Abstract:
:Using a hybridoma cell line which secretes hapten-specific immunoglobulin M (IgM), we have isolated a variety of mutants which produce abnormal immunoglobulin. Immunoglobulin was tested for the size and composition of the component heavy and light chains and for variable and constant region related functional and serological activities. Some mutants secrete IgM which seems to be defective in hapten binding; others make IgM which appears not to activate complement. Many of the mutants secrete monomeric as opposed to pentameric IgM. In some cases, the defect apparently correlates with structural alterations in the mu heavy chain: partial deletion, polypeptide addition, and abnormal glycosylation have been observed. These mutant cell lines provide a means of identifying the structural basis of IgM function and of studying the biochemistry of IgM synthesis and processing.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Shulman MJ,Heusser C,Filkin C,Köhler Gdoi
10.1128/mcb.2.9.1033subject
Has Abstractpub_date
1982-09-01 00:00:00pages
1033-43issue
9eissn
0270-7306issn
1098-5549journal_volume
2pub_type
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