Abstract:
:The small subunit of ribonucleotide reductase in Saccharomyces cerevisiae (RNR2) was induced 3- to 20-fold by a variety of DNA-damaging agents. Induction of the RNR2 transcript by at least one of these agents, methyl methanesulfonate, did not require protein synthesis. To identify sequences involved in the regulation of RNR2, we introduced deletions upstream of the transcription start site. Sequences required for induction were contained within a 200-base-pair region that could confer methyl methanesulfonate inducibility on the heterologous CYC1 promoter. This region contained a repression sequence and at least two positive activation sites. One of these activation sites bound RAP1, a protein known to associate with mating-type silencers and the upstream activation sequences of a number of genes. The behavior of deletions of the repression sequence suggests that induction of RNR2 may occur, at least in part, through relief of repression.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Hurd HK,Roberts JWdoi
10.1128/mcb.9.12.5359subject
Has Abstractpub_date
1989-12-01 00:00:00pages
5359-72issue
12eissn
0270-7306issn
1098-5549journal_volume
9pub_type
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.10.9.4650
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/mcb.18.2.742
更新日期:1998-02-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.9.8.3292
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pub_type: 杂志文章
doi:10.1128/mcb.19.10.6710
更新日期:1999-10-01 00:00:00
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更新日期:1999-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/MCB.06077-11
更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.23.17.6187-6199.2003
更新日期:2003-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1988-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-11-01 00:00:00