Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.

abstract：:The six human telomeric proteins TRF1, TRF2, RAP1, TIN2, POT1, and TPP1 can form a complex called the telosome/shelterin, which is required for telomere protection and length control. TPP1 has been shown to regulate both POT1 telomere localization and telosome assembly through its binding to TIN2. It remains to be det...

journal_title：Molecular and cellular biology

authors： Chen LY,Liu D,Songyang Z

更新日期：2007-08-01 00:00:00

abstract：:To understand how DEXD/H-box proteins recognize and interact with their cellular substrates, we have been studying Prp28p, a DEXD/H-box splicing factor required for switching the U1 snRNP with the U6 snRNP at the precursor mRNA (pre-mRNA) 5' splice site. We previously demonstrated that the requirement for Prp28p can b...

journal_title：Molecular and cellular biology

authors： Hage R,Tung L,Du H,Stands L,Rosbash M,Chang TH

更新日期：2009-07-01 00:00:00

abstract：:We have investigated the in vivo and in vitro regulation of the human urokinase-type plasminogen activator (uPA) gene by interleukin-1 (IL-1) and analyzed the transcription factors and signalling pathways involved in the response of the -2.0-kb uPA enhancer to IL-1 induction and to tetradecanoyl phorbol acetate (TPA) ...

journal_title：Molecular and cellular biology

authors： Cirillo G,Casalino L,Vallone D,Caracciolo A,De Cesare D,Verde P

更新日期：1999-09-01 00:00:00

abstract：:The conserved RNA binding protein La recognizes UUU-3'OH on its small nuclear RNA ligands and stabilizes them against 3'-end-mediated decay. We report that newly described La-related protein 4 (LARP4) is a factor that can bind poly(A) RNA and interact with poly(A) binding protein (PABP). Yeast two-hybrid analysis and ...

journal_title：Molecular and cellular biology

authors： Yang R,Gaidamakov SA,Xie J,Lee J,Martino L,Kozlov G,Crawford AK,Russo AN,Conte MR,Gehring K,Maraia RJ

更新日期：2011-02-01 00:00:00

abstract：:Cytoplasmic poly(A) elongation is one mechanism that regulates translational recruitment of maternal mRNA in early development. In Xenopus laevis, poly(A) elongation is controlled by two cis elements in the 3' untranslated regions of responsive mRNAs: the hexanucleotide AAUAAA and a U-rich structure with the general s...

journal_title：Molecular and cellular biology

authors： Stebbins-Boaz B,Richter JD

更新日期：1994-09-01 00:00:00

abstract：:We provide evidence that the human DNA ligase III gene encodes a mitochondrial form of this enzyme. First, the DNA ligase III cDNA contains an in-frame ATG located upstream from the putative translation initiation start site. The DNA sequence between these two ATG sites encodes an amphipathic helix similar to previous...

journal_title：Molecular and cellular biology

authors： Lakshmipathy U,Campbell C

更新日期：1999-05-01 00:00:00

abstract：:Tie1 is an orphan receptor tyrosine kinase that is expressed almost exclusively in endothelial cells and that is required for normal embryonic vascular development. Genetic studies suggest that Tie1 promotes endothelial cell survival, but other studies have suggested that the Tie1 kinase has little to no activity, and...

journal_title：Molecular and cellular biology

authors： Kontos CD,Cha EH,York JD,Peters KG

更新日期：2002-03-01 00:00:00

abstract：:A variety of genes, including c-myc, are activated by chemical toxicants in vivo and in vitro. Although enforced c-myc expression induces apoptosis after withdrawing survival factors, it is not clear if activation of the endogenous c-myc gene is an apoptotic signal after toxicant exposure. The renal tubular epithelium...

journal_title：Molecular and cellular biology

authors： Zhan Y,Cleveland JL,Stevens JL

更新日期：1997-11-01 00:00:00

abstract：:Swi4 and Swi6 form a complex which is required for Start-dependent activation of HO and for high-level expression of G1 cyclin genes CLN1 and CLN2. To identify other regulators of this pathway, we screened for dominant, recessive, conditional, and allele-specific suppressors of swi4 mutants. We isolated 16 recessive s...

journal_title：Molecular and cellular biology

authors： Lycan D,Mikesell G,Bunger M,Breeden L

更新日期：1994-11-01 00:00:00

abstract：:Using chromatin immunoprecipitation assays, we studied the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-mediated recruitment of the aryl hydrocarbon receptor (AhR) and several co-regulators to the CYP1A1 promoter. AhR displayed a time-dependent recruitment, reaching a peak at 75 min and maintaining promoter occupancy fo...

journal_title：Molecular and cellular biology

authors： Matthews J,Wihlén B,Thomsen J,Gustafsson JA

更新日期：2005-07-01 00:00:00

abstract：:We have investigated mitochondrial import and processing of the precursor for human ornithine transcarbamylase (OTC; carbamoylphosphate:L-ornithine carbamoyltransferase, EC 2.1.3.3) in HeLa cells stably transformed with cDNA sequences encoding OTC precursors carrying mutations in their leader peptides. The mutant prec...

journal_title：Molecular and cellular biology

authors： Isaya G,Fenton WA,Hendrick JP,Furtak K,Kalousek F,Rosenberg LE

更新日期：1988-12-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) Tat protein activates transcription elongation by stimulating the Tat-activated kinase (TAK/p-TEFb), a protein kinase composed of CDK9 and its cyclin partner, cyclin T1. CDK9 is able to hyperphosphorylate the carboxyl-terminal domain (CTD) of the large subunit of RNA pol...

journal_title：Molecular and cellular biology

authors： Bourgeois CF,Kim YK,Churcher MJ,West MJ,Karn J

更新日期：2002-02-01 00:00:00

abstract：:Genomic imprinting results in parent-of-origin-dependent monoallelic expression of selected genes. Although their importance in development and physiology is recognized, few imprinted genes have been investigated for their effects on brain function. Gnas is a complex imprinted locus whose gene products are involved in...

journal_title：Molecular and cellular biology

authors： Plagge A,Isles AR,Gordon E,Humby T,Dean W,Gritsch S,Fischer-Colbrie R,Wilkinson LS,Kelsey G

更新日期：2005-04-01 00:00:00

abstract：:The human genome contains a number of methyl CpG binding proteins that translate DNA methylation into a physiological response. To gain insight into the function of MBD2 and MBD3, we first applied protein tagging and mass spectrometry. We show that MBD2 and MBD3 assemble into mutually exclusive distinct Mi-2/NuRD-like...

journal_title：Molecular and cellular biology

authors： Le Guezennec X,Vermeulen M,Brinkman AB,Hoeijmakers WA,Cohen A,Lasonder E,Stunnenberg HG

更新日期：2006-02-01 00:00:00

abstract：:Cell polarity plays a critical role in the development of all metazoans; however, the mechanisms of cell polarity and the specific role of cell polarity pathways in mammalian organisms are still poorly understood. Lethal giant larvae (Lgl) is an apical-basal polarity gene identified in Drosophila, where it functions a...

journal_title：Molecular and cellular biology

authors： Sripathy S,Lee M,Vasioukhin V

更新日期：2011-07-01 00:00:00

abstract：:The epidermal growth factor (EGF) receptor is the functional target of the mitogen EGF and the cellular homolog of the avian erythroblastosis virus erbB oncogene product. Regulation of expression of the proto-oncogene encoding the EGF receptor can be elucidated by studying the structure and function of the gene promot...

journal_title：Molecular and cellular biology

authors： Johnson AC,Jinno Y,Merlino GT

更新日期：1988-10-01 00:00:00

abstract：:A variant polyadenylation signal, which is conserved and employed by mammalian hepadnaviruses, has a sequence resembling that of the TATA box. We report here that this composite box manifests all the promoter characteristics. It binds effectively TATA-binding protein with TFIIB and TFIIA in a synergistic manner. This ...

journal_title：Molecular and cellular biology

authors： Paran N,Ori A,Haviv I,Shaul Y

更新日期：2000-02-01 00:00:00

abstract：:Gradients of Wnt/beta-catenin signaling coordinate development and physiological homeostasis in metazoan animals. Proper embryonic development of the fruit fly Drosophila melanogaster requires the Naked cuticle (Nkd) protein to attenuate a gradient of Wnt/beta-catenin signaling across each segmental anlage. Nkd inhibi...

journal_title：Molecular and cellular biology

authors： Zhang S,Cagatay T,Amanai M,Zhang M,Kline J,Castrillon DH,Ashfaq R,Oz OK,Wharton KA Jr

更新日期：2007-06-01 00:00:00

abstract：:Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor Oct3/4 is an indispensable factor in the self-renewal of ES cells. In this study, we searched for a protein that would interact with Oct3/4 in ES cells and identified an orphan nuclear hormone receptor...

journal_title：Molecular and cellular biology

authors： Sun C,Nakatake Y,Akagi T,Ura H,Matsuda T,Nishiyama A,Koide H,Ko MS,Niwa H,Yokota T

更新日期：2009-08-01 00:00:00

abstract：:TIN2 is an important regulator of telomere length, and mutations in TINF2, the gene encoding TIN2, cause short-telomere syndromes. While the genetics underscore the importance of TIN2, the mechanism through which TIN2 regulates telomere length remains unclear. Here, we tested the effects of human TIN2 on telomerase ac...

journal_title：Molecular and cellular biology

authors： Pike AM,Strong MA,Ouyang JPT,Greider CW

更新日期：2019-10-11 00:00:00

abstract：:The RPC31 gene encoding the C31 subunit of Saccharomyces cerevisiae RNA polymerase C (III) has been isolated, starting from a C-terminal fragment cloned on a lambda gt11 library. It is unique on the yeast genome and lies on the left arm of chromosome XIV, very close to a NotI site. Its coding sequence perfectly matche...

journal_title：Molecular and cellular biology

authors： Mosrin C,Riva M,Beltrame M,Cassar E,Sentenac A,Thuriaux P

更新日期：1990-09-01 00:00:00

abstract：:We have identified a complex between TFIIIB and the upstream promoter of silkworm tRNA Ala genes that is detectable by gel retardation and DNase I footprinting. Formation of this complex depends on the integrity of previously identified upstream promoter elements and on the presence of other silkworm transcription fac...

journal_title：Molecular and cellular biology

authors： Young LS,Ahnert N,Sprague KU

更新日期：1996-03-01 00:00:00

abstract：:Previous studies indicated that repression by eve involves cooperative DNA binding and leads to the formation of a DNA loop which encompasses the DNA sequences normally bound by the RNA polymerase II general transcription factors. To test the general principle of whether bending of a basal promoter sequence can contri...

journal_title：Molecular and cellular biology

authors： TenHarmsel A,Biggin MD

更新日期：1995-10-01 00:00:00

abstract：:EGL-15 is a fibroblast growth factor receptor in the nematode Caenorhabditis elegans. Components that mediate EGL-15 signaling have been identified via mutations that confer a Clear (Clr) phenotype, indicative of hyperactivity of this pathway, or a suppressor-of-Clr (Soc) phenotype, indicative of reduced pathway activ...

journal_title：Molecular and cellular biology

authors： Schutzman JL,Borland CZ,Newman JC,Robinson MK,Kokel M,Stern MJ

更新日期：2001-12-01 00:00:00

abstract：:Cytosine methylation at CpG dinucleotides is thought to cause more than one-third of all transition mutations responsible for human genetic diseases and cancer. We investigated the methylation status of the CpG dinucleotide at codon 248 in exon 7 of the p53 gene because this codon is a hot spot for inactivating mutati...

journal_title：Molecular and cellular biology

authors： Magewu AN,Jones PA

更新日期：1994-06-01 00:00:00

abstract：:The nuclear vitamin D receptor (VDR) is a member of a nuclear receptor superfamily and acts as a ligand-dependent transcription factor. A family of cotranscriptional activators (SRC-1, TIF2, and AIB-1) interacts with and activates the transactivation function of nuclear receptors in a ligand-dependent way. We examined...

journal_title：Molecular and cellular biology

authors： Takeyama K,Masuhiro Y,Fuse H,Endoh H,Murayama A,Kitanaka S,Suzawa M,Yanagisawa J,Kato S

更新日期：1999-02-01 00:00:00

abstract：:A prominent hallmark of most human cancer is aneuploidy, which is a result of the chromosomal instability of cancer cells and is thought to contribute to the initiation and progression of most carcinomas. The developmentally regulated GATA6 transcription factor is commonly lost in ovarian cancer, and the loss of its e...

journal_title：Molecular and cellular biology

authors： Capo-chichi CD,Cai KQ,Testa JR,Godwin AK,Xu XX

更新日期：2009-09-01 00:00:00

abstract：:Cells respond to environmental stimuli by fine-tuned regulation of gene expression. Here we investigated the dose-dependent modulation of gene expression at high temporal resolution in response to nutrient and stress signals in yeast. The GAL1 activity in cell populations is modulated in a well-defined range of galact...

journal_title：Molecular and cellular biology

authors： Rienzo A,Poveda-Huertes D,Aydin S,Buchler NE,Pascual-Ahuir A,Proft M

更新日期：2015-11-01 00:00:00

abstract：:Previous studies have shown that exposure of cells to high levels of replicational stress leads to permanent proliferation arrest that does not require p53. We have examined cellular responses to therapeutically relevant low levels of replicational stress that allow limited proliferation. Chronic exposure to low conce...

journal_title：Molecular and cellular biology

authors： Marusyk A,Wheeler LJ,Mathews CK,DeGregori J

更新日期：2007-08-01 00:00:00

abstract：:Transfection of a hamster cell mutant with human DNA corrected both the low-density lipoprotein receptor-deficient phenotype and the multiple glycosylation defects of the cells. Independently transfected colonies contained a small set of common human DNA fragments. These fragments may correspond to the human analog of...

journal_title：Molecular and cellular biology

authors： Kingsley DM,Sege RD,Kozarsky KF,Krieger M

更新日期：1986-07-01 00:00:00