Deciphering the cross talk between hnRNP K and c-Src: the c-Src activation domain in hnRNP K is distinct from a second interaction site.

abstract：:Streptozotocin (STZ) is widely used to induce diabetic rodent models. It is specifically toxic to pancreatic beta cells and causes severe destruction and dysfunction. We investigated the effect of 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG) on an STZ-induced diabetic mouse model. PLAG attenuated the glucose i...

journal_title：Molecular and cellular biology

authors： Kim J,Kim JH,Sohn KY,Yoon SY,Kim JW

更新日期：2019-10-11 00:00:00

abstract：:The murine lambda5-VpreB1 locus encodes two proteins that form part of the pre-B-cell receptor and play a key role in B-lymphocyte development. We have identified a locus control region (LCR) which is responsible for coordinate activation of both genes in pre-B cells. Analysis of mice with single and multiple copies o...

journal_title：Molecular and cellular biology

authors： Sabbattini P,Georgiou A,Sinclair C,Dillon N

更新日期：1999-01-01 00:00:00

abstract：:We find that immediately following transcript initiation, RNA polymerase II pauses at several locations even in the presence of relatively high (200 microM) levels of nucleoside triphosphates. Strong pauses with half-lives of >30 s were observed at +7, +18/19, and about +25 on the template used in these experiments. W...

journal_title：Molecular and cellular biology

authors： Pal M,Luse DS

更新日期：2002-01-01 00:00:00

abstract：:In Saccharomyces cerevisiae, COX5a and COX5b encode two distinct forms of cytochrome c oxidase subunit V, Va and Vb, respectively. To determine the relative contribution of COX5a and COX5b to cytochrome c oxidase function, we have disrupted each gene. Cytochrome c oxidase activity levels and respiration rates of strai...

journal_title：Molecular and cellular biology

authors： Trueblood CE,Poyton RO

更新日期：1987-10-01 00:00:00

abstract：:The ascomycete Saccharomyces cerevisiae exhibits alternative vegetative growth states referred to as the yeast form and the filamentous form, and it switches between the two morphologies depending on specific environmental signals. To identify molecules involved in control of morphologic differentiation, this study ch...

journal_title：Molecular and cellular biology

authors： Edgington NP,Blacketer MJ,Bierwagen TA,Myers AM

更新日期：1999-02-01 00:00:00

abstract：:A cell-free nuclear replication system that is S-phase specific, that requires the activity of DNA polymerase alpha, and that is stimulated three- to eightfold by cytoplasmic factors from S-phase cells was used to examine the temporal specificity of chromosomal DNA synthesis in vitro. Temporal specificity of DNA synth...

journal_title：Molecular and cellular biology

authors： Heintz NH,Stillman BW

更新日期：1988-05-01 00:00:00

abstract：:There is scant knowledge regarding how cell surface lipid-anchored T-cadherin (T-cad) transmits signals through the plasma membrane to its intracellular targets. This study aimed to identify membrane proteins colocalizing with atypical glycosylphosphatidylinositol (GPI)-anchored T-cad on the surface of endothelial cel...

journal_title：Molecular and cellular biology

authors： Philippova M,Ivanov D,Joshi MB,Kyriakakis E,Rupp K,Afonyushkin T,Bochkov V,Erne P,Resink TJ

更新日期：2008-06-01 00:00:00

abstract：:The chromatin structure of TDH3, one of three genes encoding glyceraldehyde phosphate dehydrogenases in Saccharomyces cerevisiae, was analyzed by nuclease digestion. A large hypersensitive region was found at the TDH3 promoter extending from the RNA initiation site at position -40 to position -560. This hypersensitive...

journal_title：Molecular and cellular biology

authors： Pavlović B,Hörz W

更新日期：1988-12-01 00:00:00

abstract：:Paramecium tetraurelia contains two types of nuclei, a diploid germinal micronucleus and a large transcriptionally active macronucleus. The macronuclear genome is formed from the micronuclear DNA during sexual reproduction. Previous studies have shown that the processing of the A-type variable surface protein gene dur...

journal_title：Molecular and cellular biology

authors： Scott JM,Mikami K,Leeck CL,Forney JD

更新日期：1994-04-01 00:00:00

abstract：:The mouse myosin light-chain 1A (MLC1A) gene, expressed in the atria of the adult heart, is one of the first muscle genes to be activated when skeletal as well as cardiac muscles form in the embryo. It is also transcribed in skeletal muscle cell lines at the onset of differentiation. Transient transfection assays of m...

journal_title：Molecular and cellular biology

authors： Catala F,Wanner R,Barton P,Cohen A,Wright W,Buckingham M

更新日期：1995-08-01 00:00:00

abstract：:Insulin treatment of Chinese hamster ovary (CHO) cells expressing high levels of the insulin receptor (CHO/IR cells) activates both c-fos serum response element and activator protein 1 (AP-1) reporter genes approximately 10-fold. In contrast, parental CHO cells display only two- to threefold insulin stimulation of rep...

journal_title：Molecular and cellular biology

authors： Yamauchi K,Pessin JE

更新日期：1994-07-01 00:00:00

abstract：:The large T antigen of simian virus 40 (SV40) is a multifunctional protein that is essential in both the virus lytic cycle and the oncogenic transformation of cells by SV40. To investigate the role of the numerous biochemical and physiological activities of T antigen in the lytic and transformation processes, we have ...

journal_title：Molecular and cellular biology

authors： Manos MM,Gluzman Y

更新日期：1984-06-01 00:00:00

abstract：:Costimulation of G protein-coupled receptors (GPCRs) may result in cross talk interactions between their downstream signaling pathways. Stimulation of GPCRs may also lead to cross talk regulation of receptor tyrosine kinase signaling and thereby to activation of mitogen-activated protein kinase (MAPK). In COS-7 cells,...

journal_title：Molecular and cellular biology

authors： Hanke S,Nürnberg B,Groll DH,Liebmann C

更新日期：2001-12-01 00:00:00

abstract：:Integration of protein kinases into transcription activation complexes influences the magnitude of gene expression. The nuclear factor of activated T cells (NFAT) group of proteins are critical transcription factors that direct gene expression in immune and nonimmune cells. A balance of phosphotransferase activity is ...

journal_title：Molecular and cellular biology

authors： Yang TT,Xiong Q,Graef IA,Crabtree GR,Chow CW

更新日期：2005-02-01 00:00:00

abstract：:We have developed a high-frequency cotransformation system which is useful in introducing nonreplicating circular DNA plasmids into the fission yeast Schizosaccharomyces pombe. This system depends on two factors: the ability of the ural-complementing helper plasmids pFYM2 and pFYM225 to propagate autonomously in S. po...

journal_title：Molecular and cellular biology

authors： Sakai K,Sakaguchi J,Yamamoto M

更新日期：1984-04-01 00:00:00

abstract：:The levels of intramolecular plasmid recombination, following transfection of a plasmid substrate for homologous recombination into normal and immortally transformed cells, have been examined by two independent assays. In the first assay, recovered plasmid was tested for DNA rearrangements which regenerate a functiona...

journal_title：Molecular and cellular biology

authors： Finn GK,Kurz BW,Cheng RZ,Shmookler Reis RJ

更新日期：1989-09-01 00:00:00

abstract：:Interferon A (IFN-A) genes are differentially expressed after virus induction. The differential expression of individual IFN-A genes is modulated by the specific transcription activators IFN regulatory factor 3 (IRF3) and IRF-7 and the homeoprotein transcription repressor Pitx1. We now show that repression by Pitx1 do...

journal_title：Molecular and cellular biology

authors： Island ML,Mesplede T,Darracq N,Bandu MT,Christeff N,Djian P,Drouin J,Navarro S

更新日期：2002-10-01 00:00:00

abstract：:The metabolic lifetime of mRNA can be specified by specific cis-acting elements within mRNA. One type of element is an adenylate- and uridylate-rich element (ARE) found in the 3' untranslated region of many highly unstable mRNAs for mammalian early-response genes (ERGs). Among the better-characterized members of the E...

journal_title：Molecular and cellular biology

authors： Chen CY,Shyu AB

更新日期：1994-12-01 00:00:00

abstract：:The small subunit of ribonucleotide reductase in Saccharomyces cerevisiae (RNR2) was induced 3- to 20-fold by a variety of DNA-damaging agents. Induction of the RNR2 transcript by at least one of these agents, methyl methanesulfonate, did not require protein synthesis. To identify sequences involved in the regulation ...

journal_title：Molecular and cellular biology

authors： Hurd HK,Roberts JW

更新日期：1989-12-01 00:00:00

abstract：:The translational GTPases promote initiation, elongation, and termination of protein synthesis by interacting with the ribosome. Mutations that impair GTP hydrolysis by eukaryotic translation initiation factor 5B/initiation factor 2 (eIF5B/IF2) impair yeast cell growth due to failure to dissociate from the ribosome fo...

journal_title：Molecular and cellular biology

authors： Shin BS,Kim JR,Acker MG,Maher KN,Lorsch JR,Dever TE

更新日期：2009-02-01 00:00:00

abstract：:We have functionally tested derivatives of a muscle-specific Drosophila melanogaster actin gene in which 5'-flanking sequences have been deleted or rearranged. From our results we conclude that approximately 1,000 nucleotides of 5'-flanking sequence are required for wild-type levels of mRNA accumulation during flight ...

journal_title：Molecular and cellular biology

authors： Geyer PK,Fyrberg EA

更新日期：1986-10-01 00:00:00

abstract：:The location in the mouse genome of the 149-base pair MES-1 element, previously isolated by its ability to restore expression to an enhancerless selectable gene, was analyzed. The active moiety of the single-copy MES-1 element is located between the 5' ends of two divergent transcription units, SURF-1 and SURF-2, both...

journal_title：Molecular and cellular biology

authors： Williams TJ,Fried M

更新日期：1986-12-01 00:00:00

abstract：:CD83 is one of the best-known surface markers for fully mature dendritic cells (mature DCs), and its cell-type- and maturation-specific regulation makes the CD83 promoter an interesting tool for the genetic modulation of DCs. To determine the mechanisms regulating this DC- and maturation-specific CD83 expression, chro...

journal_title：Molecular and cellular biology

authors： Stein MF,Lang S,Winkler TH,Deinzer A,Erber S,Nettelbeck DM,Naschberger E,Jochmann R,Stürzl M,Slany RK,Werner T,Steinkasserer A,Knippertz I

更新日期：2013-04-01 00:00:00

abstract：:Phospholipase Cgamma2 (PLCgamma2) is critical for pre-B-cell receptor (pre-BCR) and BCR signaling. Current studies discovered that PLCgamma2-deficient mice had reduced immunoglobulin lambda (Iglambda) light-chain usage throughout B-cell maturation stages, including transitional type 1 (T1), transitional type 2 (T2), a...

journal_title：Molecular and cellular biology

authors： Bai L,Chen Y,He Y,Dai X,Lin X,Wen R,Wang D

更新日期：2007-09-01 00:00:00

abstract：:Angiotensin II (AII), a potent vasoactive hormone, acts on numerous organs via G-protein-coupled receptors and elicits cell-specific responses. At the level of the heart, AII stimulation alters gene transcription and leads to cardiomyocyte hypertrophy. Numerous intracellular signaling pathways are activated in this pr...

journal_title：Molecular and cellular biology

authors： Wang J,Paradis P,Aries A,Komati H,Lefebvre C,Wang H,Nemer M

更新日期：2005-11-01 00:00:00

abstract：:Nuclear pore complexes (NPCs) play an essential role in RNA export. Nucleoporins required for mRNA export in Saccharomyces cerevisiae are found in the Nup84p and Nup82p subcomplexes of the NPC. The Nup82p subcomplex contains Nup82p, Rat7p/Nup159p, Nsp1p, Gle1p/Rss1p, and Rip1p/Nup42p and is found only on the cytoplasm...

journal_title：Molecular and cellular biology

authors： Rollenhagen C,Hodge CA,Cole CN

更新日期：2004-06-01 00:00:00

abstract：:Histone methylation is an important posttranslational modification that contributes to chromatin-based processes including transcriptional regulation, DNA repair, and epigenetic inheritance. In the budding yeast Saccharomyces cerevisiae, histone lysine methylation occurs on histone H3 lysines 4, 36, and 79, and its de...

journal_title：Molecular and cellular biology

authors： Klose RJ,Gardner KE,Liang G,Erdjument-Bromage H,Tempst P,Zhang Y

更新日期：2007-06-01 00:00:00

abstract：:The Suppressor of the Hairy wing [Su(Hw)] binding region within the gypsy retrotransposon is the best known chromatin insulator in Drosophila melanogaster. According to previous data, two copies of the gypsy insulator inserted between an enhancer and a promoter neutralize each other's actions, which is indicative of a...

journal_title：Molecular and cellular biology

authors： Kravchenko E,Savitskaya E,Kravchuk O,Parshikov A,Georgiev P,Savitsky M

更新日期：2005-11-01 00:00:00

abstract：:Sequencing studies have shown that in somatic cells alternating runs of purines and pyrimidines are frequently associated with recombination crossover points. To test whether such sequences actually promote recombination, we have examined the effects of poly[d(pGpT).d(pApC)] and poly[d(pCpG).d(pCpG)] repeats on a homo...

journal_title：Molecular and cellular biology

authors： Bullock P,Miller J,Botchan M

更新日期：1986-11-01 00:00:00

abstract：:Hypoxia induces apoptosis but also triggers adaptive mechanisms to ensure cell survival. Here we show that the prosurvival effects of hypoxia-inducible factor 1 (HIF-1) in endothelial cells are mediated by neuron-derived orphan receptor 1 (NOR-1). The overexpression of NOR-1 decreased the rate of endothelial cells und...

journal_title：Molecular and cellular biology

authors： Martorell L,Gentile M,Rius J,Rodríguez C,Crespo J,Badimon L,Martínez-González J

更新日期：2009-11-01 00:00:00