DNA-mediated transfer of a human gene required for low-density lipoprotein receptor expression and for multiple Golgi processing pathways.

abstract：:To characterize further the regulatory mechanism modulating the expression of the Saccharomyces cerevisiae ARG3 gene, i.e., the specific repression by arginine and the general amino acid control, we analyzed by deletion the region upstream of that gene, determined the nucleotide sequence of operator-constitutive-like ...

journal_title：Molecular and cellular biology

authors： Crabeel M,Huygen R,Verschueren K,Messenguy F,Tinel K,Cunin R,Glansdorff N

更新日期：1985-11-01 00:00:00

abstract：:The GLN3 gene of Saccharomyces cerevisiae is required for the activation of transcription of a number of genes in response to the replacement of glutamine by glutamate as source of nitrogen. We cloned the GLN3 gene and constructed null alleles by gene disruption. GLN3 is not essential for growth, but increased copies ...

journal_title：Molecular and cellular biology

authors： Minehart PL,Magasanik B

更新日期：1991-12-01 00:00:00

abstract：:A synthetic DNA probe designed to detect coding sequences for platelet factor 4 and connective tissue-activating peptide III (two human platelet alpha-granule proteins) was used to identify several similar sequences in total human DNA. Sequence analysis of a corresponding 3,201-base-pair EcoRI fragment isolated from a...

journal_title：Molecular and cellular biology

authors： Green CJ,Charles RS,Edwards BF,Johnson PH

更新日期：1989-04-01 00:00:00

abstract：:The Neurospora crassa arg-2 upstream open reading frame (uORF) plays a role in negative arginine-specific translational regulation. Primer extension inhibition analyses of arg-2 uORF-containing RNA translated in a cell-free system in which arginine-specific regulation was retained revealed "toeprints" corresponding to...

journal_title：Molecular and cellular biology

authors： Wang Z,Sachs MS

更新日期：1997-09-01 00:00:00

abstract：:Amino acids 106 to 143 and 354 to 433 of the human c-myc protein (439 amino acids) were shown to be required for the protein to suppress c-myc gene transcription and were found to exactly overlap with those necessary for c-myc to cooperate with ras oncogenes in the transformation of rat embryo fibroblasts. The essenti...

journal_title：Molecular and cellular biology

authors： Penn LJ,Brooks MW,Laufer EM,Littlewood TD,Morgenstern JP,Evan GI,Lee WM,Land H

更新日期：1990-09-01 00:00:00

abstract：:The full-length versions of the Ras-specific exchange factors Ras-GRF1 (GRF1) and Ras-GRF2 (GRF2), which are expressed in brain and a restricted number of other organs, possess an ionomycin-dependent activation of Erk mitogen-activated protein kinase activity in 293T cells (C. L. Farnsworth et al., Nature 376:524-527,...

journal_title：Molecular and cellular biology

authors： Anborgh PH,Qian X,Papageorge AG,Vass WC,DeClue JE,Lowy DR

更新日期：1999-07-01 00:00:00

abstract：:Imprinted genes are expressed in a monoallelic, parent-of-origin-specific manner. Clusters of imprinted genes are regulated by imprinting control regions (ICRs) characterized by DNA methylation of one allele. This methylation is critical for imprinting; a reduction in the DNA methyltransferase DNMT1 causes a widesprea...

journal_title：Molecular and cellular biology

authors： Weaver JR,Sarkisian G,Krapp C,Mager J,Mann MR,Bartolomei MS

更新日期：2010-08-01 00:00:00

abstract：:We recently identified Vav, the product of the vav proto-oncogene, as a guanine nucleotide exchange factor (GEF) for Ras. Vav is enzymatically activated by lymphocyte antigen receptor-coupled protein tyrosine kinases or independently by diglycerides. To further evaluate the physiological role of Vav, we assessed its G...

journal_title：Molecular and cellular biology

authors： Gulbins E,Coggeshall KM,Langlet C,Baier G,Bonnefoy-Berard N,Burn P,Wittinghofer A,Katzav S,Altman A

更新日期：1994-02-01 00:00:00

abstract：:It has previously been demonstrated that the simian virus 40 large T antigen and adenovirus E1A proteins can form complexes with the retinoblastoma susceptibility gene product (RB). We studied the ability of these proteins to bind to mutant RB proteins in vitro. A region of RB spanning residues 379 to 792 was found to...

journal_title：Molecular and cellular biology

authors： Kaelin WG Jr,Ewen ME,Livingston DM

更新日期：1990-07-01 00:00:00

abstract：:Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in phosphoinositide turnover and is involved in a variety of physiological functions. We analyzed PLCdelta1 knockout mice and found that PLCdelta1 is required for the maintenance of skin homeostasis. However, there were no remarkable abnormalities except ...

journal_title：Molecular and cellular biology

authors： Nakamura Y,Hamada Y,Fujiwara T,Enomoto H,Hiroe T,Tanaka S,Nose M,Nakahara M,Yoshida N,Takenawa T,Fukami K

更新日期：2005-12-01 00:00:00

abstract：:RAD52 and RAD9 are required for the repair of double-strand breaks (DSBs) induced by physical and chemical DNA-damaging agents in Saccharomyces cerevisiae. Analysis of EcoRI endonuclease expression in vivo revealed that, in contrast to DSBs containing damaged or modified termini, chromosomal DSBs retaining complementa...

journal_title：Molecular and cellular biology

authors： Lewis LK,Kirchner JM,Resnick MA

更新日期：1998-04-01 00:00:00

abstract：:The locus control region (LCR) regulates transcription of the downstream beta-like globin genes 10 to 50 kb away. Among hypersensitive sites HS4, -3, -2, and -1, which define the LCR in erythroid cells, HS2 possesses prominent enhancer function. The mechanism by which the HS2 enhancer and other functional components o...

journal_title：Molecular and cellular biology

authors： Kong S,Bohl D,Li C,Tuan D

更新日期：1997-07-01 00:00:00

abstract：:The autonomously replicating rRNA genes (rDNA) in the somatic nucleus of Tetrahymena thermophila are maintained at a copy number of approximately 10(4) per nucleus. A mutant in which the replication properties of this molecule were altered was isolated and characterized. This mutation of inbred strain C3, named rmm4, ...

journal_title：Molecular and cellular biology

authors： Yaeger PC,Orias E,Shaiu WL,Larson DD,Blackburn EH

更新日期：1989-02-01 00:00:00

abstract：:The HRX gene (also called MLL, ALL-1, and Htrx) at chromosome band 11q23 is associated with specific subsets of acute leukemias through translocations that result in its fusion with a variety of heterologous partners. Two of these partners, ENL and AF9, code for proteins that are highly similar to each other and as fu...

journal_title：Molecular and cellular biology

authors： Slany RK,Lavau C,Cleary ML

更新日期：1998-01-01 00:00:00

abstract：:IMP dehydrogenase (IMPDH) is the rate-limiting enzyme in the de novo synthesis of guanine nucleotides. It is a target of therapeutically useful drugs and is implicated in the regulation of cell growth rate. In the yeast Saccharomyces cerevisiae, mutations in components of the RNA polymerase II (Pol II) transcription e...

journal_title：Molecular and cellular biology

authors： Shaw RJ,Reines D

更新日期：2000-10-01 00:00:00

abstract：:p21Sdi1/WAF1/Cip1 inhibits cyclin-dependent protein kinases and cell proliferation. p21 is presumed to inhibit growth by preventing the phosphorylation of growth-regulatory proteins, including the retinoblastoma tumor suppressor protein (pRb). The ultimate effector(s) of p21 growth inhibition, however, is largely a ma...

journal_title：Molecular and cellular biology

authors： Dimri GP,Nakanishi M,Desprez PY,Smith JR,Campisi J

更新日期：1996-06-01 00:00:00

abstract：:2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other aryl hydrocarbon receptor (AhR) ligands suppress 17beta-estradiol (E)-induced responses in the rodent uterus and mammary tumors and in human breast cancer cells. Treatment of ZR-75, T47D, and MCF-7 human breast cancer cells with TCDD induces proteasome-dependent deg...

journal_title：Molecular and cellular biology

authors： Wormke M,Stoner M,Saville B,Walker K,Abdelrahim M,Burghardt R,Safe S

更新日期：2003-03-01 00:00:00

abstract：:Neuronal differentiation is tightly regulated by a variety of factors. In a search for neuron-specific genes, we identified a highly conserved novel zinc finger protein, ZNF536. We observed that ZNF536 is most abundant in the brain and, in particular, is expressed in the developing central nervous system and dorsal ro...

journal_title：Molecular and cellular biology

authors： Qin Z,Ren F,Xu X,Ren Y,Li H,Wang Y,Zhai Y,Chang Z

更新日期：2009-07-01 00:00:00

abstract：:Progesterone receptor (PR) and ErbB-2 bidirectional cross talk participates in breast cancer development. Here, we identified a new mechanism of the PR and ErbB-2 interaction involving the PR induction of ErbB-2 nuclear translocation and the assembly of a transcriptional complex in which ErbB-2 acts as a coactivator o...

journal_title：Molecular and cellular biology

authors： Béguelin W,Díaz Flaqué MC,Proietti CJ,Cayrol F,Rivas MA,Tkach M,Rosemblit C,Tocci JM,Charreau EH,Schillaci R,Elizalde PV

更新日期：2010-12-01 00:00:00

abstract：:Genetic and biochemical experiments have enabled us to more clearly distinguish three genetic loci, emtA, emtB, and emtC, all of which can be altered to give rise to resistance to the protein synthesis inhibitor, emetine, in cultured Chinese hamster cells. Genetic experiments have demonstrated that, unlike the emtB lo...

journal_title：Molecular and cellular biology

authors： Chang S,Wasmuth JJ

更新日期：1983-03-01 00:00:00

abstract：:Splicing of tRNA precursors in Saccharomyces cerevisiae extracts proceeds in two steps; excision of the intervening sequence and ligation of the tRNA halves. The ability to resolve these two steps and the distinct physical properties of the endonuclease and ligase suggested that the splicing steps may not be concerted...

journal_title：Molecular and cellular biology

authors： Greer CL

更新日期：1986-02-01 00:00:00

abstract：:Two alpha-tubulin genes from the budding yeast Saccharomyces cerevisiae were identified and cloned by cross-species DNA homology. Nucleotide sequencing studies revealed that the two genes, named TUB1 and TUB3, encoded gene products of 447 and 445 amino acids, respectively, that are highly homologous to alpha-tubulins ...

journal_title：Molecular and cellular biology

authors： Schatz PJ,Pillus L,Grisafi P,Solomon F,Botstein D

更新日期：1986-11-01 00:00:00

abstract：:The kidney's vital filtration function depends on the structural integrity of the glomerulus, the proximal portion of the nephron. Within the glomerulus, the architecturally complex podocyte forms the final cellular barrier to filtration. Injury to the podocyte results in a morphological change called foot process eff...

journal_title：Molecular and cellular biology

authors： Yu H,Suleiman H,Kim AH,Miner JH,Dani A,Shaw AS,Akilesh S

更新日期：2013-12-01 00:00:00

abstract：:The genes of the trithorax group (trxG) in Drosophila melanogaster are required to maintain the pattern of homeotic gene expression that is established early in embryogenesis by the transient expression of the segmentation genes. The precise role of each of the diverse trxG members and the functional relationships amo...

journal_title：Molecular and cellular biology

authors： Crosby MA,Miller C,Alon T,Watson KL,Verrijzer CP,Goldman-Levi R,Zak NB

更新日期：1999-02-01 00:00:00

abstract：:We have screened an adult rat cerebellar cDNA library in search of novel protein tyrosine-kinase (PTK) cDNAs. A cDNA for a putative PTK, trkB, was cloned, and its sequence indicates that it is likely to be derived from a gene for a ligand-regulated receptor closely related to the human trk oncogene. Northern (RNA) ana...

journal_title：Molecular and cellular biology

authors： Middlemas DS,Lindberg RA,Hunter T

更新日期：1991-01-01 00:00:00

abstract：:Centrins in vertebrates have traditionally been associated with microtubule-nucleating centers such as the centrosome. Unexpectedly, we found centrin 2 to associate biochemically with nucleoporins, including the Xenopus laevis Nup107-160 complex, a critical subunit of the vertebrate nuclear pore in interphase and of t...

journal_title：Molecular and cellular biology

authors： Resendes KK,Rasala BA,Forbes DJ

更新日期：2008-03-01 00:00:00

abstract：:Thrombospondins are a family of stress-inducible secreted glycoproteins that underlie tissue remodeling. We recently reported that thrombospondin-4 (Thbs4) has a critical intracellular function, regulating the adaptive endoplasmic reticulum (ER) stress pathway through activating transcription factor 6α (Atf6α). In the...

journal_title：Molecular and cellular biology

authors： Brody MJ,Schips TG,Vanhoutte D,Kanisicak O,Karch J,Maliken BD,Blair NS,Sargent MA,Prasad V,Molkentin JD

更新日期：2015-10-12 00:00:00

abstract：:The eukaryotic genome is divided into chromosomal domains of distinct gene activities. Transcriptionally silent chromatin tends to encroach upon active chromatin. Barrier elements that can block the spread of silent chromatin have been documented, but the mechanisms of their function are not resolved. We show that the...

journal_title：Molecular and cellular biology

authors： Bi X,Yu Q,Sandmeier JJ,Zou Y

更新日期：2004-03-01 00:00:00

abstract：:Chicken heart mesenchymal cells do not proliferate in medium of physiological composition containing plasma (S. Balk, Proc. Natl. Acad. Sci. USA 77:6606-6610, 1980). To understand the molecular events involved in cell quiescence and in the initiation of cell division under physiological conditions, we examined the dif...

journal_title：Molecular and cellular biology

authors： Bédard PA,Balk SD,Gunther HS,Morisi A,Erikson RL

更新日期：1987-04-01 00:00:00

abstract：:Activins and other members of the transforming growth factor beta family play a critical role in morphological changes of the epidermis that require epithelial cell movement. We investigated the molecular pathways in the transmission of activin signals that lead to actin reorganization and epithelial cell migration. W...

journal_title：Molecular and cellular biology

authors： Zhang L,Deng M,Parthasarathy R,Wang L,Mongan M,Molkentin JD,Zheng Y,Xia Y

更新日期：2005-01-01 00:00:00