, miR-200b-200...

journal_title：Molecular and cellular biology

authors： Li XL,Hara T,Choi Y,Subramanian M,Francis P,Bilke S,Walker RL,Pineda M,Zhu Y,Yang Y,Luo J,Wakefield LM,Brabletz T,Park BH,Sharma S,Chowdhury D,Meltzer PS,Lal A

更新日期：2014-02-01 00:00:00

abstract：:Genomic DNA in higher eucaryotic cells is organized into a series of loops, each of which may be affixed at its base to the nuclear matrix via a specific matrix attachment region (MAR). In this report, we describe the distribution of MARs within the amplified dihydrofolate reductase (DHFR) domain (amplicon) in the met...

journal_title：Molecular and cellular biology

authors： Dijkwel PA,Hamlin JL

更新日期：1988-12-01 00:00:00

abstract：:The inducible transcriptional complex AP-1, composed of c-Fos and c-Jun proteins, is crucial for cell adaptation to many environmental changes. While its mechanisms of activation have been extensively studied, how its activity is restrained is poorly understood. We report here that lysine 265 of c-Fos is conjugated by...

journal_title：Molecular and cellular biology

authors： Bossis G,Malnou CE,Farras R,Andermarcher E,Hipskind R,Rodriguez M,Schmidt D,Muller S,Jariel-Encontre I,Piechaczyk M

更新日期：2005-08-01 00:00:00

abstract：:The loss of p53 induces spontaneous tumors in mice, and p53 mutations are found in approximately 50% of human tumors. These tumors are generally caused by a number of events, including genomic instability, checkpoint defects, mitotic defects, deregulation of transcriptional targets, impaired apoptosis, and G(1) deregu...

journal_title：Molecular and cellular biology

authors： Padmakumar VC,Aleem E,Berthet C,Hilton MB,Kaldis P

更新日期：2009-05-01 00:00:00

abstract：:The adenovirus early-region 1B 19,000-molecular-weight tumor antigen is required for oncogenic transformation of cells by adenovirus. We have demonstrated that this tumor antigen is located in the nuclear envelope of infected and transformed cells and that a fraction of the protein within the nuclear envelope is assoc...

journal_title：Molecular and cellular biology

authors： White E,Blose SH,Stillman BW

更新日期：1984-12-01 00:00:00

abstract：:A 249-nucleotide coding region instability determinant (CRD) destabilizes c-myc mRNA. Previous experiments identified a CRD-binding protein (CRD-BP) that appears to protect the CRD from endonuclease cleavage. However, it was unclear why a CRD-BP is required to protect a well-translated mRNA whose coding region is cove...

journal_title：Molecular and cellular biology

authors： Lemm I,Ross J

更新日期：2002-06-01 00:00:00

abstract：:The Saccharomyces cerevisiae Trf4 and Trf5 proteins are members of a distinct family of eukaryotic DNA polymerase beta-like nucleotidyltransferases, and a template-dependent DNA polymerase activity has been reported for Trf4. To define the nucleotidyltransferase activities associated with Trf4 and Tr5, we purified the...

journal_title：Molecular and cellular biology

authors： Haracska L,Johnson RE,Prakash L,Prakash S

更新日期：2005-11-01 00:00:00

abstract：:SR proteins are essential for pre-mRNA splicing in vitro, act early in the splicing pathway, and can influence alternative splice site choice. Here we describe the isolation of both dominant and loss-of-function alleles of B52, the gene for a Drosophila SR protein. The allele B52ED was identified as a dominant second-...

journal_title：Molecular and cellular biology

authors： Peng X,Mount SM

更新日期：1995-11-01 00:00:00

abstract：:The carboxyl-terminal domain (CTD) of RNA polymerase II (Pol II) can be phosphorylated at serine 2 (Ser-2) and serine 5 (Ser-5) of the CTD heptad repeat YSPTSPS, and this phosphorylation is important in coupling transcription to RNA processing, including 5' capping, splicing, and polyadenylation. The mammalian endogen...

journal_title：Molecular and cellular biology

authors： Cheng C,Sharp PA

更新日期：2003-03-01 00:00:00

abstract：:An extracellular acid phosphatase was detected in the growth media of Leishmania donovani promastigotes. The enzyme was released at all stages of the growth cycle and in amounts which accounted for 90% of the total amount of this enzyme in the culture. The exoenzyme exhibited a pH optimum of 4.5 to 5.0 and was active ...

journal_title：Molecular and cellular biology

authors： Gottlieb M,Dwyer DM

更新日期：1982-01-01 00:00:00

abstract：:Insulin gene transcription relies on enhancer and promoter elements which are active in pancreatic beta cells. We showed that adenovirus type 5 infection of HIT T-15 cells, a transformed hamster beta cell line, represses insulin gene transcription and mRNA levels. Using expression plasmids transiently introduced into ...

journal_title：Molecular and cellular biology

authors： Stein RW,Ziff EB

更新日期：1987-03-01 00:00:00